Article
Biochemistry & Molecular Biology
Kaiwen Xiang, Qian Yang, Mengqun Liu, Xiaodong Yang, Jifang Li, Zhishuai Hou, Haishen Wen
Summary: This study identified seven sst genes in rainbow trout and investigated the ghrh-sst-gh-igf axis in diploid and triploid trout in response to seawater challenge. The study found changes in kidney sst and sstr expressions due to osmoregulation, suggesting a pleiotropic physiology of SSTs in regulating growth and smoltification. Triploid trout showed better acclimation to the seawater environment, with up-regulated kidney igfbp5a subtypes and down-regulated igfbp5b2, indicating a conserved physiology of teleost IGFBP5a in regulating osmoregulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Mirta Resetar, Borris R. Tietcheu R. Galani, Armelle T. T. Tsamo, Ya Chen, Daniel Schachner, Stefanie Stolzlechner, Julio I. I. Mawouma Pagna, Mehdi A. A. Beniddir, Johannes Kirchmair, Verena M. M. Dirsch
Summary: This study assessed the ability of six limonoids from Trichilia prieuriana to activate the liver X receptor. One of these, flindissone, was shown to activate LXR by reporter-gene assays. Flindissone is a ring-intact limonoid, structurally similar to sterol-like LXR ligands.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Chemistry, Multidisciplinary
Ruslan Gibadullin, Brian P. Cary, Samuel H. Gellman
Summary: Class B1 GPCRs respond to extracellular polypeptide agonists by interconverting among conformational states. The activation of GLP-1R is influenced by conformational mobility of agonists, while GLP-2R is tolerant of variations in helical propensity. Comparison between the two receptors supports the conclusion that they differ in their response to variations in helical propensity near the agonist N-terminus.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Virology
David Tapia, Juan Kuznar, Rodolfo Farlora, Jose M. Yanez
Summary: The study found that rainbow trout exhibited a differential transcriptomic response to infection with two genetically distinct IPNV isolates from Chile, with RTTX infection eliciting a greater modulation of trout transcriptome. Functions related to immune and inflammatory responses were modulated in infected fish, with different regulation patterns depending on the isolate used.
Article
Fisheries
Jianmin Ye, Lan Li, Chenxi Duan, Liting Wu, Xiao Tu, Mary Ann Vogelbein, Erin Bromage, Stephen L. Kaattari
Summary: It was found that high antigen concentrations could significantly compromise IgM antibody titers and affinities in rainbow trout. Pulsed antigen cultures provided more accurate IgM antibody evaluations, and high antigen concentrations specifically suppressed high affinity B cell induction. Optimal expression of high affinity antibodies required exposure to lower concentrations of antigen.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Minfei Su, Jinan Wang, Guoqing Xiang, Hung Nguyen Do, Joshua Levitz, Yinglong Miao, Xin-Yun Huang
Summary: α(1)-adrenergic receptors (α(1)-ARs) are crucial in regulating blood pressure, cognition, and metabolism. The lack of specific agonists for all subtypes of α(1)-ARs has hindered our understanding of their physiological roles and drug development. This study reveals the molecular mechanisms underlying the discrimination between α(1A)-AR and α(1B)-AR by the agonist A61603, providing insights for the rational design of subtype-specific agonists.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Ren-Lei Ji, Ting Liu, Zhi-Shuai Hou, Hai-Shen Wen, Ya-Xiong Tao
Summary: Four mc4r genes were identified in rainbow trout, which are homologous to those of other fish and mammals. These genes have undergone different evolutionary processes. This study provides the foundation for future research on the physiological functions of mc4r paralogs and the evolution of mc4r in vertebrates.
Article
Fisheries
Mehtap Bayir, Gokhan Arslan, Erdal Ozdemir, Abdulkadir Bayir
Summary: The complexity of retinoid intracellular signaling by lipid-binding proteins is further increased in salmonids due to two whole genome duplications, resulting in potential existence of more cellular retinoic acid-binding protein/retinol-binding protein genes compared to other vertebrate species. This study in rainbow trout identified multiple crabp and rbp genes through genomic database searches and phylogenetic analyses. Experimental diets significantly modulated crabp/rbp transcripts in the liver of rainbow trout.
Article
Biochemistry & Molecular Biology
Zhi-Shuai Hou, Meng-Qun Liu, Hai-Shen Wen, Qin-Feng Gao, Zhao Li, Xiao-Dong Yang, Kai-Wen Xiang, Qian Yang, Xin Hu, Meng-Zhi Qian, Ji-Fang Li
Summary: The serotonin system plays a role in regulating physiological functions and behavioral traits in trout. In this study, we identified 39 serotonin receptor genes in rainbow trout and found that some subtypes are associated with immunomodulation and osmoregulation. This study suggests that duplicated trout serotonin receptors may have new physiological functions and potentially have pleiotropic effects in regulating immunomodulation and osmoregulation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Fisheries
Sara Comesan, Cristina Velasco, Jose L. Soengas
Summary: Results from an in vitro study suggest that rainbow trout hypothalamus responds to changes in leucine levels in a similar way to in vivo observations, activating amino acid sensing systems and GCN2 kinase. However, the activation of mTOR signaling pathway by leucine observed in vivo appears to be indirect, potentially due to the lack of connections between hypothalamus and other brain areas or changes in hormone levels such as insulin.
Article
Chemistry, Physical
Zhaoqian Su, Kalyani Dhusia, Yinghao Wu
Summary: The binding of cell surface receptors with their extracellular ligands initiates various intracellular signaling pathways, but our understanding of the cellular functions of these receptors is limited. Membrane undulations negatively affect ligand-receptor interactions, and the preassembly of membrane receptors can accelerate ligand binding kinetics and reduce noise during the process. These findings underscore the importance of membrane environments in regulating the function of membrane receptors and suggest potential applications of the simulation method in specific receptor systems involved in cell signaling.
JOURNAL OF CHEMICAL PHYSICS
(2021)
Article
Biotechnology & Applied Microbiology
Shenji Wu, Jinqiang Huang, Yongjuan Li, Zhe Liu, Lu Zhao
Summary: The study identified the regulatory role of miR-382 in rainbow trout skin pigmentation by targeting the dct gene, providing insight into the potential mechanism of regulating skin pigmentation and offering a theoretical basis for molecular breeding targeting skin color.
MARINE BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Cyrielle Billon, Sadichha Sitaula, Subhashis Banerjee, Ryan Welch, Bahaa Elgendy, Lamees Hegazy, Tae Gyu Oh, Melissa Kazantzis, Arindam Chatterjee, John Chrivia, Matthew E. Hayes, Weiyi Xu, Angelica Hamilton, Janice M. Huss, Lilei Zhang, John K. Walker, Michael Downes, Ronald M. Evans, Thomas P. Burris
Summary: Repetitive physical exercise induces physiological adaptations in skeletal muscle that improves exercise performance and is effective for the prevention and treatment of several diseases. Genetic evidence indicates that ERRs play an important role in skeletal muscle exercise capacity. Here, we report the identification of a synthetic ERR pan agonist, SLU-PP-332, that targets all three ERRs but has the highest potency for ERR alpha. SLU-PP-332 increases mitochondrial function and cellular respiration, and enhances exercise endurance.
ACS CHEMICAL BIOLOGY
(2023)
Article
Cell Biology
Jan A. Mennigen, Julianne Magnan, Kenan Touma, Carol Best, Brett M. Culbert, Nicholas J. Bernier, Kathleen M. Gilmour
Summary: Juvenile rainbow trout develop social hierarchies in constrained environments, leading to changes in energy metabolism based on social status. The somatotropic axis, which regulates metabolism and growth, may be involved in mediating these metabolic changes. This study found that social status influenced the expression of growth hormone receptor paralogues, leading to differential activation of anabolic and catabolic pathways in subordinate rainbow trout.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Chang-Qing Zhou, Wei Ka, Hui-Jun Zhang, Ya-Lan Li, Pan Gao, Rui-Jun Long, Shun-Wen Yang, Jian-Lin Wang
Summary: The study found that rainbow trout exhibit enhanced behavior, antioxidant capacity, and natural immune function in the early stages of acute heat stress, but their stress resistance decreases with increased stress intensity and duration. Non-coding RNAs play a significant role in regulating heat stress in rainbow trout, influencing metabolism, apoptosis, and immune regulation. Analysis identified key lncRNA-mRNA pairs essential for regulating acute heat stress in rainbow trout, providing insights for breeding heat-resistant varieties.