期刊
JOURNAL OF DENTAL RESEARCH
卷 87, 期 10, 页码 932-936出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/154405910808701010
关键词
cathepsins; cystatins; Porphyromonas gingivalis; periodontitis
资金
- INSERM, the Ministry of Education and Research
- Universite Louis Pasteur of Strasbourg [ERT 1061]
Porphyromonas gingivalis is a major etiological agent of periodontitis that could affect the expression of Cathepsins B and C by disrupting the balance between these enzymes and their inhibitor, Cystatin C. We tested this hypothesis by infecting human oral epithelial cells with P. gingivalis or activating solely by its lipopolysaccharide. The mRNA level, the enzymatic activity, and the protein expression of Cathepsin B were increased (three-fold) in a dose-dependent manner, while those of Cystatin C decreased (five-fold). No changes were observed for Cathepsin C. Although activation by lipopolysaccharides led to a delayed imbalance (2 days) between Cathepsin B and Cystatin C, this imbalance took place very rapidly during the infection (< 6 hrs), indicating that the whole bacterium contains components that initiate rapid changes in the transcription rates of Cathepsin B and Cystatin C and selectively modify the molecular pathways that lead to this imbalance.
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