Article
Medicine, General & Internal
Carlos Arias-Cabrales, Eva Rodriguez-Garcia, Javier Gimeno, David Benito, Maria Jose Perez-Saez, Dolores Redondo-Pachon, Anna Buxeda, Carla Burballa, Marta Crespo, Marta Riera, Julio Pascual
Summary: The study investigated the role of C5a receptors in renal ischemia-reperfusion injury through in vitro models and human biopsies. The results showed that C5aR1 expression was significantly increased in acute injury, whereas C5L2 could be related to endothelial cell damage during the injury process.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Immunology
Yasutaka Kimoto, Takahiko Horiuchi
Summary: ANCA-associated vasculitis is a type of vasculitis associated with the autoantibody ANCA and mainly affects small blood vessels in various organs. It can be fatal if not treated promptly. Recent evidence suggests that C5a plays a role in the pathogenesis of this condition, and a C5aR antagonist called Avacopan has shown efficacy as a novel therapeutic option.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sandeep Silawal, Benjamin Kohl, Jingjian Shi, Gundula Schulze-Tanzil
Summary: The study found that C5a stimulation did not affect cell survival, but had a significant impact on the gene expression of C5aR, CRPs, and inflammatory cytokines at different stages. C5a may directly contribute to cell activation, thereby being involved in tendon healing and tendinopathy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Urology & Nephrology
Chris R. Toy, Huijuan Song, Haikady N. Nagaraja, Julia Scott, Jessica Greco, Xiaolan Zhang, Chack-Yung Yu, James A. Tumlin, Brad H. Rovin, Lee A. Hebert, Daniel J. Birmingham
Summary: The study reveals that the C5 gene's 2404-G allele/GG genotype is associated with lupus nephritis in black lupus patients and can lead to sharp increases in urine C5a and MAC levels during certain LN flares.
KIDNEY INTERNATIONAL REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Nicole Schaefer, Susanne Graessel
Summary: Osteoarthritis affects over 500 million people worldwide, causing significant suffering and societal cost. Chronic, low-grade inflammation is central to its pathogenesis, with the complement system possibly playing a crucial role in its development. Despite potential side effects, targeting the complement system shows promising effects in alleviating inflammatory responses in joint tissues.
Review
Immunology
Vladimir Tesar, Zdenka Hruskova
Summary: The alternative complement pathway plays an important role in the treatment of AAV. Avacopan shows similar efficacy and better safety compared to high-dose corticosteroids in the induction treatment of AAV.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Miriam Gonzalez-Gomez, Ricardo Reyes, Ma del Carmen Damas-Hernandez, Xiomara Plasencia-Cruz, Ibrahim Gonzalez-Marrero, Rafael Alonso, Aixa R. Bello
Summary: The study found that prolonged exposure to the environmental estrogenic substance bisphenol-A (BPA) can negatively affect the reproductive axis, resulting in accelerated sexual maturation and a shorter fertile life in offspring.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Felipe Silva de Franca, Isadora Maria Villas-Boas, Bruno Cogliati, Trent M. Woodruff, Edimara da Silva Reis, John D. Lambris, Denise V. Tambourgi
Summary: The study revealed that Naja annulifera venom can activate the complement system, leading to the release of lipid mediators and chemokines, and subsequent pathological reactions. Inhibition of the C5a-C5aR1 axis can mitigate the effects of complement activation. The cyclic peptide antagonist PMX205 can rescue mice from systemic reactions and prevent acute lung injury caused by complement activation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Endocrinology & Metabolism
Anello Marcello Poma, Agnese Proietti, Elisabetta Macerola, Diana Bonuccelli, Marco Conti, Alessandra Salvetti, Vincenza Dolo, Andrea Chilla, Alessio Basolo, Ferruccio Santini, Antonio Toniolo, Fulvio Basolo
Summary: This study found that the pituitary glands of lethal cases of COVID-19 had the SARS-CoV-2 genome and antigens, confirming viral infection in the pituitary gland. Activation of type I interferon signaling and increased levels of neutrophil and cytotoxic cell scores were observed in virus-positive glands, while mRNA transcripts of pituitary hormones and pituitary developmental/regulatory genes were suppressed in all COVID-19 cases.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Biochemistry & Molecular Biology
Monica Jain, Malgorzata Tecza, Todd F. Kagawa, Jakki C. Cooney
Summary: The C5a peptidase from Streptococcus pyogenes (ScpA) is a highly specific enzyme with multiple domains, with the Fn2 domain playing a critical role in enzyme function. Functional investigation of specific acidic residues on the Fn2 domain identified residues impacting binding and catalysis, supporting the presence of an exosite on Fn2.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Pharmacology & Pharmacy
Xaria X. Li, Vinod Kumar, Richard J. Clark, John D. Lee, Trent M. Woodruff
Summary: The study found that SB290157 exhibits partial agonist activity on C5aR2 and significantly dampens C5a-induced signaling in human and mouse macrophages. The drug's effects on human C3aR and C5aR1 are unclear, making it unsuitable for exploring C3aR function. This cautionary research indicates that the observed functions of SB290157 may be due to its off-target activities.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Immunology
Jovan Schanzenbacher, Joerg Koehl, Christian M. Karsten
Summary: The complement system is an important part of the innate immune system with a complex role in autoimmunity. Dysregulation of the complement system has been associated with the development of autoimmune disorders. Anaphylatoxins, such as C3a and C5a, play a crucial role in the inflammatory response during autoimmunity and regulate both innate and adaptive immune responses.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Rheumatology
Martina Mazzariol, Lucio Manenti, Augusto Vaglio
Summary: The purpose of this review is to discuss the role of the complement system in the pathogenesis of ANCA-associated vasculitis and the use of complement inhibitors in its treatment.
CURRENT OPINION IN RHEUMATOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Inez A. A. Trambas, Melinda T. T. Coughlan, Sih Min Tan
Summary: Diabetic kidney disease (DKD) affects a significant percentage of patients with diabetes and is the leading cause of end-stage renal disease (ESRD). The activation of the complement cascade, a component of the innate immune system, has been implicated in the development of diabetes and its complications. Inhibition of the complement system, particularly at the level of the C5a-receptor signaling axis, shows promise in reducing inflammation and fibrosis in DKD. This review discusses the role of the C5a/C5a-receptor axis in the pathogenesis of diabetes and kidney injuries, as well as the current status and mechanisms of action of complement therapeutics in development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Christina Johnson, Huy Quang Quach, Corinna Lau, Karin Ekholt, Terje Espevik, Trent M. Woodruff, Soren Erik Pischke, Tom Eirik Mollnes, Per H. Nilsson
Summary: Thrombin plays an important role in inflammation, but studying its effects on thromboinflammatory responses is difficult in common experimental models. In this study, the anticoagulant peptide GPRP was used to investigate the effects of thrombin on acute inflammation caused by Escherichia coli and Staphylococcus aureus. The results showed that thrombin could modulate bacterial-induced inflammation and had differential effects on cytokine production.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Microbiology
Alpana Dave, Apurwa Samarth, Roshni Karolia, Savitri Sharma, Esther Karunakaran, Lynda Partridge, Sheila MacNeil, Peter N. Monk, Prashant Garg, Sanhita Roy
Article
Immunology
Atiga Elgawidi, Muslim Idan Mohsin, Fawwaz Ali, Amyleigh Watts, Peter N. Monk, Mark S. Thomas, Lynda J. Partridge
MEDICAL MICROBIOLOGY AND IMMUNOLOGY
(2020)
Article
Multidisciplinary Sciences
Katarzyna Okurowska, Sanhita Roy, Praveen Thokala, Lynda Partridge, Prashant Garg, Sheila MacNeil, Peter N. Monk, Esther Karunakaran
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2020)
Correction
Immunology
Atiga Elgawidi, Muslim Idan Mohsin, Fawwaz Ali, Amyleigh Watts, Peter N. Monk, Mark S. Thomas, Lynda J. Partridge
MEDICAL MICROBIOLOGY AND IMMUNOLOGY
(2020)
Article
Multidisciplinary Sciences
Tanes Sangsri, Natnaree Saiprom, Alisa Tubsuwan, Peter Monk, Lynda J. Partridge, Narisara Chantratita
SCIENTIFIC REPORTS
(2020)
Article
Microbiology
Naveen Kumar Devanga Ragupathi, Dhiviya Prabaa Muthuirulandi Sethuvel, Hariharan Triplicane Dwarakanathan, Dhivya Murugan, Yamini Umashankar, Peter N. Monk, Esther Karunakaran, Balaji Veeraraghavan
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Cell Biology
Lynda J. Partridge, Lucy Urwin, Martin J. H. Nicklin, David C. James, Luke R. Green, Peter N. Monk
Summary: The spike protein of SARS-CoV-2 binds primarily to ACE2 on target cells but may also involve other receptors. Heparin and glycosaminoglycans such as heparan sulfate and dermatan sulfate can inhibit the binding of spike protein to cells, indicating potential interactions with host cells beyond ACE2.
Article
Multidisciplinary Sciences
Katherine S. Marsay, Sarah Greaves, Harsha Mahabaleshwar, Charmaine Min Ho, Henry Roehl, Peter N. Monk, Tom J. Carney, Lynda J. Partridge
Summary: Collective cell migration of the posterior lateral line primordium in zebrafish development is modulated by Cd9, which contributes to effective chemokine signaling. Cd9a and Cd9b function partially redundantly in pLLP migration, with their loss leading to delayed migration and reduced neuromast numbers. Loss of both Cd9a and Cd9b sensitizes embryos to reduced levels of Cxcr4b and Cxcl12a.
Editorial Material
Medicine, General & Internal
Naveen Kumar Devanga Ragupathi, Balaji Veeraraghavan, Esther Karunakaran, Peter N. Monk
FRONTIERS IN MEDICINE
(2022)
Article
Multidisciplinary Sciences
Sarah Greaves, Katherine S. Marsay, Peter N. Monk, Henry Roehl, Lynda J. Partridge
Summary: CD9 expression is important for sperm-egg fusion in mice, but its role in fish fertility is more complex. Knockout of cd9b in zebrafish leads to decreased egg numbers and fertilization rates. These effects are not due to alterations in primordial germ cells. The fertility defects can be rescued by crossing with wildtype partners. In vitro fertilization experiments confirm the reduced egg numbers and fertilization rates in cd9b mutants are not solely due to mating behavior. CD9 plays a role in fish fertility in both males and females.
Article
Microbiology
Luke R. R. Green, Rahaf Issa, Fawzyah Albaldi, Lucy Urwin, Ruth Thompson, Henna Khalid, Claire E. E. Turner, Barbara Ciani, Lynda J. J. Partridge, Peter N. N. Monk
Summary: This article investigates the role of the host membrane adapter protein CD9 in Staphylococcus aureus adhesion to epithelial cells. The study demonstrates that CD9 is essential for syndecan-mediated bacterial adhesion and that it coordinates with fibronectin to promote bacterial adherence and internalization. Insights from this research may lead to the development of alternative anti-adhesion treatments for staphylococcal infections.
Article
Multidisciplinary Sciences
Dhammika Leshan, Aye Mya Sithu Shein, Cameron Hurst, Peter N. Monk, Parichart Hongsing, Phatthranit Phattharapornjaroen, William Graham Fox Ditcham, Puey Ounjai, Thammakorn Saethang, Naphat Chantaravisoot, Pattama Wapeesittipan, Sirirat Luk-in, Sasipen Sae-Joo, Sumanee Nilgate, Ubolrat Rirerm, Chanikan Tanasatitchai, Naris Kueakulpattana, Matchima Laowansiri, Tingting Liao, Rosalyn Kupwiwat, Rojrit Rojanathanes, Natharin Ngamwongsatit, Somkanya Tungsanga, Asada Leelahavanichkul, Naveen Kumar Devanga Ragupathi, Vishnu Nayak Badavath, S. M. Ali Hosseini, Talerngsak Kanjanabuch, Nattiya Hirankarn, Robin James Storer, Longzhu Cui, Mohan Amarasiri, Hitoshi Ishikawa, Paul G. Higgins, Stephen M. Stick, Anthony Kicic, Tanittha Chatsuwan, Shuichi Abe
Summary: The novel aztreonam-Ca-EDTA or ceftazidime-avibactam-Ca-EDTA combinations show favorable efficacy and safety in treating carbapenemase-positive Klebsiella pneumoniae (CPKp) infections.
Article
Medicine, Research & Experimental
Aye Mya Sithu Shein, Dhammika Leshan Wannigama, Cameron Hurst, Peter N. Monk, Mohan Amarasiri, Vishnu Nayak Badavath, Phatthranit Phattharapornjaroen, William Graham Fox Ditcham, Puey Ounjai, Thammakorn Saethang, Naphat Chantaravisoot, Wanwara Thuptimdang, Sirirat Luk-in, Sumanee Nilgate, Ubolrat Rirerm, Chanikan Tanasatitchai, Naris Kueakulpattana, Matchima Laowansiri, Tingting Liao, Rosalyn Kupwiwat, Rojrit Rojanathanes, Natharin Ngamwongsatit, Arsa Thammahong, Hitoshi Ishikawa, Daniel Pletzer, Asada Leelahavanichkul, Naveen Kumar Devanga Ragupathi, Pattama Wapeesittipan, S. M. Ali Hosseini Rad, Talerngsak Kanjanabuch, Robin James Storer, Kazuhiko Miyanaga, Longzhu Cui, Hiroshi Hamamoto, Paul G. Higgins, Anthony Kicic, Tanittha Chatsuwan, Parichart Hongsing, Shuichi Abe
Summary: This study tested the efficacy of a combination therapy using phage and an adjuvant in treating multidrug-resistant Pseudomonas aeruginosa infections. The results showed that intranasal inhalation of phage along with CaEDTA successfully resensitized the bacteria to specific antibiotics, indicating its potential as a therapy for respiratory tract infections.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Meeting Abstract
Respiratory System
Dhammika Leshan Wannigama, Cameron Hurst, Peter N. Monk, Shuichi Abe, Gunter Hartel, Paul G. Higgins, Stephen M. Stick, Anthony Kicic, Jane Davies, Tanittha Chatsuwan
Review
Cell Biology
Lucy Urwin, Katarzyna Okurowska, Grace Crowther, Sanhita Roy, Prashant Garg, Esther Karunakaran, Sheila MacNeil, Lynda J. Partridge, Luke R. Green, Peter N. Monk