Article
Plant Sciences
Nazish Annum, Moddassir Ahmed, Mark Tester, Zahid Mukhtar, Nasir Ahmad Saeed
Summary: This study investigated the role of Phospholipase C Isoform 5 (PLC5) in thermotolerance in Arabidopsis thaliana. The results showed that over-expression of PLC5 contributes to heat stress tolerance and maintains photosynthetic activity, with effects on root development.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Naoyuki Sotta, Yukako Chiba, Kyoko Miwa, Seidai Takamatsu, Mayuki Tanaka, Yui Yamashita, Satoshi Naito, Toru Fujiwara
Summary: The study revealed the translation regulation mechanism in response to boron conditions in Arabidopsis thaliana shoots, with uORFs and AUG-stops playing important roles in this process.
Article
Developmental Biology
Yingzhang Huang, Limeng Zhou, Chunhui Hou, Dianjing Guo
Summary: This study reveals the proteome landscape of Arabidopsis early embryogenesis through nanoproteomic analysis, and identifies gene enrichment related to ribosome biogenesis and auxin signaling. The positive correlation between mRNA and protein is confirmed by integrating RNA-sequencing data. This work provides valuable insights for further investigation into plant embryo development.
Article
Environmental Sciences
Youngjae Pyo, Heewon Moon, Adji Baskoro Dwi Nugroho, Seong Wook Yang, Il Lae Jung, Dong-Hwan Kim
Summary: Strontium, particularly its radioactive isotope Sr-90, poses a global threat to the environment and human health. The molecular mechanisms underlying plant response to Sr toxicity remain largely unknown. This study used RNA-seq analysis to compare gene expression profiles between control and Sr-treated plants, revealing that the JA biosynthesis and/or signaling pathway may play important roles in plant response to Sr stress, maintaining proper growth and development.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Plant Sciences
Zirui Wang, Dawood Khan, Leilin Li, Jing Zhang, Zed Rengel, Baige Zhang, Qi Chen
Summary: Hydrogen acts as a signaling molecule in Arabidopsis, regulating stomatal closure by promoting the generation of reactive oxygen species and nitric oxide. The GPA1 gene is found to be necessary for hydrogen-rich water-induced stomatal closure in Arabidopsis.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Umme Amara, Jianzhong Hu, Jing Cai, Hunseung Kang
Summary: N6-methyladenosine (m6A) is the most abundant modification in eukaryotic mRNAs and is regulated by m6A writers, erasers, and readers. The study identified FLK as a novel mRNA m6A reader protein that directly binds the m6A site in the FLC transcripts to repress FLC levels. This finding establishes a molecular link between FLK-mediated recognition of m6A modifications and floral transition in Arabidopsis.
Article
Plant Sciences
Xinyu Lu, Zitong Yang, Wen Song, Jinlu Miao, Hanqing Zhao, Peiyun Ji, Tianli Li, Jierui Si, Zhiyuan Yin, Maofeng Jing, Danyu Shen, Daolong Dou
Summary: In this study, a class of FYVE domain-containing proteins was systematically investigated, and one secreted protein, PsFYVE1, was found to promote Phytophthora infection in both tobacco and soybean. It was also discovered that PsFYVE1 and NbRZ-1A co-regulated pre-mRNA alternative splicing and transcription levels of plant immunity-related genes, promoting disease development.
Article
Plant Sciences
Bhavika Tiwari, Kristin Habermann, M. Asif Arif, Oguz Top, Wolfgang Frank
Summary: This study investigated the transcriptome data of Arabidopsis plants subjected to high light conditions, identifying a large number of high light responsive miRNAs and sRNAs derived from NAT gene pairs, lncRNAs, and TAS transcripts. The study provides a deeper understanding of sRNA-dependent regulatory networks in high light acclimation.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Jing Cai, Jianzhong Hu, Umme Amara, Su Jung Park, Yuxia Li, Daesong Jeong, Ilha Lee, Tao Xu, Hunseung Kang
Summary: FIONA1 is a novel mRNA N-6-methyladenosine methyltransferase that regulates floral transition by influencing the splicing of FLC, a key floral repressor, and the stability of SPL3and SEP3, floral activators, in Arabidopsis. Our study confirmed the function of FIONA1 as an m(6)A methyltransferase and suggested a close molecular link between FIONA1-mediated m(6)A methylation and the splicing of FLC and the destabilization of SPL3 and SEP3 in flowering time control.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
He Zhang, Shuai Liu, Xinyu Li, Lijuan Yao, Hongyang Wu, Frantisek Baluska, Yinglang Wan
Summary: A circRNA named ag-circRBCS was identified in Arabidopsis, showing low expression levels compared to RBCS genes. Delivery of ag-circRBCS into Arabidopsis seedlings resulted in significant depression of RBCS expression. These findings suggest that ag-circRBCS may play a role in fine-tuning the expression of RBCS genes in Arabidopsis.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Le Nguyen Tieu Ngoc, Su Jung Park, Jing Cai, Trinh Thi Huong, Kwanuk Lee, Hunseung Kang
Summary: Ribosomal RNA (rRNA) methylation is crucial for ribosome assembly and function, which are important for plant growth and stress responses. In this study, a new rRNA methyltransferase, RsmD, was identified in Arabidopsis chloroplasts, and it plays a role in plant adaptation to cold stress.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Plant Sciences
Hong-Sheng Liao, Chien-Chih Yang, Ming-Hsiun Hsieh
Summary: The study reveals that HDA15-mediated histone modification regulates anthocyanin accumulation in response to N deficiency and other stresses in Arabidopsis. Different stresses activate anthocyanin biosynthesis through distinct regulatory modules.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Chemistry, Multidisciplinary
Xiao-Meng Tang, Tian -Tian Ye, Xue-Jiao You, Xiao-Ming Yin, Jiang -Hui Ding, Wen-Xuan Shao, Meng -Yuan Chen, Bi-Feng Yuan, Yu-Qi Feng
Summary: In this study, a method for purifying plant ribosomal RNA (rRNA) was proposed and modifications in plant 18S rRNA and 25S rRNA were characterized using LC-ESI-MS/MS. The study revealed the presence of new modifications in plant rRNAs, including 2 modifications in 18S rRNA and 4 modifications in 25S rRNA. Exposure to cadmium induced significant changes in rRNA modifications, highlighting the importance of rRNA modifications in response to environmental stress. The discovery of new modifications expands our understanding of plant rRNA modifications and enhances the investigation of the functional roles of plant ribosomes in gene expression regulation.
CHINESE CHEMICAL LETTERS
(2023)
Article
Plant Sciences
Selin Buelbuel, Yasuhito Sakuraba, Mastoureh Sedaghatmehr, Mutsumi Watanabe, Rainer Hoefgen, Salma Balazadeh, Bernd Mueller-Roeber
Summary: BBX14 is a negative regulator of nitrogen starvation-induced leaf senescence and is directly regulated by PIF4 and MYB44. It directly regulates the transcription of EIN3, which is involved in nitrogen signaling and leaf senescence.
Article
Biology
Yuange Duan, Ye Xu, Fan Song, Li Tian, Wanzhi Cai, Hu Li
Summary: C-to-U RNA editing in plants is adaptive and helps in restoring unfavorable genomic sequences, while A-to-I RNA editing in insects like Drosophila and honeybee is diversifying and increases proteomic diversity in a spatial-temporal manner.
Article
Biochemistry & Molecular Biology
Salvador I. Drusin, Rodolfo M. Rasia, Diego M. Moreno
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
(2020)
Article
Multidisciplinary Sciences
Arantxa M. L. Rojas, Salvador I. Drusin, Uciel Chorostecki, Julieta L. Mateos, Belen Moro, Nicolas G. Bologna, Edgardo G. Bresso, Arnaldo Schapire, Rodolfo M. Rasia, Diego M. Moreno, Javier F. Palatnik
NATURE COMMUNICATIONS
(2020)
Article
Biophysics
Ricarda Torner, Faustine Henot, Rida Awad, Pavel Macek, Pierre Gans, Jerome Boisbouvier
Summary: Prefoldin acts as a co-chaperonin for Hsp60 in protein folding, with successful assignment of chemical shifts for the prefoldin from Pyrococcus horikoshii. The protein assembly captures and transfers client proteins for refolding, playing a key role in proteostasis.
BIOMOLECULAR NMR ASSIGNMENTS
(2021)
Article
Biochemistry & Molecular Biology
Faustine Henot, Rime Kerfah, Ricarda Torner, Pavel Macek, Elodie Crublet, Pierre Gans, Matthias Frech, Olivier Hamelin, Jerome Boisbouvier
Summary: The study presents a new synthetic precursor for connecting leucine and valine pro-S methyl moieties to backbone atoms, facilitating quantitative NMR studies of large proteins. This optimized precursor allows for direct transfer of backbone assignment to corresponding methyl groups without correction of H-1/H-2 isotopic shifts on C-13 resonances, as demonstrated on the N-terminal domain of human HSP90.
JOURNAL OF BIOMOLECULAR NMR
(2021)
Article
Environmental Sciences
Fiorella Masotti, Betiana S. Garavaglia, Ainelen Piazza, Paula Burdisso, Silvia Altabe, Natalia Gottig, Jorgelina Ottado
Summary: Thirteen bacterial strains capable of degrading glyphosate were isolated from environmental samples in Argentine soybean crop areas, with Achromobacter, Agrobacterium, and Ochrobactrum showing the best degradation performance. The genome of the highly efficient GBH degrader Agrobacterium tumefaciens CHLDO was sequenced, revealing the presence of a phn cluster responsible for phosphonate metabolization. Expression analysis of the phn genes in A. tumefaciens CHLDO suggests their strong participation in GBH degradation, highlighting the potential for bioremediation techniques in glyphosate-contaminated environments.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Multidisciplinary Sciences
Matthieu P. M. H. Benoit, Ana B. Asenjo, Mohammadjavad Paydar, Sabin Dhakal, Benjamin H. Kwok, Hernando Sosa
Summary: The study presents the structural changes of KIF14 bound to microtubules, revealing the crucial roles of microtubule binding, nucleotide species, and the neck-linker domain in the transition between major conformations of the motor domain. Additionally, the position of the neck-linker regulates hydrolysis rather than nucleotide binding step.
NATURE COMMUNICATIONS
(2021)
Article
Virology
Juline Poirson, Irina Paula Suarez, Marie-Laure Straub, Alexandra Cousido-Siah, Paul Peixoto, Eric Hervouet, Anne Foster, Andre Mitschler, Noella Mukobo, Yassmine Chebaro, Dominique Garcin, Sevda Recberlik, Christian Gaiddon, Daniele Altschuh, Yves Nomine, Alberto Podjarny, Gilles Trave, Murielle Masson
Summary: Persistent HPV infections can lead to various cancers by interacting with different cellular targets that affect interferon-beta expression.
JOURNAL OF VIROLOGY
(2022)
Article
Multidisciplinary Sciences
Byron Hunter, Matthieu P. M. H. Benoit, Ana B. Asenjo, Caitlin Doubleday, Daria Trofimova, Corey Frazer, Irsa Shoukat, Hernando Sosa, John S. Allingham
Summary: This study reveals how Kinesin-8 alternates between promotility and pro-microtubule-depolymerization states by regulating its tubulin structure through the loop-2 region. Structural changes induced by curved microtubule ends and straight protofilaments result in different functional outcomes.
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Santiago Cuesta, Paula Burdisso, Amir Segev, Said Kourrich, Vanessa Sperandio
Summary: The study on the mechanism of the interaction between gut microbiota and cocaine found that cocaine exposure increases intestinal norepinephrine levels, which promotes intestinal colonization and leads to a series of behavioral responses. Gut microbiota affects glycine levels, and through glutamatergic transmission, influences transcriptional plasticity in the nucleus accumbens, thereby modulating reward-related brain circuits associated with SUDs.
CELL HOST & MICROBE
(2022)
Review
Biochemistry & Molecular Biology
Ricarda Toerner, Tatsiana Kupreichyk, Wolfgang Hoyer, Jerome Boisbouvier
Summary: This article provides a comprehensive overview of the interaction between chaperones and amyloidogenic proteins, focusing on structural and mechanistic studies. It summarizes the advances made in preventing amyloid toxicity and highlights the challenges and remaining questions in this field.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Microbiology
Sebastian Urquiza-Zurich, Victor Antonio Garcia-Angulo, Paula Burdisso, M. Fernanda Palominos, Lucia Fernandez-Hubeid, Paloma A. Harcha, Juan P. Castillo, Andrea Calixto
Summary: By studying isolated bacteria found in association with wild nematodes in Chilean soil, we found that different bacterial assemblies can impact the life-history traits of the bacterivore nematode C. elegans, including behavioral and physiological outputs. We identified a new species of Stenotrophomonas and Bacillus pumilus as the isolated bacteria. These findings contribute to our understanding of the influence of microbiota on animal behavior choices.
Article
Biochemistry & Molecular Biology
Carlos A. Elena-Real, Annika Urbanek, Lionel Imbert, Anna Morato, Aurelie Fournet, Frederic Allemand, Nathalie Sibille, Jerome Boisbouvier, Pau Bernado
Summary: A strategy for efficiently assigning frequencies to individual nuclei in large biomolecular machines and repetitive proteins using in vitro protein expression is presented. The approach was demonstrated to be applicable for NMR assignment in four proteins by introducing isotopically labeled alanines. The labeling method enabled unambiguous assignments in large protein assemblies and revealed the correlation between helical stability and homorepeat length in Phox2B. Selectively introducing alanines with distinct labeling patterns proves to be a powerful tool for studying challenging biomolecular systems.
ACS CHEMICAL BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Matthieu P. M. H. Benoit, Byron Hunter, John S. Allingham, Hernando Sosa
Summary: This review discusses recent high-resolution structures of kinesins bound to microtubules or tubulin complexes, which have resolved outstanding questions about the basis of mechanochemical coupling and how modifications of the motor domain enable motility and/or microtubule depolymerization.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2023)
Article
Chemistry, Organic
Sebastian O. Simonetti, Teodoro S. Kaufman, Rodolfo M. Rasia, Ariel M. Sarotti, Nicolas Grimblat
Summary: By using a combination of DFT and chemometrics in NMR spectra analysis, researchers were able to identify the structure of the main decomposition products of hexamethylenetetramine. This approach also helped propose the structures of similar intermediates and unveil the structure of the main decomposition product of this particular structure.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2021)
Article
Virology
Marcel Chris Conrady, Irina Suarez, Gergo Gogl, Desiree Isabella Frecot, Anna Bonhoure, Camille Kostmann, Alexandra Cousido-Siah, Andre Mitschler, JiaWen Lim, Murielle Masson, Thomas Iftner, Frank Stubenrauch, Gilles Trave, Claudia Simon
Summary: The study compared the structures and efficiency of ternary complex formation between oncogenic E6 proteins of closely related high-risk HPV31 and HPV16 with their cellular targets p53 and E6AP. Despite high structural similarity, 31 E6 showed an overall lower binding affinity than 16 E6, aligning with the observed lower p53 degradation potential of 31 E6.
JOURNAL OF VIROLOGY
(2021)