Article
Chemistry, Analytical
Xiang Xiong, Wenya Dang, Ruxin Luo, Ying Long, Chunyi Tong, Liqin Yuan, Bin Liu
Summary: This study developed a versatile nanoprobe based on rGO and DNA probe for simultaneous visualization of miR451a and miR-214-3p in living cells, showing excellent selectivity and sensitivity. In vitro experiments demonstrated good linearity of detection signals for miR-451a and miR-214-3p, and successful dual-color imaging in human breast cancer cells was achieved using the nanoprobe.
Article
Food Science & Technology
Caroline Puente-Lelievre, Anne C. Eischeid
Summary: The study developed a novel real-time PCR assay targeting two chloroplast regions using LNA probes to specifically detect walnut in foods. By spiking food matrices with walnut at different concentrations and achieving high reaction efficiencies, the assay proved to be an effective tool for allergenic walnut detection.
Article
Oncology
Hsiao-Wen Kao, Ming-Chung Kuo, Ying-Jung Huang, Hung Chang, Shu-Fen Hu, Chein-Fuang Huang, Yu-Shin Hung, Tung-Liang Lin, Che-Wei Ou, Ming-Yu Lien, Jin-Hou Wu, Chih-Cheng Chen, Lee-Yung Shih
Summary: The study demonstrates the potential of LNA-qPCR for monitoring IDH1/2 mutations MRD in AML by correlating with NPM1 qPCR MRD and predicting relapse risk.
Article
Chemistry, Analytical
Hisaaki Hirose, Eiji Nakata, Zhengxiao Zhang, Yuya Shibano, Masashi Maekawa, Takashi Morii, Shiroh Futaki
Summary: This study developed a fluorescent sensor that can simultaneously analyze environmental factors in a limited cellular space, allowing for precise understanding of molecular interactions and biological responses in live cells. The sensor was able to detect both pH levels and cathepsin B activity in individual macropinosomes, providing new insights into the intracellular behaviors of macropinocytosis.
ANALYTICAL CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Fuyu Du, Weijie Zhang, Huimin Yao, Yuqiong Xia, Xianghan Zhang, Peng Yang, Pengbo Ning
Summary: A nucleic acid extraction-free and amplification-free RNA virus testing probe based on the double-stranded molecular beacon method is proposed for the sensitive and simple detection of CSFV and SARS-CoV-2. The detection limit of the probe was found to be as low as 0.28 nM and 0.24 nM for CSFV and SARS-CoV-2, respectively. The probe showed enhanced fluorescence signals in a concentration- and time-dependent manner when CSFV E2 and SARS-CoV-2 N genes were transfected into host cells. The results were supported by quantitative and visualization analyses. The proposed RNA virus probe could serve as a PCR-free, cost-effective, and rapid diagnostic platform for target RNA virus detection.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Materials Science, Biomaterials
Dong-Hui Wang, Li-Jian Chen, Xu Zhao, Xiu-Ping Yan
Summary: This study presents a novel self-reporting photosensitizer TPA-3PyA+ for photodynamic therapy and real-time monitoring, featuring dual-color emissions to self-report cell death effectively.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Medical Laboratory Technology
Jianyan Pan, Chunhua Zhang, Yanling Teng, Sijing Zeng, Siyi Chen, Desheng Liang, Zhuo Li, Lingqian Wu
Summary: A novel SMN1 detection approach using LNA-modified primers was developed, which showed enhanced specificity and efficiency compared to conventional primers. Clinical trials validated the 100% sensitivity and specificity of this new approach for detecting SMA patients and carriers. Screening results revealed a carrier rate of 1.99% in Hunan province, South Central China.
ANNALS OF LABORATORY MEDICINE
(2021)
Article
Chemistry, Multidisciplinary
Eiji Nakata, Hisaaki Hirose, Khongorzul Gerelbaatar, Jan Vincent V. Arafiles, Zhengxiao Zhang, Shiroh Futaki, Takashi Morii
Summary: Real-time monitoring of cellular environment, such as intracellular pH, in a defined cellular space provides a comprehensive understanding of dynamic processes in living cells. By assembling multiple fluorophores within a space smaller than the spatial resolution of conventional microscopy, a flexible combinatorial design strategy for developing fluorescent probes is enabled. Assembling fluorescein (CF) and tetramethylrhodamine (CR) on a DNA nanostructure with controlled numbers of each fluorophore type allows for controlled fluorescence emission intensity and ratiometric pH monitoring within the defined space.
Article
Biochemistry & Molecular Biology
Marcela S. Bucardo, You Wu, Paul T. Ludford, Yao Li, Andrea Fin, Yitzhak Tor
Summary: This study investigates two emissive heterocyclic cores as surrogate GDA substrates, demonstrating a new guanine surrogate that can be used for real-time study of GDA and its inhibition.
ACS CHEMICAL BIOLOGY
(2021)
Article
Virology
Ellen Kathrin Link, Matthias Eddicks, Liangliang Nan, Mathias Ritzmann, Gerd Sutter, Robert Fux
Summary: Genotyping of PCV2 is important for diagnosis and epidemiological studies. The new qPCR system introduced in this study is ideal for mass testing and can be a valuable complement to PCV2 sequencing, especially in cases of simultaneous infections with multiple PCV2 genotypes.
Article
Public, Environmental & Occupational Health
Ashish Parikh, Lauren Cooper, Daniel Frogel, Kerry Le Benger, Charles K. Cooper, Valentin Parvu
Summary: This study aimed to evaluate the effectiveness and reliability of SARS-CoV-2 rapid antigen testing (RAT) in the real world. The results showed that RAT had a positivity rate of 25.3% in symptomatic or high-risk exposure patients, compared to only 3.4% in asymptomatic individuals. The reflex reverse transcriptase-polymerase chain reaction (RT-PCR) testing confirmed more than 90% of RAT positive results, with sensitivity values ranging from 77.2% to 85.6% and specificity values ranging from 97.9% to 99.9% under different assumptions.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Biochemistry & Molecular Biology
Maiko Mitake, Shiori Hirano, Tatsuya Kishino
Summary: Imprinted genes are expressed in a parent-of-origin-specific manner, discriminated by intragenic DNA polymorphisms. A novel quantitative method using ddPCR and LNA TaqMan probes was developed to analyze allelic expression of the imprinted gene Ube3a, revealing maternal allele predominance in neuronal tissues. Changes in Ube3a transcripts during postnatal brain development were also evaluated, demonstrating the reliability and simplicity of ddPCR in analyzing skewed or faint allelic expression.
Article
Agriculture, Dairy & Animal Science
HyeSoon Song, HyeonSu Kim, SiHyeon Kim, YongKuk Kwon, HyeRyoung Kim
Summary: The study developed a multiplex PCR assay for the simultaneous detection of ILTV, FWPV, REV-integrated FWPV, and REV, with a sensitivity of 1 x 54 copies/tube. The multiplex PCR showed 100% agreement with singleplex PCRs and sequencing when testing clinical samples, providing a simple, rapid, sensitive, specific, and cost-effective method for detecting the 4 viruses in clinical specimens.
Article
Public, Environmental & Occupational Health
M. Mentasti, S. David, K. Sands, S. Khan, L. Davies, L. Turner, M. Wootton
Summary: A multiplex real-time PCR assay was designed and validated for rapid and accurate detection and differentiation of mcr genes, demonstrating 100% sensitivity and specificity. The method is crucial for surveillance of mcr genes among Gram-negative bacteria.
JOURNAL OF HOSPITAL INFECTION
(2021)
Article
Infectious Diseases
Kewei Chen, Zhe Hu, Guangpu Yang, Wei Guo, Ting Qi, Diqiu Liu, Yaoxin Wang, Cheng Du, Xiaojun Wang
Summary: In this study, a duplex real-time PCR detection method was developed for the simultaneous detection and differentiation of T. equi and B. caballi infections in horses. The method exhibited good specificity, sensitivity, repeatability, and reproducibility, and was validated through comparison with other testing methods. This method is important for accurate diagnosis, pathogenic surveillance, and epidemiological investigation of T. equi and B. caballi infections.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Biochemical Research Methods
Veera Kainulainen, Sonja Elf, Petri Susi, Minna Maki, Anne Pitkaranta, Janne O. Koskinen, Riitta Korpela, Kevin E. Eboigbodin
JOURNAL OF VIROLOGICAL METHODS
(2019)
Article
Virology
Eero Hietanen, Petri Susi
Editorial Material
Virology
Petri Susi
Article
Virology
Teemu Smura, Soile Blomqvist, Pekka Kolehmainen, Isabelle Schuffenecker, Bruno Lina, Sindy Boettcher, Sabine Diedrich, Arthur Love, Mia Brytting, Elenor Hauzenberger, Susanne Dudman, Olga Ivanova, Alexander Lukasev, Thea Kolsen Fischer, Sofie Midgley, Petri Susi, Carita Savolainen-Kopra, Maija Lappalainen, Anne J. Jaaskelainen
JOURNAL OF CLINICAL VIROLOGY
(2020)
Article
Virology
F. Xavier Lopez-Labrador, Julianne R. Brown, Nicole Fischer, Heli Harvala, Sander Van Boheemen, Ondrej Cinek, Arzu Sayiner, Tina Vasehus Madsen, Eeva Auvinen, Verena Kufner, Michael Huber, Christophe Rodriguez, Marcel Jonges, Mario Hoenemann, Petri Susi, Hugo Sousa, Paul E. Klapper, Alba Perez-Cataluna, Marta Hernandez, Richard Molenkamp, Lia van der Hoek, Rob Schuurman, Natacha Couto, Karoline Leuzinger, Peter Simmonds, Martin Beer, Dirk Hoeper, Sergio Kamminga, Mariet C. W. Feltkamp, Jesus Rodriguez-Diaz, Els Keyaerts, Xiaohui Chen Nielsen, Elisabeth Puchhammer-Stockl, Aloys C. M. Kroes, Javier Buesa, Judy Breuer, Eric C. J. Claas, Jutte J. C. de Vries
Summary: Metagenomic high-throughput sequencing (mHTS) is a universal pathogen detection technique for determining DNA/RNA sequences, still in its early stages of clinical application. To support the development and standardization of mHTS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) has established the Network on Next-Generation Sequencing (ENNGS).
JOURNAL OF CLINICAL VIROLOGY
(2021)
Editorial Material
Virology
Petri Susi
Letter
Rheumatology
Olli Ruuskanen, Matti Waris
ARTHRITIS & RHEUMATOLOGY
(2022)
Article
Microbiology
Miia Laine, Jaakko Ahti, Ville Peltola, Piritta Peri, Antti J. Hakanen, Matti Waris
Summary: The study compared the diagnostic efficacy and tolerability of two types of nasopharyngeal swabs for COVID-19 testing. Results showed that the molded plastic swab was comparable to the standard flocked swab in terms of efficacy for respiratory virus detection and patient tolerance during sampling.
MICROBIOLOGY SPECTRUM
(2021)
Article
Biochemical Research Methods
Kari Kopra, Nazia Hassan, Emmiliisa Vuorinen, Salla Valtonen, Randa Mahran, Huda Habib, Pinja Jalkanen, Petri Susi, Vesa Hytonen, Minna Hankaniemi, Seppo Yla-Herttuala, Laura Kakkola, Markus Peurla, Harri Harma
Summary: Viruses play a major role in modern society, but current methods for counting viral particles are cumbersome and expensive. To address this issue, researchers have developed a simple and cost-effective time-resolved luminescence-based method that allows for rapid measurement of virus particle count.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Sport Sciences
Olli Ruuskanen, Raakel Luoto, Maarit Valtonen, Olli J. Heinonen, Matti Waris
Summary: Upper respiratory tract infections are common among elite athletes, but the impact of exercise on susceptibility to infections and the clinical implications of immune system changes need further virological studies.
Article
Virology
Eero Hietanen, Marika K. A. Koivu, Petri Susi
Summary: This study analyzed the genome sequences of Rigvir (R) and other echovirus 7 isolates, and found that Rigvir (R) is genetically distinct. Structural analysis revealed multiple mutations in the viral capsid proteins of Rigvir (R), including one in the region that interacts with decay-accelerating factor (DAF). Cell infectivity assays showed no significant difference in the oncolytic or oncotropic effect between Rigvir (R) and other isolates, and Rigvir (R) was also found to infect non-cancer cell lines. Therefore, this study concludes that Rigvir (R) is not an effective treatment for multiple types of cancers.
Article
Microbiology
Pekka Kolehmainen, Jemna Heroum, Pinja Jalkanen, Moona Huttunen, Laura Toivonen, Varpu Marjomaki, Matti Waris, Teemu Smura, Laura Kakkola, Sisko Tauriainen, Ville Peltola, Ilkka Julkunen
Summary: Seasonal coronaviruses cause respiratory infections in children, and the duration of antibody levels following the first infections is limited. This study found that seasonal coronavirus infections and reinfections are common in early childhood, and the antibody levels decline relatively rapidly.
MICROBIOLOGY SPECTRUM
(2022)
Article
Multidisciplinary Sciences
Eero Hietanen, Lav Tripathi, Eeva-Christine Brockmann, Pirjo Merilahti, Urpo Lamminmaki, Petri Susi
Summary: This study successfully isolated phage display antibodies against human parechoviruses, which may have broad applications in diagnostic antibody development.
SCIENTIFIC REPORTS
(2022)
Article
Virology
Niklas Lindblad, Timo Hanninen, Maarit Valtonen, Olli J. Heinonen, Matti Waris, Olli Ruuskanen
Summary: Influenza A outbreaks occurred in two professional hockey teams after they played against each other, resulting in 13 players and 2 staff members falling ill during the prevalent COVID-19 period. Seven players missed an important game due to influenza. Rapid diagnosis enabled effective control of the outbreaks through pharmaceutical and nonpharmaceutical measures.
Article
Sport Sciences
Raakel Luoto, Olli Ruuskanen, Johanna K. Ihalainen, Satu Pekkala, Jukka Hintikka, Noora Kanerva, Matti Waris, Olli J. Heinonen, Maarit Valtonen
Summary: The study found that Finnish elite cross-country skiers had lower concentrations of CRP but higher concentrations of IL-10, TNF-alpha, and IFN-gamma compared to moderately-exercising controls. There was no significant difference in IL-6 and GlycA levels between athletes and controls.
JOURNAL OF SCIENCE IN SPORT AND EXERCISE
(2023)