Distinct hormonal regulation of Na+,K+-atpase genes in the gill of Atlantic salmon (Salmo salar L.)

It has recently become evident that maintenance of ionic homeostasis in euryhaline salmonids involves a reciprocal shift in expression of two isoforms of the gill Na+,K+-atpase alpha-subunit when the surrounding salinity changes. The present study investigated the regulation of this shift between the alpha 1a (freshwater (FW) isoform) and the all) (sea-water (SW) isoform) by cortisol, Gh, prolactin (Pr1) and Igf1. Injection with cortisol into FW salmon increased ala expression, while Gh had no effect. Conversely, both cortisol and Gh stimulated alpha 1b expression, and 1 significant synergy was observed. igf1 expression was increased by Gh in both gill and liver, and inhibited by cortisol in the liver. Gill igf1 and gh receptor expression increased in response to cortisol. Injection with Prl into SW salmon compromised their hypo-osmoregulatory performance, selectively reduced the expression of the alpha 1b isoform and decreased enzymatic Na+,K+-atpase activity in the gill. Cortisol and I'd reduced gill and liver igf1 expression, and both hormones stimulated gill igf1 receptor expression. In a short-term experiment with incubation of FW gill cell suspensions, cortisol stimulated alpha 1a and and expression, while Igf1 stimulated only alpha 1b. The data elaborate our understanding of Pr1 and (;It as being antagonists in the control of gill ion regulation, and support a dual role for Gh Involving endocrine and paracrine Igf1 action. Gh and Prl may be the decisive stimuli that direct cortisol-aided mitochondrion-rich cell development into either secretory or absorptive types. Journal of Endocrinology (2009) 203, 301-310