Article
Infectious Diseases
Antonella Santona, Jose Joao Sumbana, Maura Fiamma, Massimo Deligios, Elisa Taviani, Samuel Elija Simbine, Tomas Zimba, Jahit Sacarlal, Salvatore Rubino, Bianca Paglietti
Summary: ESBL-producing ExPEC, including high-risk lineages, were identified in extraintestinal infections at Maputo Central Hospital. The majority of isolates showed multidrug resistance, with ESBL genes associated with resistance to cefotaxime and ceftazidime. Furthermore, different sequence types were found to carry CTX-M transposition units, potentially facilitating the dissemination of ESBL genes. Genomic analysis revealed a diverse array of virulence-associated genes and clonality among isolates, suggesting the presence of hospital-acquired infections and long-term persistence.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Infectious Diseases
Antonio Mandujano, Diana Veronica Cortes-Espinosa, Jose Vasquez-Villanueva, Paulina Guel, Gildardo Rivera, Karina Juarez-Rendon, Wendy Lizeth Cruz-Pulido, Guadalupe Aguilera-Arreola, Abraham Guerrero, Virgilio Bocanegra-Garcia, Ana Veronica Martinez-Vazquez
Summary: This study investigated the antimicrobial resistance profile of ESBL-producing E. coli among food-producing animals in Tamaulipas, Mexico. A total of 200 fecal samples were collected and 5.0% of the strains were confirmed as ESBL producers. High percentage of antimicrobial resistance was observed against gentamicin, tetracycline, and streptomycin. The study suggests that food-producing animals may serve as reservoirs and contribute to the spread of ESBL-producing bacteria.
Article
Infectious Diseases
Jiayu Shao, Hangdong Dai, Liwei Xu, Shuilong Zhu, Jufang Zhu, Hangyu Fu, Minxia Ge, Xianhong He
Summary: The purpose of this study was to investigate the genomic characteristics and antimicrobial resistance mechanisms of ESBL-producing E. coli isolates recovered from a district hospital in China. The results showed that these isolates exhibited resistance to multiple antibiotics, with the majority belonging to the ST131 serotype and having different virulence genes and sequence types. Therefore, continuous surveillance and control of the transmission of these multi-drug resistant bacteria in clinical and community settings are crucial.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Microbiology
Wietske Dohmen, Apostolos Liakopoulos, Marc J. M. Bonten, Dik J. Mevius, Dick J. J. Heederik
Summary: A long-term study in 39 Dutch pig farms revealed the presence of ESBL-producing Enterobacterales in both pigs and pig farmers, suggesting the possibility of genetic and horizontal transmission. These findings are crucial for developing targeted intervention strategies to reduce the risk of human exposure to ESBL-producing Enterobacterales.
MICROBIOLOGY SPECTRUM
(2023)
Article
Engineering, Environmental
Aradhana Basu, Ananyo Jyoti Misra, Meerambika Behera, Susanta Kumar Behera, Ashish Kumar Nayak, Nabin Kumar Dhal, Amrita Mishra, Bijoy Kumar Satpathy, Cecilia Stalsby Lundborg, Suraj K. Tripathy
Summary: The study demonstrates successful photocatalytic disinfection of MDR Escherichia coli using Alumina/ZnO heterostructures. The proposed technique shows efficacy in disinfecting various bacteria and exhibits biocompatibility towards animal models.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Infectious Diseases
Hyunsoo Kim, Young Ah Kim, Young Hee Seo, Hyukmin Lee, Kyungwon Lee
Summary: This study aimed to investigate the molecular epidemiology of extended-spectrum-beta-lactamase producing Escherichia coli (ESBL-EC) from poultry, the poultry farm environment, and workers in Korea. The study found that the isolation rates of ESBL-EC were not negligible in the poultry industry-related samples, sharing common ESBL types of human ESBL-EC isolates in Korea. Whole genome sequencing revealed numerous antimicrobial resistance genes and well-known antimicrobial gene-carrying plasmids in the ESBL-EC isolates.
Article
Medicine, General & Internal
Monica Romero Nieto, Sara Maestre Verdu, Vicente Gil, Carlos Perez Barba, Jose Antonio Quesada Rico, Reyes Pascual Perez
Summary: This study identified male sex, smoking, hypertension, urinary incontinence, and recurrent urinary tract infections as independent factors associated with the presence of ESBL-producing E. coli in patients with acute community-acquired pyelonephritis. The multivariate logistic models could correctly classify individuals with ESBL-producing E. coli infection in 80.2% of cases.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Veterinary Sciences
Dennis J. Woerde, Krystle L. Reagan, Barbara A. Byrne, Bart C. Weimer, Steven E. Epstein, Cory Schlesener, Bihua C. Huang, Jane E. Sykes
Summary: This study examined ESBL-producing bacterial infections in dogs and cats presenting to a veterinary teaching hospital from 2011-2021. Escherichia coli was the most commonly identified bacterial species, with urinary tract infection being the most common clinical presentation. Multi-drug resistance was present in 90% of ESBL-producing bacterial infections. Based on susceptibility patterns, antimicrobials such as piperacillin-tazobactam, amikacin, and cefoxitin may be alternative antibiotics to the current recommended regimen. Whole genome sequencing of bacteria revealed bla(CTX-M-15) as the most common ESBL gene.
VETERINARY SCIENCES
(2023)
Article
Microbiology
Anna R. Holtmann, Diana Meemken, Anja Mueller, Diana Seinige, Kathrin Buettner, Klaus Failing, Corinna Kehrenberg
Summary: This study revealed the presence of ESBL-/AmpC-E. coli in wild boars, with a significant positive association with human population density. The E. coli isolates carried various β-lactamase genes and showed multiple drug resistances.
Article
Immunology
Mabel Kamweli Aworh, Eme Ekeng, Pernille Nilsson, Beverly Egyir, Christian Owusu-Nyantakyi, Rene S. Hendriksen
Summary: This study investigated the zoonotic transmission of extended-spectrum beta-lactamase-producing E. coli (ESBL-EC) among humans, beef cattle, and abattoir environments in Nigeria. The study found a high prevalence of ESBL-EC in beef cattle and abattoir workers, highlighting the importance of genomic surveillance and raising awareness among healthcare workers.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Mitul A. Patel, Aparna Pandey, A. C. Patel, S. S. Patel, H. C. Chauhan, M. D. Shrimali, Pankaj A. Patel, S. K. Mohapatra, B. S. Chandel
Summary: This study revealed the molecular characteristics and high occurrence of ESBL E. coli in poultry in Gujarat, India, mainly attributed to the genes bla(TEM), bla(SHV), and bla(OXA).
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
F. Gravey, S. Wilson, A. Denisenko, C. Lehoussel, Marguerite Fines-Guyon, S. Le Hello, C. Jeanne-Leroyer
Summary: Whole genome sequencing is now the standard method used for microbiological investigations, enabling the detection of undisclosed outbreaks. By utilizing this approach, we were able to investigate and ultimately halt an uncommon epidemic caused by the extended-spectrum beta-lactamase-producing Klebsiella pneumoniae ST584 strain that spanned over four months in two intensive care units.
Article
Microbiology
Heather M. M. Blankenship, Stephen E. E. Dietrich, Elizabeth Burgess, Jason Wholehan, Marty Soehnlen, Shannon D. D. Manning
Summary: This study conducted a retrospective analysis of 510 clinical STEC isolates using core genome SNP analysis and found that PFGE and MLST could not fully reveal the genetic diversity and relatedness. SNP analysis was able to cluster isolates based on similarity in PFGE and MLST and uncover their phylogenetic relationships. The use of high-quality SNP analysis enabled a more rapid identification of related strains during outbreak investigations. This study demonstrates how public health laboratories can utilize whole-genome sequencing and phylogenetics to identify related strains and uncover important genetic attributes that can guide treatment practices.
Article
Infectious Diseases
Jerome Ambroise, Elmostafa Benaissa, Leonid Mwana Wa Bene Irenge, El Mehdi Belouad, Bertrand Bearzatto, Jean-Francois Durant, Jamal Badir, Mostafa Elouennass, Jean-Luc Gala
Summary: This study aimed to provide data on ESBL-producing E. coli isolates from patients with extraintestinal infections in Morocco. The results underscore the pre-eminence of ST131 as the major factor driving the expansion of ExPEC in the Rabat region.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Article
Microbiology
Francesca Leoni, Luca Sacchini, Silvia Pieralisi, Gabriele Angelico, Chiara Francesca Magistrali, Lucilla Cucco, Francesca Romana Massacci, Elisa Albini, Anna Duranti, Cesare Camma, Barbara Secondini, Antonio Rinaldi, Francesca Barchiesi
Summary: This study investigated the occurrence of ESBL-producing E. coli in clams collected from the Central Adriatic Sea. The results showed that approximately 3% of the samples contained ESBL-producing E. coli, with the majority of the isolates being multidrug-resistant. These findings suggest that E. coli could serve as an indicator organism for ESBL-producing E. coli in bivalves.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Jianhua Zhang, Eveline Snelders, Bas J. Zwaan, Sijmen E. Schoustra, Jacques F. Meis, Karin van Dijk, Ferry Hagen, Martha T. van der Beek, Greetje A. Kampinga, Jan Zoll, Willem J. G. Melchers, Paul E. Verweij, Alfons J. M. Debets
Meeting Abstract
Oncology
Amit M. Deshpande, Rachel Fontana, Yue Zhang, Mary Ruisi, Dong Zhang, Tilo Senger, P. Alexander Rolfe
Article
Multidisciplinary Sciences
Matthias Schroeder, Marit Kroetschel, Lena Conrad, Svenja Kerstin Naumann, Christopher Bachran, Alex Rolfe, Viktor Umansky, Laura Heming, Lee Kim Swee
SCIENTIFIC REPORTS
(2018)
Review
Pharmacology & Pharmacy
Vijayalakshmi Chelliah, Georgia Lazarou, Sumit Bhatnagar, John P. Gibbs, Marjoleen Nijsen, Avijit Ray, Brian Stoll, R. Adam Thompson, Abhishek Gulati, Serguei Soukharev, Akihiro Yamada, Jared Weddell, Hiroyuki Sayama, Masayo Oishi, Sabine Wittemer-Rump, Chirag Patel, Christoph Niederalt, Rolf Burghaus, Christian Scheerans, Joerg Lippert, Senthil Kabilan, Irina Kareva, Natalya Belousova, Alex Rolfe, Anup Zutshi, Marylore Chenel, Filippo Venezia, Sylvain Fouliard, Heike Oberwittler, Alix Scholer-Dahirel, Helene Lelievre, Dean Bottino, Sabrina C. Collins, Hoa Q. Nguyen, Haiqing Wang, Tomoki Yoneyama, Andy Z. X. Zhu, Piet H. van der Graaf, Andrzej M. Kierzek
Summary: Drug development in oncology often uses molecular biology tools to reprogram cellular responses for therapeutic benefits. In immuno-oncology, the goal is to mobilize the patient's immune system against cancer. While successful with antibodies targeting specific receptors, the trend has shifted towards combination therapies, which presents challenges in efficiency and sustainability.
CLINICAL PHARMACOLOGY & THERAPEUTICS
(2021)
Article
Oncology
Julius Strauss, Margaret E. Gatti-Mays, Byoung Chul Cho, Andrew Hill, Sebastien Salas, Edward McClay, Jason M. Redman, Houssein A. Sater, Renee N. Donahue, Caroline Jochems, Elizabeth Lamping, Andrea Burmeister, Jennifer L. Marte, Lisa M. Cordes, Marijo Bilusic, Fatima Karzai, Laureen S. Ojalvo, Genevieve Jehl, P. Alexander Rolfe, Christian S. Hinrichs, Ravi A. Madan, Jeffrey Schlom, James L. Gulley
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2020)
Article
Oncology
Elisabeth Strack, P. Alexander Rolfe, Annika F. Fink, Katrin Bankov, Tobias Schmid, Christine Solbach, Rajkumar Savai, Weixiao Sha, Leon Pradel, Sylvia Hartmann, Bernhard Bruene, Andreas Weigert
CLINICAL AND TRANSLATIONAL MEDICINE
(2020)
Article
Immunology
Eveline Snelders, Frederique Moyrand, Aude Sturny-Leclere, Frederique Vernel-Pauillac, Stevenn Volant, Guilhem Janbon, Alexandre Alanio
Summary: It is apparent that GPI-anchored proteins (GAPs) play a significant role in fungi, but the understanding of GAPs in Cryptococcus neoformans, a human opportunistic fungus, is still limited. By utilizing online GPI prediction tools, GAPs were identified and followed by the development of a mutant library for these GAP-encoding genes. A publicly available knock out (KO) mutant library was employed. A total of 41 overexpression and 34 KO mutants representing 47 unique genes were analyzed. Among the screened genes, two candidates, mannoprotein 88 (MP88) (CNAG_00776) and an uncharacterized protein (CNAG_00137), were further investigated by constructing additional independent mutant strains. The CNAG_00776 mutant displayed impaired growth under plasma membrane stress and significantly reduced phagocytosis. The CNAG_00137 mutant exhibited impaired growth during cell wall stress or increased temperature and a significant decrease in phagocytosis. Through a comprehensive genetic screen of GAPs in the genome of C. neoformans, two potential GAP genes involved in C. neoformans/host interaction and stress response were identified. Further exploration of these genes could lead to the discovery of new targets for antifungal drugs, treatment strategies, or vaccines to manage C. neoformans disease. (C) 2022 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
MICROBES AND INFECTION
(2022)
Article
Medicine, Research & Experimental
Yulia Vugmeyster, George Locke, Christoph Helwig, P. Alexander Rolfe, Jennifer Q. Dong, Karthik Venkatakrishnan
Summary: The study evaluated the drug-drug interaction potential of bintrafusp alfa and found it to have low risk of modulation on the CYP3A4 enzyme.
CTS-CLINICAL AND TRANSLATIONAL SCIENCE
(2022)
Article
Multidisciplinary Sciences
Irina Kalatskaya, Gavin Giovannoni, Thomas Leist, Joseph Cerra, Ursula Boschert, P. Alexander Rolfe
Summary: Deconvolution methods based on gene expression profiling are an attractive alternative to flow cytometry for quantifying immune cells. This study investigated the applicability of these methods in clinical trials to better understand the mode of action of drugs for autoimmune diseases. The popular deconvolution methods CIBERSORT and xCell were validated using gene expression data and flow cytometry results. The results showed changes in the immune cell profile of relapsing multiple sclerosis patients treated with cladribine tablets, confirming previously described changes and revealing potential long-term efficacy.
SCIENTIFIC REPORTS
(2023)
Article
Respiratory System
Tobias Engel, Paul E. Verweij, Joost van den Heuvel, Dechen Wangmo, Jianhua Zhang, Alfons J. M. Debets, Eveline Snelders
Article
Medicine, General & Internal
Christian Ihling, Bartholomew Naughton, Yue Zhang, P. Alexander Rolfe, Eveline Frick-Krieger, Luigi M. Terracciano, Isabelle Dussault
FRONTIERS IN MEDICINE
(2019)
Article
Oncology
Chunxiao Xu, Yanping Zhang, P. Alexander Rolfe, Vivian M. Hernandez, Wilson Guzman, Giorgio Kradjian, Bo Marelli, Guozhong Qin, Jin Qi, Hong Wang, Huakui Yu, Robert Tighe, Kin-Ming Lo, Jessie M. English, Laszlo Radvanyi, Yan Lan
CLINICAL CANCER RESEARCH
(2017)
Meeting Abstract
Oncology
Christian Ihling, Sienna Yoast, Yue Zhang, Bartholomew Naughton, Miriam Urban, P. Alexander Rolfe, Eveline Frick-Krieger, Isabelle Dussault
Article
Infectious Diseases
Jan Zoll, Eveline Snelders, Paul E. Verweij, Willem J. G. Melchers
CURRENT FUNGAL INFECTION REPORTS
(2016)
Article
Biotechnology & Applied Microbiology
Jianhua Zhang, Lidia Lopez Jimenez, Eveline Snelders, Alfons J. M. Debets, Anton G. Rietveld, Bas J. Zwaan, Paul E. Verweij, Sijmen E. Schoustra
Summary: This study confirmed that long-term storage sites of azole-containing decaying plant material can indeed be considered hot spots, which can sustain resistance development and maintenance in A. fumigatus. Roughly half of individual isolates were azole resistant and carried genetic mutations that are highly similar to those found in patients with azole-resistant invasive aspergillosis. Our work suggests that environmental sources of azole resistance in A. fumigatus may be important, underscoring the need for further studies on environment-to-patient transmission routes.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
