Article
Immunology
Barbara Rodriguez-Urretavizcaya, Nuria Pascual, Carme Pastells, Maria Teresa Martin-Gomez, Lluisa Vilaplana, Maria-Pilar Marco
Summary: A new immunochemical assay for detecting the important virulence factor pyocyanin in Pseudomonas aeruginosa has been developed with high sensitivity, achieving a much lower limit of detection than concentrations found in clinical samples. The study reveals potential for using pyocyanin as a biomarker for patient stratification in P. aeruginosa infections, showing significant differences in pyocyanin levels between clinical isolates from patients with acute versus chronic infections.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Amr A. Baiomy, Fathy E. Serry, Ashraf A. Kadry, Galal Yahya, Swapnil Doijad, Ahmed Mostafa, Mobarak Abu Mraheil, Amira M. El-Ganiny
Summary: This study conducted genomic analyses of Pseudomonas aeruginosa persister isolates from chronic human infections, revealing their low metabolic rates, multidrug tolerance, and strong biofilm-forming ability. Whole genome sequencing and phylogenetic analysis showed that these persister isolates belong to a distinct clade with a smaller genome size.
Article
Respiratory System
Jonathan D. Cogen, Matthew Hall, Anna V. Faino, Lilliam Ambroggio, Anne J. Blaschke, Thomas V. Brogan, Jillian M. Cotter, Ronald L. Gibson, Carlos G. Grijalva, Adam L. Hersh, Susan C. Lipsett, Samir S. Shah, Daniel J. Shapiro, Mark I. Neuman, Jeffrey S. Gerber
Summary: This study evaluated the clinical outcomes of adding anti-MRSA antibiotics to antipseudomonal antibiotic treatment for children with CF co-infected with MRSA and Pa. The results showed that the addition of anti-MRSA antibiotics did not improve lung function or reduce the need for future IV antibiotic treatment compared to antipseudomonal antibiotics alone.
JOURNAL OF CYSTIC FIBROSIS
(2023)
Article
Infectious Diseases
Daria Plokarz, Karolina Bierowiec, Krzysztof Rypula
Summary: This study investigated 271 strains of Pseudomonas aeruginosa collected from dogs and cats for antibiotic resistance and the presence of virulence factor genes. The most commonly detected resistance was to fluoroquinolones, and the highest prevalence of virulence genes was found for exoT and lasB. Associations between the presence of certain genes, such as toxA and exoU, and resistance to specific antibiotics were observed. These findings indicate the need for further studies to understand the relationship between antibiotic resistance and virulence in P. aeruginosa.
Article
Respiratory System
Laurent Meijer, Genevieve Hery-Arnaud, Cyril Leven, Emmanuel Nowak, Sophie Hillion, Yves Renaudineau, Isabelle Durieu, Raphael Chiron, Anne Prevotat, Isabelle Fajac, Dominique Hubert, Marlene Murris-Espin, Sandrine Huge, Isabelle Danner-Boucher, Bruno Ravoninjatovo, Sylvie Leroy, Julie Macey, Thierry Urban, Gilles Rault, Dominique Mottier, Rozenn Le Berre
Summary: The orally available kinase inhibitor R-roscovitine has shown safety and tolerability in CF patients, but did not demonstrate significant efficacy in terms of clinical outcomes. The lack of evidence for efficacy may be attributed to high pharmacokinetics variability, short duration of treatment, and/or inappropriate dosing protocol.
JOURNAL OF CYSTIC FIBROSIS
(2022)
Article
Respiratory System
Laurent Meijer, Genevieve Hery-Arnaud, Cyril Leven, Emmanuel Nowak, Sophie Hillion, Yves Renaudineau, Isabelle Durieu, Raphael Chiron, Anne Prevotat, Isabelle Fajac, Dominique Hubert, Marlene Murris-Espin, Sandrine Huge, Isabelle Danner-Boucher, Bruno Ravoninjatovo, Sylvie Leroy, Julie Macey, Thierry Urban, Gilles Rault, Dominique Mottier, Rozenn Le Berre
Summary: A study was conducted to evaluate the safety and effects of roscovitine in patients with Cushing disease. The results showed that roscovitine was relatively safe and well-tolerated, but did not show significant efficacy in treating the disease. The variability in pharmacokinetics of roscovitine may have contributed to the lack of effectiveness.
JOURNAL OF CYSTIC FIBROSIS
(2022)
Article
Respiratory System
Janardan P. Pandey, Aryan M. Namboodiri, Paul J. Nietert, Michael R. Knowles, Rhonda G. Pace, Gerald B. Pier
Summary: The study found that immunoglobulin GM and KM genotypes influence the humoral immune response to LPS O, alginate, and PNAG antigens in CF patients, potentially offering novel immunotherapeutic approaches against P. aeruginosa.
JOURNAL OF CYSTIC FIBROSIS
(2021)
Article
Otorhinolaryngology
Helene Stampe, Kathrine Kronberg Jakobsen, Jesper Filtenborg Tvedskov, Irene Wessel, Katalin Kiss, Jeppe Friborg, Amanda Oester Andersen, Christian Gronhoj, Christian von Buchwald, Anders Christensen
Summary: This study aims to investigate the thresholds for lymph node yield, lymph node density, and pN in patients with oral squamous cell carcinoma and their relation to survival outcomes. The results suggest that increased nodal yield, decreased LND, and decreasing number of pN are associated with significantly improved survival outcomes.
OTOLARYNGOLOGY-HEAD AND NECK SURGERY
(2023)
Article
Allergy
Sven F. Seys, Peter W. Hellings, Isam Alobid, Vibeke Backer, Emilie Bequignon, Christian von Buchwald, Carlo Cavaliere, Andre Coste, Lauren Deneyer, Zuzana Diamant, Julia Eckl-Dorna, Wytske J. Fokkens, Simon Gane, Philippe Gevaert, Christiane Holbaek-Haase, Clemens Holzmeister, Claire Hopkins, Valerie Hox, Caroline Huart, Roger Jankowski, Mark Jorissen, Anette Kjeldsen, Lisa Knipps, Bibi Lange, Rik van der Lans, Anu Laulajainen-Hongisto, Kenneth Larsen, David T. Liu, Valerie Lund, Gert Marien, Simonetta Masieri, Geoffrey Mortuaire, Joaquim Mullol, Sietze Reitsma, Philippe Rombaux, Sven Schneider, Andreas Steinsvik, Peter-Valentin Tomazic, Sanna K. Toppila-Salmi, Laura Van Gerven, Thibaut Van Zele, Paula Virkkula, Martin Wagenmann
Summary: This study aims to establish an international chronic rhinosinusitis outcome registry based on real-world data collection enabled by mobile health technology. A collaborative scientific network of 17 university ear-nose-throat clinics from 10 European countries has been established to collect data in a longitudinal and standardized manner. Up to 300 patients have already been included.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY-IN PRACTICE
(2023)
Review
Virology
Seyed Keybud Katirachi, Mathias Peter Gronlund, Kathrine Kronberg Jakobsen, Christian Gronhoj, Christian von Buchwald
Summary: This study aimed to determine the prevalence of human papillomavirus (HPV) infection in oral cavity squamous cell carcinomas (OCSCC). A meta-analysis of 31 studies including 5007 patients revealed an overall HPV+ OCSCC prevalence of 6% (95% CI; 3-10%). The results suggest that HPV may not be a necessary or strong risk factor in OCSCC oncogenesis.
Article
Virology
Marius Meldgaard Justesen, Kathrine Kronberg Jakobsen, Simone Kloch Bendtsen, Martin Garset-Zamani, Christine Mordhorst, Amanda-Louise Fenger Carlander, Anita Birgitte Gothelf, Christian Gronhoj, Christian von Buchwald
Summary: This study investigated the prognostic impact of neutrophil-to-lymphocyte ratio (NLR) on overall survival (OS) and recurrence-free survival (RFS) in patients with oropharyngeal squamous cell carcinoma (OPSCC). The results showed that an increasing NLR was associated with a worse outcome in terms of both OS and RFS, independently of HPV status.
Article
Virology
Lasse Andersen, Kathrine Kronberg Jakobsen, Amanda-Louise Fenger Carlander, Martin Garset-Zamani, Jeppe Friborg, Katalin Kiss, Rasmus L. Marvig, Caroline Olsen, Finn Cilius Nielsen, Elo Andersen, Christian Gronhoj, Christian von Buchwald
Summary: This study examined the risk of second primary cancer (SPC) following oropharyngeal squamous cell carcinoma (OPSCC) and the impact of human papillomavirus (HPV) on survival following SPC. The risk of SPC was approximately five times higher than the general population, and HPV-positive (HPV+) patients had a longer time to SPC and better survival compared to HPV-negative (HPV-) patients. SPC was most frequently found in the lungs, head, and neck (LHN) for HPV- OPSCC patients and lungs followed by gender-specific sites for HPV+ OPSCC.
Letter
Otorhinolaryngology
Martin Garset-Zamani, Christian von Buchwald, Gunnhild Karevold, Tina Klitmoeller Agander, Angels Mateu-Regue, Hanne Astrid Eide, Jesper Filtenborg Tvedskov
Summary: This case report discusses a rare occurrence of a young Norwegian woman in her early 20s developing human papillomavirus-positive oropharyngeal squamous cell carcinoma at an extremely early age.
JAMA OTOLARYNGOLOGY-HEAD & NECK SURGERY
(2023)
Letter
Oncology
Benedicte Bitsch Lauritzen, Sannia Sjostedt, Jakob Myllerup Jensen, Katalin Kiss, Christian von Buchwald
Article
Microbiology
Eva Armengol, Kasper Norskov Kragh, Tim Tolker-Nielsen, Josep M. Sierra, Doaa Higazy, Oana Ciofu, Miguel Vinas, Niels Hoiby
Summary: The combination of colistin and rifampicin has been found to effectively kill colistin-resistant Pseudomonas aeruginosa, suggesting it as a promising treatment for infections caused by this strain.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Otorhinolaryngology
Eva Lykke, Anders Christensen, Karina Juhl, Ulla Feldt-Rasmussen, Mette Friberg Hitz, Sannia Mia Svenningsen Sjostedt, Christoffer Holst Hahn, Ditte Maria Kraik Svensson, Karoline Kanstrup Springborg, Mads Georg Stage, Gitte Bjorn Hvilsom, Linda Maria Hilsted, Morten Dahl, Giedrius Lelkaitis, Andreas Kjaer, Preben Homoe, Christian von Buchwald
Summary: The purpose of this study was to investigate whether near-infrared autofluorescence image guidance can reduce the incidence of hypoparathyroidism in total thyroidectomy. The results showed that near-infrared autofluorescence imaging can improve the detection rate of parathyroid glands and reduce the rate of hypoparathyroidism, but the difference was not statistically significant. Further studies are needed to confirm the use of near-infrared autofluorescence imaging as an auxiliary tool for parathyroid gland identification.
EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY
(2023)
Article
Otorhinolaryngology
Anders Christensen, Irene Wessel, Birgitte Wittenborg Charabi, Karina Juhl, Katalin Kiss, Giedrius Lelkaitis, Jann Mortensen, Andreas Kjaer, Christian von Buchwald, Jesper Filtenborg Tvedskov
Summary: The aim of this study was to investigate the diagnostic performance of bimodal optical and radio-guided sentinel node biopsy (SNB) for oral squamous cell carcinoma (OSCC) sub-sites in the anterior oral cavity. A total of 50 cN0 OSCC patients scheduled for SNB were prospectively studied and injected with the tracer complex Tc99m:ICG:Nacocoll. Optical imaging appeared to be an effective tool for real-time SN identification unaffected by possible interference from the injection.
EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Mads Lawaetz, Anders Christensen, Karina Juhl, Kirstine Karnov, Giedrius Lelkaitis, Anne-Marie Kanstrup Fiehn, Andreas Kjaer, Christian von Buchwald
Summary: There is currently no approved tumor-specific imaging agents for head and neck cancer. This study explored the expression of nine imaging targets in oral squamous cell carcinoma (OSCC) patients to identify potential molecular imaging targets. The urokinase-type plasminogen activator receptor (uPAR), integrin alpha v beta 6, and tissue factor showed high expression rates and are promising imaging targets for OSCC primary tumors, lymph node metastases, and recurrences.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Kathrine K. Jakobsen, Simone K. Bendtsen, Niels Pallisgaard, Jeppe Friborg, Giedrius Lelkaitis, Christian Gronhoj, Christian von Buchwald
Summary: This study aims to evaluate the accuracy of cell-free human papillomavirus-DNA (cfHPV-DNA) measurements in liquid biopsies and found a high sensitivity in predicting disease in patients with HPV-positive/p16-positive oropharyngeal squamous cell carcinoma (OPSCC). Serial plasma HPV samples were recommended for clinical monitoring of these patients.
CLINICAL CANCER RESEARCH
(2023)
Article
Medicine, General & Internal
Mads Lawaetz, Anders Christensen, Karina Juhl, Giedrius Lelkaitis, Kirstine Karnov, Esben Andreas Carlsen, Birgitte W. Charabi, Annika Loft, Dorota Czyzewska, Christian von Buchwald, Andreas Kjaer
Summary: This study investigated the diagnostic value of uPAR-PET/CT in detecting regional metastatic disease in patients with oral and oropharyngeal squamous cell carcinoma. The results showed that uPAR-PET/CT had a sensitivity of 56% and specificity of 100% in detecting metastatic disease. Compared to conventional imaging, uPAR-PET/CT was able to upstage patients with occult metastases and increase the sensitivity to 64%.
Article
Microbiology
Anne Sofie Laulund, Franziska Angelika Schwartz, Lars Christophersen, Mette Kolpen, Peter Ostrup Jensen, Henrik Calum, Niels Hoiby, Kim Thomsen, Claus Moser
Summary: Chronic wounds have compromised microcirculation and are often infested with microorganisms in biofilms, hindering antibiotic function. This study investigates whether hyperbaric oxygen therapy (HBOT) can counteract this process.
Article
Microbiology
Anne Sofie Laulund, Franziska Angelika Schwartz, Niels Hoiby, Kim Thomsen, Claus Moser
Summary: The study demonstrates that hyperbaric oxygen therapy (HBOT) can enhance the effectiveness of tobramycin in biofilms and counteract the micro-compartmentalization of biofilm pharmacokinetics through increased available tobramycin and augmented bacterial killing.