Article
Biochemistry & Molecular Biology
Nguyen Bao Vy Tran, Quang Minh Truong, Lam Que Anh Nguyen, Ngoc My Huong Nguyen, Quang Hung Tran, Thi Tuyet Phuong Dinh, Vinh Son Hua, Van Dung Nguyen, Peter A. Lambert, Thi Thu Hoai Nguyen
Summary: Understanding the colonization of P. aeruginosa in healthy humans is important for future prevention and treatment. This study investigated its prevalence and risk factors in 609 Vietnamese volunteers. It was found that 3.12% of participants were colonized by P. aeruginosa, with a higher likelihood in those with a history of sinusitis.
Article
Respiratory System
Wang Chun Kwok, James Chung Man Ho, Terence Chi Chun Tam, Mary Sau Man Ip, David Chi Leung Lam
Summary: The study revealed that patients with longer duration of non-cystic fibrosis bronchiectasis and those on proton pump inhibitors (PPIs) are more likely to have Pseudomonas aeruginosa colonization, leading to more extensive lung involvement and higher risks of exacerbation requiring hospitalization.
RESPIRATORY RESEARCH
(2021)
Article
Medicine, General & Internal
Anna Lisa Montemari, Valeria Marzano, Nour Essa, Stefano Levi Mortera, Martina Rossitto, Simone Gardini, Laura Selan, Gianluca Vrenna, Andrea Onetti Muda, Lorenza Putignani, Ersilia Vita Fiscarelli
Summary: Cystic fibrosis (CF) is a common rare disease caused by a mutation in the CFTR gene, leading to mucus accumulation and recurrent respiratory infections. Pseudomonas aeruginosa (PA) is the most detected bacterium in CF patients and is able to adapt and cause chronic infections in the hostile environment of the CF lung. This study compared surface protein expression of antibiotic-resistant strains of PA to antibiotic-sensitive strains and characterized their biofilm production.
FRONTIERS IN MEDICINE
(2022)
Review
Microbiology
Christina S. Thornton, Michael G. Surette
Summary: Cystic fibrosis is the most common and lethal genetic disease among the Caucasian population, leading to chronic airway inflammation and declining pulmonary function. Studies have shown a diverse community of anaerobic bacteria in CF patients' airways, potentially impacting disease progression through synergistic interaction with principal pathogens. Despite the understanding of this complex bacterial milieu, the specific roles of anaerobes in disease progression remain unclear.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Microbiology
Xuerui Bao, Mona Bove, Tom Coenye
Summary: The high tolerance of Pseudomonas aeruginosa biofilms in respiratory tract infections in cystic fibrosis contributes to the failure of antibiotic therapy. This study identified carbon sources that could enhance the inhibiting and/or eradicating activity of tobramycin, ciprofloxacin, and ceftazidime against P. aeruginosa PAO1 biofilms. The mechanisms underlying the enhanced biofilm eradicating activity were strain-dependent.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Review
Immunology
Veronica Roxana Flores-Vega, Silvia Yalid Vargas-Roldan, Jose Luis Lezana-Fernandez, Ricardo Lascurain, Jose Ignacio Santos-Preciado, Roberto Rosales-Reyes
Summary: Cystic fibrosis is a genetic disease caused by a mutation in the cftr gene, affecting chloride ion and water transport and leading to bacterial infections in the lungs. Autophagy plays a crucial role in pathogen clearance, but P. aeruginosa and B. cenocepacia have strategies to evade this pathway, resulting in chronic inflammatory immune responses.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Jonathan D. Cogen, Anna Faino, Frankline Onchiri, Lucas R. Hoffman, Matthew P. Kronman, David P. Nichols, Margaret Rosenfeld, Ronald L. Gibson
Summary: A retrospective cohort study found that there were no significant differences in respiratory and clinical outcomes between young patients with cystic fibrosis treated with one versus two intravenous antipseudomonal antibiotics for pulmonary exacerbations.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Giovanni Di Bonaventura, Veronica Lupetti, Simone De Fabritiis, Alessandra Piccirilli, Annamaria Porreca, Marta Di Nicola, Arianna Pompilio
Summary: This study evaluated the antibacterial and antivirulence activities of eight FDA-approved non-antibiotic drugs and their effects on biofilm formation. Ciclopirox and actinomycin D showed the best antibacterial activity and were most effective against preformed biofilms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Medicine, Research & Experimental
Roberto Rosales-Reyes, Veronica Roxana Flores-Vega, Jose Luis Lezana-Fernandez, Jose Ignacio Santos-Preciado
Summary: Pseudomonas aeruginosa is a significant pathogen causing lung infections in patients with cystic fibrosis. Its increasing antibiotic resistance makes treatment challenging. Microbiology and whole genome sequencing are important tools for determining antimicrobial susceptibility and resistance as well as analyzing genomic variations. Molecular epidemiologic surveillance aids in developing better treatment strategies and controlling the spread of Pseudomonas aeruginosa variants.
ARCHIVES OF MEDICAL RESEARCH
(2022)
Article
Immunology
Jonathan D. Cogen, Frankline M. Onchiri, Nicole Mayer Hamblett, Ronald L. Gibson, Wayne J. Morgan, Margaret Rosenfeld
Summary: The study showed that prolonged use of oral antibiotics in children with cystic fibrosis increased the risk of acquiring Achromobacter xylosoxidans, while treatment with intravenous antibiotics was associated with an increased risk of acquiring multidrug-resistant Pseudomonas aeruginosa and MRSA.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Microbiology
Giovanni Di Bonaventura, Veronica Lupetti, Fabio Verginelli, Sara Giancristofaro, Rosemary Barbieri, Giovanni Gherardi, Arianna Pompilio
Summary: This study evaluated the antibacterial, antibiofilm, and antivirulence activities of apramycin compared to tobramycin against P. aeruginosa in cystic fibrosis patients. Apramycin showed better efficacy than tobramycin, without cytotoxicity to bronchial epithelial CF cells.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Nutrition & Dietetics
Aleksandra John, Joanna Gozdzik-Spychalska, Magdalena Durda-Masny, Wojciech Czainski, Natalia Pawlowska, Jolanta Wlizlo, Halina Batura-Gabryel, Anita Szwed
Summary: The study described the relationship between the prevalence of Pseudomonas aeruginosa (PA) and lung function, nutritional status, and gene mutation type in adult patients with cystic fibrosis (CF). The presence of PA was associated with lower FEV1% and BMI values. Severe gene mutation, undernutrition, and deterioration of lung function were linked to a higher probability of PA acquisition and resistance to antibiotic treatment.
Article
Medicine, General & Internal
Layla Diab Caceres, Ester Zamarron de Lucas
Summary: Cystic fibrosis is a genetic multisystemic disease with respiratory involvement as the main comorbidity in adulthood. The advent of cystic fibrosis transmembrane conductance regulator (CFTR) modulators has changed the treatment paradigm by improving defective CFTR protein production and expression, thus enhancing ion exchange and reducing airflow obstruction.
Review
Medicine, Research & Experimental
Roberto Rosales-Reyes, Silvia Yalid Vargas-Roldan, Jose Luis Lezana-Fernandez, Jose Ignacio Santos-Preciado
Summary: Cystic fibrosis is a genetic disease that affects the respiratory and digestive systems, causing chronic lung infections and breathing difficulties. Mutations in the CFTR gene lead to imbalanced intracellular water content, resulting in sticky mucus and bacterial infections that ultimately affect lung function.
ARCHIVES OF MEDICAL RESEARCH
(2021)
Article
Biology
Jonas Maurice Holzinger, Martina Toelge, Maren Werner, Katharina Ursula Ederer, Heiko Ingo Siegmund, David Peterhoff, Stefan Helmut Blaas, Nicolas Gisch, Christoph Brochhausen, Andre Gessner, Sigrid Buelow
Summary: Chronic pulmonary infection in cystic fibrosis patients requires continuous antibiotic treatment. Pseudomonas aeruginosa (Pa) is a major concern due to its frequent acquisition of multiple drug resistance (MDR). Neutrophil-derived bactericidal/permeability-increasing protein (BPI) has high bactericidal potency against Gram-negative bacteria, but its function is neutralized by anti-neutrophil cytoplasmic antibodies against BPI (BPI-ANCA) in a significant number of cystic fibrosis patients.
Article
Ophthalmology
Marina Knudsen Kirkegaard, Marthe Minderman, Lene Dissing Sjo, Steven T. Pals, Patrick R. G. Eriksen, Steffen Heegaard
Summary: This study aimed to reclassify ocular adnexal large B-cell lymphomas (OA-LBCLs) and investigate the prevalence and association of MYD88 and CD79B mutations with clinical parameters. The results revealed that OA-LBCL mainly consists of two subtypes, and MYD88 mutations, as well as cMYC/BCL2 double-expressor DLBCL, are associated with poor prognosis.
BRITISH JOURNAL OF OPHTHALMOLOGY
(2023)
Review
Allergy
Christian Korsgaard Pedersen, Christiane Haase, Kasper Aanaes, Christian von Buchwald, Vibeke Backer
Summary: Patient reported outcome measures (PROMs) are important in assessing global airway disease caused by type-2 inflammation, as they provide insights into patients' perspective on symptoms, treatment, disease control, and possible comorbidity. This review investigates newly developed PROMs for global airway disease and provides an overview of commonly used PROMs in managing the condition.
CURRENT OPINION IN ALLERGY AND CLINICAL IMMUNOLOGY
(2023)
Letter
Otorhinolaryngology
Martin Garset-Zamani, Christian von Buchwald, Gunnhild Karevold, Tina Klitmoeller Agander, Angels Mateu-Regue, Hanne Astrid Eide, Jesper Filtenborg Tvedskov
Summary: This case report discusses a rare occurrence of a young Norwegian woman in her early 20s developing human papillomavirus-positive oropharyngeal squamous cell carcinoma at an extremely early age.
JAMA OTOLARYNGOLOGY-HEAD & NECK SURGERY
(2023)
Article
Otorhinolaryngology
Eva Lykke, Anders Christensen, Karina Juhl, Ulla Feldt-Rasmussen, Mette Friberg Hitz, Sannia Mia Svenningsen Sjostedt, Christoffer Holst Hahn, Ditte Maria Kraik Svensson, Karoline Kanstrup Springborg, Mads Georg Stage, Gitte Bjorn Hvilsom, Linda Maria Hilsted, Morten Dahl, Giedrius Lelkaitis, Andreas Kjaer, Preben Homoe, Christian von Buchwald
Summary: The purpose of this study was to investigate whether near-infrared autofluorescence image guidance can reduce the incidence of hypoparathyroidism in total thyroidectomy. The results showed that near-infrared autofluorescence imaging can improve the detection rate of parathyroid glands and reduce the rate of hypoparathyroidism, but the difference was not statistically significant. Further studies are needed to confirm the use of near-infrared autofluorescence imaging as an auxiliary tool for parathyroid gland identification.
EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY
(2023)
Article
Otorhinolaryngology
Anders Christensen, Irene Wessel, Birgitte Wittenborg Charabi, Karina Juhl, Katalin Kiss, Giedrius Lelkaitis, Jann Mortensen, Andreas Kjaer, Christian von Buchwald, Jesper Filtenborg Tvedskov
Summary: The aim of this study was to investigate the diagnostic performance of bimodal optical and radio-guided sentinel node biopsy (SNB) for oral squamous cell carcinoma (OSCC) sub-sites in the anterior oral cavity. A total of 50 cN0 OSCC patients scheduled for SNB were prospectively studied and injected with the tracer complex Tc99m:ICG:Nacocoll. Optical imaging appeared to be an effective tool for real-time SN identification unaffected by possible interference from the injection.
EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Mads Lawaetz, Anders Christensen, Karina Juhl, Kirstine Karnov, Giedrius Lelkaitis, Anne-Marie Kanstrup Fiehn, Andreas Kjaer, Christian von Buchwald
Summary: There is currently no approved tumor-specific imaging agents for head and neck cancer. This study explored the expression of nine imaging targets in oral squamous cell carcinoma (OSCC) patients to identify potential molecular imaging targets. The urokinase-type plasminogen activator receptor (uPAR), integrin alpha v beta 6, and tissue factor showed high expression rates and are promising imaging targets for OSCC primary tumors, lymph node metastases, and recurrences.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Hisham Mehanna, Miren Taberna, Christian von Buchwald, Sara Tous, Jill Brooks, Marisa Mena, Francisca Morey, Christian Gronhoj, Jacob Hoygaard Rasmussen, Martin Garset-Zamani, Laia Bruni, Nikolaos Batis, Ruud H. Brakenhoff, C. Rene Leemans, Robert J. Baatenburg de Jong, Jens Peter Klussmann, Nora Wuerdemann, Steffen Wagner, Tina Dalianis, Linda Marklund, Haitham Mirghani, Andrew Schache, Jaqueline A. James, Shao Hui Huang, Brian O'Sullivan, Paul Nankivell, Martina A. Broglie, Markus Hoffmann, Elgar Susanne Quabius, Laia Alemany
Summary: The study aimed to quantify the discordance between p16 expression and HPV status in oropharyngeal cancer and evaluate its prognostic implications. The results showed that patients with p16-/HPV+ or p16+/HPV- had worse prognosis compared to p16+/HPV+ or p16-/HPV- patients. HPV testing should be mandatory in clinical trials and recommended in patient care settings where HPV status may have an impact.
Article
Oncology
Emmanouil Terzidis, Jeppe Friborg, Ivan R. Vogelius, Giedrius Lelkaitis, Christian von Buchwald, Anders B. Olin, Helle H. Johannesen, Barbara M. Fischer, Irene Wessel, Jacob H. Rasmussen
Summary: This study aimed to evaluate the mismatch between tumor volumes defined from PET/MRI hybrid imaging and pathology, and its potential clinical impact. The results showed a mismatch between imaging and pathological defined tumor volumes in all patients. However, after the addition of a clinical 5 mm margin, the entire pathological volume was fully encompassed in the imaging-defined tumor volume in most patients.
RADIOTHERAPY AND ONCOLOGY
(2023)
Article
Respiratory System
Tobias Todsen, Martin G. Tolsgaard, Thomas Benfield, Fredrik Folke, Kathrine K. Jakobsen, Niels Tobias Gredal, Annette K. Ersboll, Christian von Buchwald, Nikolai Kirkby
Summary: This study compared the detection rate of SARS-CoV-2 using nasopharyngeal swab (NPS), oropharyngeal swab (OPS), and saliva specimen collection. The results showed that OPS had the highest detection rate, while saliva sampling had the lowest. OPS sampling had higher detection rate and lower discomfort compared to NPS. Although saliva sampling had the lowest detection rate, it was the least costly.
Editorial Material
Virology
Tina Dalianis, Christian von Buchwald, Anders Nasman, Stina Syrjanen
Editorial Material
Otorhinolaryngology
Jacob P. S. Nielsen, Christian von Buchwald, Christian Gronhoj
ACTA OTO-LARYNGOLOGICA
(2023)
Review
Otorhinolaryngology
Theresa Emilia Britze, Kathrine Kronberg Jakobsen, Christian Gronhoj, Christian von Buchwald
Summary: This study reviewed the role of biomarkers in liquid biopsies and saliva samples in monitoring salivary gland cancer. Five studies identified potential biomarkers in blood and saliva samples, and concluded that further research is needed before the method can be used for diagnosis.
ACTA OTO-LARYNGOLOGICA
(2023)
Article
Infectious Diseases
Magnus Bock, Johan G. C. Van Hasselt, Franziska Schwartz, Hengzhuang Wang, Niels Hoiby, Kurt Fuursted, Nikolaj Ihlemann, Sabine Gill, Ulrik Christiansen, Niels Eske Bruun, Hanne Elming, Jonas A. Povlsen, Lars Kober, Dan E. Hofsten, Emil L. Fosbol, Mia M. Pries-Heje, Jens Jorgen Christensen, Flemming S. Rosenvinge, Christian Torp-Pedersen, Jannik Helweg-Larsen, Niels Tonder, Kasper Iversen, Henning Bundgaard, Claus Moser
Summary: This study characterized the population pharmacokinetics of linezolid in patients with infective endocarditis and found that there is a substantial interaction between linezolid and rifampicin, which increases the clearance of linezolid. Model-based simulations showed that increasing the linezolid dose can compensate for the interaction without increasing the risk of adverse effects.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Mads Lawaetz, Tina Binderup, Anders Christensen, Karina Juhl, Giedrius Lelkaitis, Eva Lykke, Line Knudsen, Christian von Buchwald, Andreas Kjaer
Summary: This study established oral squamous cell carcinoma (OSCC) PDX models and evaluated the effectiveness of [Cu-64]Cu-uPAR-PET/CT for tumor imaging in these models.
MOLECULAR IMAGING AND BIOLOGY
(2023)
Editorial Material
Oncology
Mario A. Hermsen, Matt Lechner, Leticia Oliveira Ferrer, Annalisa Trama, Patrick Rene Gerhard Eriksen, Eva Martinez-Balibrea, Christian von Buchwald
Summary: Sinonasal cancer is a rare and heterogeneous group of tumors with poor outcomes. Due to their low incidence, collaboration between physicians and researchers is essential for advancement in clinical management and translational research. This report discusses a conference organized by EUSICA and COST Action 'IMMUNO-model' that focused on collaboration, preclinical tumor and immuno models, treatment options, genetic characterization, and cancer registry for sinonasal cancer.
