Review
Immunology
Yi Xing, Xun Sun, Yiming Dou, Min Wang, Yanmei Zhao, Qiang Yang, Yanhong Zhao
Summary: Extracellular vesicles (EVs) act as natural nanocarriers and intercellular messengers, playing a crucial role in cell communication, while macrophage-derived EVs may have significant implications in the development of chronic inflammatory diseases.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Ilan Pinkhasov, Liron Kabakov, Carlos E. E. Nemcovsky, Miron Weinreb, Pnina Schlesinger, Omer Bender, Maayan Gal, Daniel Z. Z. Bar, Evgeny Weinberg
Summary: The aim of this study was to investigate the heterogeneity of ex vivo-cultured human mesenchymal stromal cells derived from different regions of the oral mucosa. Single-cell RNA sequencing analysis revealed distinct cell sub-populations, with a higher proportion of mesenchymal stem cells in the masticatory mucosa. These findings have important implications for understanding cell function and potential therapeutic interventions.
JOURNAL OF CLINICAL PERIODONTOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xishuai Wang, Shiyu Zhao, Junhui Lai, Weijun Guan, Yang Gao
Summary: This study demonstrates that gingival-derived mesenchymal stem cells (GMSCs) intervene and protect against bleomycin-induced pulmonary fibrosis, while exhibiting anti-inflammatory and antioxidant capacities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Yalong Zhang, Zhiguo Wang, Bohong Shi, Yan Li, Ru Wang, Jiayao Sun, Yingzhe Hu, Changqing Yuan, Quanchen Xu
Summary: The study found that GMSC-Exos significantly reduced the release level of inflammatory factors and inhibited lipid accumulation in a high-lipid environment, while promoting the polarization of pro-inflammatory macrophages into an anti-inflammatory phenotype.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Anatomy & Morphology
Caiyun Ma, Yang Liu, Yingchun Ma, Lijie Jiang, Qianyi Huang, Gaofeng Liu, Yu Guo, Chunjing Wang, Changqing Liu
Summary: This study successfully isolated PMSCs from Sprague-Dawley rat fetal lung tissue for the first time and demonstrated their stemness and multi-differentiation potentials, suggesting a potential regenerative cell resource for cell regenerative therapy of lung injury.
Article
Cell Biology
Huey-Shan Hung, Wei-Chien Kao, Chiung-Chyi Shen, Kai-Bo Chang, Cheng-Ming Tang, Meng-Yin Yang, Yi-Chin Yang, Chun-An Yeh, Jia-Jhan Li, Hsien-Hsu Hsieh
Summary: The study created a nanocomposite of PEG with various concentrations of Au nanoparticles, with PEG-Au 43.5 ppm showing superior biocompatibility and biological properties for MSCs, especially in neuronal differentiation. This nanocomposite enhanced cell adhesion, proliferation, and migration in MSCs, prevented apoptosis, inhibited inflammation, and promoted differentiation into various cell types, including nerve cells.
Review
Biochemistry & Molecular Biology
Erika B. Danella, Marcell Costa de Medeiros, Nisha J. D'Silva
Summary: The oral mucosa plays a crucial role in protecting against physical, microbial, and chemical damage. When compromised, the wound healing response is activated, which involves processes such as immune infiltration, re-epithelialization, and stroma remodeling, all controlled by cytokines that promote cellular migration, invasion, and proliferation. These same cytokines are also implicated in cancer dissemination. Therefore, studying the cytokines involved in oral wound healing can provide insights into the cytokines exploited by oral squamous cell carcinoma (SCC) to promote tumor development and progression.
Article
Chemistry, Multidisciplinary
Jiayao Sun, Zhiguo Wang, Peng Liu, Yingzhe Hu, Tingting Li, Jianbo Yang, Pengyu Gao, Quanchen Xu
Summary: This study aimed to explore the effects of exosomes derived from human gingival mesenchymal stem cells (GMSC-Exo) on the inflammatory response of periodontal ligament stem cells (PDLSCs) in an inflammatory microenvironment. The study found that GMSC-Exo attenuated the inflammatory response of PDLSCs by regulating the expression of NF-kappa B signaling and Wnt5a.
FRONTIERS IN CHEMISTRY
(2022)
Article
Engineering, Biomedical
Norman Michael Drzeniek, Nourhan Kahwaji, Stephan Schlickeiser, Petra Reinke, Sven Geissler, Hans-Dieter Volk, Manfred Gossen
Summary: We improved the immunogenicity of in vitro transcribed mRNA through chemical modification of nucleotides, and successfully modulated the secretion phenotype of therapeutic cells with mRNA encoding vascular endothelial growth factor (VEGF). Cell encapsulation in a collagen-hyaluronic acid hydrogel increased the secretion of VEGF encoded by mRNA, as well as the endogenous secretion of supporting pro-angiogenic mediators such as hepatocyte growth factor (HGF).
Article
Biochemistry & Molecular Biology
Nerea Sanchez, Fabio Vignoletti, Ignacio Sanz-Martin, Alejandro Coca, Javier Nunez, Estela Maldonado, Javier Sanz-Esporrin, Irene Hernando-Pradies, Silvia Santamaria, David Herrera, Jose A. Garcia-Sanz, Mariano Sanz
Summary: The combination of xenogeneic collagen matrix and autologous GMSCs with a coronally advanced flap showed promising results in reducing inflammation, promoting new cementum/bone formation, and reducing gingival recession compared to other treatment groups, although the differences were not statistically significant.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Anja E. Luengen, Maria Cheremkhina, Julian Gonzalez-Rubio, Jan Weckauf, Caroline Kniebs, Hendrik Uebner, E. Miriam Buhl, Christian Taube, Christian G. Cornelissen, Thomas Schmitz-Rode, Stefan Jockenhoevel, Anja Lena Thiebes
Summary: The study shows that tri-cultures supported by BM-MSC and HNF are most similar to native respiratory epithelium in terms of epithelial morphology, ciliation, and mucin production. They also promote mucociliary differentiation and vascularization. Ciliation is reduced in tri-cultures compared to co-cultures, while vascular networks are more branched and extended. Cytokine concentrations in tri-cultures are lower than in co-cultures.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Immunology
Giulia Franzoni, Samanta Mecocci, Chiara Grazia De Ciucis, Lorena Mura, Filippo Dell'Anno, Susanna Zinellu, Floriana Fruscione, Livia De Paolis, Tania Carta, Antonio G. Anfossi, Silvia Dei Guidici, Elisabetta Chiaradia, Luisa Pascucci, Annalisa Oggiano, Katia Cappelli, Elisabetta Razzuoli
Summary: This study investigated the immunomodulatory effects of goat milk extracellular vesicles (EVs) on untreated and classically activated porcine monocyte-derived macrophages. The results showed that these particles were efficiently internalized by macrophages and high doses upregulated MHC I and MHC II DR on untreated macrophages, while low doses enhanced the gene expression of IL10, EBI3, and IFNB. High doses also upregulated several pro-inflammatory cytokines. Overall, these findings support the clinical application of these molecules in polarizing macrophages towards an M1-like phenotype.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell & Tissue Engineering
Yuxin Zhao, Bobo Cai, Weijun Zhu, Jue Shi, Yu Wang, Misi Si
Summary: The study investigated the therapeutic effect of IL-1ra on GMSCs under an inflammatory microenvironment induced by P. gingivalis-LPS. Results demonstrated that IL-1ra effectively decreased inflammatory cytokine production, protecting GMSCs' osteogenesis capacity and other biological properties.
STEM CELLS INTERNATIONAL
(2021)
Article
Pharmacology & Pharmacy
Solen Novello, Sylvie Tricot-Doleux, Agnes Novella, Pascal Pellen-Mussi, Sylvie Jeanne
Summary: This study evaluated the influence of periodontal ligament stem cell-derived conditioned medium (CM) on osteoblasts and its potential as a therapeutic tool for periodontal regeneration. The findings showed that PDLSC-CM enhanced osteoblast proliferation and increased the expression of some osteoblastic differentiation markers.
Article
Dentistry, Oral Surgery & Medicine
Koya Nakamura, Toshiro Yamamoto, Ryo Ema, Kei Nakai, Yoshiki Sato, Kenta Yamamoto, Keiji Adachi, Fumishige Oseko, Yoshiaki Yamamoto, Narisato Kanamura
Summary: The study found that mechanical stress applied through dentures increases the production of inflammatory cytokines from oral mucosal epithelial cells and fibroblasts, likely through the p38 MAPK cascade, leading to inflammation. On the other hand, up-regulation of growth factors may be a biological defense mechanism against excessive mechanical stress.