Article
Multidisciplinary Sciences
Harim Oh, Chul Hwan Kim, Yoo Jin Lee
Summary: The study evaluated the diagnostic value of immunohistochemical staining for bullous pemphigoid (BP) detection, showing that IHC staining has high sensitivity and specificity, being helpful for the diagnosis of DIF-confirmed BP cases and clinically suspected BP cases.
SCIENTIFIC REPORTS
(2022)
Article
Pathology
Ivan Chebib, Martin S. Taylor, Valentina Nardi, Miguel N. Rivera, Jochen K. Lennerz, Gregory M. Cote, Edwin Choy, Santiago A. Lozano Calderon, Kevin A. Raskin, Joseph H. Schwab, John T. Mullen, Yen-Lin E. Chen, Yin P. Hung, Gunnlaugur P. Nielsen, Vikram Deshpande
Summary: The study evaluated the role of gene fusion assay in sarcoma diagnostics, showing a high concordance rate of 83% with morphology/immunohistochemistry. The fusion assay performed well in identifying fusions of uncertain significance, but integration with morphology, immunohistochemical profile, and molecular methods is essential for accurate diagnosis and optimal clinical management of sarcomas.
AMERICAN JOURNAL OF SURGICAL PATHOLOGY
(2021)
Article
Chemistry, Analytical
Hao Chen, Yuefei Zhang, Haichao Zhou, Weiran Chen, Jiayi Peng, Yang Feng, Linyuan Fan, Jun Li, Jin Zi, Yan Ren, Qidan Li, Siqi Liu
Summary: Spatial proteomics plays an important role in the era of single-cell biology. In this study, a new routine workflow was developed for proteomic analysis of micro-FFPE tissues, allowing the construction of a spatial proteomic atlas of mouse brain, which exhibited typical characteristics of spatial-dependent protein abundance and functional enrichment.
ANALYTICAL CHEMISTRY
(2023)
Article
Oncology
Nicolas Macagno, Daniel Pissaloux, Arnaud de la Fouchardiere, Marie Karanian, Sylvie Lantuejoul, Francoise Galateau Salle, Alexandra Meurgey, Catherine Chassagne-Clement, Isabelle Treilleux, Caroline Renard, Juliette Roussel, Julie Gervasoni, Vincent Cockenpot, Carole Crozes, Aline Baltres, Aurelie Houlier, Sandrine Paindavoine, Laurent Alberti, Adeline Duc, Francois Le Loarer, Armelle Dufresne, Mehdi Brahmi, Nadege Corradini, Jean-Yves Blay, Franck Tirode
Summary: Many neoplasms remain unclassified after histopathological examination and require further molecular analysis. Mesenchymal neoplasms are particularly challenging due to their rarity and the large number of subtypes, with many lacking reliable diagnostic markers. RNA transcriptomic profiles have proved to be a reliable basis for the classification of previously unclassified tumors, especially for mesenchymal neoplasms.
GENES CHROMOSOMES & CANCER
(2022)
Article
Oncology
George S. Tamvakopoulos, Barry Rose, Asif Saifuddin, John A. Skinner, Robin Pollock
Summary: Re-biopsy after initial non-diagnostic core needle biopsy showed high sensitivity and specificity, particularly in the presence of malignancy. In the absence of tissue diagnosis, multidisciplinary team assessment also proved to be highly accurate and a safe strategy in managing this complex group of patients.
Article
Automation & Control Systems
Lorena Guachi-Guachi, Jacopo Ruspi, Paola Scarlino, Aliria Poliziani, Sabrina Ciancia, Dario Lunni, Gabriele Baldi, Andrea Cavazzana, Alessandra Zucca, Marco Bellini, Gian Andrea Pedrazzini, Gastone Ciuti, Marco Controzzi, Lorenzo Vannozzi, Leonardo Ricotti
Summary: This research presents an automated method for tissue block processing by analyzing surface images of each cutting routine. The method uses Convolutional Neural Networks for image segmentation and classification tasks, demonstrating its potential in histopathology. The results show that the proposed method accurately identifies the trimming-end cutting routine and performs similarly to skilled technicians.
ENGINEERING APPLICATIONS OF ARTIFICIAL INTELLIGENCE
(2023)
Article
Biotechnology & Applied Microbiology
Liang Zong, Yabing Zhu, Yuan Jiang, Ying Xia, Qun Liu, Sanjie Jiang
Summary: RNA-Seq analysis of FFPE samples is a highly effective approach but degradation of RNAs limits its application. A new RNA-Seq method has been developed to overcome this limitation. This study compares and evaluates three commercially available exome capture kits, providing valuable guidance for obtaining high-quality data from FFPE samples.
Article
Cell Biology
Cooper D. Rutland, Jodi Gedallovich, Aihui Wang, Sabrina Zdravkovic, Sushama Varma, Jason L. Hornick, Gregory W. Charville
Summary: This study aimed to explore the diagnostic utility of FOXO1 immunohistochemistry for rhabdomyosarcoma classification. The findings suggest that FOXO1 immunohistochemistry is a highly sensitive and relatively specific surrogate marker of the PAX3/7::FOXO1 fusion oncoprotein in rhabdomyosarcoma.
Article
Microbiology
Alexis Trecourt, Meja Rabodonirina, Claire Mauduit, Alexandra Traverse-Glehen, Mojgan Devouassoux-Shisheboran, David Meyronet, Frederique Dijoud, Christophe Ginevra, Emmanuelle Chapey-Picq, Emilie Josse, Patricia Martins-Simoes, Abderrazzak Bentaher, Damien Dupont, Charline Miossec, Florence Persat, Martine Wallon, Tristan Ferry, Felix Pham, Bruno Simon, Jean Menotti
Summary: A targeted next-generation sequencing (NGS) method was developed to achieve a fungal integrated histomolecular diagnosis using formalin-fixed tissue samples (FTs), improving the accuracy of fungal detection and identification.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Integrative & Complementary Medicine
Jing Wei, Tianyu Chen, Xiaolin Chen, Ganzhu Feng
Summary: In this study, the diagnostic value of three different tests for tuberculous pleurisy (TP) was compared, and a new test called paraffin-embedded sample test (PEST) was found to have a high sensitivity and overall diagnostic value for TP diagnosis.
ALTERNATIVE THERAPIES IN HEALTH AND MEDICINE
(2023)
Review
Clinical Neurology
Michael D. Shost, Tarun K. Jella, Andrew A. Ronald, Eric Z. Herring, Martha Sajatovic, Gabriel A. Smith
Summary: Our analysis found that routine imaging after spinal fusion surgery had no direct benefit on clinical management. Further research is necessary to identify optimal imaging criteria following spinal fusion surgery.
WORLD NEUROSURGERY
(2022)
Review
Medicine, General & Internal
Stefan K. Zoellner, James E. Amatruda, Sebastian Bauer, Stephane Collaud, Enrique de Alava, Steven G. DuBois, Jendrik Hardes, Wolfgang Hartmann, Heinrich Kovar, Markus Metzler, David S. Shulman, Arne Streitbuerger, Beate Timmermann, Jeffrey A. Toretsky, Yasmin Uhlenbruch, Volker Vieth, Thomas G. P. Gruenewald, Uta Dirksen
Summary: Ewing sarcoma is a highly aggressive bone and soft-tissue cancer with a genetically simple yet specific therapeutic target. Current standard treatment involving systemic therapy and local treatment provides a realistic chance of cure for the majority of patients.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Oncology
Joanna Ka Man Ng, Chit Chow, Ronald Cheong Kin Chan, Ka Pang Chan, Joshua Jing Xi Li, Molly Siu Ching Li, Ka-Fai To
Summary: In this study, a large cohort of EGFR tests was reviewed to evaluate the adequacy, detection rate, and discrepancy rate of cytology material. The results indicate that cytology material has lower adequacy rates, while pleural fluid is superior to pleural biopsies for molecular testing.
Article
Oncology
Naoko Hosono, SungGi Chi, Takahiro Yamauchi, Kentaro Fukushima, Hirohiko Shibayama, Seiichiro Katagiri, Akihiko Gotoh, Motoki Eguchi, Takanobu Morishita, Reiki Ogasawara, Takeshi Kondo, Masamitsu Yanada, Kazuhito Yamamoto, Tsutomu Kobayashi, Junya Kuroda, Kensuke Usuki, Yoshikazu Utsu, Makoto Yoshimitsu, Kenji Ishitsuka, Takaaki Ono, Naoto Takahashi, Satoshi Iyama, Kensuke Kojima, Yukinori Nakamura, Suguru Fukuhara, Koji Izutsu, Hikaru Abutani, Nobuhiko Yamauchi, Junichiro Yuda, Yosuke Minami
Summary: This multicenter study used paraffin-embedded bone marrow clot specimens to detect actionable mutations in newly diagnosed unfit and relapsed/refractory AML patients. Targeted sequencing detected genetic alterations in 97.3% of patients and fusion transcripts in 23.2% of patients. Useful genetic mutations for treatment selection were found in 38% of patients.
Article
Environmental Sciences
Ali Ibrahim, Ahmed El-Sebaii, Saad Aboul-Enein, Mohammed Hegazy, Assem Fleafl, Abd EL-Monem Khallaf
Summary: This study investigates the performance of a wick-type solar still using phase change material and titanium dioxide nanoparticles as a storage material. The addition of nanoparticles increases the thermal conductivity of the material, enhancing productivity and efficiency.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)