Article
Biochemistry & Molecular Biology
Manuel Mata, Santiago Peydro, Jose Javier Martin de Llano, Maria Sancho-Tello, Carmen Carda
Summary: Periodontitis is an inflammatory disease that can lead to tooth loss. This study examined the role of surface microtopography in the differentiation of human dental pulp stem cells into cementoid-like secreting cells. The results showed that the cells cultured on dentin surfaces exhibited an odontoblastic phenotype, while cells cultured on cementum surfaces generated a well-organized tissue consisting of secretory cells and dense fibrous connective tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Dentistry, Oral Surgery & Medicine
Minyan Yuan, Xueqing Zheng, Yifan Xue, Zhenru He, Guangtai Song, Yaling Song
Summary: The study aims to investigate a Chinese family with dentin dysplasia type II (DD-II) and summarize mutation hotspots, clinical manifestations, and disease management strategies. A novel pathogenic mutation (c.2035delA) in the DPP region of DSPP was identified, which was cosegregated in the family. The study revealed new clinical features of DD-II and identified a mutation hotspot in the DSPP gene.
Article
Cell Biology
Hye Jung Ihn, Ju Ang Kim, Jiwon Lim, Sang-Hyeon Nam, So Hyeon Hwang, Young Kyung Kim, Jae-Young Kim, Jung-Eun Kim, Eui-Sic Cho, Rulang Jiang, Eui Kyun Park
Summary: Tooth root development involves the interaction of growth factors and transcription factors in Hertwig's epithelial root sheath and dental mesenchyme. Knockout of the Bbx gene in mice results in reduced length of tooth roots and downregulation of Dspp expression during early root formation, suggesting a role for Bbx in tooth root formation.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Isidoro Cobo, Sumit Paliwal, Cristina Bodas, Irene Felipe, Julia Melia-Aloma, Ariadna Torres, Jaime Martinez-Villarreal, Marina Malumbres, Fernando Garcia, Irene Millan, Natalia del Pozo, Joo-Cheol Park, Ray J. MacDonald, Javier Munoz, Raul Mendez, Francisco X. Real
Summary: NFIC is a transcription factor that regulates the function of pancreatic acinar cells and suppresses the development of pancreatic ductal adenocarcinoma. It controls the differentiation and physiological functions of pancreatic acinar cells by regulating the expression of acinar genes, including those involved in RNA and protein metabolism, as well as the genes involved in resolving endoplasmic reticulum stress. In pancreatic cancer, NFIC expression is down-regulated, leading to the formation of pre-neoplastic lesions driven by mutant KRAS.
NATURE COMMUNICATIONS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Zhenwei Zhang, Guibin Huang, Yu Huang, Siyi Liu, Feng Chen, Xuejun Gao, Yanmei Dong, Hua Tian
Summary: This study aimed to identify candidate genes for inheritable dentin defects in three Chinese pedigrees and characterize the property of affected teeth. Clinical and radiological features were recorded for the affected individuals. Genomic analysis identified three novel frameshift mutations of dentin sialophosphoprotein gene related to inherited dentin defects.
ARCHIVES OF ORAL BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Mahmoud Mona, Zunnaira Abbasi, Firas Kobeissy, Abdulrahman Chahbandar, Roberta Pileggi
Summary: External root resorption (ERR) is a silent destructive phenomenon detrimental to dental health, which may have multiple etiologies. Traditional diagnostic methods like radiographs and CBCT images are not precise enough, highlighting the need for more accurate and non-invasive diagnostic approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Dandrich Lim, Ko-Chien Wu, Arthur Lee, Thomas L. Saunders, Helena H. Ritchie
Summary: Dentin Sialoprotein (DSP) and phosphophoryn (PP) are two major non-collagenous proteins in dentin that play essential roles in dentin formation. Using transgenic technology, the DSPP gene knockout defect in mice can be partially or completely rescued, indicating the importance of DSPP in tooth development.
Article
Endocrinology & Metabolism
Rui Sheng, Yuan Wang, Yunshu Wu, Jun Wang, Shiwen Zhang, Qiwen Li, Danting Zhang, Xingying Qi, Qingyue Xiao, Shuang Jiang, Quan Yuan
Summary: This study reveals the crucial role of m(6)A methyltransferase METTL3 in tooth root development by influencing NFIC translation efficiency, shedding light on a novel epigenetic mechanism in tooth root formation.
JOURNAL OF BONE AND MINERAL RESEARCH
(2021)
Article
Physiology
Tian Liang, Qian Xu, Hua Zhang, Suzhen Wang, Thomas G. H. Diekwisch, Chunlin Qin, Yongbo Lu
Summary: The mutant P19L-DSPP protein caused developmental enamel defects in mice, potentially linked to intracellular retention of mutant DSPP in presecretory ameloblasts.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Muhammad Chair Effendi, Ahmad Taufiq, Boy Muchlis Bachtiar, Endang Winiati Bachtiar, Ellyza Herda
Summary: The study revealed that DPSCs exposed to NMT showed enhanced proliferation and differentiation, demonstrating dentin formation and extracellular matrix formation.
Article
Engineering, Biomedical
Luyi Zhao, Jian Sun, Ce Zhang, Chaoqun Chen, Yi Chen, Bo Zheng, Haihua Pan, Changyu Shao, Biao Jin, Ruikang Tang, Xinhua Gu
Summary: By investigating the biomimetic remineralization of demineralized dentin with Asp, it was found that Asp can promote the crystallization kinetics of PAA-stabilized ACP to HAP, shorten the remineralization time, and result in remineralized dentin with similar morphology and biological stability to natural teeth. This experiment reveals the mechanism of action of Asp in dentin biomineralization and demonstrates the potential for biomimetic remineralization of dentin with good biological properties.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2021)
Article
Cell Biology
Fengxia Chen, Qingqing Wang, Xiaoyan Yu, Ningning Yang, Yuan Wang, Yangyang Zeng, Zhewen Zheng, Fuxiang Zhou, Yunfeng Zhou
Summary: TNBC is a highly aggressive subtype with poor prognosis and high metastatic potential. Downregulation of MCPIP1 in TNBC is associated with poor survival rates, while its overexpression can suppress cell proliferation by regulating alternative splicing. MCPIP1's role as a tumor suppressor and potential treatment target in TNBC is highlighted in this study.
CELL DEATH & DISEASE
(2021)
Article
Dentistry, Oral Surgery & Medicine
Vellore Kannan Gopinath, Sheela Soumya, V. Yogeshwar Chakrapani, T. S. Sampath Kumar
Summary: This study developed electrospun nanofibrous membranes blending PCL with HA, with PCL10%HA membrane showing the most favorable effects on cell attachment, upregulation of gene expression, and Ca/P ratio compared to PCL and PCL15%HA membranes in inducing differentiation of IDPSCs into odontoblasts.
DENTAL MATERIALS JOURNAL
(2021)
Article
Health Care Sciences & Services
Youn Jung Kim, Yejin Lee, Hong Zhang, Figen Seymen, Mine Koruyucu, Sule Bayrak, Nuray Tuloglu, James P. Simmer, Jan C-C Hu, Jung-Wook Kim
Summary: This study identified three novel mutations associated with hereditary dentin defects and expanded the mutational spectrum of the DSPP gene. The study also found that the expression level of the DSPP exon 3 deletion transcript correlated with the severity of dentin defects, advancing our understanding of the molecular pathogenesis of this condition.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Cell Biology
Kunihiro Kajiura, Naoki Umemura, Emika Ohkoshi, Takahisa Ohta, Nobuo Kondoh, Satoshi Kawano
Summary: The study demonstrates that shikonin can induce odontoblastic differentiation of dental pulp stem cells through the involvement of the AKT-mTOR signaling pathway and CD44. While CD44 expression is crucial for odontoblastic differentiation of dental pulp stem cells, the relationship between shikonin-induced expression of DSPP and the AKT-mTOR signaling pathway and CD44 expression remains to be fully elucidated.
CONNECTIVE TISSUE RESEARCH
(2021)
Article
Dentistry, Oral Surgery & Medicine
Maria Akhtar, Kyung Mi Woo, Muhammad Tahir, Wenhui Wu, Jeevithan Elango, Munazza R. Mirza, Maryam Khan, Saba Shamim, Praveen R. Arany, Saeed Ur Rahman
Summary: The study demonstrated that LiCl-incorporated nanofiber scaffolds can enhance osteoblast differentiation by promoting cell proliferation and increasing expression of proteins associated with osteogenesis.
CLINICAL ORAL INVESTIGATIONS
(2022)
Article
Cell Biology
Hye-Rim Shin, Bong-Soo Kim, Hyun-Jung Kim, Heein Yoon, Woo-Jin Kim, Je-Yong Choi, Hyun-Mo Ryoo
Summary: This study finds that the main cause of limb shortening in Apert syndrome resulting from FGFR2 gene mutations is the excessive expression of RANKL, which leads to an imbalance in osteoblast and osteoclast coupling.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Kyeong-Rok Kang, Jae-Sung Kim, Jeong-Yeon Seo, Hyang Lim, Tae-Hyeon Kim, Sun-Kyoung Yu, Heung-Joong Kim, Chun Sung Kim, Hong Sung Chun, Joo-Cheol Park, Do Kyung Kim
Summary: The present study investigates the role of NAMPT in odontogenic differentiation during tooth development in mice. The up-regulation of NAMPT promotes odontogenic differentiation and mineralization, while the inhibition of NAMPT attenuates odontogenic differentiation.
KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Hoon Seonwoo, Han-Wool Choung, Sangbae Park, Kyoung Soon Choi, Kyoung-Je Jang, Jangho Kim, Ki-Taek Lim, Yeonju Kim, Pankaj Garg, Shambhavi Pandey, Juo Lee, Joo-Cheol Park, Yun-Hoon Choung, Pill-Hoon Choung, Soo Young Kim, Jong Hoon Chung
Summary: Natural calcium phosphate cements (CPCs) derived from sintered animal bone have low mechanical strength, but incorporating reduced graphene oxide (RGO) and applying pulsed electromagnetic fields (PEMFs) can significantly improve their mechanical properties and osteogenic differentiation ability. The chemical bonding between RGO and CPCs enhances the mechanical properties, while PEMFs enhance osteogenic differentiation of stem cells. RGO-CPCs with PEMFs show improved biocompatibility and osteoinductivity in both in vitro and in vivo studies.
Article
Biochemistry & Molecular Biology
Hyun-Jung Kim, Woo-Jin Kim, Hye-Rim Shin, Hee-In Yoon, Jae- Moon, Eunji Lee, Jin-Muk Lim, Young-Dan Cho, Mi-Hye Lee, Hong-Gee Kim, Hyun-Mo Ryoo
Summary: Cellular senescence is closely related to tissue aging, including bone. Oxidative stress caused by the accumulation of reactive oxygen species (ROS) accelerates cellular senescence and dysfunction of osteoblasts. This study identified PADI2 as a regulator of ROS-accelerated senescence of osteoblasts and provided new insights into excessive ROS-promoted cellular senescence and aging-related bone diseases.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Dentistry, Oral Surgery & Medicine
Shengfeng Bai, Ji-Hyun Lee, Chul Son, Dong-Seol Lee, Joo-Cheol Park
Summary: The study demonstrates that CPNE7 plays a regulatory role in periodontal ligament (PDL) repair by supporting PDL alignment through TAU-mediated cytoskeleton reorganization and direct regulation of CAP-mediated PDL attachments of PDLCs.
JOURNAL OF CLINICAL PERIODONTOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Yoon Seon Lee, Yeoung-Hyun Park, You-Mi Seo, Hye-Kyung Lee, Joo-Cheol Park
Summary: This study identified that the Smad4-dependent TGF-beta 1/BMP2 signaling pathway regulates the expression of Cpne7 in dental epithelial cells, and the CPNE7 protein mediates odontoblast differentiation through epithelial-mesenchymal interaction. The results showed that conditioned medium treated with TGF-beta 1/BMP2 can induce the formation of tubular dentin.
Article
Dentistry, Oral Surgery & Medicine
Hye Ri Gug, Yeoung-Hyun Park, Su-Jin Park, Ji Yeon Jang, Ji-Hyun Lee, Dong-Seol Lee, Won -Jun Shon, Joo-Cheol Park
Summary: This study evaluated the potential application of CPNE7-derived functional peptide (CPNE7-DP) in dentin caries. The results showed that CPNE7-DP was stable in caries-like environments and regenerated tubular tertiary dentin, alleviating inflammation and reducing the expression of inflammatory cytokines. CPNE7-DP may help overcome the limitations of current restorative caries treatments.
ARCHIVES OF ORAL BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Dong-Seol Lee, Yeo Joon Song, Hye Ri Gug, Ji-Hyun Lee, Hyun Sook Bae, Joo-Cheol Park
Summary: Tooth development involves cell divisions and interactions, and the NFI-C transcription factor plays a crucial role. This study reveals that Nfic regulates cell proliferation in various mineralized tissue stem cells, and is involved in the expression of pluripotency genes. Especially in dental epithelial stem cells, Nfic regulates epithelial cell proliferation through epithelial-mesenchymal interactions.
STEM CELLS INTERNATIONAL
(2022)
Article
Engineering, Biomedical
Saeed Ur Rahman, Woo-Jin Kim, Shin Hye Chung, Kyung Mi Woo
Summary: This study demonstrates that the fibrous topography of scaffolds can direct the differentiation of odontoblast-lineage cells to have the three-dimensional structure of odontoblasts. The altered responsiveness to Wnt5a on the nanofibrous substrate mediated the phosphorylation of JNK and polarity growth signaling, leading to the regeneration of tubular dentin with odontoblast processes. These findings suggest the potential application of nanofibrous scaffolds for dentin regeneration and provide evidence for the distinctive cell responsiveness to their micro-environments.
MATERIALS TODAY BIO
(2022)
Article
Dentistry, Oral Surgery & Medicine
Hyang Lim, Ji-Su Oh, Kyeong-Rok Kang, Jeong-Yeon Seo, Do Kyung Kim, Sun-Kyoung Yu, Heung-Joong Kim, Joo-Cheol Park, Jae-Sung Kim
Summary: This study found that 25-HC can induce odontoclastogenesis in MDPC-23 cells by modulating the RANK-RANKL-OPG axis, potentially contributing to the pathophysiological processes of internal tooth resorption.
INTERNATIONAL ENDODONTIC JOURNAL
(2023)
Article
Dentistry, Oral Surgery & Medicine
Juhyun Lee, Geumbit Hwang, Hyeri Gug, Ji-Hyun Lee, Su-Jin Park, Joo-Cheol Park
Summary: This study investigated the effects of toothpastes containing CPNE7-derived oligopeptide (CPNE7-DP) and other active desensitizing ingredients on dentin microleakage, tubule occlusion, and tertiary dentin formation. The results showed that toothpaste with CPNE7-DP had a long-term dentin sealing effect and promoted the formation of new hard tissue.
Article
Biochemistry & Molecular Biology
Jae- Moon, Woo-Jin Kim, Ki-Tae Kim, Hyun-Jung Kim, Hye-Rim Shin, Heein Yoon, Seung Gwa Park, Min-Sang Park, Young-Dan Cho, Pil-Jong Kim, Hyun-Mo Ryoo
Summary: An innovative automated image analysis method is presented in this study for precise quantification of subcellular foci within the cell nucleus. The method, called Foci-Xpress, is significantly faster and equally accurate compared to other conventional methods. Its automation eliminates biases caused by researchers and enhances experimental efficiency.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Heein Yoon, Seung Gwa Park, Hyun-Jung Kim, Hye-Rim Shin, Ki-Tae Kim, Young-Dan Cho, Jae- Moon, Min-Sang Park, Woo-Jin Kim, Hyun-Mo Ryoo
Summary: A study found that a form of vitamin B3 called nicotinamide (NAM) can prevent or treat bone diseases related to oxidative stress by increasing the production of antioxidant enzymes. NAM enhances mitochondrial function, increases the production of antioxidant enzymes, and promotes the formation of bone-building cells called osteoblasts. NAM increases mitochondrial biogenesis and antioxidant enzyme expression through activation of the SIRT3-FOXO3A axis, enhancing osteoblast differentiation.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2023)
Article
Multidisciplinary Sciences
Geumbit Hwang, Hyejin Seo, Joo-Cheol Park
Summary: Cpne7 deficiency leads to hepatic lipid metabolism abnormalities, excessive ROS production, and mitochondrial dysfunction. These findings are important for understanding the pathogenesis of NAFLD and suggest Cpne7 as a potential therapeutic target for NAFLD.