Article
Materials Science, Biomaterials
Peng Liu, Weifei Zhang, Jiapeng Deng, Yien Zheng, Jian Weng, Fei Yu, Deli Wang, Ming Zheng, Bin Kang, Hui Zeng
Summary: A chain-shattering polymeric SO2 prodrug micelle system was developed for effective SO2 based gas therapy. The system enables efficient delivery and on-demand stimuli triggered release at tumor sites, with low tissue toxicity.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Engineering, Biomedical
Ye Liu, Maolin Jiang, Zhongyi Zhao, Nianhua Wang, Kewei Wang, Youyong Yuan
Summary: This study presents a smart nanoplatform CF@NDOX, which significantly up-regulates the expression of tumor-associated enzyme NQO1 through cyclic amplification of intracellular ROS, leading to efficient activation of the prodrug DOX and enhanced chemo-immunotherapy.
ACTA BIOMATERIALIA
(2023)
Article
Multidisciplinary Sciences
Wenting Guo, Xiaojia Li, Jingyu Fan, Hongwei Li, Yan Wen, Chunyan Meng, Haitao Chen, Zhipeng Zhao, Yuling Zhang, Yushen Du, Baixing Wu
Summary: In this study, the crystal structures of VCZ and its complex with 5-FU were determined to investigate the substrate binding, catalytic reaction, and conformational changes of VCZ for converting IC into uracil. The therapeutic effect of VCZ protein in colorectal cancer cell lines was also evaluated. These findings provide insights for optimizing the ICD/5-FIC pair and improving its application in clinical trials.
Article
Chemistry, Multidisciplinary
Julia E. Rosenberger, Yixin Xie, Yinzhi Fang, Xinyi Lyu, William S. Trout, Olga Dmitrenko, Joseph M. Fox
Summary: Ligand-directed catalysts are developed for live-cell, photocatalytic activation of bioorthogonal chemistry. Silarhodamine (SiR) dyes, originally used as biological fluorophores, serve as photocatalysts with high cytocompatibility and minimal singlet oxygen production. Computation is used to design a new class of redox-activated photocage for releasing phenol or microtubule-destabilizing agent. In model and cellular studies, successful uncaging and microtubule depolymerization are achieved with low concentrations of photocage and SiR-H dye.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Polymer Science
Shubhra Goel, Tejinder Kaur, Neetu Singh, Josemon Jacob
Summary: This article reports the preparation of novel D-galactose based hydrogels for biomedical applications. The hydrogels exhibit porous morphology with tunable properties such as swelling ratio and drug release. The cytocompatibility of the material shows its potential for various biomedical applications.
EUROPEAN POLYMER JOURNAL
(2021)
Article
Pharmacology & Pharmacy
Jingjing Ye, Min Gong, Jian Song, Shu Chen, Qinghan Meng, Rui Shi, Liqun Zhang, Jiajia Xue
Summary: Chronic inflammation inhibits tissue regeneration, and there is a high demand for inflammation-responsive scaffolds to regulate chronic inflammation. By utilizing an inflammation-sensitive prodrug and nanofiber scaffolds, controlled release of anti-inflammatory drugs can be achieved, effectively inhibiting chronic inflammation.
Article
Chemistry, Multidisciplinary
Man Li, Longbing Ling, Qing Xia, Xinsong Li
Summary: Disulfide crosslinked micelles composed of di-lipoyl-glycerophosphorylcholine were developed as redox-responsive drug carriers, demonstrating high stability and reduction-responsive drug release profile, with potential applications in the delivery of paclitaxel and other anti-cancer drugs.
Article
Polymer Science
M. Margarida Cardoso, Ines N. Peca, Telma Lopes, Rui Gardner, A. Bicho
Summary: DWNPs were produced with PLGA and PLLA containing doxorubicin as a model drug. Optical and fluorescence microscopy were used to examine the inner morphology and drug distribution of the double-walled microparticles. The study showed that the double-walled nanoparticles exhibited sustained drug release and the PLLA layer acted as a rate control barrier.
Review
Materials Science, Biomaterials
Li Qiao, Huishu Yang, Shijie Gao, Le Li, Xianjun Fu, Qingcong Wei
Summary: Nanodrug delivery systems combined with self-assembly technology are attracting increasing attention due to their advantages, and are expected to have potential therapeutic value in the field of biomedicine in the future.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Chemistry, Physical
Cui Lu, Sheng Zhang, Wei Song, Jia Liu, Xiulai Chen, Liming Liu, Jing Wu
Summary: The study successfully achieved efficient production of D-phenylalanine from L-phenylalanine through the engineering of a tri-enzymatic cascade system, providing a new bio-based approach for industrial production of D-phenylalanine from cheap amino acids.
Article
Biology
Noreen Samad, Muhammad Abubaker Hassan Azdee, Imran Imran, Tanveer Ahmad, Faleh Alqahtani
Summary: This study found that the fruit peel of Mukia madrespatana has powerful antioxidative and neuromodulatory properties, which can improve behavioral and cognitive impairments caused by aging.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Article
Engineering, Biomedical
Sophie M. Coulter, Sreekanth Pentlavalli, Lalitkumar K. Vora, Yuming An, Emily R. Cross, Ke Peng, Kate McAulay, Ralf Schweins, Ryan F. Donnelly, Helen O. McCarthy, Garry Laverty
Summary: Eradicating HIV/AIDS by 2030 is a central goal of the World Health Organization. Patient adherence to complicated dosage regimens remains a key barrier. This paper presents an alternative platform, an injectable in situ forming hydrogel implant to deliver a model antiretroviral drug (zidovudine [AZT]) over 28 days.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Agriculture, Multidisciplinary
Joanna Afokai Quaye, Daniel Ouedraogo, Giovanni Gadda
Summary: The commercial food and l-amino acid industries use bioengineered d-amino acid oxidizing enzymes to detect and remove d-amino acid contaminants. This study successfully engineered a faster biocatalyst through mutation and kinetic methods, which has potential applications in industrial settings.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Polymer Science
Malihe Pooresmaeil, Hassan Namazi, Roya Salehi
Summary: In this research, a new double-responsive nanogel with pH and temperature sensitivity, as well as photoluminescent properties, was designed and fabricated. The success in fabrication was confirmed using common characterization techniques. The potential of the nanogel as a targeted drug carrier was evaluated, and it showed good performance in loading and controlled release of anticancer drugs. The nanogel also demonstrated good biocompatibility and bioimaging potential, making it a promising medical nanovehicle for cancer cells monitoring and co-drug delivery.
EUROPEAN POLYMER JOURNAL
(2022)
Article
Nanoscience & Nanotechnology
Elena Rosini, Noemi Antonella Volpi, Barbara Ziffels, Annalisa Grimaldi, Silvia Sacchi, Dario Neri, Loredano Pollegioni
Summary: A chimeric protein, F8(scFv)-DAAO-Q144R, was designed to target the EDA of fibronectin on tumor neovasculature. This recombinant protein showed high solubility, specific activity, and thermostability, with strong cytotoxicity to tumor cells. The conjugate has potential for selective antitumor oxidative therapy and minimal damage to normal tissues.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Antony J. Fairbanks
Summary: 2-Chloro-1,3-dimethylimidazolinium chloride (DMC) and its derivatives are valuable for selectively activating the anomeric center of unprotected reducing sugars in aqueous solution, allowing for efficient synthesis of glycosides and glycoconjugates without traditional protecting group manipulations. This mini-review explores the development and recent applications of DMC in protecting group-free synthesis.
CARBOHYDRATE RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Moritz Lasse, Anja R. Stampfli, Thomas Orban, Roshit K. Bothara, Juliet A. Gerrard, Antony J. Fairbanks, Neil R. Pattinson, Renwick C. J. Dobson
Summary: The study identified acrolein modifications on hemoglobin using mass spectrometry and found that these modifications have minimal impact on the secondary structure of hemoglobin. Acrolein modifications promote the formation of crosslinked octamers and alter the response to O2 binding. The study provides qualitative evidence for potential targeted mass spectrometric or immunometric assays for acrolein modified hemoglobin as an oxidative stress marker.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Article
Biochemistry & Molecular Biology
Michael J. Currie, Lavanyaa Manjunath, Christopher R. Horne, Phillip M. Rendle, Ramaswamy Subramanian, Rosmarie Friemann, Antony J. Fairbanks, Andrew C. Muscroft-Taylor, Rachel A. North, Renwick C. J. Dobson
Summary: The study found that the enzyme NanE may use a novel substrate-assisted proton displacement mechanism for catalysis, by re-evaluating the central role of Glu180 and the catalytic lysine, and identifying several active-site residues related to the mechanism. This discovery could be helpful in developing inhibitors for the enzyme or producing biocatalysts capable of changing the stereochemistry of molecules through enzyme engineering.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Correction
Biochemistry & Molecular Biology
Tim A. Mollner, Patrick G. Isenegger, Brian Josephson, Charles Buchanan, Lukas Lercher, Daniel Oehlrich, D. Flemming Hansen, Shabaz Mohammed, Andrew J. Baldwin, Veronique Gouverneur, Benjamin G. Davis
NATURE CHEMICAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tim A. Mollner, Patrick G. Isenegger, Brian Josephson, Charles Buchanan, Lukas Lercher, Daniel Oehlrich, D. Flemming Hansen, Shabaz Mohammed, Andrew J. Baldwin, Veronique Gouverneur, Benjamin G. Davis
Summary: Post-translational formation of boronoalanine in proteins through C beta-B gamma bond allows for mild, direct, and site-selective access, enabling tunable interactions and de novo function.
NATURE CHEMICAL BIOLOGY
(2021)
Correction
Biochemistry & Molecular Biology
Moritz Lasse, Anja R. Stampfli, Thomas Orban, Roshit K. Bothara, Juliet A. Gerrard, Antony J. Fairbanks, Neil R. Pattinson, Renwick C. J. Dobson
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2022)
Article
Biochemistry & Molecular Biology
Isidro Cobo, M. Isabel Matheu, Sergio Castillon, Benjamin G. Davis, Omar Boutureira
Summary: This study investigates the scope and limitations of common bioconjugation techniques for generating well-defined glycodendriproteins, as synthetic functional mimics of glycoproteins. A diverse reactive platform was designed using recombinant proteins containing non-canonical amino acids, resulting in the successful synthesis of multivalent carbohydrate moieties attached to proteins.
Article
Multidisciplinary Sciences
Tim A. Mollner, Andrew M. Giltrap, Yibo Zeng, Yana Demyanenko, Charles Buchanan, Daniel Oehlrich, Andrew J. Baldwin, Daniel C. Anthony, Shabaz Mohammed, Benjamin G. Davis
Summary: Biomolecule environments have the potential to enhance chemistries and mediate self-modification. This study demonstrates a system that combines protein-enhanced chemistry with tag modification to enable synthetic reductive protein chemistries. This method allows for simple and site-selective cleavage of proteins under aqueous conditions and in cell lysates, making it a valuable tool for precise chemical proteolysis and protein modification.
Article
Multidisciplinary Sciences
Charles J. Buchanan, Ben Gaunt, Peter J. Harrison, Yun Yang, Jiwei Liu, Aziz Khan, Andrew M. Giltrap, Audrey Le Bas, Philip N. Ward, Kapil Gupta, Maud Dumoux, Tiong Kit Tan, Lisa Schimaski, Sergio Daga, Nicola Picchiotti, Margherita Baldassarri, Elisa Benetti, Chiara Fallerini, Francesca Fava, Annarita Giliberti, Panagiotis Koukos, Matthew J. Davy, Abirami Lakshminarayanan, Xiaochao Xue, Georgios Papadakis, Lachlan P. Deimel, Virginia Casablancas-Antras, Timothy D. W. Claridge, Alexandre M. J. J. Bonvin, Quentin J. Sattentau, Simone Furini, Marco Gori, Jiandong Huo, Raymond J. Owens, Christiane Schaffitzel, Imre Berger, Alessandra Renieri, James H. Naismith, Andrew J. Baldwin, Benjamin G. Davis
Summary: This study utilizes universal saturation transfer analysis to reveal the interaction between the novel coronavirus and glycan compounds, and identifies protein mutations related to pathogenicity and zoonosis.
Article
Biochemical Research Methods
Patrick G. Isenegger, Brian Josephson, Ben Gaunt, Matthew J. Davy, Veronique Gouverneur, Andrew J. Baldwin, Benjamin G. Davis
Summary: This article describes a robust, mild and fast method for the posttranslational, site-directed fluorination of protein sidechains, which can be detected by F-19-based magnetic resonance methods. This chemical editing method is stable, mild, fast and highly efficient, and can be used by non-experts to prepare fluorinated proteins within 24 hours without the need for bespoke biological systems.
Article
Chemistry, Organic
Xin Qiu, Daniel Chong, Antony J. Fairbanks
Summary: Unprotected sugars can be selectively acetylated in aqueous solution by stirring with acetic anhydride and a weak base, such as sodium carbonate. This reaction specifically targets the anomeric hydroxyl group of mannose, 2-acetamido, and 2-deoxy sugars and can be conducted on a large scale. However, over-reaction and the formation of product mixtures can occur due to competitive intramolecular migration of the 1-O-acetate to the 2-hydroxyl group when these two substituents are in cis configuration.
Article
Chemistry, Multidisciplinary
Benjamin G. Davis, Xuanxiao Chen, Brian Josephson
Summary: Methods to directly modify proteins post-translationally have potential for creating and studying protein post-translational modifications. Recent developments in single electron/radical chemistry have allowed for site-specific editing of proteins. This review gives an overview of current methods that install such edits, focusing on radical C-C bond formation and its compatibility with proteins and cells, as well as the novel applications afforded by these methods. Despite technical challenges, radical C-C bond formation on proteins is a promising and rapidly growing field in chemical biology.
ACS CENTRAL SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Xia-Ping Fu, Yizhi Yuan, Ajay Jha, Nikita Levin, Jack Ren, Dimitrios Mamalis, Shabaz Mohammed, Benjamin G. Davis, Andrew M. Giltrap
Summary: Chemical post-translational methods allow editing of proteins without genetic intervention. A light-mediated desulfurative method can generate site-selectively edited proteins with full retention of native stereochemistry from a natural amino acid precursor. This methodology has great potential in exploring protein side-chain diversity and function and constructing useful bioconjugates.
ACS CENTRAL SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Xin Qiu, Anna L. Garden, Antony J. Fairbanks
Summary: In this study, a new method for synthesizing glycosides was discovered. Unprotected 2-acetamido sugars were directly converted into their oxazolines in aqueous solution using 2-chloro-1,3-dimethylimidazolinium chloride (DMC) and a suitable base. Freeze drying and acid-catalyzed reaction with an alcohol as solvent yielded 1,2-trans-glycosides in good yield. Alternatively, using an aprotic solvent system and acidic activation with an excess of unprotected glycoside as a glycosyl acceptor led to the stereoselective formation of 1,2-trans-linked disaccharides without any protecting group manipulations. Reactions using aryl glycosides as acceptors were completely regioselective, producing only (1->6)-linked disaccharides.
Meeting Abstract
Biochemistry & Molecular Biology
Sivasinthujah Srikokulan, Antony Fairbanks
