Article
Medicine, Research & Experimental
Markus Vogt, Benjamin Schulz, Ahmed Wagdi, Jan Lebert, Gijsbert J. van Belle, Jan Christoph, Tobias Bruegmann, Robert Patejdl
Summary: This study demonstrates the direct optogenetic stimulation of smooth muscle cells to control gastric contractility, showing promising potential for restoring motility in gastroparesis in the future.
Article
Biochemistry & Molecular Biology
Yun-Mi Jeong, Tae-Ik Choi, Kyu-Seok Hwang, Jeong-Soo Lee, Robert Gerlai, Cheol-Hee Kim
Summary: Recent advancements in optogenetics have allowed researchers to manipulate the olfactory system in zebrafish, revealing that light stimulation can activate olfactory neurons. Zebrafish larvae exhibit a preference for light over aversive odors when presented with conflicting sensory cues, showcasing the importance of olfaction in decision-making processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Gur Pyari, Himanshu Bansal, Sukhdev Roy
Summary: The main challenge in cardiac optogenetics is to achieve low-power, high-fidelity deep excitation of cells. Recent research has discovered new optogenetic proteins, ChRmine and bReaChES, which can enable safe sustained excitation of deeply situated cardiac cells, providing important implications for cardiac rhythm regulation and disease treatment.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Article
Chemistry, Analytical
Saeyeong Jeon, Youjin Lee, Daeho Ryu, Yoon Kyung Cho, Yena Lee, Sang Beom Jun, Chang-Hyeon Ji
Summary: Optogenetics has become crucial in neuroscience research due to its cell-type-specific neuromodulation feature. Technological advances in light delivery devices have allowed for the combination of optogenetics and electrophysiology, as demonstrated by a novel optrode array design for optical modulation and electrophysiological recording. In vivo experiments with transgenic mice expressing channelrhodopsin have confirmed the effectiveness of optical activation and neural recording.
Review
Biochemistry & Molecular Biology
Kotaro Yamashiro, Yuji Ikegaya, Nobuyoshi Matsumoto
Summary: The complexity of brain functions requires precise manipulation of specific neuronal subtypes, which can be achieved through the combination of in utero electroporation and optogenetics. This method provides high spatiotemporal resolution control to study the functions and characteristics of specific regions, layers, and cell types.
Article
Biochemical Research Methods
Sebastian Junge, Felix Schmieder, Philipp Sasse, Jurgen Czarske, Maria Leilani Torres-Mapa, Alexander Heisterkamp
Summary: Controlling and reading the electrical activity in a cardiac syncytium using optical approaches can enhance our understanding of cardiac electrophysiology. In this study, we demonstrate the precise optogenetic stimulation of cardiomyocytes using light foci generated with a spatial light modulator. Additionally, we show that probing the cardiac cells with multiple light foci enhances the excitability of the cardiac network.
JOURNAL OF BIOPHOTONICS
(2022)
Article
Cell Biology
Yi-Shyun Lai, Ya-Han Chang, Yong-Yi Chen, Jixuan Xu, Chi-Sian Yu, Su-Jing Chang, Pai-Sheng Chen, Shaw-Jenq Tsai, Wen-Tai Chiu
Summary: This study demonstrates the integral role of Ca2+ signals in cell biology, and optogenetic technology can be used to modulate Ca2+ waves and activate specific signaling pathways to explore the regulation of cell migration.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biophysics
Max Eickenscheidt, Thoralf Herrmann, Marius Weisshap, Annette Mittnacht, Linda Rudmann, Gunther Zeck, Thomas Stieglitz
Summary: Optogenetics provides a new method for cell-specific activation, which is more precise compared to electrical stimulation. This study presents the design of a miniaturized optoelectronic device that allows simultaneous optical and electrical stimulation at the same spot.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemistry & Molecular Biology
Phillip Wright, Jessica Rodgers, Jonathan Wynne, Paul N. Bishop, Robert J. Lucas, Nina Milosavljevic
Summary: The study evaluated the safety of viral gene delivery of human rod opsin and two channelrhodopsins in ON bipolar cells of the mouse retina. The results showed that delivery of these opsins did not cause significant changes in retinal health or impact the survival of targeted cells. Additionally, there was no increase in retinal inflammation in treated mice compared to controls.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Lakshmini Balachandar, Diana Borrego, Jorge Riera Diaz
Summary: Optogenetics is a modern technique that can be used for gene delivery in non-neuronal cell types, including astrocytes. In the rat brain, AAV serotype 8 has been shown to exhibit the most promising transduction patterns in astrocytes, which is important for the future application of optogenetics.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Neurosciences
Savitha Sridharan, Marta A. Gajowa, Mora B. Ogando, Uday K. Jagadisan, Lamiae Abdeladim, Masato Sadahiro, Hayley A. Bounds, William D. Hendricks, Toby S. Turney, Ian Tayler, Karthika Gopakumar, Ian Anton Oldenburg, Stephen G. Brohawn, Hillel Adesnik
Summary: Structure-guided mutagenesis has been used to engineer new optogenetic tools with high potency and fast kinetics, enabling precise control of population neural activity at a large scale, and temporally and spatially. These new tools have been extensively benchmarked and characterized, providing a resource for patterned optogenetics experiments. Furthermore, when combined with optimized procedures for holographic photostimulation, these new tools allow for the coactivation of hundreds of spatially defined neurons simultaneously with a single hologram.
Article
Optics
Himanshu Bansal, Gur Pyari, Sukhdev Roy
Summary: The fundamental process of information processing and memory formation in the brain is associated with complex neuron firing patterns, which can be precisely manipulated using light pulses in optogenetics. This study presents a detailed theoretical analysis of the temporal shaping of light pulses and demonstrates that non-square waveforms generate more naturalistic spiking patterns compared to traditional square pulses. These findings contribute to the development of new optogenetic strategies for simulating and manipulating neural activity patterns in the brain.
Article
Neurosciences
Luis E. Martinetti, Kelly E. Bonekamp, Dawn M. Autio, Hye-Hyun Kim, Shane R. Crandall
Summary: Short-term plasticity plays a crucial role in regulating the strength of central synapses based on previous activity. This study investigates the connections between mouse primary somatosensory and motor cortex using an optogenetic approach. It reveals that corticocortical synapses exhibit strong short-term facilitation, leading to more sustained responses compared to local intracortical and thalamocortical connections. The properties of these connections suggest their specialization in conveying information over extended periods.
Article
Neurosciences
Daniel J. Rindner, Gyorgy Lur
Summary: The ability to control synaptic communication is essential in modern neuroscience. Recent advancements in protein engineering and screening have greatly expanded the optogenetic toolkit, allowing for multicolor approaches in studying neural circuits. However, the scarcity of opsins with truly discrete spectra poses challenges in avoiding unintended cross-activation of optogenetic tools (crosstalk).
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Cell Biology
Liying Zhang, Kaituo Wang, Shuo Ning, Per Amstrup Pedersen, Annette Susanne Duelli, Pontus Emanuel Gourdon
Summary: In this study, the production and purification procedure of the D156C form of ChR2, which exhibits prolonged channel opening, was reported. Furthermore, conditions for growing ChR2 crystals that scatter X-rays to 6 angstrom were developed. The results provide a foundation for future structural studies of ChR2 in different states.
Article
Neurosciences
Nada Yousif, Richard Z. Fu, Bilal Abou-El-Ela Bourquin, Vamsee Bhrugubanda, Simon R. Schultz, Barry M. Seemungal
JOURNAL OF NEUROSCIENCE
(2016)
Article
Mathematical & Computational Biology
Benjamin D. Evans, Sarah Jarvis, Simon R. Schultz, Konstantin Nikolic
FRONTIERS IN NEUROINFORMATICS
(2016)
Article
Neurosciences
Kit Cheung, Simon R. Schultz, Wayne Luk
FRONTIERS IN NEUROSCIENCE
(2016)
Article
Computer Science, Artificial Intelligence
Romain D. Caze, Sarah Jarvis, Amanda J. Foust, Simon R. Schultz
NEURAL COMPUTATION
(2017)
Article
Neurosciences
Luca A. Annecchino, Alexander R. Morris, Caroline S. Copeland, Oshiorenoya E. Agabi, Paul Chadderton, Simon R. Schultz
Article
Engineering, Biomedical
Renaud Schuck, Mary Ann Go, Stefania Garasto, Stephanie Reynolds, Pier Luigi Dragotti, Simon R. Schultz
JOURNAL OF NEURAL ENGINEERING
(2018)
Article
Biochemical Research Methods
Sarah Jarvis, Konstantin Nikolic, Simon R. Schultz
PLOS COMPUTATIONAL BIOLOGY
(2018)
Article
Engineering, Biomedical
Jon Onativia, Simon R. Schultz, Pier Luigi Dragotti
JOURNAL OF NEURAL ENGINEERING
(2013)
Article
Physics, Applied
Navjeevan S. Soor, Peter Quicke, Carmel L. Howe, Kuin T. Pang, Mark A. A. Neil, Simon R. Schultz, Amanda J. Foust
JOURNAL OF PHYSICS D-APPLIED PHYSICS
(2019)
Article
Engineering, Biomedical
Carl H. Lubba, Zhonghua Ouyang, Nick S. Jones, Tim M. Bruns, Simon R. Schultz
Summary: This study characterizes the encoding of bladder pressure by sensory fibers and explores a decoding paradigm based on information theory. The research demonstrates that different types of bladder neurons offer reliability and high information rates through redundancy and semi-independence.
JOURNAL OF NEURAL ENGINEERING
(2021)
Review
Mathematical & Computational Biology
Rufus Mitchell-Heggs, Selgfred Prado, Giuseppe P. Gava, Mary Ann Go, Simon R. Schultz
Summary: Recent developments in experimental neuroscience have enabled the simultaneous recording of activity from thousands of neurons. Neural manifold learning is a popular approach that takes advantage of the low-dimensional dynamics of neural activity to uncover neural circuits' links to cognitive function. This paper reviews various linear and non-linear approaches to neural manifold learning, compares their advantages and disadvantages in analyzing neural data, and applies them to different datasets to demonstrate their applicability. The results show that both linear and non-linear methods produce similar results in many cases, with non-linear methods being more effective in cases with higher behavioral complexity. The application of these methods to a mouse model of Alzheimer's Disease suggests their potential in understanding the circuit-level consequences of neurological disorders.
JOURNAL OF COMPUTATIONAL NEUROSCIENCE
(2023)
Article
Neurosciences
Stephanie Reynolds, Therese Abrahamsson, Renaud Schuck, P. Jesper Sjostrom, Simon R. Schultz, Pier Luigi Dragotti
Proceedings Paper
Engineering, Biomedical
Stephanie Reynolds, Caroline S. Copeland, Simon R. Schultz, Pier Luigi Dragotti
2016 IEEE 13TH INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING (ISBI)
(2016)
Review
Neurosciences
Sarah Jarvis, Simon R. Schultz
FRONTIERS IN SYSTEMS NEUROSCIENCE
(2015)