期刊
JOURNAL OF CONTROLLED RELEASE
卷 196, 期 -, 页码 132-138出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jconrel.2014.10.006
关键词
Transfection; Gene silencing; Non-viral vectors; siRNA; Polyplexes; Microcapsules
资金
- LOEWE (grant Synbiochem)
The efficiency of siRNA delivery is demonstrated to be improved by encapsulating the siRNA within a non-viral carrier based on layer-by-layer assembly of oppositely charged biodegradable and biocompatible polyelectrolytes. In comparison to other non-viral delivery vehicles such as polycation-based complexes, a smaller amount of siRNA was necessary to produce in vitro gene silencing as early as 20-30 h after incubation. Colloidal carriers based on assembled biodegradable polyelectrolytes offer several advantages, such as efficient intracellular delivery after endocytosis followed by release to the cytosol, as well as protection of the siRNA, which is crucial for its therapeutic activity. (C) 2014 Elsevier B.V. All rights reserved.
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