Article
Oncology
Lucas Geyer, Thibaut Wolf, Marie-Pierre Chenard, Helene Cebula, Roland Schott, Georges Noel, Eric Guerin, Erwan Pencreach, Damien Reita, Natacha Entz-Werle, Benoit Lhermitte
Summary: The 2021 WHO classification of central nervous system tumors is a histomolecular classification system that incorporates molecular data to improve patient prognoses and treatments. CDKN2A homozygous deletion is associated with poor prognosis in various types of gliomas, and its detection requires complex techniques. This study investigates the clinical significance of p16 immunohistochemical expression in gliomas and its correlation with CDKN2A homozygous deletion.
Article
Oncology
Wei Zhang, Haidong Wang, Huadi Wang, Chuchu Xu, Rongjie Zhao, Junlin Yao, Chongya Zhai, Weidong Han, Hongming Pan, Jin Sheng
Summary: Colorectal cancer is a common cancer with poor prognosis and high recurrence and metastasis rates. DPP7 is found to be significantly overexpressed in colorectal cancer samples compared to non-tumor tissues, and its increased expression is correlated with higher cancer stage and shorter overall survival. Functional annotations suggest that DPP7 is involved in neuroactive ligand-receptor interaction and olfactory transduction signaling. Therefore, DPP7 is a promising diagnostic and prognostic biomarker as well as a potential therapeutic target for colorectal cancer.
Article
Oncology
Anna Faldt Beding, Peter Larsson, Khalil Helou, Zakaria Einbeigi, Toshima Z. Parris
Summary: Our study suggests that BIRC5 overexpression is associated with the initiation and progression of several cancer types, and therefore holds promise as a prognostic biomarker.
Article
Oncology
Qiangan Jing, Chen Yuan, Chaoting Zhou, Weidong Jin, Aiwei Wang, Yanfang Wu, Wenzhong Shang, Guibing Zhang, Xia Ke, Jing Du, Yanchun Li, Fangchun Shao
Summary: CLEC1B, encoding the CLEC-2 protein, is involved in platelet activation, angiogenesis, and immune and inflammatory responses. This study found that CLEC1B is downregulated in hepatocellular carcinoma (HCC) and has a good clinical prognostic value. CLEC1B expression is closely associated with immune cell infiltration and correlates with a variety of immunomodulators in the HCC tumor microenvironment. Furthermore, CLEC1B and its related genes or interacting proteins are implicated in multiple immune-related processes and signaling pathways. Overexpression of CLEC1B significantly influences the treatment effects of sorafenib on HCC cells.
CANCER CELL INTERNATIONAL
(2023)
Article
Oncology
Jan Philipp Kuehn, Wendelin Schmid, Sandrina Koerner, Florian Bochen, Silke Wemmert, Hugo Rimbach, Sigrun Smola, Julia Caroline Radosa, Mathias Wagner, Luc G. T. Morris, Victoria Bozzato, Alessandro Bozzato, Bernhard Schick, Maximilian Linxweiler
Summary: The incidence of human papillomavirus (HPV)-related head and neck cancer is on the rise globally, posing challenges for clinical management. It remains unclear which biomarker best reflects HPV-driven carcinogenesis, with P16-IHC performing the best as a single biomarker for prognosis stratification in OPSCC patients among the six potential HPV surrogate biomarkers tested in 153 HNSCC patients.
Article
Immunology
Junhong Shi, Meiyu Bao, Weifeng Wang, Xuan Wu, Yueying Li, Changdong Zhao, Weiwei Liu
Summary: PLOD3 is highly expressed in colorectal cancer and associated with advanced stage CRC and poor survival. High PLOD3 expression is correlated with low levels of immune cells such as B cells, CD4(+) T cells, M1 macrophages, and CD8(+) T cells. Patients with high PLOD3 expression may be resistant to immunotherapy.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Enfang Shan, Yi-nan Cao, Yang Zhang, Wen Chen, Xurui Ren, Shanjie Zhu, Xueru Xi, Shuai Mu, Mian Ma, Tongle Zhi, Xianwen Li
Summary: This study investigates the role of CACNG3 in the progression and survival outcome of gliomas and identifies it as a potential new molecular target for diagnosis and treatment. CACNG3 expression is negatively associated with glioma grades and is significantly reduced in certain molecular subtypes. Functional analysis suggests that CACNG3 may affect glioma development through interference with synaptic transmission. In addition, the commonly used glioma treatment, temozolomide, increases CACNG3 expression. Overall, CACNG3 plays a vital role in glioma occurrence and development and could serve as a biomarker for targeted therapy.
Article
Oncology
TengFei Deng, Chaoyong Wang, Cong Gao, Qiang Zhang, Jun Guo
Summary: This study explores the correlation between ITGAL expression, immune infiltration, clinical prognosis, and specific types of T cells in melanoma tissue. The findings suggest that ITGAL plays a crucial role in melanoma and has the potential to regulate tumor immune infiltrating cells. It may serve as a diagnostic biomarker and therapeutic target for advanced melanoma.
FRONTIERS IN ONCOLOGY
(2023)
Article
Health Care Sciences & Services
Pei-Hung Chang, Hung-Ming Wang, Yung-Chia Kuo, Li-Yu Lee, Chia-Jung Liao, Hsuan-Chih Kuo, Cheng-Lung Hsu, Chun-Ta Liao, Sanger Hung-Chi Lin, Pei-Wei Huang, Tyler Min-Hsien Wu, Jason Chia-Hsun Hsieh
Summary: The study investigated the prognostic value of p16-positive and p16-negative circulating tumor cells (CTCs) in head and neck squamous cell carcinoma (HNSCC) patients undergoing definitive chemoradiotherapy. The presence of p16-positive CTCs was associated with better responses to treatment and improved disease progression, while p16-negative CTCs were linked to disease progression. It suggested that CTC testing could help predict outcomes and guide treatment decisions in HNSCC patients.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Biology
Ning Wang, Yuanyuan Li, Xue Zhou, Xue Wang, Guoyue Yang
Summary: This study found that ARHGEF6 was significantly downregulated in lung adenocarcinoma tissues and had high diagnostic value. Low expression of ARHGEF6 was associated with more somatic mutations, worse tumor stage, and poor clinical prognosis. Experimental validation showed that low expression of ARHGEF6 inhibited cell proliferation and colony formation. Additionally, ARHGEF6 expression was positively correlated with immune cell infiltration and immune checkpoints. Overall, ARHGEF6 could be a potential prognostic and immunological biomarker in lung adenocarcinoma.
COMPUTERS IN BIOLOGY AND MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Feng Zhang, Shuijiao Guo, Wenhui Zhong, Kaijun Huang, Yubin Liu
Summary: The study demonstrated that metallothionein isoforms have aberrant expression and varying prognostic value in hepatocellular carcinoma. The repression of MT1H in HCC was found to be detrimental to patient outcomes.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Pathology
Toshio Ichiki, Yuichi Yamada, Takamichi Ito, Takeshi Nakahara, Yasuharu Nakashima, Masafumi Nakamura, Tomoharu Yoshizumi, Akira Shiose, Koichi Akashi, Yoshinao Oda
Summary: This study aimed to establish a method of predicting the prognosis of primary angiosarcoma. The study found that age, tumor type, metastasis, tumor size, and chemotherapy were factors related to prognosis. Through staining and immunohistochemical methods, tissue markers for angiosarcoma were identified to aid in predicting prognosis.
Article
Immunology
Zhenguo Cai, Yanjuan Duan, Wen Li, Zhuohang Liu, Zijun Gong, Sheng Hong, Xu He, Xinyang Xuanyuan, Youdong Chen, Xinling Bi, Wuqing Wang
Summary: This study found that high expression of FANCI in skin cutaneous melanoma (SKCM) is associated with poor prognosis. Knocking down FANCI can inhibit tumor cell proliferation and invasion, and affect the tumor immune environment. These findings provide a basis for predicting the immune status and treatment of SKCM.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Aaron M. Taylor, Jiayi M. Sun, Alexander Yu, Horatiu Voicu, Jianhe Shen, Donald A. Barkauskas, Timothy J. Triche, Julie M. Gastier-Foster, Tsz-Kwong Man, Ching C. Lau
Summary: This study revealed the heterogeneity of osteosarcoma in younger populations and identified several genes, such as IGF1R and FGFR2, that are associated with the pathogenesis of the disease. These genes were found to have a significant impact on the prognosis of patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Oncology
Malgorzata Gajdzis, Radoslaw Kaczmarek, Pawel Gajdzis
Summary: Uveal melanoma is the most common primary intraocular neoplasm in adults with a high rate of distant metastases and no effective treatment in the advanced stage. The search for new prognostic factors is crucial for understanding the disease's biology, monitoring patients effectively, and identifying potential therapeutic targets. Numerous studies have demonstrated the complexity of uveal melanoma biology and the potential for individualized treatment approaches based on protein expression patterns.
Letter
Oncology
Trine Engelbrecht Hybel, Maja Olholm Vase, Kristina Lystlund Lauridsen, Marie Beck Enemark, Michael Boe Moller, Court Pedersen, Gitte Pedersen, Stephen Hamilton-Dutoit, Niels Obel, Carsten Schade Larsen, Francesco d'Amore, Maja Ludvigsen
LEUKEMIA & LYMPHOMA
(2022)
Article
Oncology
Bent Honore, Maja Dam Andersen, Diani Wilken, Peter Kamper, Francesco d'Amore, Stephen Hamilton-Dutoit, Maja Ludvigsen
Summary: Patients with classic Hodgkin lymphoma (cHL) refractory to standard ABVD treatment show significant differences in protein expression, indicating potential biological distinctiveness between ABVD sensitive and refractory tumors. Further analysis of the tumor microenvironment and identification of prognostic biomarkers is crucial for improving outcomes.
Article
Pathology
Anita Tranberg Simonsen, Amalie Utke, Johanne Lade-Keller, Lasse Westphal Thomsen, Torben Steiniche, Magnus Stougaard
Summary: This study presents a small targeted expression panel with the potential to perform molecularly based histological classification and fusion gene identification in non-small-cell lung cancer (NSCLC) patients. The panel showed agreement with the original immunohistochemistry-based classification and successfully detected fusion transcripts in patients with fusion genes. Additionally, the molecular assessment of PD-L1 was consistent with the original assessment by immunohistochemistry.
EXPERIMENTAL AND MOLECULAR PATHOLOGY
(2022)
Article
Oncology
Lisa Lokso Dietz, Natasja Toft Furman, Trine Vilsboll Larsen, Tina Fuglsang Daugaard, Emil Aagaard Thomsen, Johanne Lade Keller, Lars Aagaard, Boe Sandahl Sorensen, Anders Lade Nielsen
Summary: Researchers have discovered eight different PD-L2 isoforms in non-small cell lung cancer cells, generated from the PDCD1LG2 gene through alternative splicing. The most prominent alternative splicing event is the extension of exon 6, resulting in the PD-L2 isoform V with a cytoplasmic domain extension. These observations of different cytoplasmic domains are important for further understanding of the PD-1 immune checkpoint pathway.
JOURNAL OF IMMUNOTHERAPY
(2022)
Article
Oncology
Cathrine F. F. Hjorth, Per Damkier, Tore B. B. Stage, Soren Feddersen, Stephen Hamilton-Dutoit, Bent Ejlertsen, Timothy L. L. Lash, Henrik Boggild, Henrik T. T. Sorensen, Deirdre Cronin-Fenton
Summary: This study found that single nucleotide polymorphisms may influence return-to-work and stable labor market attachment after breast cancer treatment, but overall, the SNPs examined did not have a significant impact on these outcomes in premenopausal women with breast cancer.
CANCER CHEMOTHERAPY AND PHARMACOLOGY
(2023)
Article
Pathology
Carina Mellemgaard, Ib Jarle Christensen, Giedrius Salkus, Pia Wirenfeldt Staun, Niels Korsgaard, Kim Hein Lindahl, Mathilde Skaarup Larsen, Siri Klausen, Johanne Lade-Keller
Summary: The aim of the study was to optimize the melanoma sentinel node (SN) pathology protocol by reducing the workload without compromising the metastasis detection rate. Through implementing a protocol that examines 3 levels 300μm apart combined with immunohistochemistry (IHC), it was found that the metastasis detection rate was similar to the protocol that examines 6 levels 150μm apart. Therefore, it is recommended that the future melanoma SN guidelines consider using the three-level method to reduce overall workload.
JOURNAL OF CLINICAL PATHOLOGY
(2023)
Article
Pathology
Ea Maria Tonning Tonnesen, Magnus Stougaard, Peter Meldgaard, Johanne Lade-Keller
Summary: This study investigates the association between oncogenic alterations and PD-L1 expression in lung adenocarcinomas, as well as the prognostic value of KRAS and/or TP53 mutations in patients treated with immunotherapy. The results suggest that KRAS mutations and higher expression of PD-L1 are associated. Additionally, concomitant TP53 and KRAS mutations may serve as a potential biomarker for improved survival with immunotherapy.
JOURNAL OF CLINICAL PATHOLOGY
(2022)
Correction
Gastroenterology & Hepatology
Peter Lykke Eriksen, Karen Louise Thomsen, Stephen Hamilton-Dutoit, Hendrik Vilstrup, Michael Sorensen
BMC GASTROENTEROLOGY
(2023)
Article
Hematology
Suvi-Katri Leivonen, Terhi Friman, Matias Autio, Samuli Vaittinen, Andreas Wind Jensen, Francesco d'Amore, Stephen Jacques Hamilton-Dutoit, Harald Holte, Klaus Beiske, Panu E. Kovanen, Riikka Raty, Sirpa Leppa
Summary: This study provides a comprehensive phenotypic characterization of post-transplant lymphoproliferative disorders (PTLD), highlighting the importance of immune cell composition in determining the clinical behavior and prognosis of PTLD.
Article
Multidisciplinary Sciences
Tapashi Dalvi, Mette Norgaard, Jon P. Fryzek, Naimisha Movva, Lars Pedersen, Hanh Pham Hansen, Jill Walker, Anita Midha, Norah Shire, Anne-Marie Boothman, James Rigas, Anders Mellemgaard, Torben R. Rasmussen, Stephen Hamilton-Dutoit, Deirdre Cronin-Fenton
Summary: This study found no prognostic impact of PD-L1 expression, EGFR mutations, and KRAS mutations in NSCLC patients receiving adjuvant chemotherapy.
Article
Oncology
Trine V. Larsen, Nina Dybdal, Tina F. Daugaard, Johanne Lade-Keller, Lin Lin, Boe S. Sorensen, Anders L. Nielsen
Summary: This study investigates the role of PD-L1 DNA methylation in regulating and predicting PD-L1 expression in non-small-cell lung cancer (NSCLC) patients undergoing immunotherapy. The analysis of tumor biopsies and cell lines reveals that PD-L1 DNA methylation status influences its expression. However, the correlation between methylation and expression is weak in NSCLC tumor samples. This study emphasizes the importance of further research to improve the effectiveness of PD-1/PD-L1 immunotherapy in NSCLC.
Article
Biochemistry & Molecular Biology
Emmeli Mikkelsen, Berthold Huppertz, Ripudaman Singh, Katarina Ravn, Lotte Hatt, Mogens Kruhoffer, Rheanna Urrabaz-Garza, Niels Uldbjerg, Ramkumar Menon, Torben Steiniche
Summary: Unique markers in fetal membrane cells may be key in the search for biomarkers for preterm prelabor rupture of the fetal membranes (pPROM) in maternal blood. Through transcriptomic analysis and immunohistochemistry, markers exclusively expressed in fetal membranes were identified and their localization confirmed. These findings contribute to the understanding of the biological significance of these markers in the maternal circulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Lene Ugilt Pagter Ludvigsen, Annemette Sloth Andersen, Stephen Hamilton-Dutoit, Soren Jensen-Fangel, Pernille Bottger, Kurt Jensen Handberg, Per Ivarsen, Francesco d'Amore, Bo Martin Bibby, Birgitte Klug Albertsen, Bente Jespersen, Marianne Kragh Thomsen
Summary: WHO EBV-qPCR in plasma and whole blood has a low positive predictive value but a high negative predictive value for proven EBV disease.
JOURNAL OF CLINICAL VIROLOGY
(2023)
Article
Genetics & Heredity
Michelle Mohlenberg, Peter Lykke Eriksen, Tea Lund Laursen, Mette Bak Nielsen, Stephen Jacques Hamilton Dutoit, Henning Gronbaek, Rune Hartmann, Karen Louise Thomsen
Summary: In this study, the relationship between innate immune mechanisms and fibrosis development in non-alcoholic fatty liver disease (NAFLD) patients was investigated. It was found that the non-functional IFNL4 allele was overrepresented in patients with significant fibrosis. In addition, the potential protective role of interferon (IFN) in relation to liver fibrosis development in an animal model contradicted the previous hypothesis.
GENES AND IMMUNITY
(2022)
Article
Otorhinolaryngology
Alexander Wieck Fjaeldstad, Gerda Elisabeth Villadsen, Gitte Dam, Stephen Jacques Hamilton-Dutoit, Thomas Winther Frederiksen
Summary: A 55-year-old man presented with progressive conductive hearing loss and an external auditory canal tumor. After surgical removal, the tumor was unexpectedly identified as a well-differentiated neuroendocrine tumor through histological examination and PET/CT scans. This case is unique in that it had exostosis-like bone growth underneath the tumor, a feature not previously reported.
OTOLARYNGOLOGY CASE REPORTS
(2022)