期刊
ACTA BIOMATERIALIA
卷 23, 期 -, 页码 38-51出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2015.05.016
关键词
Nanoparticle; Polyethylene; Bioactivity; Osteolysis; Joint replacement
资金
- DePuy International
- China Scholarship Council
- NIHR - United Kingdom (National Institute for Health Research) as a part of LMBRU (Leeds Musculoskeletal Biomedical Research Unit)
- Leeds Centre of Excellence in Medical Engineering - Wellcome Trust
- EPSRC [WT088908/z/09/z]
- Engineering and Physical Sciences Research Council [EP/G032483/1, EP/I019103/1, EP/K029592/1, EP/J017620/1, EP/N00941X/1] Funding Source: researchfish
- National Institute for Health Research [NF-SI-0611-10096] Funding Source: researchfish
- EPSRC [EP/I019103/1, EP/N00941X/1, EP/G032483/1, EP/J017620/1, EP/K029592/1] Funding Source: UKRI
Recently, nanometre-sized UHMWPE particles generated from hip and knee replacements have been identified in vitro and in vivo. UHMWPE particles in the 0.1-1.0 mu m size range have been shown to be more biologically active than larger particles, provoking an inflammatory response implicated in late aseptic loosening of total joint replacements. The biological activity of nanometre-sized particles has not previously been studied. The biological response to clinically-relevant UHMWPE wear particles including nanometre-sized and micrometre-sized, along with polystyrene particles (FluoSpheres 20 nm, 60 nm, 200 nm and 1.0 mu m), and nanometre-sized model polyethylene particles (Ceridust 3615 (R)), was determined in terms of osteolytic cytokine release from primary human peripheral blood mononuclear cells (PBMNCs). Nanometre-sized UHMWPE wear particles, nanometre-sized Ceridust 3615 (R) and 20 nm FluoSpheres had no significant effect on TNF-alpha, IL-1 beta, IL-6 and IL-8 release from PBMNCs at a concentration of 100 mu m(3) particles per cell after 12 and 24 h. The micrometre-size UHMWPE wear particles (0.1-1.0 mu m) and 60 nm, 200 nm and 1.0 mu m FluoSpheres caused significantly elevated osteolytic cytokine release from PBMNCs. These results indicated that particles below circa 50 nm fail to activate PBMNCs and that particle size, composition and morphology played a crucial role in cytokine release by particle stimulated macrophages. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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