期刊
JOURNAL OF CONTROLLED RELEASE
卷 149, 期 1, 页码 15-20出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jconrel.2010.05.012
关键词
Transcutaneous immunization; Vaccine; Hydrogel patch; Tetanus; Diphtheria
资金
- Fundamental Studies in Health Sciences of the National Institute of Biomedical Innovation (NIBIO)
- Mochida Memorial Foundation for Medical and Pharmaceutical Research
- Tokyo Biochemical Research Foundation
Transcutaneous immunization (TCI) targeting the Langerhans cells (LCs) of the epidermal layer is a promising needle-free, easy-to-use, and non-invasive vaccination method. We developed a hydrogel patch formulation to promote the penetration of antigenic proteins into the stratum corneum. Here, we investigated the characteristics of the immune responses induced by this vaccination method and the vaccine efficacy of TCI using a hydrogel patch containing tetanus and diphtheria toxoids. Our TCI system induced toxoid-specific IgG production in an antigen dose-, patch area-, and application period-dependent manner. Moreover, IgG subclass analysis indicated that our TCI predominantly elicited a Th2-type immune response rather than a Th1-type immune response. Importantly, our TCI system induced antigen-specific immune memory based on the booster effect and showed potent efficacy, comparable to that of subcutaneous immunization in toxin-challenge experiments. On the basis of these results, we are now performing translational research to apply TCI for tetanus and diphtheria. (C) 2010 Elsevier B.V. All rights reserved.
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