期刊
JOURNAL OF CLINICAL MICROBIOLOGY
卷 52, 期 4, 页码 1266-1268出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.00245-14
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资金
- National Natural Science Foundation of China [81071433]
- Shenzhen Public Service Platform of Pathogenic Microorganisms Repository
We developed a highly sensitive reverse transcription and multiplex real-time PCR (rtPCR) assay that can identify five viruses, including six genogroups, in a single reaction: norovirus genogroups I and II; sapovirus genogroups I, II, IV, and V; human rotavirus A; adenovirus serotypes 40 and 41; and human astrovirus. In comparison to monoplex rtPCR assays, the sensitivities and specificities of the multiplex rtPCR ranged from 75% to 100% and from 99% to 100%, respectively, evaluated on 812 clinical stool specimens.
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