期刊
JOURNAL OF ENDOCRINOLOGY
卷 200, 期 2, 页码 177-187出版社
BIOSCIENTIFICA LTD
DOI: 10.1677/JOE-08-0234
关键词
-
资金
- European Commission [QLKI-2000-00083]
- Parthenon Trust
- Belgian Fonds National de la Recherche Scientifique
An increased vulnerability of adult beta-cells seems to be programmed in early life as adult islets from the progeny of darns fed a low-protein diet exhibited an increased apoptotic rate after cytokine stimulation. This was prevented by maternal taurine supplementation. Here, we investigated the mechanisms implicated in such all increased vulnerability and how taurine exerts its protective role. Throughout gestation and lactation, Wistar rats were fed a 20% (control (C group)) or an isocaloric 8% protein diet (recovery (K group)) supplemented or not with taurine (control + taurine and recovery + taurine groups respectively). Offspring received a 20% protein diet after weaning. Islets from 3-month-old females were isolated and cultured for 48 h before being incubated with or without cytokines for 24 h. In unstimulated islets, apoptotic rate and NO center dot secretion were higher in R than in C. Both GADD153 mRNA and protein were increased, whereas mRNA of mitochondrial gene ATPase6 was downregulated in R group compared with C. In the RT group, taurine prevented apoptosis and restored a normal NO center dot production in GADD153 as well as ATPase6 mRNA expression. After cytokines-induction, apoptosis and NO center dot secretion were still increased in R, compared with C but both parameters were normalized in the R-T group. In conclusion, a maternal low-protein diet programmes a different pattern of gene expression in islet-cells of adult progeny. Higher NO center dot production by these islets could be an important factor in the subsequent cell death. The prevention of these events by maternal taurine supplementation emphasizes the importance of taurine during endocrine pancreas development.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据