Article
Chemistry, Multidisciplinary
Ouyang Dan, Chuanzhe Wang, Chao Zhong, Juan Lin, Gang Xu, Guane Wang, Zian Lin
Summary: In this study, novel LDI-MS substrates were designed to enhance the sensitivity of mass signals and achieve high accuracy diagnosis of different saccharides and central precocious puberty (CPP).
Article
Microbiology
Anil K. Persad, Hanan A. Fahmy, Nicholas Anderson, Jing Cui, Zeynal Topalcengiz, Saharuetai Jeamsripong, Patrick M. Spanninger, Robert L. Buchanan, Kalmia E. Kniel, Michele T. Jay-Russell, Michelle D. Danyluk, Gireesh Rajashekara, Jeffrey T. LeJeune
Summary: This study demonstrates that MALDI-TOF is a viable alternative for the rapid identification and differentiation of Salmonella serovars.
Article
Biotechnology & Applied Microbiology
R. Gathercole, E. Tranfield, D. Xia, G. Perez-Cordon, G. Robinson, D. Timofte, F. Zendri, R. M. Chalmers
Summary: The study developed a method to purify Cryptosporidium spp. oocysts from clinical stool samples and evaluated them using mass spectrometry. The method produced distinct spectra specific to different Cryptosporidium species, demonstrating the utility of the purification method.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Chadaporn Chaiden, Janthima Jaresitthikunchai, Anusak Kerdsin, Nattakan Meekhanon, Sittiruk Roytrakul, Suphachai Nuanualsuwan
Summary: In this study, MALDI-TOF-MS analysis of extracted peptides was developed to improve the classification of S. suis serotypes. The method successfully differentiated highly pathogenic SS2 and SS14 from other serotypes, indicating its potential as an alternative approach for serotyping clinically pathogenic S. suis isolates.
Review
Microbiology
Xin-Fei Chen, Xin Hou, Meng Xiao, Li Zhang, Jing-Wei Cheng, Meng-Lan Zhou, Jing-Jing Huang, Jing-Jia Zhang, Ying-Chun Xu, Po-Ren Hsueh
Summary: MALDI-TOF MS has been used in clinical microbiology since 2010, offering advantages such as convenience, speed, accuracy, and low cost. It has shown promising results in rapid detection of highly virulent and drug-resistant pathogens, making it a valuable tool in clinical microbial identification.
Review
Biochemical Research Methods
Lanaia Itala Louzeiro Maciel, Ricardo Alves Bernardo, Rafael Oliveira Martins, Almir Custodio Batista Jr, Joao Victor Ataide Oliveira, Andrea Rodrigues Chaves, Boniek Gontijo Vaz
Summary: The imaging of biological tissues using mass spectrometry imaging (MSI) techniques allows for the visualization of the distribution of various metabolites, drugs, lipids, and glycans in complex samples. MSI methods like DESI-MSI and MALDI-MSI provide high sensitivity and the ability to evaluate/visualize multiple analytes in a single sample, overcoming limitations of classical microscopy techniques. This review discusses the application of DESI-MSI and MALDI-MSI in evaluating exogenous and endogenous molecules in biological samples, providing technical insights and recent research findings.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Infectious Diseases
Jichan Shi, Gexin Gao, Jing Pan, Lian-Peng Wu, Hongye Ning, Zhengxin Wu, Xinchun Ye, Xiangao Jiang
Summary: This study evaluated the clinical value of MALDI-TOF MS in detecting NTM by comparing the results with liquid culture and gene chip. The findings showed that MALDI-TOF MS had a high consistency with both methods and could be a rapid and effective method for identifying NTM strains and drug resistance.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Parasitology
Jurena Christiane Ebersbach, Marcello Otake Sato, Matheus Pereira de Araujo, Megumi Sato, Soeren L. Becker, Issa Sy
Summary: Schistosomiasis is a major tropical disease affecting millions of people worldwide. The current diagnostic methods have limitations, and this study developed and validated a species-specific database for adult Schistosoma identification. The study also evaluated the effects of different storage solutions on spectra profiles. The results showed that MALDI-TOF MS can reliably distinguish between S. mansoni and S. japonicum.
PARASITES & VECTORS
(2023)
Article
Immunology
John A. Fissel, Carrie K. Holdren-Serrell, Warda Memon, Sean X. Zhang
Summary: One of the challenges in fungal infection treatment is the long time required for species identification. MALDI-TOF MS has shown potential in rapidly identifying bacteria and yeasts, and this study explores its use for identifying filamentous molds. Most isolates in the study could be identified by MALDI-TOF MS within three days.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Plant Sciences
Ye Rin Jin, Myung Jin Oh, Heung Joo Yuk, Hyun Joo An, Dong Seon Kim
Summary: This study established a new mass spectrometry-based analysis procedure for red ginseng polysaccharides (RGPs), successfully qualitatively analyzing neutral and acidic RGPs, and identifying novel components. The new procedure shows great potential as a specific tool for searching and determining various polysaccharides in herbal medicines.
JOURNAL OF GINSENG RESEARCH
(2021)
Article
Plant Sciences
Yukun Liu, Xiaofei Nie, Jilong Wang, Zhenqi Zhao, Zhimei Wang, Fang Ju
Summary: Flavonoids in litchi seeds have broad-spectrum antiviral and antitumor activities, especially against breast and liver cancers. MALDI-MSI was used to detect and image the distribution of flavonoids in litchi seed tissue sections for the first time, revealing their heterogeneous distribution and potential for extraction.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Chi-Sheng Chen, Wen-Chi Chen, Sarah Y. Chang
Summary: A new mode of electromembrane extraction (EME) has been developed for detection via matrix-assisted laser desorption/ionization mass spectrometry (MALDI/MS). The method successfully extracted and analyzed posaconazole, and was applied to human serum samples.
Article
Food Science & Technology
Qi Wang, Xiaohui Li, Hongping Wang, Simeng Li, Chen Zhang, Xueying Chen, Jing Dong, Hua Shao, Jing Wang, Fen Jin
Summary: A matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) method was developed to obtain dynamic images of forchlorfenuron in oriental melons. The majority of forchlorfenuron was found in the exocarp and mesocarp regions of the melons and decreased within two days after application. The degradation rate obtained by MALDI-MSI was reliable and comparable to HPLC-MS/MS, indicating its practicability for pesticide degradation study.
Article
Chemistry, Applied
Suying Li, Tsz-Tsun Ng, Zhong-Ping Yao
Summary: This study developed a new method using MALDI-MS and PLS-R to quantitate triacylglycerols in blended oils, allowing simultaneous quantitation of multiple compositions with good limits of detection. The new method enables direct analysis of blended oils, rapid analysis of samples, and accurate quantitation of low-abundance oil compositions and blended oils with similar fatty acid contents, compared to conventional methods.
Article
Chemistry, Analytical
Frederic Fournelle, Nidia Lauzon, Ethan Yang, Pierre Chaurand
Summary: Traditionally, MALDI IMS requires the deposition of an organic matrix, which often generates high background noise in the low mass range. LDI offers selective ionization with low background in the low mass range. This article discusses the advantages and disadvantages of solvent free and wet deposition of metals as LDI matrices and demonstrates the power of metal-assisted LDI for analyzing low molecular weight molecules in tissue sections.
MICROCHEMICAL JOURNAL
(2023)
Review
Mycology
Teun Boekhout, Anthony S. Amend, Fouad El Baidouri, Toni Gabaldon, Jozsef Geml, Moritz Mittelbach, Vincent Robert, Chen Shuhui Tan, Benedetta Turchetti, Duong Vu, Qi-Ming Wang, Andrey Yurkov
Summary: Yeast, defined as unicellular fungi, are found in various fungal lineages and are not a taxonomic unit; it may take hundreds of years to fully document all yeast species. Many areas, including cold habitats and threatened environments, remain unsampled for yeasts. Studies have shown hybrid yeast species, prevalent yeast communities in natural habitats impacted by deforestation, and surprising global distributions of yeast species in soils and marine waters.
Article
Mycology
Husam Salah, Anna Kolecka, Anna Rozaliyani, Retno Wahyuningsih, Saad J. Taj-Aldeen, Teun Boekhout, Jos Houbraken
Summary: In this study, the authors developed an improved method for the identification of Aspergillus species using MALDI-TOF MS. By cultivating the fungi on a polycarbonate filter, they were able to obtain reliable mass spectra and achieve higher accuracy in species identification compared to the recommended liquid cultivation method. This method showed promise for the identification of other filamentous fungi as well.
Meeting Abstract
Infectious Diseases
Sadegh Khodavaisy, Mohammad Kord, Ahmad Elmimoghaddam, Seyed Jamal Hashemi, Sassan Reziae, Roshanak Daie Ghazvini, Mohammadreza Salehi, Alireza Abdollahi, Ali Ahmadi, Teun Boekhout
Article
Biochemistry & Molecular Biology
Gabriela Biliuta, Andra-Cristina Bostanaru-Iliescu, Mihai Mares, Carla Pavlov-Enescu, Valentin Nastasa, Olga Burduniuc, Sergiu Coseri
Summary: The aim of this study was to synthesize silver nanoparticles using cellulose derivatives and evaluate their antimicrobial potential. The results showed that cellulose acetate and hydroxypropyl cellulose produced more stable silver nanoparticles, which exhibited antimicrobial activity against various bacteria and fungi.
Article
Biochemistry & Molecular Biology
Cobus M. M. Visagie, Teun Boekhout, Bart Theelen, Jan Dijksterhuis, Neriman Yilmaz, Keith A. A. Seifert
Summary: A new species of the yeast genus Blastobotrys was discovered in house dust, with similar species detected globally in various substrates. The new species, Blastobotrys davincii, was classified based on morphological, phylogenetic, and physiological characters. The preferred name for the new species is still under debate, but Blastobotrys is argued to be more suitable than Trichomonascus. A nomenclatural review and accepted species list for Blastobotrys/Trichomonascus clade were also provided.
Article
Biochemistry & Molecular Biology
Andra-Cristina Enache, Petrisor Samoila, Corneliu Cojocaru, Adrian Bele, Andra-Cristina Bostanaru, Mihai Mares, Valeria Harabagiu
Summary: New amphiphilic chitosan membranes were developed for burns management and showed analgesic effect by delivering lidocaine as a local anesthetic. These membranes also demonstrated antibacterial activity against common pathogens in burns infections.
Article
Infectious Diseases
Husam Salah, Jos Houbraken, Teun Boekhout, Muna Almaslamani, Saad J. Taj-Aldeen
Summary: This study investigated the epidemiology and prevalence of non-Aspergillus and non-Fusarium filamentous fungi in human clinical samples in Qatar, and found that Mucorales was the major cause of invasive infections.
Article
Biotechnology & Applied Microbiology
Maria-Alexandra Martu, Ionut Luchian, Mihai Mares, Sorina Solomon, Oana Ciurcanu, Vlad Danila, Elena Rezus, Liliana Foia
Summary: Considering the increasing resistance of bacterial species to antibiotics and other antimicrobial agents, the development of alternative antimicrobial approaches is a major objective. Photodynamic disinfection therapy and laser irradiation are two methods that showed potential. In this in vitro study, the antimicrobial action of a diode laser and photodisinfection therapy on a periodontal pathogen, Aggregatibacter actinomycetemcomitans, was evaluated in the presence of autoimmune disease therapy drugs. Both treatments demonstrated a significant reduction in bacterial burden, indicating their potential as adjunctive measures for eradicating this oral pathogen in the context of immunomodulating therapy.
BIOENGINEERING-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Anita E. Nwaefuna, Teun Boekhout, Mar Garcia-Aloy, Urska Vrhovsek, Nerve Zhou
Summary: Yeasts associated with dung beetles, such as Meyerozyma and Pichia, are diverse and abundant. The study on dung beetles in Botswana revealed that their guts provide a rich niche for non-Saccharomyces yeast species. This research contributes to the understanding of the diversity of dung beetle-associated yeasts.
Article
Biochemistry & Molecular Biology
Camelia Mihaela Zara-Danceanu, Cristina Stavila, Anca Emanuela Minuti, Luminita Labusca, Valentin Nastasa, Dumitru-Daniel Herea, Razvan-Nicolae Malancus, Daniel Gherca, Sorin-Aurelian Pasca, Horia Chiriac, Mihai Mares, Nicoleta Lupu
Summary: Magnetic nanoparticles (MNPs) can be used as an efficient delivery system to reduce side effects and increase cell proliferation and chondrogenesis. Oil-in-water magneto nanoemulsions (MNEs) formulated with proprietary Fe3O4 MNPs carry ascorbic acid and dexamethasone, showing high colloidal stability, magnetic properties, and excellent biocompatibility in both in vitro and in vivo studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Mariana Grecu, Bogdan Minea, Liliana-Georgeta Foia, Andra-Cristina Bostanaru-Iliescu, Liviu Miron, Valentin Nastasa, Mihai Mares
Summary: Cyclodextrins are carrier molecules used to enhance the pharmacokinetic properties of therapeutic molecules. They form inclusion complexes with poorly soluble molecules and improve their solubility, bioavailability, stability, non-toxicity, and shelf life. β-CD derivatives, such as sulfobutylether-β-CD and hydroxypropyl-β-CD, are commonly used to overcome the toxicity issues associated with earlier inclusion complexes. This review highlights the diverse clinical applications of inclusion complexes in veterinary medicine and their contribution to improving drug efficacy, focusing on the biological activities of β-CD complexed molecules.
Article
Mycology
M. Groenewald, C. T. Hittinger, K. Bensch, D. A. Opulente, X. -X. Shen, Y. Li, C. Liu, A. L. LaBella, X. Zhou, S. Limtong, S. Jindamorakot, P. Goncalves, V. Robert, K. H. Wolfe, C. A. Rosa, T. Boekhout, N. Cadez, G. Peter, J. P. Sampaio, M. -A. Lachance, A. M. Yurkov, H. -M. Daniel, M. Takashima, K. Boundy-Mills, D. Libkind, K. Aoki, T. Sugita, A. Rokas
Summary: The subphylum Saccharomycotina is a diverse lineage within the fungal phylum Ascomycota, consisting of over 1,200 known species divided into 16 families, one order, and one class. It exhibits high genomic diversity and includes both opportunistic human pathogens and species important in biotechnological applications. However, the biotechnological potential of most yeast species remains unexplored. The current classification of Saccharomycotina as a single class and order underestimates its diversity, and an updated classification with seven classes and 12 orders is proposed based on genome content analysis and phylogenetic relationships.
STUDIES IN MYCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Dana A. Opulente, Quinn K. Langdon, Martin Jarzyna, Kelly V. Buh, Max A. B. Haase, Marizeth Groenewald, Chris Todd Hittinger
Summary: A novel budding yeast species was identified and analyzed through phylogenetic and genomic analyses, comparing its phenotypic data to other related species. The study revealed that the inability to utilize certain carbon and nitrogen sources was correlated with the absence of metabolic genes. Based on these findings, the new species was named Pichia galeolata sp. nov.
Article
Biotechnology & Applied Microbiology
Estifanos Hawaz, Mesfin Tafesse, Anteneh Tesfaye, Solomon Kiros, Dereje Beyene, Gessesse Kebede, Teun Boekhout, Marizeth Groenwald, Bart Theelen, Ayantu Degefe, Sisay Degu, Alene Admasu, Biru Hunde, Diriba Muleta
Summary: The purpose of this study was to optimize fermentation parameters to create favorable conditions for bioethanol production from sugar-rich substrates. The response surface method based on central composite design was used to determine the optimal conditions. The results confirmed the feasibility of achieving high ethanol yield using these conditions. The study demonstrated that the response surface method with central composite design is an effective approach for bioethanol production from molasses.
ANNALS OF MICROBIOLOGY
(2023)
Correction
Infectious Diseases
Husam Salah, Jos Houbraken, Teun Boekhout, Muna Almaslamani, Saad J. Taj-Aldeen