期刊
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
卷 659, 期 1, 页码 1-5出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jelechem.2011.04.003
关键词
Cholesterol oxidase; Horseradish peroxidase; Layered double hydroxides; Chitosan; Cholesterol; Biosensor
资金
- National Natural Science Foundation of China [20905011, 21010102071]
- Natural Science Foundation of Jiangsu [BK2010396]
- University science research project of Jiangsu Province [09KJB150015]
- State Key Laboratory of Bioelectronics, Southeast University
- Southeast University Creative Foundation [3207040501]
Using layered double hydroxides (LDHs)-chitosan (Chit) composite as enzyme immobilization matrix, the developments of cholesterol biosensor were achieved by both electrooxidation and electroreduction of the enzymatic-generated hydrogen peroxide based on cholesterol oxidase (ChOx) and horseradish peroxidase (HRP). Amperometric detection of cholesterol was evaluated by holding the modified electrode at 0.55 V and 0 V (vs. SCE), for ChOx/LDHs-Chit/Pt and ChOx/HRP/LDHs-Chit/GCE, respectively. The linear dynamic ranges of anodic and cathodic detections of cholesterol were between 5 x 10(-7) to 8 x 10(-4) M and 4 x 10(-8) to 6 x 10(-4) M, respectively. The detection limits were determined to be of 1 x 10(-7) and 4 x 10(-8) M with anodic and cathodic processes, respectively. At potential of 0 V, ChOx/HRP/LDHs-Chit/GCE bienzymatic mediated system exhibited excellent selectivity and stability. (C) 2011 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据