期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 175, 期 6, 页码 2895-2906出版社
SPRINGER
DOI: 10.1007/s12010-014-1461-6
关键词
beta-Carotene; Chlorophyll; Dunaliella salina; Dunaliella bardawil; Bicarbonate; Saturated fatty acids
资金
- CSIR (Council for Scientific and Industrial Research), India
This study aimed to explore the effect of sodium bicarbonate (0-200 mM) on the production of beta-carotene and lipid content in Dunaliella salina and Dunaliella bardawil. Total carotenoid and chlorophyll content were determined at regular intervals by a UV-VIS spectrophotometer. The beta-carotene and lipid contents were analyzed using high-performance liquid chromatography (HPLC) and gas chromatography coupled with mass spectrometry (GC-MS). The HPLC results revealed a twofold increase of beta-carotene in D. salina and D. bardawil cultures grown with sodium bicarbonate. Moreover, total fatty acid profiles from GC-MS indicated a maximum relative percentage of saturated fatty acids (tetradecanoic acid, 10,13-diethyl, methyl ester and methyl 16-methyl-heptadecanoate) compared to polyunsaturated fatty acids in both algae. Our results indicate that the optimum concentration of bicarbonate (100 to 150 mM) was required to stimulate a positive effect on beta-carotene production as well as the lipid profile in Dunaliella sp.
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