期刊
JOURNAL OF ENDODONTICS
卷 37, 期 9, 页码 1217-1224出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.joen.2011.05.022
关键词
Adipogenic; CD73; CD90; CD146; immunocompromised mice; inflamed periapical tissue; iPAPCs; mesenchymal stem cells; mineralized tissues; osteogenic; STRO-1
资金
- American Association of Endodontists Foundation
- National Institutes of Health [R01 DE019156]
Introduction: We previously reported the presence of mesenchymal stem/progenitor cells (MSCs) in inflamed pulp tissue. Here we asked whether MSCs also exist in inflamed periapical tissues resulting from endodontic infection. The objectives of this study were to detect the expression of MSC markers in periapical inflammatory tissues and to characterize isolated cells from these tissues. Methods: Human periapical inflammatory tissues wet? collected and processed to detect MSC marker expression by immunohistochemistry. Cells were isolated and tested for cell surface marker expression by using flow cytometry and examined for multiple differentiation potential into osteogenic and adipogenic pathways. In vivo formation of mineralized tissues was assessed in a mouse model. Results: Immunohistochemistry slowed positive staining for MSC markers STRO-1, CD90, and CD146. Isolated cells at passage 0 appeared as typical fibroblastic cells, and a few cells formed colony-forming unit-fibroblasts (CFU-Fs). After passaging, the CFU-F forming ability diminished dramatically, and the population doubling was up to 26. Flow cytometry oata showed that these cells at passage 2 expressed low levels of STRO-1 and CD146 and moderate to high levels of CD90, CD73, and CD105. At passage 6, the levels of these markers decreased. When incubated in specific differentiation medium, cells demonstrated a strong osteogenic but weak adipogenic capacity. After in vivo cell transplantation, mineralized tissues formed in immunocompromised mice. Conclusions: Human periapical inflammatory tissues expressed MSC markets, suggesting the presence of MSCs. Isolated cells exhibited typical mesenchymal cell immunopheno-type with a capacity to form mineralized matrix in vitro and in vivo. (J Endod 2011;37:1217-1224)
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