Article
Biochemistry & Molecular Biology
Leilei Zhou, Yingxuan Ma, Silin Zhong, Jiankang Cao, Yunbo Luo, Guiqin Qu
Summary: Plant growth and immunity are interconnected, and this study investigates the effect of oligogalacturonic acids (OGs) on plant growth. The results show that OGs inhibit the growth of intact etiolated seedlings in tomato plants. The inhibition is partially suppressed by ethylene biosynthesis inhibitors and gene silencing of the rate-limiting enzyme for ethylene biosynthesis, SlACS2. The study also reveals the involvement of SlACS2 phosphorylation and the mitogen-activated protein kinase SlMPK3 in OG-induced growth inhibition.
Article
Plant Sciences
Kangqi Sang, Junjie Li, Xiangjie Qian, Jingquan Yu, Yanhong Zhou, Xiaojian Xia
Summary: Brassinosteroids play critical roles in fruit ripening, and ethylene signaling regulates BR synthesis and signaling through AP2a mediation.
Article
Plant Sciences
Adriana E. Cisneros, Purificacion Lison, Laura Campos, Joan Manel Lopez-Tubau, Teresa Altabella, Albert Ferrer, Jose-Antonio Daros, Alberto Carbonell
Summary: This study aimed to engineer highly resistant tomato plants to Potato spindle tuber viroid (PSTVd) and developed tomato lines expressing an artificial microRNA (amiRNA) against PSTVd (amiR-PSTVd). However, the results showed that amiR-PSTVd-expressing lines were hypersusceptible to the viroid instead of being resistant. Further analysis revealed that amiR-PSTVd accidentally silenced the tomato STEROL GLYCOSYLTRANSFERASE 1 (SlSGT1) gene, which led to late developmental and reproductive defects as well as hypersusceptibility to viroid infection.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Mineyuki Yokoyama, Takatoshi Yamashita, Rumi Kaida, Shigemi Seo, Kazuhiro Tanaka, Sadao Abe, Masataka Nakano, Yoshiharu Fujii, Kazuyuki Kuchitsu
Summary: Water containing ultrafine/nano bubbles (UFBs) can promote plant growth in soil damaged by previous cultivation, but does not affect plant growth in rock wool or healthy soil. Additionally, UFB water treatment can partly suppress the growth of the pathogen of bacteria wilt disease.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
Article
Developmental Biology
Brendan J. Houston, Alexandra M. Lopes, Maris Laan, Liina Nagirnaja, Anne E. O'Connor, D. Jo Merriner, Joseph Nguyen, Margus Punab, Antoni Riera-Escamilla, Csilla Krausz, Kenneth Ivan Aston, Donald F. Conrad, Moira K. O'Bryan
Summary: This study found that one of the genes associated with male infertility, DCAF12L1, does not have a significant impact on fertility in mice. This disparity may be due to differences in DCAF12 family members between mice and humans, or the presence of environmental factors in humans.
DEVELOPMENTAL BIOLOGY
(2022)
Article
Plant Sciences
Angel Chavez, Nidia Castillo, Joan Manel Lopez-Tubau, Kostadin E. Atanasov, Emma Fernandez-Crespo, Gemma Camanes, Teresa Altabella, Albert Ferrer
Summary: Free and glycosylated sterols in the plant cell plasma membrane regulate its structure and function, with glycosylated sterols being more important in plants like tomato. Silencing STEROL GLYCOSYLTRANSFERASE 1 in tomato affects plant growth and fruit development, with reduced levels of glycosylated sterols triggering a downregulatory response that affects critical processes for fruit development.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Biochemical Research Methods
Eugenio Llorens, Loredana Scalschi, Ana Gonzalez-Hernandez, Gemma Camanes, Pilar Garcia-Agustin, Begonya Vicedo
Summary: The study demonstrates that the activation of induced resistance in plants leads to an increase in defensive proteins, altering the composition of the apoplastic fluid to enhance defense against pathogen invasion. Proteomic analysis showed changes in protein composition in the apoplast after treatment and infection, with identified proteins playing a role in plant protection. Additionally, one of the over-accumulated proteins showed antimicrobial activity against Pseudomonas syringae pv. tomato in vitro.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Plant Sciences
Xiaowei Yin, Mingjia Tang, Xiaojian Xia, Jingquan Yu
Summary: In this study, the researchers investigated the role of brassinosteroids (BRs), a type of plant hormone, in regulating the Calvin cycle genes in tomatoes. They found that BRs can enhance the activity of the Calvin cycle and improve photosynthetic ability. This regulation is mediated by the signal effector BRASSINAZOLE RESISTANT 1 (BZR1), which directly binds to the promoters of key Calvin cycle genes. These findings suggest that BRs regulate the Calvin cycle in a BZR1-dependent manner in tomatoes, highlighting the potential of targeting BRs to increase crop yields.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Kapil Sharma, Soni Gupta, Supriya Sarma, Meenakshi Rai, Yellamaraju Sreelakshmi, Rameshwar Sharma
Summary: This study reveals the role of ethylene in the development of tomato plants, not only in ripening but also in vegetative and reproductive processes. Mutants acs2-1 and acs2-2 exhibit contrasting ethylene emission levels and responses, impacting physiological and metabolic profiles of tomatoes.
Article
Biochemistry & Molecular Biology
Tomoki Shibuya, Manabu Nishiyama, Kazuhisa Kato, Yoshinori Kanayama
Summary: SlFKF1, a homolog of FKF1 in tomato, a day-neutral plant with fleshy fruit, may have similar functions to Arabidopsis thaliana FKF1. It is involved in the regulation of flowering and fruit ripening, and its expression is influenced by blue light. This study provides insights into the diverse physiological roles of FKF1 in plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Man Bo Lee, Reza Shekasteband, Samuel F. Hutton, Tong Geon Lee
Summary: Reducing plant height can improve crop yield potential, resilience to stresses, and adoption of management practices. Breeding tomato plants with reduced height is challenging due to the potential decrease in fruit size. This study identified the BR locus that controls plant height in tomatoes and discovered three FPF1 genes within this genomic region. A complete deletion of the FPF1 gene (Solyc01g066980) was found in the br allele but not in BR. Knocking out Solyc01g066980 in large-fruited tomatoes reduced height and yield but retained the ability to produce large fruits. The study also showed that artificial selection contributes to commercially acceptable yield potential in br tomatoes. Network analysis of gene expression patterns revealed the involvement of FPF1 genes in suppressing gibberellic acid biosynthesis in roots. Mutations in br homologs also resulted in reduced height. These findings provide insights into the genetic and physiological mechanisms underlying tomato architecture changes caused by the br allele.
Article
Plant Sciences
Chang-Jiao Ke, Xian-Ju Lin, Bao-Yu Zhang, Li-Yu Chen
Summary: The study demonstrates the conservative role of TOD1 in angiosperms, involving in pollen tube growth by regulating sphingolipid biosynthesis. The findings reveal the expression of OsTOD1 in rice and its effect on pollen tube growth.
Article
Plant Sciences
X. Liu, Y. Gao, K. Li, Y. Yin, J. Liu, Y. Zhu
Summary: The NPF gene family in tomato exhibits diversity and shows different transcriptional responses under plant nitrogen status. Whole-genome triplication and tandem duplication are major contributions to the expansion of SlNPF genes. The identification of 19 orthologous genes suggests the presence of at least 19 NPF genes in a common ancestor before Arabidopsis and tomato diverged.
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
(2022)
Review
Medical Laboratory Technology
Ying Chen, Shengxiong Huang, Liuyan Zhou, Xin Wang, Huan Yang, Wenqing Li
Summary: The ongoing COVID-19 pandemic has posed a new challenge for public health, necessitating the development of fast and efficient detection methods. Common diagnostic methods include RT-qPCR, CT scans, and new-generation sequencing technologies, with researchers worldwide working on developing other rapid detection methods.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2022)
Article
Biochemistry & Molecular Biology
Linchao Wang, Bo Liu, Yuruo Yang, Qiguo Zhuang, Shijia Chen, Yongsheng Liu, Shengxiong Huang
Summary: This study conducted comprehensive comparative analyses of 29 independent chloroplast genomes from Actinidia species, revealing the causes of genome size expansion and identifying variation hot spots in specific gene loci. These findings contribute to a better understanding of chloroplast genome evolution and phylogenetic relationships among Actinidia species.
MOLECULAR GENETICS AND GENOMICS
(2022)
Article
Plant Sciences
Xiujing Hong, Shengxiong Huang, Yongsheng Liu
Summary: Nigrospora sphaerica is a worldwide plant pathogen causing diseases on various plant hosts. This study reports the genome resource of N. sphaerica strain ZJJ-C1, which causes fruit dried-shrink disease in Akebia trifoliata in China. The genome sequence of ZJJ-C1 will be beneficial for studying the evolution, host adaptation, and pathogenicity of N. sphaerica, contributing to a better understanding of host-pathogen interaction mechanisms.
Article
Genetics & Heredity
Yuxiang Li, Haiyang Xu, Javed M. Chitaman, Jian Feng
Summary: DNA methylation plays important roles in cellular processes, but traditional methods have limitations in studying specific cell types. This study applied two new methods to analyze the methylome of a specific type of neuron in the mouse brain, and found high-quality data. Additionally, the study identified cell type-specific regulatory regions and compared methylomes of different cell types.
Article
Biotechnology & Applied Microbiology
Zhaoyuan Lian, Chi Dinh Nguyen, Li Liu, Guiluan Wang, Jianjun Chen, Songhu Wang, Ganjun Yi, Sandra Wilson, Peggy Ozias-Akins, Haijun Gong, Heqiang Huo
Summary: This study demonstrates that manipulation of the PLT5 gene can effectively improve in planta and in vitro transformation efficiency, and such a transformation system can be used to facilitate the application of genome editing or other plant biotechnology in modern agriculture.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Heng Deng, Yao Chen, Ziyu Liu, Zhaoqiao Liu, Peng Shu, Ruochen Wang, Yanwei Hao, Dan Su, Julien Pirrello, Yongsheng Liu, Zhengguo Li, Don Grierson, James J. Giovannoni, Mondher Bouzayen, Mingchun Liu
Summary: This study reveals that SlERF.F12, an ethylene response factor, negatively regulates the initiation of tomato fruit ripening by recruiting a co-repressor and histone deacetylases. SlERF.F12 interacts with the co-repressor and recruits histone deacetylases to form a complex, which decreases the acetylation level at the promoter regions of ripening genes, thus repressing their transcription.
Article
Agriculture, Multidisciplinary
Guo Xiu-hong, He Yan, Zhang Yu, Wang Yi, Huang Sheng-xiong, Liu Yong-sheng, Li Wei
Summary: This study identified and characterized two cytosolic APX genes (AcAPX1 and AcAPX2) in kiwifruit. The expression pattern and response to NaCl treatment were determined for both AcAPX1 and AcAPX2. The subcellular localization assays showed that both AcAPX1 and AcAPX2 are targeted to the cytosol. Over-expression of AcAPX1 or AcAPX2 increased total ascorbic acid levels and salinity tolerance in Arabidopsis thaliana.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2022)
Article
Plant Sciences
Lihuan Wang, Xiaofeng Tang, Shiqiang Zhang, Xiang Xie, Mengfei Li, Yongsheng Liu, Songhu Wang
Summary: The CsGLK genes in tea plants are involved in light-regulated catechin accumulation. They promote catechin biosynthesis by regulating the expression of the transcription factor CsMYB5b. They also play a role in promoting chloroplast development and carotenoid accumulation in tomato plants.
HORTICULTURE RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Jun Yang, Chengcheng Ling, Yunyan Liu, Huamin Zhang, Quaid Hussain, Shiheng Lyu, Songhu Wang, Yongsheng Liu
Summary: The study comprehensively analyzed the RFS and GolS gene families in kiwifruit and revealed their roles in abiotic and biotic stress responses. Transcriptomic analysis and functional experiments confirmed the importance of some crucial genes, and identified one key gene (AcRFS4) that showed significantly increased transcription levels under salt stress and enhanced salt tolerance in kiwifruit.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Dan Su, Kaidong Liu, Zhuoshu Yu, Ying Li, Yaoxin Zhang, Yunqi Zhu, Yi Wu, Hongyu He, Xiaodan Zeng, Honglin Chen, Don Grierson, Heng Deng, Mingchun Liu
Summary: In this study, 17 GA-responsive genes (GASA) were identified in tomato. Among them, SlGASA1 gene was found to have a negative role in regulating fruit ripening, as its expression levels were low during fruit ripening. Overexpressing SlGASA1 delayed fruit ripening, and it was found to interact with the key ripening regulator FRUITFULL1 and repress its activation of ethylene biosynthesis genes ACS2 and ACO1.
HORTICULTURE RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Zhijian Liu, Yongzhi Hu, Anping Du, Lan Yu, Xingyue Fu, Cuili Wu, Longxiang Lu, Yangxuan Liu, Songhu Wang, Weizao Huang, Shengbin Tu, Xinrong Ma, Hui Li
Summary: Salt-alkali stress is a threat to rice resilience and grain yield. This study identified a novel salt-alkali-tolerant rice (SATR) and revealed the potential role of cell wall matrix polysaccharides in mediating SATR's strong tolerance. The gene OsCSLD4 in the hemicellulose biosynthesis pathway was found to play a crucial role in SATR's SSAS tolerance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Xiaoyu Tu, Sibo Ren, Wei Shen, Jianjian Li, Yuxiang Li, Chuanshun Li, Yangmeihui Li, Zhanxiang Zong, Weibo Xie, Donald Grierson, Zhangjun Fei, Jim Giovannoni, Pinghua Li, Silin Zhong
Summary: Non-coding cis-regulatory variants play a crucial role in the evolution of transcription regulation and phenotype diversity in animal genomes. However, the dynamics of cistrome in plants are not well understood. This study compares the binding of GLK transcription factors in different plant species and reveals that while the function of GLKs is conserved, their binding sites are mainly species-specific. The study also highlights the importance of quantitative measurement of TF occupancy.
NATURE COMMUNICATIONS
(2022)
Article
Plant Sciences
Junyang Yue, Qinyao Chen, Yingzhen Wang, Lei Zhang, Chen Ye, Xu Wang, Shuo Cao, Yunzhi Lin, Wei Huang, He Xian, Hongyan Qin, Yanli Wang, Sijia Zhang, Ying Wu, Songhu Wang, Yi Yue, Yongsheng Liu
Summary: This study reports a highly continuous and completely gap-free reference genome of Actinidia chinensis cv. 'Hongyang'. The integrity and contiguity of this new genome version are markedly improved compared to previous versions. The availability of this gap-free genome lays a solid foundation for genome structure and functional genomics studies, as well as for kiwifruit breeding and improvement.
HORTICULTURE RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Tonghao Miao, Huaxu Bao, Hui Ling, Pengwei Li, Yiling Zhang, Yan He, Xufan Hu, Chengcheng Ling, Yunyan Liu, Wei Tang, Yajing Liu, Songhu Wang
Summary: In this study, researchers found that the expression of the AlNAP1 gene, which plays a role in trichome development in kiwifruit, is suppressed in the species with short and distorted trichomes. They also discovered the presence of short transcripts lacking multiple exons in addition to the full-length transcript of AlNAP1. The alternative splicing of AlNAP1 further reduces the level of functional transcripts. These findings suggest that AlNAP1 mediates trichome development in kiwifruit and could be a candidate target for genetic modification of trichome length.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Bo Liu, Wenpeng Song, Linchao Wang, Yantao Wu, Xiaoting Xu, Xiangli Niu, Shengxiong Huang, Yongsheng Liu, Wei Tang
Summary: Little is known about the pathogenic genes of Pseudomonas syringae pv. actinidiae (Psa), the bacteria that cause bacterial canker of kiwifruit. This study successfully utilized the dCas9-BE3 and dCas12a-BE3 systems to perform gene editing in Psa, including single nucleotide substitutions and conversion of codons. Additionally, a highly efficient gene knockout system was developed, which can knockout multiple genes simultaneously in the Psa genome.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)