Article
Veterinary Sciences
Yu Jin Lee, Young Ju Lee
Summary: This study investigated the pathogenic potential of Coagulase-negative staphylococci (CoNS) isolated from bulk tank milk. The results showed that these bacteria exhibited a high prevalence of biofilm-forming ability and multidrug resistance. Therefore, developing a strong monitoring and sanitation program for dairy factories is crucial for ensuring hygienic milk production.
VETERINARY SCIENCES
(2022)
Article
Infectious Diseases
Nisa Sipahi, Ertugrul Kaya, Cansu Celik, Orhan Pinar
Summary: This study aimed to identify staphylococcal species in raw milk and determine their hemolysis, biofilm, coagulase activities, and β-lactam resistance. The results showed that these staphylococcal species exhibited certain virulence factors and antibiotic resistance genes, highlighting the need for continuous monitoring and attention to resistance concerns.
Article
Infectious Diseases
Anyaphat Srithanasuwan, Montira Intanon, Wasana Chaisri, Witaya Suriyasathaporn
Summary: The study aimed to determine the ability of coagulase-negative staphylococci (CNS) to survive in the same environment as major pathogens such as Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli. Results showed differences in the survival percentages among CNS species in all conditions. While most CNS strains could survive when cultured prior to major strains, some strains were able to degrade Streptococcus agalactiae. Although most CNS strains were degraded in the after and equal conditions, certain strains could resist Staphylococcus aureus and Streptococcus agalactiae. Research on these survival strains may suggest the possibility of novel bacteria with bacteriolytic capabilities as a new mastitis treatment.
Article
Agriculture, Dairy & Animal Science
H. Reydams, A. Wuytack, S. Piepers, K. Mertens, F. Boyen, F. N. de Souza, F. Haesebrouck, S. De Vliegher
Summary: This study assessed the genetic diversity of S. hominis isolated from different habitats and investigated their ability to utilize ferritin and lactoferrin as iron sources. The results showed that S. hominis strains can utilize ferritin to reverse the growth inhibition caused by iron deprivation.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Microbiology
Vanessa Silva, Elisete Correia, Jose Eduardo Pereira, Camino Gonzalez-Machado, Rosa Capita, Carlos Alonso-Calleja, Gilberto Igrejas, Patricia Poeta
Summary: This study evaluates the ability of biofilm formation in coagulase-negative staphylococci (CoNS) and S. pseudintermedius isolated from various animal species, and investigates the effect of conventional antimicrobials on reducing biofilm. The results show that all isolates can form biofilm, with S. urealyticus producing the most biomass and S. pseudintermedius producing the least biomass. Biofilm formation is positively associated with multidrug resistance and resistance to individual antimicrobials. Tetracycline and amikacin were unable to eradicate the biofilm, even at high concentrations.
Article
Chemistry, Applied
Li Yang, Hongjun Li, Han Wu, Shuyun Liu, Chang Su, Zhifei He
Summary: This study isolated and identified Coagulase-negative Staphylococci (CNS) from Chinese bacon, and evaluated their ability to degrade proteins and improve the taste of the bacon. Two CNS species were found to be capable of degrading proteins and enhancing the taste of Chinese bacon.
Article
Immunology
Filippo Marzoli, Barbara Turchi, Francesca Pedonese, Beatrice Torracca, Fabrizio Bertelloni, Giovanni Cilia, Domenico Cerri, Filippo Fratini
Summary: The study found that exposure to sub-inhibitory levels of disinfectants can affect their susceptibility as well as antibiotic resistance, particularly in qac-positive isolates and those harboring unexpressed antibiotic resistance genes.
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES
(2021)
Review
Microbiology
Angela Franca, Vania Gaio, Nathalie Lopes, Luis D. R. Melo
Summary: Coagulase-negative staphylococci (CoNS) have become major pathogens in healthcare-associated facilities, with a focus on biofilm formation and antibiotic resistance genes. These factors make treatment challenging, despite their lower virulence compared to S. aureus.
Article
Food Science & Technology
Wioleta Chajecka-Wierzchowska, Joanna Gajewska, Anna Zadernowska, Cinzia Lucia Randazzo, Cinzia Caggia
Summary: The present study aimed to evaluate and describe the diversity of CoNS strains as potential vectors for the transmission of antimicrobial resistance from RTE foods served in bars and restaurants. The study found that out of 85 CoNS strains, 67 strains were resistant to at least one antibiotic, with 37 strains exhibiting multidrug resistance (MDR-CoNS). Furthermore, CoNS strains carried resistance genes for antibiotics critically important in medicine, such as beta-lactams, aminoglycosides, and tetracyclines. The high frequency of MDR-CoNS in RTE foods poses a direct risk to public health as it contributes to the gene pool for acquisition of resistance traits by pathogenic bacteria.
Article
Microbiology
Vladimir Gostev, Semen Leyn, Alexander Kruglov, Daria Likholetova, Olga Kalinogorskaya, Marina Baykina, Natalia Dmitrieva, Zlata Grigorievskaya, Tatiana Priputnevich, Lyudmila Lyubasovskaya, Alexey Gordeev, Sergey Sidorenko
Summary: Coagulase-negative staphylococci (CoNS) were historically considered non-pathogenic, but strains such as S. epidermidis, S. haemolyticus, and S. hominis have now been identified as opportunistic pathogens. The off-label use of linezolid for CoNS infections has led to the emergence of international linezolid-resistant lineages. Genetic analysis revealed the presence of common ancestors for linezolid-resistant strains in different countries.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Luiza Pinheiro-Hubinger, Danilo Flavio Moraes Riboli, Ligia Maria Abraao, Eliane Patricia Lino Pereira Franchi, Maria de Lourdes Ribeiro de Souza da Cunha
Summary: This study compared the clonality and SCCmec type of CoNS isolates from different sources in the same community, revealing high clonal diversity in S. epidermidis and circulation of some S. haemolyticus clones in both hospital and community. The presence of different SCCmec types within the same cluster indicated high diversity, suggesting SCCmec may not be a good marker of clonality in these bacteria.
Article
Veterinary Sciences
Maria Filippa Addis, Clara Locatelli, Martina Penati, Sara Fusar Poli, Valentina Monistero, Lucia Zingale, Nicola Rota, Claudia Gusmara, Renata Piccinini, Paolo Moroni, Valerio Bronzo
Summary: Non-aureus staphylococci and mammaliicocci (NASM) are associated with bovine mastitis and increased milk somatic cell count (SCC). Regional differences have been observed in their prevalence. This retrospective study provides new insights into the NASM species isolated from cow milk in Italy and their relationships with mammary gland health in modern dairy farms.
VETERINARY RESEARCH COMMUNICATIONS
(2023)
Article
Infectious Diseases
Christine M. Mbindyo, George C. Gitao, Paul Joseph Plummer, Benard W. Kulohoma, Charles M. Mulei, Rawlynce Bett
Summary: The study revealed a high prevalence of multidrug-resistant staphylococci strains associated with mastitis in dairy cows in Kenya, posing a threat to both animal and human health. Different resistance genes and multidrug resistance were observed in both Staphylococcus aureus and coagulase-negative staphylococci species.
Article
Infectious Diseases
Yahya Mohzari, Fahad Aljobair, Ahmed Alrashed, Syed Mohammed Basheeruddin Asdaq, Renad Abdullah Alshuraim, Suzan Suhail Asfour, Mountasser Mohammad Al-Mouqdad, Reem F. Bamogaddam, Deemah Al-Anazi, Catherine E. Zeilinger, Ahmad Alamer, Batool Mohammed Alhassan, Nagaraja Sreeharsha
Summary: In recent years, there has been an increase in the prevalence of gram-positive bacteremia in neonates, but better care has also led to improved survival rates for premature neonates. Coagulase-negative staphylococci (CoNS) is the most common pathogen, and daptomycin has shown to be effective in treating it. The study demonstrates that daptomycin has a higher clinical cure rate in neonates with gram-positive infections.
Article
Agriculture, Dairy & Animal Science
M. Klempt, C. M. A. P. Franz, P. Hammer
Summary: This study highlights the potential presence of harmful bacteria in cheese, carrying antibiotic resistance genes and posing food safety concerns that warrant further evaluation.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Ecology
Aliona S. Rosca, Joana Castro, Angela Franca, Mario Vaneechoutte, Nuno Cerca
Summary: Bacterial vaginosis (BV) is a common bacterial vaginal infection with an unknown etiology, characterized by structured polymicrobial biofilms primarily formed by Gardnerella spp. and other anaerobic species. The study compared triple-species biofilms using pre-conditioned and competitive models, finding more significant synergistic growth interactions in the competitive model. Despite model differences, G. vaginalis consistently dominated the biofilm composition, emphasizing its relevance in BV.
Article
Microbiology
Christine Landlinger, Vera Oberbauer, Lenka Podpera Tisakova, Timo Schwebs, Rocio Berdaguer, Leen Van Simaey, Mario Vaneechoutte, Lorenzo Corsini
Summary: This study investigates the potential mechanisms of therapy failure in bacterial vaginosis treatment, revealing that metronidazole and clindamycin face issues of resistance formation and ineffectiveness on biofilm. However, a novel endolysin PM-477 shows high effectiveness in eliminating Gardnerella and has high resilience to resistance formation, making it a promising alternative for the treatment of bacterial vaginosis.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Review
Veterinary Sciences
R. N. Zadoks, E. Scholz, S. M. Rowe, J. M. Norris, H. B. Pooley, J. House
Summary: There are many culture-based diagnostics available for on-farm detection of bacterial pathogens in milk, which may help improve antimicrobial use in udder health management. However, farms have limited diagnostic microbiology capacity. The World Health Organisation has established criteria called ASSURED for evaluating diagnostic tests in low-resource settings, and this article reviews how these criteria can be applied to the diagnosis of mastitis pathogens and the performance of on-farm diagnostics in Australia. The evaluation reveals trade-offs and highlights the importance of considering the specific needs and characteristics of each farm.
AUSTRALIAN VETERINARY JOURNAL
(2023)
Article
Multidisciplinary Sciences
Shazia Bashir, Nguyen Ngoc N. Phuoc, Tharangani Herath, Abdul Basit, Ruth Zadoks, Sudaxshina Murdan
Summary: Intensive tilapia farming has led to the emergence of novel pathogens, including Streptococcus agalactiae (GBS) which caused the first outbreak of foodborne GBS illness in humans. A proof-of-concept study developed an oral vaccine that provided significant protection from GBS in tilapia, showing promise for future use against other bacterial pathogens and fish species.
Review
Biochemistry & Molecular Biology
Junhua Xie, Freddy Haesebrouck, Lien Van Hoecke, Roosmarijn E. Vandenbroucke
Summary: Bacterial extracellular vesicles (bEVs) have been shown to play a crucial role in the pathogenesis of various diseases. This article thoroughly discusses the contribution of bEVs in disease pathogenesis, their potential as diagnostic biomarkers, and their application as therapeutic targets.
TRENDS IN MICROBIOLOGY
(2023)
Review
Fisheries
Olga L. M. Haenen, Ha Thanh Dong, Truong Dinh Hoai, Margaret Crumlish, Iddya Karunasagar, Timothy Barkham, Swaine L. Chen, Ruth Zadoks, Andreas Kiermeier, Bing Wang, Esther Garrido Gamarro, Masami Takeuchi, Mohammad Noor Amal Azmai, Belen Fouz, Rolando Pakingking, Zeng Wei Wei, Melba G. Bondad-Reantaso
Summary: Tilapia culture is a significant source of income and nutrition for rural families. However, intensification and global warming have led to bacterial disease outbreaks in tilapia farms, threatening sustainable production. This review provides an overview of common and emerging bacterial pathogens, diseases, diagnostics, and the risks of antimicrobial resistance in tilapia.
REVIEWS IN AQUACULTURE
(2023)
Article
Microbiology
Wanna Sirimanapong, Nguyen Ngoc Phuoc, Chiara Crestani, Swaine Chen, Ruth N. Zadoks
Summary: Group B Streptococcus (GBS) is a major pathogen in humans and aquatic species, and the ST283 sequence type has been identified as the cause of severe foodborne GBS disease in healthy adults in Southeast Asia. The distribution of potentially human-pathogenic GBS in aquaculture species is poorly known, and further research is needed.
Article
Infectious Diseases
Francisco Cortez Nunes, Emily Taillieu, Teresa Letra Mateus, Silvia Teixeira, Freddy Haesebrouck, Irina Amorim
Summary: This study aimed to assess the presence of antibiotic resistance genes previously reported in Helicobacter pylori in gastric samples from 36 pigs where DNA of H. pylori-like organisms had been detected. Based on PCR and sequencing analysis, two samples were positive for the 16S rRNA mutation gene conferring tetracycline resistance, and one sample was positive for the frxA gene conferring metronidazole resistance. These findings indicate the potential occurrence of acquired antimicrobial resistance in H. pylori-like organisms associated with pigs.
Article
Veterinary Sciences
S. Rowe, C. Cunningham, L. Ingenhoff, J. M. Norris, R. N. Zadoks
Summary: This study aimed to determine the presence of MRSA, ESBL-producing Enterobacteriaceae, and VRE in bulk tank milk in NSW, Australia. No samples tested positive for these antimicrobial resistant organisms, indicating a low prevalence in dairy herds in NSW.
AUSTRALIAN VETERINARY JOURNAL
(2023)
Article
Veterinary Sciences
Francisco Cortez Nunes, Teresa Letra Mateus, Emily Taillieu, Silvia Teixeira, Nuno Carolino, Alexandra Rema, Sofie De Bruyckere, Fatima Gartner, Freddy Haesebrouck, Irina Amorim
Summary: This study reveals the presence of Helicobacter species and F. gastrosuis in the stomach of pigs and wild boars, indicating a possible association with gastric disorders.
VETERINARY RESEARCH
(2022)
Meeting Abstract
Gastroenterology & Hepatology
Emily Taillieu, Sophie Rutten, Stijn Michiels, Yuna Arnst, Sofie De Bruyckere, Koen Chiers, Frauke van Aert, Heiko De Schepper, Emma Callewaert, Christophe George, Wouter Van Moerkercke, Glenn Vanneste, Nina Van Heddegem, Erik Vanderstraeten, Freddy Haesebrouck, Christophe Van Steenkiste
Review
Veterinary Sciences
Emily Taillieu, Koen Chiers, Irina Amorim, Fatima Gaertner, Dominiek Maes, Christophe Van Steenkiste, Freddy Haesebrouck
Summary: This article discusses the importance of Helicobacter species in dogs, cats, and pigs and their potential to infect humans. The article highlights the association of Helicobacter suis with gastritis and decreased weight gain in pigs, as well as its potential to cause disease in humans. It also mentions that multiple Helicobacter species can colonize the stomachs of dogs and cats, but their pathogenic significance is likely low. The article concludes that besides H. pylori, gastric Helicobacter species associated with these animals are also clinically relevant in humans.
VETERINARY RESEARCH
(2022)
Review
Gastroenterology & Hepatology
L. Capirchio, T-D. Huang, C. De Witte, F. Haesebrouck, C. Fervaille, C. Gillain, J-F. Rahier, T. De Ronde
Summary: This article reports the first case of elevated CA 19-9 related to H. suis gastritis, which normalized after eradication. In addition to H. pylori, infection with NHPH species can also cause elevated CA 19-9.
ACTA GASTRO-ENTEROLOGICA BELGICA
(2022)
Article
Veterinary Sciences
Silvia Teixeira, Dulce Filipe, Manuela Cerqueira, Patricia Barradas, Francisco Cortez Nunes, Fatima Faria, Freddy Haesebrouck, Joao R. Mesquita, Fatima Gartner, Irina Amorim
Summary: Cats serve as a natural reservoir for many non-Helicobacter pylori Helicobacters, and close contact with animals has been identified as a risk factor for gastric diseases caused by these bacteria. The prevalence of Helicobacter species in cats was determined, with Helicobacter heilmannii and Helicobacter salomonis being the most commonly detected species. Significant histopathological alterations associated with the presence of Helicobacter spp. were not observed in most cases, suggesting potential adaptation of these bacteria to the feline gastric microenvironment.
VETERINARY SCIENCES
(2022)
Article
Veterinary Sciences
Karina Sonalio, Henrique M. S. Almeida, Marina L. Mechler-Dreibi, Gabriel Y. Storino, Freddy Haesebrouck, Dominiek Maes, Luis Guilherme de Oliveira
Summary: The study found that Mycoplasma hyopneumoniae infection affects the diversity of respiratory microbiota and facilitates colonization of other pathogens. Differences in pulmonary and nasal microbiota were observed in pigs with different infection statuses.
VETERINARY RESEARCH
(2022)