Article
Veterinary Sciences
Yu Jin Lee, Young Ju Lee
Summary: This study investigated the pathogenic potential of Coagulase-negative staphylococci (CoNS) isolated from bulk tank milk. The results showed that these bacteria exhibited a high prevalence of biofilm-forming ability and multidrug resistance. Therefore, developing a strong monitoring and sanitation program for dairy factories is crucial for ensuring hygienic milk production.
VETERINARY SCIENCES
(2022)
Article
Microbiology
Vanessa Silva, Elisete Correia, Jose Eduardo Pereira, Camino Gonzalez-Machado, Rosa Capita, Carlos Alonso-Calleja, Gilberto Igrejas, Patricia Poeta
Summary: This study evaluates the ability of biofilm formation in coagulase-negative staphylococci (CoNS) and S. pseudintermedius isolated from various animal species, and investigates the effect of conventional antimicrobials on reducing biofilm. The results show that all isolates can form biofilm, with S. urealyticus producing the most biomass and S. pseudintermedius producing the least biomass. Biofilm formation is positively associated with multidrug resistance and resistance to individual antimicrobials. Tetracycline and amikacin were unable to eradicate the biofilm, even at high concentrations.
Article
Chemistry, Applied
Li Yang, Hongjun Li, Han Wu, Shuyun Liu, Chang Su, Zhifei He
Summary: This study isolated and identified Coagulase-negative Staphylococci (CNS) from Chinese bacon, and evaluated their ability to degrade proteins and improve the taste of the bacon. Two CNS species were found to be capable of degrading proteins and enhancing the taste of Chinese bacon.
Review
Microbiology
Angela Franca, Vania Gaio, Nathalie Lopes, Luis D. R. Melo
Summary: Coagulase-negative staphylococci (CoNS) have become major pathogens in healthcare-associated facilities, with a focus on biofilm formation and antibiotic resistance genes. These factors make treatment challenging, despite their lower virulence compared to S. aureus.
Article
Food Science & Technology
Wioleta Chajecka-Wierzchowska, Joanna Gajewska, Anna Zadernowska, Cinzia Lucia Randazzo, Cinzia Caggia
Summary: The present study aimed to evaluate and describe the diversity of CoNS strains as potential vectors for the transmission of antimicrobial resistance from RTE foods served in bars and restaurants. The study found that out of 85 CoNS strains, 67 strains were resistant to at least one antibiotic, with 37 strains exhibiting multidrug resistance (MDR-CoNS). Furthermore, CoNS strains carried resistance genes for antibiotics critically important in medicine, such as beta-lactams, aminoglycosides, and tetracyclines. The high frequency of MDR-CoNS in RTE foods poses a direct risk to public health as it contributes to the gene pool for acquisition of resistance traits by pathogenic bacteria.
Article
Microbiology
Vladimir Gostev, Semen Leyn, Alexander Kruglov, Daria Likholetova, Olga Kalinogorskaya, Marina Baykina, Natalia Dmitrieva, Zlata Grigorievskaya, Tatiana Priputnevich, Lyudmila Lyubasovskaya, Alexey Gordeev, Sergey Sidorenko
Summary: Coagulase-negative staphylococci (CoNS) were historically considered non-pathogenic, but strains such as S. epidermidis, S. haemolyticus, and S. hominis have now been identified as opportunistic pathogens. The off-label use of linezolid for CoNS infections has led to the emergence of international linezolid-resistant lineages. Genetic analysis revealed the presence of common ancestors for linezolid-resistant strains in different countries.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Luiza Pinheiro-Hubinger, Danilo Flavio Moraes Riboli, Ligia Maria Abraao, Eliane Patricia Lino Pereira Franchi, Maria de Lourdes Ribeiro de Souza da Cunha
Summary: This study compared the clonality and SCCmec type of CoNS isolates from different sources in the same community, revealing high clonal diversity in S. epidermidis and circulation of some S. haemolyticus clones in both hospital and community. The presence of different SCCmec types within the same cluster indicated high diversity, suggesting SCCmec may not be a good marker of clonality in these bacteria.
Article
Infectious Diseases
Nisa Sipahi, Ertugrul Kaya, Cansu Celik, Orhan Pinar
Summary: This study aimed to identify staphylococcal species in raw milk and determine their hemolysis, biofilm, coagulase activities, and β-lactam resistance. The results showed that these staphylococcal species exhibited certain virulence factors and antibiotic resistance genes, highlighting the need for continuous monitoring and attention to resistance concerns.
Article
Infectious Diseases
Anyaphat Srithanasuwan, Montira Intanon, Wasana Chaisri, Witaya Suriyasathaporn
Summary: The study aimed to determine the ability of coagulase-negative staphylococci (CNS) to survive in the same environment as major pathogens such as Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli. Results showed differences in the survival percentages among CNS species in all conditions. While most CNS strains could survive when cultured prior to major strains, some strains were able to degrade Streptococcus agalactiae. Although most CNS strains were degraded in the after and equal conditions, certain strains could resist Staphylococcus aureus and Streptococcus agalactiae. Research on these survival strains may suggest the possibility of novel bacteria with bacteriolytic capabilities as a new mastitis treatment.
Article
Infectious Diseases
Christine M. Mbindyo, George C. Gitao, Paul Joseph Plummer, Benard W. Kulohoma, Charles M. Mulei, Rawlynce Bett
Summary: The study revealed a high prevalence of multidrug-resistant staphylococci strains associated with mastitis in dairy cows in Kenya, posing a threat to both animal and human health. Different resistance genes and multidrug resistance were observed in both Staphylococcus aureus and coagulase-negative staphylococci species.
Article
Agriculture, Dairy & Animal Science
A. Schnitt, T. Lienen, H. Wichmann-Schauer, B-A Tenhagen
Summary: This study found the occurrence of MR-NAS on dairy farms in Germany, with varying positive rates among different types of samples and in the environment. Nine species of MR-NAS were identified. Similar mobile genetic elements were detected in MR-NAS and MRSA on some farms, suggesting potential transfer of resistance genes between NAS and Staph. aureus on these farms.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Infectious Diseases
Yahya Mohzari, Fahad Aljobair, Ahmed Alrashed, Syed Mohammed Basheeruddin Asdaq, Renad Abdullah Alshuraim, Suzan Suhail Asfour, Mountasser Mohammad Al-Mouqdad, Reem F. Bamogaddam, Deemah Al-Anazi, Catherine E. Zeilinger, Ahmad Alamer, Batool Mohammed Alhassan, Nagaraja Sreeharsha
Summary: In recent years, there has been an increase in the prevalence of gram-positive bacteremia in neonates, but better care has also led to improved survival rates for premature neonates. Coagulase-negative staphylococci (CoNS) is the most common pathogen, and daptomycin has shown to be effective in treating it. The study demonstrates that daptomycin has a higher clinical cure rate in neonates with gram-positive infections.
Article
Agriculture, Dairy & Animal Science
M. Klempt, C. M. A. P. Franz, P. Hammer
Summary: This study highlights the potential presence of harmful bacteria in cheese, carrying antibiotic resistance genes and posing food safety concerns that warrant further evaluation.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
F. Ceciliani, M. Audano, M. F. Addis, C. Lecchi, M. H. Ghaffari, M. Albertini, F. Tangorra, R. Piccinini, D. Caruso, N. Mitro, V Bronzo
Summary: This observational study investigated the lipidome of cow milk during subclinical intramammary infection caused by non-aureus staphylococci using an untargeted approach. The results showed significant changes in lipid species belonging to triacylglycerols and sphingomyelins in quarter milk with NAS-IMI, potentially serving as novel biomarkers for improving mastitis diagnostics.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
H. Reydams, B. Toledo-Silva, K. Mertens, S. Piepers, F. N. de Souza, F. Haesebrouck, S. De Vliegher
Summary: This study investigated the distribution of non-aureus staphylococci and closely related mammaliicoccal species in composite cow milk and bulk-tank milk samples from commercial Flemish dairy herds. The results showed herd-specific NASM microbiota and variations within NASM species. Strain typing analysis provided insights into the origin and diversity of NASM species.
JOURNAL OF DAIRY SCIENCE
(2023)
Review
Food Science & Technology
Luc De Vuyst, Andrea Comasio, Simon Van Kerrebroeck
Summary: This article reviews the fermentation strategies and microbial ecology of sourdough production, with a focus on non-flour ingredients. Different types of sourdough production are classified based on the inoculum used. Traditional recipes for Type 1 sourdough often include non-flour ingredients as a microbial inoculum source or fermentation substrates.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Review
Biotechnology & Applied Microbiology
Cristian Diaz-Munoz, Luc De Vuyst
Summary: The application of starter culture mixture in cocoa fermentation plays a crucial role in improving the quality and flavor of chocolate products, making it essential to select appropriate strains for the process.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Microbiology
David Laureys, Frederic Leroy, Peter Vandamme, Luc De Vuyst
Summary: This study found that the backslopping time and rinsing of grains during water kefir fermentation have significant effects on the relative abundance of microbial communities and metabolites. Additionally, the temperature and rinsing of grains also affect the volumetric production rates of metabolites.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Cristian Diaz-Munoz, Dario Van de Voorde, Emmy Tuenter, Valerie Lemarcq, Davy Van de Walle, Jose Pedro Soares Maio, Alejandra Mencia, Carlos Eduardo Hernandez, Andrea Comasio, Eleni Sioriki, Stefan Weckx, Luc Pieters, Koen Dewettinck, Luc De Vuyst
Summary: This study examined the effects of Hanseniaspora opuntiae strain in cocoa fermentation. The results showed that the inoculated H. opuntiae strain was unable to dominate over other yeasts, resulting in under-fermented cocoa beans. However, cocoa fermentation processes inoculated with Saccharomyces cerevisiae strain produced richer and more reproducible aroma profiles.
Article
Microbiology
Louise Vermote, Marko Verce, Fernanda Mozzi, Luc De Vuyst, Stefan Weckx
Summary: The fiber, vitamin, and antioxidant contents in fruits are important for a balanced human diet. The microbiomes of the surfaces of tropical fruits from Northern Argentina showed considerable heterogeneity at the genus and species levels, with a significant proportion of unidentified microbial species. Fruit fermentation using autochthonous starter cultures can be a solution for reducing food waste.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Microbiology
Arne Bouchez, Luc De Vuyst
Summary: Beer is produced through a multistep brewing process, where certain bacteria can have different effects during fermentation and maturation. While bacteria can cause spoilage, they can also contribute to the desirable acidic flavors in certain sour beers.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Balazs Libisch, Sahabi Abdulkadir, Tibor Kereszteny, Peter P. Papp, Ferenc Olasz, Hedvig Febel, Zsuzsanna J. Sandor, Geertrui Rasschaert, Ellen Lambrecht, Marc Heyndrickx, Andras Szabo, Melinda Kovacs, Katalin Posta
Summary: This study aimed to analyze acquired antibiotic-resistance genes (ARGs) in the intestinal microbiome of two important food-animal species in Hungary from a One Health perspective. The results revealed different types of acquired ARGs in domestic pigs and carp, some of which have not been reported in Hungarian food animals. The presence of certain ARGs in both domestic and wild animal populations suggests a possible relationship.
Article
Multidisciplinary Sciences
Cristian Diaz-Munoz, Marko Verce, Luc De Vuyst, Stefan Weckx
Summary: This study unraveled the genome of the cocoa strain S. cerevisiae IMDO 050523 and found its close genetic relationship with a West African fermented food population. It also identified genetic signatures related to sucrose hydrolysis, pectin degradation, osmotolerance, and ester-producing enzymes, suggesting specific niche adaptations.
Review
Chemistry, Analytical
Stella Givanoudi, Marc Heyndrickx, Tom Depuydt, Mehran Khorshid, Johan Robbens, Patrick Wagner
Summary: This article provides an overview of biogenic amines (BAs) in the context of food quality and safety, focusing on their detection and quantification in analytical laboratories and on-site detection throughout the food chain. Various types of sensors, including chemosensors and bio/biomimetic sensors, are discussed, along with different receptors such as enzymes, antibodies, molecularly imprinted polymers (MIPs), and aptamers. The article concludes with an assessment of the limitations of BA sensors, a technological forecast, and thoughts on short-term solutions.
Article
Microbiology
Rudy Pelicaen, Stefan Weckx, Didier Gonze, Luc De Vuyst
Summary: In this study, a comparative genomic analysis was conducted on two candidate starter culture strains for cocoa fermentation, A. ghanensis LMG 23848(T) and A. senegalensis 108B, using genome sequence data. Genome-scale metabolic models (GEMs) were reconstructed to gain insights into their metabolic adaptations. The analysis revealed differences in carbon core metabolism and redox metabolism among Acetobacter species, providing new insights into cocoa fermentation and potential guidelines for future research.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
Louise Vermote, Jonas De Roos, Margo Cnockaert, Peter Vandamme, Stefan Weckx, Luc De Vuyst
Summary: Belgian lambic beers are produced using traditional craftsmanship, relying on wooden barrels for spontaneous fermentation and maturation. These barrels play a crucial role in establishing a stable microbial ecosystem, minimizing batch-to-batch variations, and preventing flavor deviations. They provide an inoculation source of necessary microorganisms and a microaerobic environment for successful lambic beer production.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Microbiology
Hannes Decadt, Stefan Weckx, Luc De Vuyst
Summary: This study evaluated the impact of three different starter culture mixtures on the organoleptic properties of Gouda cheeses. Analysis of 23 batches of cheeses at different ripening times revealed that different starter cultures led to variations in key metabolite concentrations and the relative abundance of non-starter lactic acid bacteria. Leuconostoc pseudomesenteroides played a minor role in aroma formation but had a major impact on the growth of non-starter lactic acid bacteria. The relative abundance of Tetragenococcus halophilus (high) and Loigolactobacillus rennini (low) increased from the rind to the core with ripening time, suggesting that a well-chosen T. halophilus strain could be used as an adjunct culture for Gouda cheese production.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Food Science & Technology
Celine Verdonck, Yamina De Bondt, Ines Pradal, An Bautil, Niels A. Langenaeken, Kristof Brijs, Peter Goos, Luc De Vuyst, Christophe M. Courtin
Summary: The final quality of wholemeal wheat bread is influenced by process parameter settings and leavening strategy. This study found that the specific volume of bread varied depending on the type of leavening strategy used, with a lower specific volume observed with sourdough compared to baker's yeast. The proofing time and water absorption had different effects on the specific volume when using different leavening strategies.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Plant Sciences
Ramon de Koning, Gertjan E. Wils, Raphael Kiekens, Luc De Vuyst, Geert Angenon
Summary: Due to climate change, farmers will face more extreme weather conditions and hence will need crops that are better adapted to these challenges. This study presents new insights into the role of raffinose family oligosaccharides (RFOs) as a resilience mechanism against environmental stress in common bean, particularly focusing on galactinol and RFOs in common beans exposed to drought and salt stress conditions.
Article
Ecology
Faizan Ahmed Sadiq, Koen De Reu, Hans Steenackers, Ann van de Walle, Mette Burmolle, Marc Heyndrickx
Summary: This study investigated interspecies interactions in mixed-species biofilms and revealed dynamic social interactions among bacteria, including commensalism, exploitation, and amensalism. The keystone species M. lacticum was found to enhance the growth of other species, leading to synergy in biofilm mass. The findings can be applied to cultivate, engineer, and design synthetic bacterial communities based on interaction-mediated approaches.
ISME COMMUNICATIONS
(2023)
