期刊
NUCLEIC ACIDS RESEARCH
卷 43, 期 13, 页码 6181-6190出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkv582
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资金
- Engineering and Physical Sciences Research Council [EP/J019445/1]
- University College, Oxford
- EPSRC
- Engineering and Physical Sciences Research Council [EP/I001352/1, EP/J019445/1] Funding Source: researchfish
- EPSRC [EP/I001352/1, EP/J019445/1] Funding Source: UKRI
The effect of secondary structure on DNA duplex formation is poorly understood. Using oxDNA, a nucleotide level coarse-grained model of DNA, we study how hairpins influence the rate and reaction pathways of DNA hybridzation. We compare to experimental systems studied by Gao et al. (1) and find that 3-base pair hairpins reduce the hybridization rate by a factor of 2, and 4-base pair hairpins by a factor of 10, compared to DNA with limited secondary structure, which is in good agreement with experiments. By contrast, melting rates are accelerated by factors of similar to 100 and similar to 2000. This surprisingly large speed-up occurs because hairpins form during the melting process, and significantly lower the free energy barrier for dissociation. These results should assist experimentalists in designing sequences to be used in DNA nanotechnology, by putting limits on the suppression of hybridization reaction rates through the use of hairpins and offering the possibility of deliberately increasing dissociation rates by incorporating hairpins into single strands.
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