期刊
JOURNAL OF COMPARATIVE NEUROLOGY
卷 515, 期 4, 页码 397-408出版社
WILEY
DOI: 10.1002/cne.22065
关键词
brainstem; cholinergic neurons; in situ hybridization; stereology; topography; basal ganglia
资金
- Parkinson's Disease Society of the United Kingdom [4049]
- Medical Research Council U.K. [G0400313]
- University Research Fellowship from The Royal Society
- MRC [MC_U138164490, MC_U120085816, G0400313] Funding Source: UKRI
- Medical Research Council [MC_U138164490, G0400313, MC_U120085816] Funding Source: researchfish
gamma-Aminobutyric acid (GABA)ergic neurons are widely distributed in brainstem structures involved in the regulation of the sleep-wake cycle, locomotion, and attention. These brainstem structures include the pedunculopontine nucleus (PPN), which is traditionally characterized by its population of cholinergic neurons that have local and wide-ranging connections. The functional heterogeneity of the PPN is partially explained by the topographic distribution of cholinergic neurons, but such heterogeneity might also arise from the organization of other neuronal populations within the PPN. To understand whether a topographical organization is also maintained by GABAergic neurons, we labeled these neurons by in situ hybridization for glutamic acid decarboxylase mRNA combined with immunohistochemistry for choline acetyltransferase to reveal cholinergic neurons. We analyzed their distribution within the PPN by using a method to quantify regional differences based on stereological cell counts. We show that GABAergic neurons of the rat PPN have a rostrocaudal gradient that is opposite to that of cholinergic neurons. Indeed, GABAergic neurons are predominantly concentrated in the rostral PPN; in addition, they form, along with cholinergic neurons, a small, high-density cluster in the most caudal portion of the nucleus. Thus, we provide evidence of heterogeneity in the distribution of different neuronal populations in the PPN and show that GABAergic and cholinergic neurons define neurochemically distinct areas. Our data suggest that the PPN is neurochemically segregated, and such differences define functional territories. J. Comp. Neurol. 515:397-408, 2009. (C) 2009 Wiley-Liss, Inc.
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