Article
Biochemistry & Molecular Biology
Anjana, Shristhi Rawat, Saswata Goswami
Summary: Polyhydroxybutyrate (PHB) was produced by the halophilic bacteria Bacillus pseudomycoides SAS-B1, which was identified through genome sequencing. The study also revealed genes related to salt tolerance and the increased PHB yield under high salinity conditions. Characterization techniques such as FTIR, NMR, and GC-MS confirmed the molecular structure and composition of PHB. TGA and XRD analysis demonstrated the thermal stability and crystallinity of PHB.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biotechnology & Applied Microbiology
Huixin Li, Chongyang Ai, Xixi Zhao, Bing Pang, Xiaoguang Xu, Wanqin Wu, Guanwen Liu, Chunmei Jiang, Zhongli Pan, Junling Shi
Summary: By isolating wild bacteria from sewage sludge that can remove copper ions in water, the most efficient strain Bacillus pseudomycoides C6 was identified. The properties of C6 in copper ion removal, including reaction conditions and application in oat planting, were investigated. The results showed that C6 biomass could significantly reduce copper content in oat seedlings grown in copper ion contaminated water.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Microbiology
Jing Zhao, Zhijun Zhou, Xuefei Bai, Dai Zhang, Likui Zhang, Jinhui Wang, Beibei Wu, Jiehua Zhu, Zhihui Yang
Summary: This study demonstrates the use of bacteriocin for the control of potato common scab, providing theoretical support for disease control.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Jingqi Chen, Auke J. van Heel, Oscar P. Kuipers
Summary: A membrane-associated lanthipeptide synthetase complex was studied in rod-shaped cells, revealing the spatial distribution and dynamics of NisT, NisB, and NisC along the cell periphery, with highly colocalized NisBTC proteins. Isolation of the biosynthetic complex corroborated that the bright foci were sites for nisin maturation and transportation.
Article
Biotechnology & Applied Microbiology
Michelle M. Gerst, Arpad Somogyi, Xu Yang, Ahmed E. Yousef
Summary: The study successfully detected and characterized a novel lantibiotic from Bacillus spp. using a multifaceted analytical approach, revealing high antimicrobial activity against Gram-positive bacteria. The discovered lantibiotic, Amyloliquecidin GF610, shows potential for applications in food, agricultural, or medical fields, and the analytical approach utilized may aid in future discoveries of challenging compounds like two-component lantibiotics.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ian R. Bothwell, Tania Caetano, Raymond Sarksian, Sonia Mendo, Wilfred A. van der Donk
Summary: Lanthipeptides are a type of ribosomally synthesized and post-translationally modified peptide natural products characterized by lanthionine and methyllanthionine crosslinked amino acids. While most lanthipeptides are identified from Gram-positive bacteria, this study reports the heterologous expression and modification in Escherichia coli of two lanthipeptides from the Gram-negative Bacteroidetes Pedobacter lusitanus NL19, showing novel structural features and unusual stereochemical configuration.
ACS CHEMICAL BIOLOGY
(2021)
Article
Agronomy
Magdalena M. Knezevic, Olivera S. Stajkovic-Srbinovic, Maibassova Assel, Marija D. Milic, Katarina R. Mihajlovski, Dusica Delic, Aneta Buntic
Summary: The study demonstrated that the BM1 strain of Bacillus pseudomycoides has the potential to inhibit fungal growth significantly, while also producing phytohormones, siderophores, and other substances beneficial for plant growth. This strain showed promising results in reducing fungal infections and improving alfalfa seed germination under stress conditions.
Article
Microbiology
Zuzana Rosenbergova, Thomas F. Oftedal, Kirill V. Ovchinnikov, Thasanth Thiyagarajah, Martin Rebros, Dzung B. Diep
Summary: Infections caused by multiresistant pathogens have become a major problem in both human and veterinary medicine. A novel two-peptide lantibiotic with broad-spectrum antimicrobial activity against multiresistant Enterococcus faecium has been isolated from Vagococcus fluvialis, offering potential therapeutic options.
MICROBIOLOGY SPECTRUM
(2022)
Article
Environmental Sciences
Rohit Sharma, Teenu Jasrotia, Ahmad Umar, Monu Sharma, Sonu Sharma, Rajeev Kumar, Abdulrab Ahmed M. Alkhanjaf, Rajeev Vats, Vikas Beniwal, Raman Kumar, Joginder Singh
Summary: Here we report a bacteria-based strategy for remediating heavy metals in waterbodies, which is efficient, reasonable, benign, and promising. The study involves isolating, screening, and characterizing heavy metal resistive bacteria, and conducting batch studies to examine the bioremoval results under different conditions. Mechanistic actions are analyzed using complementary techniques such as SEM and FTIR. The results demonstrate the effectiveness of the isolated bacterial strains in removing heavy metals in simulated water samples, showing great potential for industrial applications.
ENVIRONMENTAL RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Junhua Xu, Daobin Liu, Carmen Lee, Pierre Feydi, Marlene Chapuis, Jing Yu, Emmanuel Billy, Qingyu Yan, Jean-Christophe P. Gabriel
Summary: To develop efficient oxygen evolution reaction (OER) catalysts, this study focuses on upcycling Class II BaTiO3 Multilayer Ceramic Capacitors to recover metal ions and synthesize high potential catalysts NiFe hydroxide and NiCu hydroxide, which have demonstrated faster OER kinetics than commercial c-RuO2.
Article
Chemistry, Multidisciplinary
Ane Valera, Shan Wang, Reuben Carr, Laurent Trembleau, Hai Deng
Summary: The biochemical characterization of a new KARI (MtKARI-II) from a Mycobacterium tuberculosis variant with a similar kinetic profile to class I KARIs is reported here. Phylogenetic analysis suggests that MtKARI-II is clustered into a class II KARI superfamily.
Article
Agricultural Engineering
Xuyang Zhu, Kang Zhang, Hui Luo, Jing Wu
Summary: The bottleneck of recombinant protein production in microbial cell factories is sometimes caused by limited manipulable targets and a lack of gene annotation related to protein expression. In this study, the novel functions of PonA in Bacillus subtilis recombinant protein expression were described, and the mechanism of its chaperone activity was analyzed. Overexpression of PonA led to a significant increase in the expression of hyperthermophilic amylase in shake flasks and fed-batch processes. Increased cell diameter and reinforced cell walls were observed in PonA-overexpressing strains. The FN3 structural domain and the natural dimeric structure of PonA may play critical roles in its chaperone activity. These findings suggest that PonA can be an effective target for modifying the expression of recombinant proteins in B. subtilis.
BIORESOURCE TECHNOLOGY
(2023)
Article
Infectious Diseases
Jerzy Karczewski, Christine M. Brown, Yukari Maezato, Stephen P. Krasucki, Stephen J. Streatfield
Summary: CMB001 maintained stable antibacterial activity in the presence of blood or plasma, efficiently killed S. aureus within the biofilm, and showed equivalent efficacy to vancomycin in treating MRSA infection in neutropenic mice. Further evaluation of its efficacy against other pathogens and expansion of its pharmacokinetic/pharmacodynamic parameters and safety profile are warranted.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Biotechnology & Applied Microbiology
Kajal Chakraborty, Vinaya Kizhakkepatt Kizhakkekalam, Minju Joy, Rekha Devi Chakraborty
Summary: The study identified broad-spectrum bactericidal activity of bacillibactins against multidrug-resistant pathogens. Analysis of gene clusters in B. amyloliquefaciens MTCC 12,713 revealed a correlation between higher electronic values and antibacterial properties.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Mathematics, Applied
Jehan A. Al-Bar, T. Asir, K. Mano, Wafaa M. Fakieh
Summary: In this second part of a series of papers, we study the crosscap two embedding of a class of multipartite graphs and classify lattices with specific conditions, identifying classes of multipartite graphs embedded in the Klein bottle.