期刊
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
卷 660, 期 1, 页码 8-13出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jelechem.2011.05.012
关键词
Biosensor; Reverse iontophoresis; Noninvasive monitoring; Glucose
资金
- NSFC [60576049]
- Beijing Municipal Science, Technology Commission [Z0006302040191]
- Hi-Tech R&D Program of China [2005AA404220]
- Specialized Research Fund for the Doctoral Program from Higher Education of China [20090131120041]
- Shandong Province Outstanding Research Award Fund for young scientist [BS2010DX018]
A continuous, noninvasive glucose monitoring method is necessary for diabetic patients to reduce the risk of hypoglycemia. This paper was to develop a bifunctional biosensor for the simultaneous extracting and detecting the subcutaneous glucose by reverse iontophoresis. The biosensor was constructed by a tri-electrode planar configuration and multi-layer sensitive membrane composed of mediator, enzyme and cross linking agents. A hydrogel membrane was deposited on the top of the device to act as a collected reservoir of the extracted glucose. The electrochemical characteristic of the amperometric biosensor was evaluated using cyclic voltammetry at applied potentials from -0.2 V to 0.6 V vs. Ag vertical bar AgCl electrode. The glucose biosensor showed a high sensitivity of 14.24 nA mu M-1 cm(-2) at a low applied potential (-0.1 V vs. Ag vertical bar AgCl). Finally, the biosensor was used for noninvasive glucose monitoring by reverse iontophoresis using the naked mouse skin as the model. An excellent linear relationship (r(2) = 0.99) was found between the subcutaneous glucose concentration (0-18 mM) and the biosensor current response. The results suggest that the biosensor was suitable for subcutaneous glucose monitoring by reverse iontophoresis. (C) 2011 Elsevier B.V. All rights reserved.
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