Article
Biochemistry & Molecular Biology
Aurelie Maisonial-Besset, Tiffany Witkowski, Mercedes Quintana, Sophie Besse, Vincent Gaumet, Axel Cordonnier, Cyrille Alliot, Aurelien Vidal, Caroline Denevault-Sabourin, Sebastien Tarrit, Sophie Levesque, Elisabeth Miot-Noirault, Jean-Michel Chezal
Summary: The development of Cu-64-based immuno-PET radiotracers requires the use of copper-specific bifunctional chelators for stable copper complexes. In this study, the potential of the pentaazamacrocyclic [Cu-64]Cu-15-5 complex for Cu-64-labelling of monoclonal antibodies (mAbs) was investigated. Different BFCs were synthesized and covalent bonds were formed between them and azide-functionalized trastuzumab using strain-promoted azide-alkyne cycloaddition. The NODAGA- and 15-5-mAb conjugates showed excellent radiolabeling yields with Cu-64, and presented higher resistance to transchelation when challenged by EDTA compared to the DOTA derivative.
Article
Medicine, General & Internal
Inki Lee, Min Hwan Kim, Kyongkyu Lee, Keumrok Oh, Hyunwoo Lim, Jae Hun Ahn, Yong Jin Lee, Gi Jeong Cheon, Dae Yoon Chi, Sang Moo Lim
Summary: This study compared the effects of DOTA and NOTA as Cu-64-chelating agents on the pharmacokinetics of newly developed PSMA target compounds.
Article
Pharmacology & Pharmacy
Hailey A. Houson, Volkan Tekin, Wilson Lin, Eduardo Aluicio-Sarduy, Jonathan W. Engle, Suzanne E. Lapi
Summary: The study demonstrates that [Co-55] Co-NOTA-NT-20.3 exhibits superior cell uptake and increasing tumor to blood ratios over time compared to [Ga-68] Ga-NOTA-NT-20.3 and [Cu-64] Cu-NOTA-NT-20.3, indicating its enhanced targeting efficacy for NTSR1.
Article
Biochemical Research Methods
Feng Li, Zihua Zeng, Dale Hamilton, Youli Zu, Zheng Li
Summary: The aptamer radiotracer demonstrates specific binding to tumor cells and can be used for tumor-specific PET imaging by chelating a Cu-64 isotope and DOTA-PEGylated aptamer sequence to target EpCAM. In vivo studies in xenograft mice showed rapid and specific tumor uptake of the aptamer radiotracer, highlighting its potential for accurate tumor staging and therapy evaluation.
BIOCONJUGATE CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Ibolya Kalman-Szabo, Judit P. Szabo, Viktoria Arato, Noemi Denes, Gabor Opposits, Istvan Joszai, Istvan Kertesz, Zita Kepes, Aniko Fekete, Dezso Szikra, Istvan Hajdu, Gyorgy Trencsenyi
Summary: This study investigated the GRPR specificity of the newly synthesized [Sc-44]Sc-NODAGA-AMBA and its potential as a valuable radiotracer in the imaging of GRPR-positive PCa through in vitro and in vivo experiments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Seong-Wook Park, Sei-Yong Jun, Ji-Sun Kim, Yong-Sung Kim
Summary: By engineering a higher affinity EpCAM-targeting cyclic peptide, the peptide-fused antibody showed enhanced cellular internalization and tumor accumulation, leading to more efficient antitumor effects.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Oncology
Vita Golubovskaya, John Sienkiewicz, Jinying Sun, Yanwei Huang, Liang Hu, Hua Zhou, Hizkia Harto, Shirley Xu, Robert Berahovich, Walter Bodmer, Lijun Wu
Summary: Colorectal cancer, a common cancer worldwide, requires novel treatments. This study developed three different designs of EpCAM-CD3 antibodies and tested their efficacy against colorectal tumors. The antibodies showed high efficacy and specificity, and a novel method of delivering bispecific antibodies using mRNA-LNP technology was demonstrated. The data provide a basis for future clinical studies.
Article
Radiology, Nuclear Medicine & Medical Imaging
Marti Boss, Christof Rottenburger, Winfried Brenner, Oliver Blankenstein, Vikas Prasad, Sonal Prasad, Paolo de Coppi, Peter Kuhnen, Mijke Buitinga, Pirjo Nuutila, Timo Otonkoski, Khalid Hussain, Maarten Brom, Annemarie Eek, Jamshed Bomanji, Pratik Shah, Martin Gotthardt
Summary: This study compared the performance of F-18-DOPA PET/CT and Ga-68-NODAGA-exendin-4 PET/CT in the preoperative detection of focal congenital hyperinsulinism (CHI). The results showed that Ga-68-NODAGA-exendin-4 PET/CT had higher sensitivity and better interobserver correlation, as well as better image quality, making it more effective for surgeons in locating the lesions before surgery.
JOURNAL OF NUCLEAR MEDICINE
(2022)
Article
Medicine, General & Internal
Arghavan Jahandideh, Mia Stahle, Jenni Virta, Xiang-Guo Li, Heidi Liljenbaeck, Olli Moisio, Juhani Knuuti, Anne Roivainen, Antti Saraste
Summary: The study demonstrated the potential of using alpha(v)beta(3)-targeting PET imaging with [Ga-68]Ga-NODAGA-RGD to specifically detect myocardial inflammation in a rat model of autoimmune myocarditis. The results showed higher accumulation of the tracer in inflamed myocardium compared to non-inflamed tissue, indicating its efficacy in identifying active inflammatory lesions.
FRONTIERS IN MEDICINE
(2021)
Editorial Material
Radiology, Nuclear Medicine & Medical Imaging
Wei Liu, Xiao Yang, Liming Chen, Yue Chen, Tingting Xu
Summary: A 28-year-old woman presented with a 2-month history of chest wall swelling and pain. Chest CT revealed an irregular soft tissue mass in the chest wall and mediastinum. F-18-FDG PET/CT showed significant FDG uptake in the mass. Subsequently, the patient participated in a Ga-68-DOTA-FAPI-04 PET/CT tumor clinical trial, which also showed elevated tracer uptake in the mass. Biopsy confirmed a diagnosis of myeloid sarcoma.
CLINICAL NUCLEAR MEDICINE
(2023)
Article
Immunology
Roya Mirzaei, Soodabeh Shafiee, Rana Vafaei, Malihe Salehi, Neda Jalili, Zahra Nazerian, Ahad Muhammadnajad, Fatemeh Yadegari, Mohamad Reza Esmailinejad, Leila Farahmand
Summary: Researchers successfully cloned and expressed an anti-EpCAM scFv antibody for breast cancer treatment. The recombinant antibody showed specific binding to malignant breast cancer cells and inhibited their migration and invasion ability. In vivo experiments with mouse models showed significant suppression of tumor growth and reduction in blood supply in response to the recombinant antibody intervention. These findings suggest that the antibody could be a therapeutic tool for reducing invasion and proliferation in malignant breast cancer.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Medicine, General & Internal
Bjarke Follin, Cecilie Hoeeg, Ingrid Hunter, Simon Bentsen, Morten Juhl, Jacob Kildevang Jensen, Tina Binderup, Carsten Haagen Nielsen, Rasmus Sejersten Ripa, Jens Kastrup, Annette Ekblond, Andreas Kjaer
Summary: The aim of this study was to investigate the prognostic value of imaging angiogenesis and macrophage activation via positron emission tomography (PET) in terms of functional improvement after cell therapy. The results showed that rats with improved left ventricular ejection fraction (LVEF) had higher uptake of both [Ga-68]Ga-RGD and [Cu-64]Cu-DOTATATE at follow-up. The uptake of these tracers could predict improvement in LVEF and could be used to improve the monitoring of heart failure patients in treatment.
Article
Radiology, Nuclear Medicine & Medical Imaging
Yan Wang, Chao Wang, Minzhou Huang, Songbing Qin, Jun Zhao, Shibiao Sang, Meng Zheng, Yicong Bian, Chenrong Huang, Hua Zhang, Lingchuan Guo, Jiwei Jiang, Chun Xu, Na Dai, Yushuang Zheng, Jiajun Han, Min Yang, Tao Xu, Liyan Miao
Summary: In this study, a novel probe, (68) Ga-NODAGA-SNA006, was developed and evaluated for instant PET imaging of CD8(+) T cells. The results showed specific uptake of (68) Ga-NODAGA-SNA006 in CD8-rich tissues and CD8(+) tumor lesions, indicating its potential for tracking CD8(+) T cells and monitoring immune responses for individualized cancer immunotherapy.
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2022)
Article
Biotechnology & Applied Microbiology
Jiaying Zhang, Zefang Lin, Xiaojun Zhang, Rong Lin, Mengchao Cui, Weibing Miao, Shaobo Yao
Summary: The injection of Ga-68-DOTA-DiPSMA is safe and associated with low absorbed and effective doses. Ga-68-DOTA-DiPSMA demonstrates favorable kinetics and imaging characteristics, showing potential as an alternative for gallium-68-labeled PSMA clinical PET.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Chunxia Qin, Fuqiang Shao, Yongkang Gai, Qingyao Liu, Weiwei Ruan, Fang Liu, Fan Hu, Xiaoli Lan
Summary: The study evaluated the performance of Ga-68-FAPI PET/MR in diagnosing primary tumors and metastatic lesions in patients with gastric carcinomas, comparing it with F-18-FDG PET/CT. The results showed that Ga-68-FAPI PET/MR outperformed F-18-FDG PET/CT in detecting primary tumors and most metastatic lesions, suggesting its potential as a replacement for F-18-FDG PET/CT.
JOURNAL OF NUCLEAR MEDICINE
(2022)
Review
Pharmacology & Pharmacy
M. J. Fowler, J. D. Cotter, B. E. Knight, E. M. Sevick-Muraca, D. I. Sandberg, R. W. Sirianni
ADVANCED DRUG DELIVERY REVIEWS
(2020)
Article
Computer Science, Interdisciplinary Applications
Banghe Zhu, Sunkuk Kwon, John C. Rasmussen, Maritoni Litorja, Eva M. Sevick-Muraca
IEEE TRANSACTIONS ON MEDICAL IMAGING
(2020)
Article
Surgery
John C. Rasmussen, Banghe Zhu, John R. Morrow, Melissa B. Aldrich, Aaron Sahihi, Stuart A. Harlin, Caroline E. Fife, Thomas F. O'Donnell, Eva M. Sevick-Muraca
Summary: Near-infrared fluorescence lymphatic imaging showed that lymphatic anatomy and contractile function deteriorate with the severity of venous insufficiency in comparison to normal health subjects. Lymphatic abnormalities in venous insufficiency resemble those in early cancer-acquired lymphedema.
JOURNAL OF VASCULAR SURGERY-VENOUS AND LYMPHATIC DISORDERS
(2021)
Review
Oncology
Melissa B. Aldrich, John C. Rasmussen, Caroline E. Fife, Simona F. Shaitelman, Eva M. Sevick-Muraca
Article
Immunology
Alexandre E. Malek, Caroline E. Fife, John C. Rasmussen, Ron J. Karni, John R. Morrow, Audrey Wanger, Eva M. Sevick-Muraca, Luis Ostrosky-Zeichner
Summary: Cutaneous tuberculosis caused by skin inoculation of Mycobacterium tuberculosis is rare but can occur in healthcare settings. This case report describes an unusual case of primary cutaneous tuberculosis of the thumb following a needlestick injury, with progression to necrotic granuloma, lymphatic dysfunction, and axillary web syndrome.
OPEN FORUM INFECTIOUS DISEASES
(2021)
Meeting Abstract
Oncology
Melissa B. Aldrich, Meghan E. McWain, Wenyaw Chan, John C. Rasmussen, Sarah DeSnyder, Eva M. Sevick-Muraca, Simona F. Shaitelman
Meeting Abstract
Oncology
Wintana Balema, Janelle Morton, Richard Larson, Fred C. Velasquez, Eva Sevick-Muraca, Wendy A. Woodward
Meeting Abstract
Oncology
Anna R. Vang, Simona F. Shaitelman, Eva M. Sevick-Muraca, Chan Wen-yaw, John C. Rasmussen, Sarah D. DeSnyder, Melissa B. Aldrich
Editorial Material
Nanoscience & Nanotechnology
Eva M. Sevick-Muraca
NATURE NANOTECHNOLOGY
(2022)
Article
Oncology
Carolina Mantilla-Rojas, Fred C. Velasquez, Janelle E. Morton, Leticia C. Clemente, Edwin R. Parra, Carlos Torres-Cabala, Eva M. Sevick-Muraca
Summary: This study demonstrates that combining vaccination and checkpoint blockade immunotherapy delivered through the lymphatics can improve anti-tumor responses in non-immunogenic melanoma models. This method may potentially benefit more patients by directing immune responses within the regional lymph node environment.
Article
Oncology
Melissa B. Aldrich, John C. Rasmussen, Sarah M. DeSnyder, Wendy A. Woodward, Wenyaw Chan, Eva M. Sevick-Muraca, Elizabeth A. Mittendorf, Benjamin D. Smith, Michael C. Stauder, Eric A. Strom, George H. Perkins, Karen E. Hoffman, Melissa P. Mitchell, Carlos H. Barcenas, Lynn E. Isales, Simona F. Shaitelman
Summary: This study investigated the use of near-infrared fluorescence lymphatic imaging (NIRF-LI) surveillance to predict early onset of peripheral lymphatic dysfunction as a predictor of breast cancer-related lymphedema (BCRL). The results showed that dermal backflow can predict the development of BCRL months before arm swelling occurs, enabling early treatment to prevent edema and irreversible tissue changes.
BREAST CANCER RESEARCH AND TREATMENT
(2022)
Article
Oncology
Anna R. Vang, Simona F. Shaitelman, John C. Rasmussen, Wenyaw Chan, Eva M. Sevick-Muraca, Melissa B. Aldrich
Summary: Breast cancer-related lymphedema (BCRL) occurs in approximately 40% of patients after axillary lymph node dissection (ALND), radiation therapy (RT), or chemotherapy. First-line palliative treatment involves the use of compression garments and specialized massage. Reparative microsurgeries are a second-line treatment, but both compression and surgical therapy are most effective in the early stages of LE development. Identifying patients at the highest risk for BCRL would allow for earlier and more effective treatment.
Article
Computer Science, Interdisciplinary Applications
Banghe Zhu, Eva M. Sevick-Muraca, Ryan D. Nguyen, Manish N. Shah
IEEE TRANSACTIONS ON MEDICAL IMAGING
(2020)
Article
Medicine, Research & Experimental
Kay T. Pham, Duraisamy Balaguru, Varaha S. Tammisetti, Carlos J. Guevara, John C. Rasmussen, Rodrick C. Zvavanjanja, Robert Hanfland, Eva M. Sevick-Muraca, Melissa B. Aldrich
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2020)
Article
Physiology
John C. Rasmussen, Sunkuk Kwon, Amanda Pinal, Alexander Bareis, Fred C. Velasquez, Christopher F. Janssen, John R. Morrow, Caroline E. Fife, Ron J. Karni, Eva M. Sevick-Muraca
PHYSIOLOGICAL REPORTS
(2020)