Article
Microbiology
Merili Saar, Risto Vaikjarv, Ulle Parm, Priit Kasenomm, Siiri Koljalg, Epp Sepp, Madis Jaagura, Andres Salumets, Jelena Stsepetova, Reet Mandar
Summary: Based on next generation sequencing analysis, the most probable causative agents of peritonsillar abscess (PTA) include Streptococcus pyogenes, Fusobacterium necrophorum, and Fusobacterium nucleatum. Other streptococci and anaerobes may also contribute to the infection. Pus samples are more representative for microbiological examination than tonsil biopsies. These findings are important for optimizing the management of PTA patients.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2023)
Article
Multidisciplinary Sciences
Kile S. Townsend, Philip J. Johnson, Alison M. LaCarrubba, Lynn M. Martin, Aaron C. Ericsson
Summary: Bacteremia resulting from dental surgery poses a health risk to older and immunocompromised patients. Oral cavity commensals may play a significant role in the pathogenesis of various diseases. Bacteremia associated with translocated gingival commensals can occur during dental extraction procedures.
SCIENTIFIC REPORTS
(2021)
Article
Pharmacology & Pharmacy
Zhongkun Zhou, Yiqing Wang, Rui Ji, Dekui Zhang, Chi Ma, Wantong Ma, Yunhao Ma, Xinrong Jiang, Kangjia Du, Rentao Zhang, Peng Chen
Summary: This study reveals that the abundance of F. nucleatum is higher in CRC patients in Northwest China. The vanillin derivatives IPM711 and IPM712 show better anti-F. nucleatum activity by increasing cell membrane permeability and destroying bacterial integrity. Additionally, IPM711 and IPM712 can suppress the migration of HCT116.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tian Zhao, Jiaqi Chen, Shuai Liu, Jie Yang, Juan Wu, Leiying Miao, Weibin Sun
Summary: Periodontitis is a chronic infectious disease that can lead to various dental problems and even tooth loss. The dental plaque biofilm is a crucial factor in the development of periodontitis. Fusobacterium nucleatum plays an important role in the structure and ecology of dental plaque biofilms. Understanding the molecular mechanism of F. nucleatum during biofilm development is essential for controlling periodontitis. This study used RNA-sequencing and quantitative reverse transcriptase PCR (RT-qPCR) to analyze the gene expression profiles of F. nucleatum in the planktonic and biofilm phases. The results revealed significant differences in gene expression between the two states, with several genes related to metabolism, cell growth, division, oxidative stress, virulence, and oral malodor being differentially expressed. Additionally, the study identified lysine as a potential antibacterial and antibiofilm agent against F. nucleatum.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Medicine, General & Internal
Weiteng Wang, Oudi Chen, Weijiang Liu, Lixi Gan, Xin Li, Qingyan Ma, Xuejiao Hu, Xuhua Jian
Summary: Metagenomic next-generation sequencing successfully identified culture-negative endocarditis caused by Coxiella burnetii and Bartonella. The cardiac valvular tissue samples showed higher suspected pathogen reads than blood specimens. Treatment based on etiology resulted in favorable clinical outcomes, but delayed treatment led to poor prognosis.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Immunology
Ruben Dyrhovden, Tomas Mikal Eagan, Oystein Flotten, William Siljan, Truls Michael Leegaard, Bjornar Bo, Hilde Fardal, Fredrik Grovan, Arne Kildahl-Andersen, Kjersti Wik Larssen, Rune Tilseth, Reidar Hjetland, Sigbjorn Loes, Frode Lindemark, Marit Tellevik, Rebecca Breistein, Oyvind Kommedal
Summary: This study provides a comprehensive microbiological and clinical characterization of oral-type pleural infections and highlights the importance of hematogenous seeding from dental infections as the underlying etiology.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Immunology
Yueming Liang, Tingyan Dong, Minjing Li, Peifang Zhang, Xiaoqun Wei, Haitao Chen, Yongsi Wang, Xinglin Gao
Summary: The incidence of severe Chlamydia psittaci pneumonia and coinfections is increasing. Early detection using metagenomic next-generation sequencing (mNGS) can accurately detect C. psittaci and other pathogens, as well as identify coinfections for better clinical treatment.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Muhammad Tanweer Khan, Chinmay Dwibedi, Daniel Sundh, Meenakshi Pradhan, Jamie D. Kraft, Robert Caesar, Valentina Tremaroli, Mattias Lorentzon, Fredrik Backhed
Summary: This study successfully co-isolated Faecalibacterium prausnitzii and Desulfovibrio piger and identified their symbiotic relationship in growth and butyrate production. By adapting Faecalibacterium prausnitzii to tolerate oxygen exposure, researchers developed a next-generation probiotic formulation.
Article
Immunology
Chengxin Liu, Juhua Yang, Huiting Huang, Shaofeng Zhan, Xintian Xia
Summary: Infection of Nocardia gipuzkoensis is a relatively uncommon form of pulmonary nocardiosis, usually occurring in patients with immune deficiency. We report a case of a 23-year-old female with a 10-year history of cough and sputum production, diagnosed with bronchiectasis. The infection was identified using metagenomic next-generation sequencing and whole genome sequencing, and was controlled with appropriate medication.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Shuwei Zhang, Chen Li, Zhiying Zhang, Yuchao Li, Qian Li, Fengxue Geng, Junchao Liu, Yaping Pan
Summary: This study identified differential expression of 3307 mRNAs and 522 lncRNAs in HIOECs infected with F. nucleatum, and investigated the potential functions of these dysregulated genes through GO and KEGG pathway analyses. The study identified top 10 hub genes involved in malignant transformation through protein-protein interaction network, and generated a co-expression network of dysregulated lncRNAs associated with hub genes.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Medicine, General & Internal
Junyan Qu, Zhiyong Zong
Summary: This case report presents a case of strongyloidiasis in a Chinese male patient diagnosed using metagenomic next-generation sequencing (mNGS). The study highlights the potential of mNGS in detecting uncommon infectious diseases and emphasizes the importance of raising awareness of strongyloidiasis in non-endemic areas.
FRONTIERS IN MEDICINE
(2022)
Article
Public, Environmental & Occupational Health
Jie Huang, Liming Wang, Yumei Xu, Xinhua Hu, Ronghuan Yu, Shi Chen, Baoqing Wang
Summary: This study reported the first case of E. halliae infection in China, emphasizing the importance and accuracy of mNGS in bacterial diagnosis of empyema.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Public, Environmental & Occupational Health
Junyan Qu, Huan Xu, Xiaoju Lv
Summary: Alveolar echinococcosis (AE) is a highly fatal and disabling parasitic infection with diverse clinical manifestations and difficulties in differential diagnosis. Metagenomic next-generation sequencing (mNGS) has become an attractive approach for the etiological diagnosis of infectious diseases. In this case, mNGS was used to diagnose disseminated AE, which can help improve clinicians' diagnostic skills.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Cell Biology
Chia-Hui Lo, Deng-Chyang Wu, Shu-Wen Jao, Chang-Chieh Wu, Chung-Yen Lin, Chia-Hsien Chuang, Ya-Bo Lin, Chien-Hsiun Chen, Ying-Ting Chen, Jiann-Hwa Chen, Koung-Hung Hsiao, Ying-Ju Chen, Yuan-Tsong Chen, Jaw-Yuan Wang, Ling-Hui Li
Summary: This study identified the overrepresentation of Prevotella intermedia in colorectal adenocarcinoma tissues and its role in enhancing migration and invasion abilities of CRC cells. The co-abundance of P. intermedia and F. nucleatum in tumor tissues was associated with lymph node involvement and distant metastasis, with additive effects on CRC cell migration and invasion. These findings could help identify patients at high risk for malignant transformation of colorectal adenomas or metastasis of CRC for optimal clinical management.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Review
Medicine, General & Internal
Yue Sun, Han Dong, Na Zhang, Peng Zhao, Yuan Qi, Xin Yang, Lingling Wang
Summary: We reported a case of a 49-year-old man with a 1-year history of intermittent cough and sputum production who had worsened over the last 4 days with fever and right chest pain. Fusobacterium nucleatum was detected in the pleural effusion by using next-generation sequencing. A diagnosis of squamous cell carcinoma of the right lung was made by fiberoptic bronchoscopy. The patient's condition improved significantly after percutaneous drainage and long-term intravenous antibiotic treatment.
FRONTIERS IN MEDICINE
(2023)
