Article
Biology
Chen Xu, Chenyu Liu, Kaichao Chen, Ping Zeng, Edward Wai Chi Chan, Sheng Chen
Summary: The drug otilonium bromide restores the activity of colistin against colistin-resistant Gram-negative bacteria and reduces toxicity, it also exhibits synergistic effect in eradicating multidrug-tolerant persisters of Gram-negative bacteria.
COMMUNICATIONS BIOLOGY
(2022)
Article
Ecology
Thomas James O'Brien, Wendy Figueroa, Martin Welch
Summary: The presence of diverse polymicrobial communities in the airways of individuals with cystic fibrosis can hinder the effectiveness of antibiotic treatment. A study showed that growth in a polymicrobial environment protects the target microorganism from the effects of specific antimicrobial agents. This decreased efficacy is found to have both physiological and genetic components.
Article
Microbiology
Luisa Sorli, Sonia Luque, Jian Li, Adela Benitez-Cano, Xenia Fernandez, Nuria Prim, Victoria Vega, Joan Gomez-Junyent, Inmaculada Lopez-Montesinos, Silvia Gomez-Zorrilla, M. Milagro Montero, Santiago Grau, Juan Pablo Horcajada
Summary: This study investigated the relationship between colistin plasma concentrations and clinical outcomes in patients with hospital-acquired pneumonia caused by extensively drug-resistant Pseudomonas aeruginosa. The findings showed that higher colistin exposure did not improve clinical outcomes.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Lingbo Wang, Ying Zhang, Yishuai Lin, Jianming Cao, Chunyan Xu, Liqiong Chen, Yaran Wang, Yao Sun, Xiangkuo Zheng, Yong Liu, Tieli Zhou
Summary: P. aeruginosa is a widespread bacterium associated with life-threatening infections. Improper use of antibiotics has led to the emergence of multidrug-resistant strains, including colistin-resistant strains. This study demonstrates that colistin/resveratrol combination therapy has a strong synergistic effect in eradicating colistin-resistant P. aeruginosa strains and their biofilms.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Tomasz Bogiel, Malgorzata Prazynska, Joanna Kwiecinska-Pirog, Agnieszka Mikucka, Eugenia Gospodarek-Komkowska
Summary: The study compared the frequency of selected enzymatic virulence factor genes among non-duplicated carbapenem-resistant P. aeruginosa isolates, finding a higher prevalence of alkaline protease gene and a 37.4% detection rate for neuraminidase-1 gene. Significant differences in the prevalence of lasB and nan1 were observed between MBL-positive and MBL-negative strains.
Article
Pharmacology & Pharmacy
Sandra Patricia Rivera-Sanchez, Ivan Dario Ocampo-Ibanez, Yamil Liscano, Natalia Martinez, Isamar Munoz, Marcela Manrique-Moreno, Luis Martinez-Martinez, Jose Onate-Garzon
Summary: This study investigates the interaction between a cationic antimicrobial peptide (CAMP) derived from Cecropin D-like and bacterial-like model membranes of colistin-resistant Pseudomonas aeruginosa (CRPa) strains. The experimental analysis shows that the peptide interacts with the lipids of CRPa membranes, resulting in an increase in the phase transition temperature of the lipid systems and a transition in the peptide's secondary structure.
Review
Microbiology
Amber Grace, Rajnish Sahu, Donald R. R. Owen, Vida A. A. Dennis
Summary: Pseudomonas aeruginosa is a common pathogen that has been identified as a multi-drug resistant strain. PAO1 and PA14, two laboratory strains, have contributed significantly to our understanding of the bacterium. However, there are discrepancies between their behaviors and therapeutic susceptibilities observed in human infections. This review aims to compare the two strains and propose methods to improve their clinical relevance.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Physical
Xin Zhou, Dirk Fennema Galparsoro, Anders Ostergaard Madsen, Valeria Vetri, Marco van de Weert, Hanne Morck Nielsen, Vito Fodera
Summary: Amyloid protein aggregates are associated with neurodegenerative diseases and can occur as by-products in protein-based therapeutics. The use of surfactants to stabilize protein formulations can affect protein-protein interactions and alter the properties of the aggregates formed.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Microbiology
Saoirse Disney-McKeethen, Seokju Seo, Heer Mehta, Karukriti Ghosh, Yousif Shamoo
Summary: Antibiotic resistance is a global health crisis, and understanding the evolutionary trajectories leading to increased resistance is crucial for developing clinical diagnostics and discovering new drug targets. Researchers found that adaptive mutations to phoQ were more successful than pmrB in increasing resistance to colistin, a commonly used antibiotic. The study also revealed that the oxygen concentration gradients within the microdroplet emulsions favored changes to the PhoP/PhoQ pathway, which is consistent with the microaerobic conditions found in the lungs of cystic fibrosis patients.
Article
Microbiology
Jingchun Kong, Yue Wang, Zhuocheng Yao, Yishuai Lin, Yi Zhang, Yijia Han, Tieli Zhou, Jianzhong Ye, Jianming Cao
Summary: In this study, the combination of colistin and eugenol was found to have a significant synergistic antibacterial effect against colistin-resistant Pseudomonas aeruginosa and Klebsiella pneumoniae. The combination increased membrane permeability and caused membrane damage, inhibiting bacteria synergistically. Eugenol was non-toxic to cells and the combination was effective in vivo. These findings suggest that the colistin/eugenol combination may be a viable treatment option for colistin-resistant bacterial infections.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Yuhang Wang, Chunsun Li, Jin Wang, Nan Bai, Huan Zhang, Yulong Chi, Yun Cai
Summary: In this study, the in vitro and in vivo efficacy of colistin-based combinations against Pseudomonas aeruginosa (PA) biofilm were evaluated. The results showed that colistin combined with levofloxacin or amikacin could effectively eradicate the biofilm. This study provided a new treatment regimen for PA biofilm infection.
MICROBIOLOGY SPECTRUM
(2022)
Article
Chemistry, Medicinal
Zinan Zhang, Dominic Ortega, Anthony Rush, Lauren R. Blankenship, Zi Jun Cheng, Rebecca E. Moore, Minh L. N. Tran, Lucero G. Sandoval, Kareem Aboulhosn, Seiichiro Watanabe, Kendra S. Cortez, David H. Perlman, Martin F. Semmelhack, Laura C. Miller Conrad
Summary: Newly developed antipyocyanin compounds not only have potential antivirulence effects, but may also act as antibiotic adjuvants to amplify bactericidal activity. These compounds could represent a first-in-class antibiotic adjuvant therapy, widening the therapeutic index and strengthening our arsenal against Pseudomonas aeruginosa infections.
ACS INFECTIOUS DISEASES
(2021)
Article
Chemistry, Applied
Shigekazu Yano, Senri Terada, Misaki Fukushi, Yuitsu Otsuka, Satomi Mizunuma, Satoshi Asakura, Takahiro Kawai, Hiroyuki Konno, Masatoshi Endo, Yoshimune Nonomura
Summary: The study investigated the properties of polysorbate 80-iodine complex and found that high concentrations of polysorbate 80 enhanced the antibacterial activity of iodine. The complex can retain low concentrations of iodine and be stably stored for a long time.
JOURNAL OF OLEO SCIENCE
(2022)
Article
Microbiology
Alexandra J. J. Lee, Georgia Doing, Samuel L. L. Neff, Taylor Reiter, Deborah A. A. Hogan, Casey S. S. Greene
Summary: This article studies the genomic heterogeneity of Pseudomonas aeruginosa strains, compares the expression patterns of core genes and accessory genes between PAO1 and PA14 strains, and finds differences in the expression patterns of some core genes among different strains.
Article
Infectious Diseases
Jessica Howard-Anderson, Michelle Davis, Alexander M. Page, Chris W. Bower, Gillian Smith, Jesse T. Jacob, Dan Andersson, David S. Weiss, Sarah W. Satola
Summary: This study investigated the prevalence of colistin heteroresistance in carbapenem-resistant Pseudomonas aeruginosa (CRPA) and its association with clinical outcomes. The results showed that colistin heteroresistance was present in up to 26% of CRPA patients, but it did not have a significant impact on 90-day mortality.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
