Article
Infectious Diseases
Giovanni Mergoni, Maddalena Manfredi, Pio Bertani, Tecla Ciociola, Stefania Conti, Laura Giovati
Summary: Enterococcus faecalis is a common cause of biofilm-associated infections, and two antifungal peptides showed promising activity in inhibiting E. faecalis biofilm formation. L18R demonstrated better anti-biofilm and bactericidal effects, while also showing no cytotoxicity to murine fibroblasts.
Article
Immunology
Nicole Schonbachler, Thomas Thurnheer, Pune Nina Paque, Thomas Attin, Lamprini Karygianni
Summary: This study compared the antimicrobial potential of different natural mouthrinses using in vitro and in situ biofilm models. The results showed a less pronounced effect of all mouthrinses on in situ biofilms compared to in vitro biofilms, with 0.2% chlorhexidine exhibiting the highest antimicrobial effect.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Hawraa F. Alostath, Domniki Chatzopoulou, Simon Holmes, David Gould, Gleb Sukhorukov, Michael J. Cattell
Summary: Maxillofacial surgery placement of failed fixatures can lead to advanced periodontal disease. To reduce infection rates, we coated fixatures with a new chlorhexidine surface coating. The results showed that the chlorhexidine-coated fixatures had a longer drug release time and good antibacterial effectiveness. Therefore, this technology can be used to maintain oral hygiene and prevent surgical site infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Haoran Wang, Yulu Li, Zhuo Li, Run Ma, Xiangyang Bai, Xiangjun Zhan, Kunyao Luo, Ruiying Su, Xuejiao Li, Xiaodong Xia, Chao Shi
Summary: This study found that Litsea cubeba essential oil (LC-EO) has antimicrobial activity against Cronobacter sakazakii strains, and its mechanism involves increasing reactive oxygen species production, compromising cell membrane integrity, and causing cellular damage. Additionally, LC-EO effectively inhibited C. sakazakii biofilms and showed potential as a natural antimicrobial agent in the dairy industry.
Article
Microbiology
Daniele Marra, Irene Perna, Giulio Pota, Giuseppe Vitiello, Alessandro Pezzella, Giuseppe Toscano, Giuseppina Luciani, Sergio Caserta
Summary: Microbial colonization of surfaces is a significant problem in many applications, leading to contamination and infections. This study aimed to investigate novel technologies to prevent biofilm formation on surfaces. Glass surfaces were coated with melanin-ZnO2, melanin-TiO2, and TiO2 hybrid nanoparticles using cold plasma activation. The antibiofilm properties were quantitatively characterized using Pseudomonas fluorescens AR 11 as a model organism. The results showed that the proposed surface coatings effectively prevented biofilm formation, with melanin-TiO2 demonstrating the highest efficiency. This research provides valuable support for future implementation of this technique in various applications.
Article
Microbiology
Kay Andrin Graenicher, Lamprini Karygianni, Thomas Attin, Thomas Thurnheer
Summary: The combined treatment of low concentrations of chlorhexidine (CHX) and enzymes on multispecies oral biofilms significantly reduced total colony-forming units and resulted in less dense biofilms with decreased cell numbers. This approach of disrupting matrix integrity with enzymes and antimicrobial agents shows promising potential for biofilm disruption and killing.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Fisheries
Jinseon You, Juhwan Park, Dongkyu Choi, Jiyeong Yun, Hanbyeul Choi, In Hee Lee
Summary: The study demonstrates biofilm infection in vivo using an insect model. The researchers were able to simulate implant-associated biofilm infections in Galleria mellonella larvae by injecting toothbrush bristles and methicillin-resistant Staphylococcus aureus (MRSA) into the larval hemocoel. They found that biofilm formation occurred without visible signs of infection in most of the bristle-bearing larvae for 12 hours. The study also showed that an antimicrobial peptide interfered with in vivo biofilm formation.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Polymer Science
Amr Shehabeldine, Hany El-Hamshary, Mohamed Hasanin, Ayman El-Faham, Mosaed Al-Sahly
Summary: The study successfully enhanced the antifungal and anti-biofilm activity of Griseofulvin-loaded green nanocomposite-based biopolymers against different pathogenic Candida strains. The prepared Ge-Nco was characterized in nanometer size and showed potential for inhibiting biofilm formation in Candida.
Article
Food Science & Technology
Qing-Hui Wen, Rui Wang, Si-Qi Zhao, Bo-Ru Chen, Xin-An Zeng
Summary: The study found that the citrus flavonoid naringenin inhibits the formation of biofilms in Staphylococcus aureus, possibly by reducing cell surface hydrophobicity and exopolysaccharide production that are involved in biofilm formation. In addition, naringenin also downregulates the expression of biofilm-related genes.
Article
Microbiology
Xiaolu Ma, Xiaoxi Wang, Shengna Ye, Jinnan Liu, Hong Yuan, Nan Wang
Summary: This study analyzed the pathogenic factors and drug sensitivity associated with clinical treatment of bacterial vaginosis (BV) in Northeast China. The results showed that Gardnerella vaginalis is more resistant to metronidazole than clindamycin, and neither antibiotic is able to effectively eradicate vaginal biofilms.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Paola Di Fermo, Tecla Ciociola, Silvia Di Lodovico, Simonetta D'Ercole, Morena Petrini, Laura Giovati, Stefania Conti, Mara Di Giulio, Luigina Cellini
Summary: This study demonstrated the significant antimicrobial activity of the antifungal peptide L18R against wound pathogens, with differential reduction in biomass of monomicrobial biofilms but limited impact on polymicrobial biofilms. Further studies are recommended to explore its potential role in chronic wound management in combination with conventional antibiotics or alternative treatments.
Article
Microbiology
Huan Chang Tan, Gary Shun Pan Cheung, Jeffrey Wen Wei Chang, Chengfei Zhang, Angeline Hui Cheng Lee
Summary: The study developed a reproducible biofilm model consisting of E. faecalis and P. gingivalis, showing that biofilms in co-culture models were thicker and had higher protein contents. Additionally, E. faecalis appeared to support the growth of P. gingivalis under aerobic conditions. These results reveal the interaction between the two bacterial species.
Article
Microbiology
Thomas R. Neu, Ute Kuhlicke
Summary: The use of fluorescently-labeled lectins can help analyze the structure and composition of the extracellular matrix in environmental biofilms. These lectins have high binding efficiency and provide important information about glycoconjugates. This method is valuable for studying glycoconjugates in the extracellular matrix of environmental microbial communities.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Antoine Gaillac, Romain Briandet, Elodie Delahaye, Julien Deschamps, Evelyne Vigneau, Philippe Courcoux, Emmanuel Jaffres, Herve Prevost
Summary: This study investigates the biofilm formation ability and diversity in biofilm structure of 30 different origin B. thermosphacta strains using various biofilm assays. The results reveal the presence of two major groups, low and high biofilm producers, with the high biofilm producers exhibiting flat architectural structures.
Review
Polymer Science
Uros Josic, Claudia Mazzitelli, Tatjana Maravic, Ales Fidler, Lorenzo Breschi, Annalisa Mazzoni
Summary: Incomplete and inadequate removal of endodontic biofilm during root canal treatment often leads to clinical failure. This review provides an overview of endodontic biofilm cultivation methods and discusses the effectiveness of sonic-, ultrasonic-, and Er:Yag laser-assisted biofilm removal techniques. Laser assisted agitation, as well as ultrasonic and sonic activation of the irrigants, are more efficient in biofilm removal compared to conventional irrigation. The choice of irrigant also plays a role in reducing bacterial contamination inside the root canal. Standardization of methodology and introduction of root canals with complex morphology in research are needed for better comparison and recommendations for the most efficient method in biofilm removal.
Article
Dentistry, Oral Surgery & Medicine
Sang Won Kwak, Jung-Hong Ha, Ya Shen, Markus Haapasalo, Hyeon-Cheol Kim
Summary: Glide-path preparation and coronal pre-flaring can reduce torque generation, which is advantageous for reducing stress and reactive forces to NiTi files and root dentin.
AUSTRALIAN ENDODONTIC JOURNAL
(2022)
Article
Engineering, Biomedical
He Liu, Jing Lu, Qianzhou Jiang, Markus Haapasalo, Junrong Qian, Franklin R. Tay, Ya Shen
Summary: Regenerative endodontic procedures have been widely used in clinical endodontics as a valuable adjunct to conventional strategies. Biomaterial scaffolds have shown clinical potential and remarkable advancements in regenerative endodontics.
BIOACTIVE MATERIALS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Hind Al-Zuhair, Zheng Su, He Liu, Zhejun Wang, Markus Haapasalo, Ahmed Hieawy, Yuan Gao, Ya Shen
Summary: This study compared the antibacterial effects of different agitation devices on single- and multispecies biofilms in dentin canals. The results showed that agitating NaOCl can more effectively kill biofilms. EDDY was as effective as PUI when combined with NaOCl, but the antimicrobial efficiency in the apical portion was still a concern.
Article
Dentistry, Oral Surgery & Medicine
Evan Baird, Xiangya Huang, He Liu, Ahmed Hieawy, N. Dorin Ruse, Zhejun Wang, Markus Haapasalo, Ya Shen
Summary: A dynamic model was developed to evaluate the fatigue resistance of instruments at body temperature. The study found that the combination of rotational and axial movement led to greater fatigue resistance compared to rotational movement alone. Additionally, the curvature near the apex had a greater impact on fatigue resistance than curvatures in the coronal and middle thirds.
AUSTRALIAN ENDODONTIC JOURNAL
(2023)
Article
Engineering, Biomedical
Yue Xu, Yuan You, Luyao Yi, Xiaoyi Wu, Yaning Zhao, Jian Yu, He Liu, Ya Shen, Jingmei Guo, Cui Huang
Summary: A stimuli-responsive multidrug delivery system has been developed in this study, which can prevent tooth decay and promote enamel restoration. The system can identify cariogenic conditions, intelligently release drugs, and restore the microarchitecture and mechanical properties of demineralized teeth.
BIOACTIVE MATERIALS
(2023)
Article
Engineering, Biomedical
Fushi Wang, Xinjie Cai, Ya Shen, Liuyan Meng
Summary: This review discusses the effects of various types of scaffold materials on cell-scaffold interaction in tissue engineering. It elaborates on the influence of the physical, chemical, mechanical, and biological characteristics of scaffold materials on cell-scaffold interaction. The review also examines the role of scaffold components, degradation process, and degraded products in cell-scaffold interactions. Furthermore, it summarizes the latest research progress on cell-scaffold interactions among various engineered tissues or organs in the oral and craniofacial region.
BIOACTIVE MATERIALS
(2023)
Article
Engineering, Biomedical
Jian Yu, Haolin Bian, Yaning Zhao, Jingmei Guo, Chenmin Yao, He Liu, Ya Shen, Hongye Yang, Cui Huang
Summary: As a global public health focus, oral health plays a vital role in overall health. Defected teeth with exposed dentin increase the risk of oral diseases such as hypersensitivity and inflammation. The E/PA@HMS nanosystem effectively occludes dentinal tubules, inhibits biofilm formation, induces collagen mineralization, and promotes odontogenic differentiation and mineralization of dental pulp cells. Animal experiments demonstrate its long-lasting effects in sealing tubules and inhibiting biofilm growth. This nanosystem shows promising benefits for protecting exposed dentin.
BIOACTIVE MATERIALS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Jinghao Hu, Jian Yu, He Liu, Zhejun Wang, Markus Haapasalo, Evan F. Haney, Robert E. W. Hancock, Shuli Deng, Ya Shen
Summary: This study evaluated the antimicrobial effects of commercially available mouthrinses and a D-enantiomeric peptide on oral multispecies biofilms. The results showed that DJK-5 demonstrated the highest percentage of dead bacteria and may be a potential candidate for future mouthrinses.
JOURNAL OF DENTISTRY
(2023)
Article
Dentistry, Oral Surgery & Medicine
He Liu, Stefanie Nio, Ya Shen
Summary: This study evaluated the efficacy of different concentrations of sodium hypochlorite at various exposure times and temperatures against Enterococcus faecalis biofilms in human dentinal tubules. The results showed no significant differences in effectiveness between the 2% and 5.25% sodium hypochlorite solutions at different temperatures or exposure times. Increasing the temperature or exposure time of sodium hypochlorite was found to be more important than raising its concentration to optimize its effectiveness in killing E. faecalis while minimizing potential damage.
Article
Materials Science, Multidisciplinary
Jihye Hong, Sang Won Kwak, Jung-Hong Ha, Asgeir Sigurdsson, Ya Shen, Hyeon-Cheol Kim
Summary: This study compared the fatigue resistance of files made from different heat treatment methods and surface treatment. Four prototype files were created and tested, and it was found that the file that underwent a specific temperature heat treatment with titanium coating surface treatment showed the highest fatigue resistance.
Article
Infectious Diseases
Luana Dutra de Carvalho, Bernardo Urbanetto Peres, Ya Shen, Markus Haapasalo, Hazuki Maezono, Adriana P. Manso, Frank Ko, John Jackson, Ricardo M. Carvalho
Summary: This study successfully encapsulated and controlled the release of chlorhexidine using cellulose acetate nanofibers as a drug carrier for the treatment of dental bacterial infections. The nanofiber mats produced were thin, flexible and strong, allowing for intimate contact with surfaces and effective bacterial killing.
Article
Medicine, General & Internal
Ying Li, Zhengyang Wang, Pingping Bao, Tingting Meng, Meng Liu, Huixu Li, Ya Shen, Dayong Liu, Zhi Jia, He Liu
Summary: This study aimed to compare the cleaning and disinfecting efficiency of oval-shaped root canals using XP-endo Shaper (XPS), ProTaper Next (PTN), and HyFlex CM (HCM) in combination with passive ultrasonic irrigation (PUI). Results showed that XPS outperformed PTN and HCM in disinfecting the root canals. However, removing hard tissue debris from the critical apical area remained challenging.
MEDICINA-LITHUANIA
(2023)
Article
Dentistry, Oral Surgery & Medicine
Dan Wang, Jian Yu, He Liu, Tian Zhang, Evan F. Haney, Robert E. W. Hancock, Lin Peng, Ya Shen
Summary: This study aimed to investigate the effectiveness of DJK-5-D enantiomeric antibiofilm peptide on the corrosion resistance of titanium with different surface roughness. The results showed that DJK-5 effectively enhanced the corrosion resistance of titanium while killing S. mutans biofilms, with smooth surfaces more susceptible to peptide treatment.
JOURNAL OF DENTISTRY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Kayi Lui, He Liu, Hao Wang, Xueqin Yang, Dingming Huang, Xuedong Zhou, Yuan Gao, Ya Shen
Summary: The objective of this study was to develop a customized framework for evaluating registration accuracy and measuring the untouched surface area of canal instrumentation. The results showed a significant difference between manual and automatic registration, while there was no difference in the untouched surface measure methods. There was a correlation between registration accuracy and untouched surface area.
CLINICAL ORAL INVESTIGATIONS
(2023)
Article
Medicine, General & Internal
Hui Zhang, Min Cai, Zhiguo Liu, He Liu, Ya Shen, Xiangya Huang
Summary: This study reported a novel autotransplantation technique using virtual simulation, CARP model-based rehearsed surgery, and tooth replica-guided surgery. The technique showed excellent stability and predictable outcomes in a complex case of dental rehabilitation.
MEDICINA-LITHUANIA
(2022)