Review
Biochemistry & Molecular Biology
Spela Gruden, Natasa Poklar Ulrih
Summary: Lactoferrins and their derivatives have important protective roles in the body, including antimicrobial, antitumor, anti-inflammatory, immunomodulatory, and antioxidant activities. While their antimicrobial activity has been extensively studied, the specific mechanisms behind their actions are still not fully understood.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
Ye Wang, James D. Morton, Alaa EL-Din A. Bekhit, Alan Carne, Susan L. Mason
Summary: Deer lactoferrin shares similarities with bovine lactoferrin in terms of amino acid sequence, but exhibits some differences in antimicrobial activity when compared with lactoferrin peptides.
Article
Biochemistry & Molecular Biology
Aysel Aslanli, Maksim Domnin, Nikolay Stepanov, Elena Efremenko
Summary: The combined use of various antimicrobial peptides (AMPs) with enzymes that hydrolyze quorum sensing molecules is a leading approach in solving antimicrobial resistance. In this study, lactoferrin-derived AMPs were investigated as potential partners for combination with enzymes hydrolyzing lactone-containing QS molecules. The His(6)-OPH/Lfcin combination showed the most promising results in terms of stability and catalytic efficiency, demonstrating its potential as an effective antimicrobial agent.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Catia Santos-Pereira, Juliana F. Rocha, Henrique S. Fernandes, Ligia R. Rodrigues, Manuela Corte-Real, Sergio F. Sousa
Summary: Lactoferrin is a bioactive milk protein with strong anticancer and antifungal activities, and its interaction with the proton pump V-ATPase leads to cell death. The study employed computational methods to elucidate the binding mechanism of lactoferrin to V-ATPase, revealing that lactoferrin inhibits ATP hydrolysis by binding to the cytosolic domain of V-ATPase.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Yifan Liang, Shin-ichi Ikeda, Junhan Chen, Yan Zhang, Kazuno Negishi, Kazuo Tsubota, Toshihide Kurihara
Summary: Myopia is a leading cause of vision impairment, and an effective intervention is needed. This study examined the effects of different forms of lactoferrin (LF) on myopia in mice and found that digested LF and holo-LF were more effective in suppressing myopia compared to native LF. The results also revealed that mice given digested LF or holo-LF had a less elongated axial length and thinner choroid, as well as lower levels of certain cytokines and growth factors associated with myopia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Infectious Diseases
Katherine Aguirre-Guataqui, Mateo Marquez-Torres, Hector Manuel Pineda-Castaneda, Yerly Vargas-Casanova, Andres Ceballos-Garzon, Zuly Jenny Rivera-Monroy, Javier Eduardo Garcia-Castaneda, Claudia Marcela Parra-Giraldo
Summary: Antimicrobial peptides are valuable resources for the discovery or design of potential antifungal candidates. Bovine lactoferricin and Buforin II are AMPs with great antimicrobial potential. Chimeras containing the minimal active motifs of these peptides were synthesized and evaluated for their antifungal activity against various strains of Candida. Some chimeras exhibited strong antifungal activity against specific strains.
Article
Immunology
Jacky Lu, Miriam A. Guevara, Jamisha D. Francis, Sabrina K. Spicer, Rebecca E. Moore, Schuyler A. Chambers, Kelly M. Craft, Shannon D. Manning, Steven D. Townsend, Jennifer A. Gaddy
Summary: This study demonstrates that lactoferrin possesses antimicrobial and antibiofilm properties against a wide range of GBS isolates, with maternally isolated colonizing strains being the most susceptible.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Review
Pharmacology & Pharmacy
Anna Ohradanova-Repic, Romana Prazenicova, Laura Gebetsberger, Tetiana Moskalets, Rostislav Skrabana, Ondrej Cehlar, Gabor Tajti, Hannes Stockinger, Vladimir Leksa
Summary: Lactoferrin is an iron-binding glycoprotein that is found in human exocrine fluids, especially breast milk. It has diverse roles in host defense, including modulating immune cell functions, sequestering iron, and binding to various surfaces. Lactoferrin can be cleaved to generate lactoferricin, which also has bioactive properties. This review discusses the structure, functions, and potential therapeutic uses of lactoferrin, lactoferricin, and other lactoferrin-derived peptides in treating infections and inflammatory conditions, with a focus on their potential use in treating COVID-19.
Article
Chemistry, Applied
Elena Biasibetti, Silvia Rapacioli, Natascia Bruni, Elisa Martello
Summary: Antibiotic resistance is a global problem, and the search for new antimicrobial agents is urgent. This study explored the antibacterial and antimicrobial effects of milk-derived antimicrobial agents, lactoferrin-derived peptides, lactoferricin (Lfc) and lactoferrampin (Lfa), either alone or in combination, in vitro against various pathogens. The results showed that natural-derived compounds, such as milk peptides and plant extracts, could be promising tools for treating moderate fungal and bacterial infections.
NATURAL PRODUCT RESEARCH
(2021)
Review
Food Science & Technology
Jiajia Wu, Mingwu Zang, Shouwei Wang, Xiaoling Qiao, Bing Zhao, Jing Bai, Yan Zhao, Yuxuan Shi
Summary: The growth of bacteria and fungi may cause diseases in humans and spoilage of food. Lactoferricin (LFcin) is a group of antimicrobial peptides derived from milk protein lactoferrin (LF). LFcin has better antimicrobial ability than its parent version and can be used in food preservation. This study identified 43 new LFcin peptides and expands the LFcin family, which is useful for further research on peptides with antimicrobial potential.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
Jacky Lu, Jamisha D. Francis, Miriam A. Guevara, Rebecca E. Moore, Schuyler A. Chambers, Ryan S. Doster, Alison J. Eastman, Lisa M. Rogers, Kristen N. Noble, Shannon D. Manning, Steven M. Damo, David M. Aronoff, Steven D. Townsend, Jennifer A. Gaddy
Summary: Group B Streptococcus (GBS) is a pathogen that causes invasive infections in pregnant hosts, neonates, and immunocompromised individuals, while human breast milk lactoferrin has antimicrobial and anti-biofilm activity against GBS.
Article
Biochemistry & Molecular Biology
Gopal Ramamourthy, Hans J. Vogel
Summary: Pathogenic bacteria can form biofilms to resist antibiotics, but synthetic dimeric peptides derived from lactoferrin show potential in preventing and disrupting biofilms of Pseudomonas aeruginosa, providing a new direction for antibiofilm agent development.
BIOCHEMISTRY AND CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Michal Ostrowka, Anna Duda-Madej, Filip Pietluch, Pawel Mackiewicz, Przemyslaw Gagat
Summary: Lactoferrin, an iron-binding glycoprotein, has multiple functions including antimicrobial, antiviral, antifungal, anticancer, antioxidant, and immunomodulatory activities. This study generated three novel human lactoferrin fragments, which were predicted to have antimicrobial activity. However, none of the new fragments showed antimicrobial properties, except for hLF 1-11, which was lethal against Pseudomonas aeruginosa.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Infectious Diseases
Silvia Katherine Carvajal, Yerly Vargas-Casanova, Hector Manuel Pineda-Castaneda, Javier Eduardo Garcia-Castaneda, Zuly Jenny Rivera-Monroy, Claudia Marcela Parra-Giraldo
Summary: This study evaluated the binding and antifungal activity of chimeras composed of bovine lactoferricin (LfcinB) and buforin II (BFII) against Cryptococcus neoformans var. grubii. The chimeras showed high activity against the strains, with a synergistic effect when combined with fluconazole treatment.
Article
Biochemistry & Molecular Biology
Liang Wang, Yu-Lian Wang, Zi-Li Lv, En-peng Zhang, Ai-zhen Guo
Summary: By optimizing the design of bovine lactoferricin-derived peptide and constructing a recombinant expression vector, a stronger antibacterial activity against Staphylococcus aureus was obtained compared to natural bovine lactoferrin peptide under the same fermentation conditions. The effective expression of the optimized peptide was confirmed using SDS-PAGE electrophoresis, laying the foundation for improving the biological activities of antimicrobial peptides.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)