Article
Neurosciences
Hourong Wang, Zhifeng Liu, Tianyuan Yu, Yingqi Zhang, Yajing Xu, Yi Jiao, Qian Guan, Di Liu
Summary: This study analyzed the changes in gene expression in the dorsal root ganglia (DRG) and spinal dorsal horn (SDH) after tuina treatment and investigated the immediate analgesic mechanism of tuina. The results showed that tuina improved nociceptive behavior in rats with minor chronic constriction injury (CCI), especially in thermal hyperalgesia. Additionally, analysis of differentially expressed genes (DEGs) revealed that the immediate analgesic mechanism of tuina is associated with the calcium signaling pathway, thermogenesis, and regulation of lipolysis in adipocytes.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Benjamin M. Zemel, Lianteng Zhi, Eric V. Brown, Stephen R. Tymanskyj, Qiansheng Liang, Manuel Covarrubias
Summary: The study suggests that a physical interaction between Kv3.4 channel and functional PKC epsilon primarily determines its stability and localization in DRG neurons, without significantly affecting its gating.
Article
Neurosciences
Laszlo Ducza, Andrea Gajtko, Krisztina Hegedus, Erzsebet Bakk, Greta Kis, Botond Gaal, Roland Takacs, Peter Szucs, Klara Matesz, Krisztina Hollo
Summary: This study provides new evidence for the involvement of neuronal and glial P2X4 receptors in the development of inflammatory pain.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2023)
Article
Computer Science, Interdisciplinary Applications
Zhonghua Ouyang, Nikolas Barrera, Zachariah J. Sperry, Elizabeth C. Bottorff, Katie C. Bittner, Lance Zirpel, Tim M. Bruns
Summary: This study aims to improve open-loop sacral neuromodulation therapy by developing a conditional stimulation paradigm using neural recordings as sensory feedback. The results show that closed-loop stimulation increases bladder capacity and reduces stimulation time. It is suggested that the mechanism of sacral neuromodulation may involve a reduction in bladder sensory neuron activity. Real-time validation during behavioral studies is necessary before clinical translation of closed-loop sacral neuromodulation.
MEDICAL & BIOLOGICAL ENGINEERING & COMPUTING
(2022)
Article
Engineering, Biomedical
Carl H. Lubba, Zhonghua Ouyang, Nick S. Jones, Tim M. Bruns, Simon R. Schultz
Summary: This study characterizes the encoding of bladder pressure by sensory fibers and explores a decoding paradigm based on information theory. The research demonstrates that different types of bladder neurons offer reliability and high information rates through redundancy and semi-independence.
JOURNAL OF NEURAL ENGINEERING
(2021)
Article
Multidisciplinary Sciences
Sara Caxaria, Sabah Bharde, Alice M. Fuller, Romy Evans, Bethan Thomas, Petek Celik, Francesco Dell'Accio, Simon Yona, Derek Gilroy, Mathieu-Benoit Voisin, John N. Wood, Shafaq Sikandar
Summary: We demonstrated the role of neutrophils in fibromyalgia by using a back-translational approach in mice. Adoptive transfer of neutrophils from mice with chronic widespread pain or patients with fibromyalgia can induce mechanical pain in recipient mice, sensitize spinal cord neurons, and cause changes in the expression of neutrophil proteins that lead to neutrophil infiltration into dorsal root ganglia. These findings suggest an immunological basis for chronic widespread pain in fibromyalgia mediated by neutrophils.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Cristina Fraquelli, Jasmine Hauzinger, Christian Humpel, Maria Nolano, Vincenzo Provitera, Vinay Kumar Sharma, Peng Loh, Zenon Pidsudko, Georgios Blatsios, Josef Troger
Summary: This study investigated the presence and source of serpinins in the skin, and found that serpinin was present in the dorsal root ganglia and sensory nerves of rats, with colocalization with substance P. Although predominantly found in its free form in the skin, the serpinins may have functional significance in the sensory innervation of the skin.
Article
Biochemistry & Molecular Biology
Ruirui Lu, Katharina Metzner, Fangyuan Zhou, Cathrin Flauaus, Annika Balzulat, Patrick Engel, Jonas Petersen, Rebekka Ehinger, Anne Bausch, Peter Ruth, Robert Lukowski, Achim Schmidtko
Summary: The sodium-activated potassium channel Slack plays a role in sensory neurons and is involved in mediating sodium-activated potassium current (I-KNa) and modulating neuronal activity. Studies found that Slack activity is reduced in sensory neurons after peripheral nerve injury, contributing to neuropathic pain hypersensitivity, and that it is associated with ATP-sensitive P2X3 receptors. Experiments showed that Slack-mediated I-KNa can be bidirectionally modulated in response to P2X3 activation, suggesting a potential novel strategy for treating neuropathic pain.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Pawel Janikiewicz, Barbara Wasilewska, Urszula Mazur, Amelia Franke-Radowiecka, Mariusz Majewski, Agnieszka Bossowska
Summary: The study revealed that GUA significantly influences the sensory innervation of the porcine urinary bladder, potentially serving as a tool for investigating the plasticity of bladder innervation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Anesthesiology
Xiao-Long Zhang, Jin-Jun Zhang, Zi-Hang Chen, Kai-Bin Yang, Xi Zhang, Yi-Bin Xiao, Yi Lei, Xian-Ying Cao, Man-Xiu Xie
Summary: Research has shown that the SIRT1 protein plays a role in the different pain vulnerability of adults and minors. The interaction between SIRT1 and the ClC-3 channel contributes to the pain vulnerability difference between adults and minors. Additionally, there is a negative correlation between serum SIRT1 levels and pain scores in chronic pain patients.
Article
Medicine, Research & Experimental
Ying Zhao, Qiang Wang, Chen Xie, Yuling Cai, Xue Chen, Yuhui Hou, Liu He, Jianping Li, Min Yao, Shuangxi Chen, Wutian Wu, Xiaojia Chen, An Hong
Summary: This study developed a novel low-molecular-weight peptide agonist CH02 targeting FGFR, which effectively promoted nerve regeneration, enhanced axon growth of sensory neurons, and robustly improved nerve regeneration and sensory-motor behavioral recovery in a rat spinal dorsal root injury model.
Article
Neurosciences
Javier Lucas-Romero, Ivan Rivera-Arconada, Jose. A. A. Lopez-Garcia
Summary: In this study, the collective behavior of dorsal horn neurons and its relation to backfiring of primary afferents were investigated, along with the effects of peripheral inflammation. The results showed that population bursts of action potentials from neurons presynaptic to the afferents are likely to control their excitability, and peripheral inflammation may enhance the synchrony of these neurons, contributing to central sensitization.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Biochemical Research Methods
Aseer Intisar, Seung Joon Lee, Yu-Gyeong Kim, Woon-Hae Kim, Hyun Young Shin, Min Young Kim, Jong Man Kim, Jungmin Lee, Yun Jeoung Mo, Yu Seon Kim, Seung-Hoon Kim, Yun-Il Lee, Minseok S. Kim
Summary: This study introduces a rapid method for enriching neurons from the mouse embryonic dorsal root ganglion using fully-automated continuous centrifugal microfluidics, achieving effective isolation within 13 minutes. The purified neurons showed improved viability and purity compared to conventional chemical purification methods, highlighting the potential of this automated system to advance neurological research.
Article
Cell Biology
Fangyuan Zhou, Katharina Metzner, Patrick Engel, Annika Balzulat, Marco Sisignano, Peter Ruth, Robert Lukowski, Achim Schmidtko, Ruirui Lu
Summary: The study reveals that Slack serves as a modulator of TRPA1-mediated activation of sensory neurons, but not TRPV1-mediated activation.
Article
Biochemical Research Methods
Cristina Rodriguez, Anderson Chen, Jose A. Rivera, Manuel A. Mohr, Yajie Liang, Ryan G. Natan, Wenzhi Sun, Daniel E. Milkie, Thomas G. Bifano, Xiaoke Chen, Na Ji
Summary: The development of a compact adaptive optics module has enabled correction of tissue-induced aberrations in two-photon and three-photon fluorescence microscopes, allowing high-resolution imaging of synaptic structures in deep regions of the mouse brain and spinal cord, as well as somatosensory-evoked calcium responses. This advancement has facilitated structural and functional imaging deep within living organisms such as mice and zebrafish larvae.