Article
Multidisciplinary Sciences
Anouk Mentink, Khrystany T. Isebia, Jaco Kraan, Leon W. M. M. Terstappen, Michiel Stevens
Summary: When evaluating EpCAM-based enrichment technologies for CTCs, it is important to use cell lines that closely resemble real CTCs and measure the EpCAM expression of both CTCs and cell lines at different institutions and times. In this study, CTCs were enriched by depleting leukocytes from diagnostic leukapheresis products and EpCAM expression was measured using quantitative flow cytometry. Results showed varying but relatively low EpCAM expression in CTCs from castration-sensitive prostate cancer patients. There were also significant variations in antigen expression among identical cell lines cultured at different institutions, affecting capture efficiency.
SCIENTIFIC REPORTS
(2023)
Article
Engineering, Biomedical
Adria Sales, Valia Khodr, Paul Machillot, Line Chaar, Laure Fourel, Amaris Guevara-Garcia, Elisa Migliorini, Corinne Albiges-Rizo, Catherine Picart
Summary: By studying the presentation of matrix-bound BMPs on films of controlled stiffness, it was found that different BMP members have specific effects on cell adhesion and differentiation of skeletal progenitors. This response involves certain BMP receptors and beta chain integrins and is dependent on the stiffness of the substrate.
Review
Biochemistry & Molecular Biology
Duygu Sari-Ak, Alvaro Torres-Gomez, Yavuz-Furkan Yazicioglu, Anthos Christofides, Nikolaos Patsoukis, Esther M. Lafuente, Vassiliki A. Boussiotis
Summary: This review discusses the structural and molecular properties of RIAM and the functional role of the Rap1/RIAM module in hematopoietic cells.
BIOMEDICAL JOURNAL
(2022)
Article
Immunology
Jae-Young Park, Hyo-Min Park, Seonhwa Kim, Kyeong-Bae Jeon, Chae-Min Lim, Jin Tae Hong, Do-Young Yoon
Summary: IL-32 theta A94V, a mutant isoform, can inhibit monocyte-endothelial adhesion by binding to integrins aVb3 and aVb6, thus reducing the early step of atherosclerosis.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Mihai Adrian Bodescu, Jonas Aretz, Marco Grison, Matthias Rief, Reinhard Faessler
Summary: In this study, the mechanical stability of the talin-integrin bond was investigated using single-molecule force spectroscopy. The addition of kindlin-2 resulted in a force-independent, ideal talin-integrin bond, which relied on the steric proximity of and the intervening amino acid sequences between the talin and kindlin binding sites in the P-integrin tail. These findings reveal how kindlin cooperates with talin to enable the transmission of high forces required for cell adhesion.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Olegs Borodins, Felix Broghammer, Michael Seifert, Nils Cordes
Summary: Cancer is a highly heterogeneous disease with (epi)genetic characteristics that determine resistance to therapy. Preclinical research has identified the adhesome as a critical resistance mechanism in multiple cancers. This study connects preclinical data with transcriptomic and patient survival data, revealing a set of overexpressed genes in different cancers that negatively affect patient survival, particularly in radiotherapy-treated cohorts. These genes, including key integrins and their interconnectors, may serve as therapeutic targets in cancer treatment.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Xue Qiao, Conghui Liu, Weilin Wang, Chuanyan Yang, Meijia Li, Qilin Yi, Ning Kong, Limei Qiu, Xiyang Liu, Lingling Wang, Linsheng Song
Summary: Neural cell adhesion molecules (NCAMs) are large cell-surface glycoproteins that play important roles in cell-cell and cell-extracellular matrix interactions in the nervous system. A recent study identified a homologue of NCAM (CgNCAM) in the Pacific oyster Crassostrea gigas. CgNCAM was found to be widely distributed in oyster tissues, particularly in the mantle, labial palp, and haemolymph. It showed increased expression in oyster haemocytes after stimulation with Vibrio splendidus and Staphylococcus aureus.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Oncology
Claudia Owczarek, Elena Ortiz-Zapater, Jana Kim, Efthymia Papaevangelou, George Santis, Maddy Parsons
Summary: The coxsackie and adenovirus receptor (CAR) is highly expressed in lung cancer and may promote tumor growth and regulate epithelial mesenchymal transition (EMT). Our study demonstrates that CAR contributes to lung cancer metastasis through promoting cell-matrix adhesion, providing new insights into the cooperation between cell-cell and cell-matrix proteins in tumorigenesis.
FRONTIERS IN ONCOLOGY
(2022)
Article
Engineering, Biomedical
Neetu Kushwah, Kevin Woeppel, Vaishnavi Dhawan, Delin Shi, Xinyan Tracy Cui
Summary: The study demonstrates that L1 coating can reduce superoxide production from cultured microglia through direct reduction reaction and signaling pathways, and increase anti-inflammatory cytokine release and phagocytosis in response to PMA or LPS stimulation. Additionally, surface roughening with nanoparticles prior to L1 immobilization further enhances the beneficial effects of L1 in reducing microglia activation and oxidative stress.
ACTA BIOMATERIALIA
(2022)
Article
Immunology
Ofer Regev, Marina Kizner, Francesco Roncato, Maya Dadiani, Massimo Saini, Francesc Castro-Giner, Olga Yajuk, Stav Kozlovski, Nehora Levi, Yoseph Addadi, Ofra Golani, Shifra Ben-Dor, Zvi Granot, Nicola Aceto, Ronen Alon
Summary: ICAM-1 expressed by breast cancer cells is involved in innate immunity and can inhibit intravascular breast cancer metastasis to lungs.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Victoria Levario-Diaz, Rebecca Elizabeth Alvarado, Cristina Marcela Rodriguez-Quinteros, Andreas Fink, Joel Christian, Wenqian Feng, Elisabetta Ada Cavalcanti-Adam
Summary: This study describes a novel method to fabricate 1D micro-nanopatterned stripes, and reveals the migratory behavior of fibroblasts in 1D environments by regulating integrin clustering and focal adhesion dynamics.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Mai Al-Mohanna, Noura N. Alraouji, Samiah A. Alhabardi, Falah Al-Mohanna, Basem Al-Otaibi, Ibrahim Al-Jammaz, Abdelilah Aboussekhra
Summary: This study evaluated the therapeutic efficacy of a piperidone analogue of curcumin (PAC) for anaplastic thyroid carcinoma (ATC). PAC induced apoptosis in ATC cells through the mitochondrial pathway. It also inhibited the epithelial-to-mesenchymal transition (EMT) process and suppressed the AKT/mTOR pathway in ATC cells. In addition, PAC showed promising anti-tumor effects in vivo. Overall, PAC could be a potential therapeutic agent for ATC.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Taichi Matsumoto, Junichi Matsumoto, Yuka Matsushita, Moeno Arimura, Kentaro Aono, Mikiko Aoki, Kazuki Terada, Masayoshi Mori, Yutaka Haramaki, Takuya Imatoh, Atsushi Yamauchi, Keisuke Migita
Summary: This study investigated the effect of bortezomib (BTZ) on vascular permeability and expression of cell-cell junction molecules in human pulmonary microvascular endothelial cells. The findings showed that clinically relevant concentrations of BTZ increased the permeability of endothelial cell monolayers and downregulated the expression of claudin-5, occludin, and VE-cadherin at both protein and mRNA levels. This suggests that BTZ-induced pulmonary toxicity may be mediated by increased vascular permeability caused by the downregulation of these cell-cell junction molecules.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Jiang Liu, Junchao Zhang, Xun Fu, Shu Yang, Yan Li, Jianmin Liu, Michael E. DiSanto, Ping Chen, Xinhua Zhang
Summary: Benign prostatic hyperplasia (BPH) is a common disease in elderly men, characterized by prostatic enlargement and lower urinary tract symptoms (LUTs). Pathological processes of prostate hyperplasia are related to cell proliferation and apoptosis imbalance, inflammation, epithelial-mesenchymal transition (EMT) and growth factors. Cell adhesion molecules (CAMs), such as integrins and cadherins, play important roles in both physiological and pathological processes of BPH. Targeting CAMs may have potential therapeutic value for BPH treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Lurong Ding, Hao Ding, Ping Zhou, Lingling Xi, Bin Su
Summary: This work presents a surface-sensitive imaging methodology for evaluating cell-matrix adhesion differences at the single cell level and analyzing the impact of the chemical microenvironment on cell behaviors. The study reveals that different surface modifications lead to varying strengths of cell-matrix adhesion, and specific integrin subunits exhibit selective recognition to different surfaces.
ANALYTICAL CHEMISTRY
(2022)
Review
Clinical Neurology
Oday Atallah, Amr Badary, Yasser F. Almealawy, Vivek Sanker, Wireko Andrew Awuah, Toufik Abdul-Rahman, Sura N. Alrubaye, Bipin Chaurasia
Summary: This study investigates unexpected deaths resulting from primary brain tumors and analyzes the contributing variables. The findings reveal that unexpected deaths are a complex phenomenon, with headache being the most common initial symptom and meningiomas and astrocytomas being the most common lesions.
JOURNAL OF CLINICAL NEUROSCIENCE
(2024)
Article
Clinical Neurology
Alexandra E. Quimby, Mandy K. Salmon, Christopher H. Zhao, John Y. K. Lee, Douglas C. Bigelow, Michael J. Ruckenstein, Jason A. Brant
Summary: The study found that socioeconomic factors including race, health insurance, and income have an impact on the quality of life at the time of vestibular schwannoma diagnosis. Black/African American and uninsured/self-pay patients had lower quality of life, while patients with higher income had higher quality of life.
JOURNAL OF CLINICAL NEUROSCIENCE
(2024)
Review
Clinical Neurology
Arosha S. Dissanayake, Kwok M. Ho, Timothy J. Phillips, Stephen Honeybul, Graeme J. Hankey
Summary: This study systematically reviews models that aim to provide patient-specific predictions of pre-treatment rebleeding risk in patients with aneurysmal subarachnoid hemorrhage. The reported discriminative performance of the models varied, and no model showed consistently low bias risk and clinical applicability in all domains. Only one model was formulated using a patient cohort that underwent contemporary, evidence-based aneurysm treatment practices, but this model lacked calibration or clinical utility.
JOURNAL OF CLINICAL NEUROSCIENCE
(2024)
Article
Clinical Neurology
Colin Kok Ann Teo, Yilong Zheng, Jeremy Bingyuan Lin, Hock Luen Teoh, Bernard Poon Lap Chan, Vijay Kumar Sharma, Kejia Teo, Vincent Diong Weng Nga, Tseng Tsai Yeo
Summary: This study retrospectively reviewed the clinical characteristics and outcomes after surgical revascularization for adult Moyamoya disease (MMD) in a Southeast Asian cohort. The incidence of postoperative transient ischemic attack (TIA)/stroke was 25.9%, with most cases occurring within 7 days postoperatively. Risk factors for 30-day postoperative TIA/stroke included a higher number of preoperative TIAs/strokes and indirect revascularization.
JOURNAL OF CLINICAL NEUROSCIENCE
(2024)
Article
Clinical Neurology
Cheng-Chi Lee, Abel Po-Hao Huang, Ching-Chang Chen, Zhuo-Hao Liu, Mun-Chun Yeap, Ko-Ting Chen, Peng-Wei Hsu, Kuo-Chen Wei, Chun-Ting Chen, Yu-Chi Wang, Ting-Wei Chang, Chi-Cheng Chuang
Summary: Using a novel portable neuroendoscopic system for minimally invasive evacuation of intracerebral hemorrhage (ICH) has shown positive outcomes in reducing hematoma volume and improving neurological function without any death or rebleeding incidents.
JOURNAL OF CLINICAL NEUROSCIENCE
(2024)
Article
Clinical Neurology
Michael M. Haglund, Bruce M. McCormack, Daniel M. Williams, Alexander C. Lemons, Erik M. Summerside
Summary: This study evaluated the long-term radiographic outcomes of patients receiving tissue-sparing posterior cervical fusion (PCF) for the treatment of pseudarthrosis. The results showed that patients achieved rates of arthrodesis similar to open PCF, with fewer postoperative complications and long-term soft tissue pain.
JOURNAL OF CLINICAL NEUROSCIENCE
(2024)
Article
Clinical Neurology
Dong-Dong Meng, Zhe Ruan, Yong-Lan Tang, Zhao-Hua Ji, Yue Su, Tuo Xu, Bo-Zhou Cui, Da-Lin Ren, Ting Chang, Qian Yang
Summary: Depression symptom level, MG severity classification and family's monthly per capita income are independent factors related to the caregivers' disease family burden for MG patients. The daily activity burden of the family and the economic burden of the family were the heaviest among the six dimensions of the caregivers' family disease burdens.
JOURNAL OF CLINICAL NEUROSCIENCE
(2024)
Article
Clinical Neurology
Tugba Ozudogru Celik, Umit Gorgulu, Safiye Gul Kenar, Nadide Koca, Elif Yalcin, Ipek Koymen, Evren Yasar
Summary: This study investigated the forward head posture (FHP), thoracic kyphosis, and their relationship in individuals with migraine compared to healthy controls. The results showed that patients with migraine had a greater FHP and thoracic kyphosis. This suggests that a 3-dimensional objective measurement could be a reliable tool for evaluating posture analysis in clinical practice.
JOURNAL OF CLINICAL NEUROSCIENCE
(2024)
Article
Clinical Neurology
Jason A. Chen, Ari D. Kappel, Erickson F. Torio, David I. Bass, Abdullah Feroze, Nirav J. Patel
Summary: This report presents a case of a 63-year-old woman with a ruptured giant ICA terminus aneurysm, who underwent coil embolization followed by parent vessel sacrifice and high-flow bypass.
JOURNAL OF CLINICAL NEUROSCIENCE
(2024)
Article
Clinical Neurology
Sandeep Kandregula, Sneha Sai Mannam, Shahbaz Saad, Saarang Patel, Visish M. Srinivasan
Summary: A 39-year-old female with a history of smoking and a family predisposition to unruptured aneurysms presented with clinical symptoms of intermittent right-sided headaches, flashes of light, and pulsatile tinnitus. Diagnostic evaluations identified a right occipital arteriovenous malformation (AVM). Treatment involved partial embolization of primary arterial feeders followed by surgical resection, resulting in a successful outcome.
JOURNAL OF CLINICAL NEUROSCIENCE
(2024)
Article
Clinical Neurology
Ari D. Kappel, Jason A. Chen, Joshua I. Chalif, David I. Bass, Erickson F. Torio, Abdullah H. Feroze, Nirav J. Patel
Summary: A 48-year-old male with progressive congestive myelopathy underwent surgical clipping to treat a craniocervical DAVF. Intraoperatively, ICG was used to confirm the solitary inflow.
JOURNAL OF CLINICAL NEUROSCIENCE
(2024)
Article
Clinical Neurology
Aryeh Zolin, Cenai Zhang, Hwai Ooi, Harini Sarva, Hooman Kamel, Neal S. Parikh
Summary: In people with Parkinson's disease, comorbid liver fibrosis is associated with more rapid cognitive decline across multiple domains.
JOURNAL OF CLINICAL NEUROSCIENCE
(2024)