期刊
JOURNAL OF DAIRY SCIENCE
卷 93, 期 10, 页码 4625-4631出版社
ELSEVIER SCIENCE INC
DOI: 10.3168/jds.2010-3178
关键词
Prototheca zopfii genotype; Prototheca blaschkeae; genotype-specific PCR; analysis; milk
One hundred sixty-one Prototheca spp. strains isolated from composite milk and barn-surrounding environmental samples (bedding, feces, drinking, or washing water, surface swabs) of 24 Italian dairy herds were characterized by genotype-specific PCR analysis. Overall, 97.2% of strains isolated from composite milk samples were characterized as Prototheca zopfii genotype 2, confirming its role as the main mastitis pathogen, whereas Prototheca blaschkeae was only sporadically isolated (2.8%). Regarding environmental sampling, 84.9% of isolates belonged to P. zopfii genotype 2, 13.2% to P. blaschkeae, and 1.9% to P. zopfii genotype 1. The data herein contradict previous hypotheses about the supposed exclusive role of P. zopfii, genotype 2 as the causative agent of protothecal mastitis and, on the contrary; confirm the hypothesis that such pathology could be caused by P. blaschkeae in a few instances.
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