Article
Multidisciplinary Sciences
Samiratu Mahazu, Wakana Sato, Alafate Ayibieke, Isaac Prah, Takaya Hayashi, Toshihiko Suzuki, Shiroh Iwanaga, Anthony Ablordey, Ryoichi Saito
Summary: The emergence and dissemination of ESBL-producing bacteria, particularly Enterobacteriaceae, have posed serious challenges to healthcare in Ghana. This study investigated the antimicrobial susceptibility and genetic characteristics of 164 Escherichia coli strains isolated from infected patients in two hospitals. It was found that 62.2% of the cefotaxime-resistant isolates were ESBL-producers, with ST131 being the dominant group. The ESBL-producers carried different ESBL alleles, with bla(CTX-M-15) being the most predominant. This study provides the first report on the phylogeny of ST131 in Ghana.
SCIENTIFIC REPORTS
(2022)
Article
Infectious Diseases
Faustinos Tatenda Takawira, Johann Dd Pitout, Gaetan Thilliez, Tapfumanei Mashe, Ana Victoria Gutierrez, Robert A. Kingsley, Gisele Peirano, Jorge Matheu, Stanley M. Midzi, Lusubilo W. Mwamakamba, David L. Gally, Andrew Tarupiwa, Leckson Mukavhi, Marthie M. Ehlers, Sekesai Mtapuri-Zinyowera, Marleen M. Kock
Summary: This study investigated ESBL-producing extraintestinal pathogenic Escherichia coli associated with urinary tract infections in different regions of Zimbabwe from 2017 to 2019. The results showed ST131 and ST410 as the predominant resistant clones, with an increasing trend in ESBL-producing E. coli strains over time. The establishment and evolution of ESBL clones in Zimbabwe pose a significant public health threat, highlighting the importance of continuous monitoring and targeted control programs for specific STs, such as ST131 and ST410, to reduce the spread of ESBLs among ExPEC.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2021)
Article
Microbiology
Razib Mazumder, Arif Hussain, Ahmed Abdullah, Md Nazrul Islam, Md Tuhin Sadique, S. M. Muniruzzaman, Anika Tabassum, Farhana Halim, Nasrin Akter, Dilruba Ahmed, Dinesh Mondal
Summary: This study reveals a high diversity of E. coli lineages among ESBL-producing E. coli from Dhaka, with ongoing circulation of ST131 and other major non-ST131 high-risk clones that are strongly associated with cephalosporin resistance and virulence genes, highlighting the need for prospective monitoring of high-risk clones to prevent worsening of the AMR crises.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
Maria Giufre, Elena Mazzolini, Marina Cerquetti, Silvio Brusaferro
Summary: This study compared ESBL-producing Escherichia coli isolates from humans and food-producing animals, finding different types of ESBLs in different sources. The results suggest the potential exchange of ESBL genes from animals to humans, emphasizing the need for strict monitoring based on a 'One Health' approach.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Article
Multidisciplinary Sciences
Maria Muddassir, Almas Raza, Sadaf Munir, Ahmad Basirat, Muddassir Ahmed, Mazia Shahid Butt, Omair Arshad Dar, Syed Shoaib Ahmed, Saba Shamim, Syed Zeeshan Haider Naqvi
Summary: This study investigated the antibiogram and drug resistance genes of carbapenem-resistant Pseudomonas aeruginosa, as well as the potency of Silver Nanoparticles against multidrug-resistant strains. The results showed that the conjugated effect of silver nanoparticles with antibiotics effectively inhibited multidrug-resistant Pseudomonas aeruginosa.
SCIENTIFIC REPORTS
(2022)
Article
Infectious Diseases
Jan Koren, Michal Andrezal, Elham Ozaee, Hana Drahovska, Martin Wawruch, Adriana Liptakova, Tibor Maliar
Summary: The study analyzed E. coli strains from two healthcare facilities in University Hospital Bratislava and found the emergence of ST131 ESBL-producing E. coli, which poses major challenges worldwide, with the majority originating from the first hospital. Additionally, investigation on ST131 and clonal relatedness indicated a possible epidemiological association.
Article
Infectious Diseases
Toyotaka Sato, Shin-ichi Yokota, Tooru Tachibana, Satoshi Tamai, Shigeki Maetani, Yutaka Tamura, Motohiro Horiuchi
Summary: The study found that companion animals can carry fluoroquinolone-resistant Escherichia coli, which may be associated with human clinical infections and can potentially be transmitted to their owners.
Article
Microbiology
Amin Khoshbayan, Rezvan Golmoradi Zadeh, Majid Taati Moghadam, Shiva Mirkalantari, Atieh Darbandi
Summary: This study found the importance and high prevalence of E. coli O25b/ST131 among UTI isolates, with the ability to rapidly spread and disseminate antibiotic resistance genes.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2022)
Article
Infectious Diseases
Jiayu Shao, Hangdong Dai, Liwei Xu, Shuilong Zhu, Jufang Zhu, Hangyu Fu, Minxia Ge, Xianhong He
Summary: The purpose of this study was to investigate the genomic characteristics and antimicrobial resistance mechanisms of ESBL-producing E. coli isolates recovered from a district hospital in China. The results showed that these isolates exhibited resistance to multiple antibiotics, with the majority belonging to the ST131 serotype and having different virulence genes and sequence types. Therefore, continuous surveillance and control of the transmission of these multi-drug resistant bacteria in clinical and community settings are crucial.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Infectious Diseases
Antonio Mandujano, Diana Veronica Cortes-Espinosa, Jose Vasquez-Villanueva, Paulina Guel, Gildardo Rivera, Karina Juarez-Rendon, Wendy Lizeth Cruz-Pulido, Guadalupe Aguilera-Arreola, Abraham Guerrero, Virgilio Bocanegra-Garcia, Ana Veronica Martinez-Vazquez
Summary: This study investigated the antimicrobial resistance profile of ESBL-producing E. coli among food-producing animals in Tamaulipas, Mexico. A total of 200 fecal samples were collected and 5.0% of the strains were confirmed as ESBL producers. High percentage of antimicrobial resistance was observed against gentamicin, tetracycline, and streptomycin. The study suggests that food-producing animals may serve as reservoirs and contribute to the spread of ESBL-producing bacteria.
Article
Microbiology
Mayo Yasugi, Shingo Hatoya, Daisuke Motooka, Daisuke Kondo, Hideo Akiyoshi, Masayuki Horie, Shota Nakamura, Terumasa Shimada
Summary: The presence of mcr plasmid-mediated colistin-resistant ESBL-producing Enterobacterales in companion dogs and cats in Japan was identified. The isolates from a dog and a cat carried different mcr genes and bla genes on plasmids. This suggests that companion dogs and cats may serve as important reservoirs for cross-species transmission of the mcr gene.
VETERINARY MICROBIOLOGY
(2023)
Article
Microbiology
Kinga Toth, Akos Toth, Katalin Kamotsay, Viktoria Nemeth, Dora Szabo
Summary: This study reveals that ST131 clone, particularly C2/H30Rx and C1-M27 subclades, is the dominant clone of ESBL-producing E. coli collected in a hospital in Hungary.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2022)
Article
Engineering, Environmental
Aradhana Basu, Ananyo Jyoti Misra, Meerambika Behera, Susanta Kumar Behera, Ashish Kumar Nayak, Nabin Kumar Dhal, Amrita Mishra, Bijoy Kumar Satpathy, Cecilia Stalsby Lundborg, Suraj K. Tripathy
Summary: The study demonstrates successful photocatalytic disinfection of MDR Escherichia coli using Alumina/ZnO heterostructures. The proposed technique shows efficacy in disinfecting various bacteria and exhibits biocompatibility towards animal models.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Infectious Diseases
Kazuma Sakaeda, Takuya Sadahira, Yuki Maruyama, Takehiro Iwata, Masami Watanabe, Koichiro Wada, Motoo Araki
Summary: We conducted a molecular biological analysis of ESBL-producing E. coli strains and their sensitivity to FMOX. Most of the strains belonged to the ST-131 sequence type and had CTX-M genotypes. The isolated strains were sensitive to FMOX in drug susceptibility tests. FMOX showed efficacy for perioperative prophylactic administration in urologic surgery and for therapeutic administration for urinary tract infections.
Article
Infectious Diseases
Tyler J. Stone, Abdullah Kilic, John C. Williamson, Elizabeth L. Palavecino
Summary: The in vitro activity of omadacycline and comparator antibiotics against clinical ESBL-producing and non-ESBL-producing E. coli and K. pneumoniae urinary isolates was evaluated. Results showed that 54.9% of all ESBL-producing isolates and 74.5% of ESBL-producing E. coli isolates were susceptible to omadacycline. Overall, omadacycline and nitrofurantoin were the most active agents for treating UTI caused by ESBL-producing E. coli.
Article
Infectious Diseases
Francesc Escrihuela-Vidal, Achim J. Kaasch, Maja Von Cube, Siegbert Rieg, Winfried V. Kern, Harald Seifert, Kyoung-Ho Song, Chun-Hsing Liao, Robert Tilley, Hannah Gott, Matt Scarborough, Claire Gordon, Martin J. Llewelyn, Richard Kuehl, Laura Morata, Alex Soriano, Jonathan Edgeworth, Enrique Ruiz De Gopegui, Emmanuel Nsutebu, Jose Miguel Cisneros, Vance G. Fowler, Guy Thwaites, Joaquin Lopez-Contreras, Gavin Barlow, Hugo Guillermo Ternavasio-De La Vega, Jesus Rodriguez-Bano, Luis Eduardo Lopez-Cortes
Summary: This study analysed the adherence and impact of quality-of-care indicators (QCIs) in the management of Staphylococcus aureus bloodstream infection. The results showed that adherence to QCIs was associated with reduced 90-day mortality.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Review
Oncology
Elena Carrara, Elisa Razzaboni, Anna Maria Azzini, Maria Elena De Rui, Mariana Nunes Pinho Guedes, Anna Gorska, Maddalena Giannella, Linda Bussini, Michele Bartoletti, Federica Arbizzani, Zaira R. Palacios-Baena, Giulia Caponcello, Natalia Maldonado, Jesus Rodriguez-Bano, Carlo Visco, Mauro Krampera, Evelina Tacconelli
Summary: The main aim of this systematic review is to quantify the risk and identify predictors of clinical progression of SARS-CoV-2 in hematological patients compared to different control populations. The study found that hematological patients had a higher risk of COVID-19-related mortality, hospitalization, intensive-care admission, and mechanical ventilation compared to controls. Older age, male sex, and hypertension were significantly related to worse clinical outcomes in hematological patients with COVID-19.
HEMATOLOGICAL ONCOLOGY
(2023)
Article
Infectious Diseases
Alaa Atamna, Ili Margalit, Gida Ayada, Tanya Babich, Pontus Naucler, John Karlsson Valik, Christian G. Giske, Natividad Benito, Ruben Cardona, Alba Rivera, Celine Pulcini, Manal Abdel Fattah, Justine Haquin, Alasdair Macgowan, Bibiana Chazan, Anna Yanovskay, Ronen Ben Ami, Michal Landes, Lior Nesher, Adi Zaidman-Shimshovitz, Kate McCarthy, David L. Paterson, Evelina Tacconelli, Michael Buhl, Susanna Mauer, Jesus Rodriguez-Bano, Marina de Cueto, Antonio Oliver, Enrique Ruiz de Gopegui, Angela Cano, Isabel Machuca, Monica Gozalo-Marguello, Luis Martinez-Martinez, Eva M. Gonzalez-Barbera, Iris Gomez Alfaro, Miguel Salavert, Bojana Beovic, Andreja Saje, Manica Mueller-Premru, Leonardo Pagani, Virginie Vitrat, Diamantis Kofteridis, Maria Zacharioudaki, Sofia Maraki, Yulia Weissman, Mical Paul, Yaakov Dickstei, Dafna Yahav
Summary: This study aimed to evaluate outcomes of P. aeruginosa bacteremia among older adults (> 80 years) and found that disease severity, place of acquisition, and background conditions were associated with mortality, while antimicrobial regimen was not. Therefore, preventive efforts and early diagnosis might be beneficial for improving outcomes in older adults with P. aeruginosa bacteremia.
Article
Medicine, General & Internal
Adi Turjeman, Elodie von Dach, Jose Molina, Erica Franceschini, Fidi Koppel, Dana Yelin, Yael Dishon-Benattar, Cristina Mussini, Jesus Rodriguez-Bano, Jose Miguel Cisneros, Angela Huttner, Mical Paul, Leonard Leibovici, Dafna Yahav
Summary: This study aims to compare the effect of short and long-term treatment in Gram-negative bacteremia on all-cause mortality in pre-specified subgroups. The results show that in patients with stable hemodynamics and normal temperature for 48 hours prior to discontinuation, seven days of antibiotic therapy for enterobacterales bacteremia result in similar outcomes as 14 days.
Article
Surgery
Elena Perez-Nadales, Mario Fernandez-Ruiz, Alejandra M. Natera, Belen Gutierrez-Gutierrez, Alessandra Mularoni, Giovanna Russelli, Ligia Camera Pierrotti, Maristela Pinheiro Freire, Marco Falcone, Giusy Tiseo, Mario Tumbarello, Francesca Raffaelli, Edson Abdala, Marta Bodro, Elena Gervasi, Maria Carmen Farinas, Elena M. Seminari, Juan Jose Caston, Juan Antonio Marin-Sanz, Victor Galvez-Soto, Meenakshi M. Rana, Belen Loeches, Pilar Martin-Davila, Alvaro Pascual, Jesus Rodriguez-Bano, Jose Maria Aguado, Luis Martinez-Martinez, Julian Torre-Cisneros, REIPI INCREMENT-SOT Study Grp
Summary: This study compared the efficacy of ceftazidime-avibactam (CAZ-AVI) and the best available therapy (BAT) in treating solid organ transplant recipients with bloodstream infection caused by carbapenemase-producing Klebsiella pneumoniae. The results showed that patients treated with CAZ-AVI had higher clinical success rates at 14-day and 30-day and lower 30-day mortality compared to those receiving BAT.
AMERICAN JOURNAL OF TRANSPLANTATION
(2023)
Article
Microbiology
S. Diaz-Diaz, E. Recacha, Marina R. Pulido, Maria Romero-Munoz, B. de Gregorio-Iaria, F. Docobo-Perez, A. Pascual, J. M. Rodriguez-Martinez
Summary: The suppression of the SOS response and Dam methylation system enhances the in vitro activity of quinolones. Dual targeting of the recA (SOS response) and Dam methylation system genes increases the sensitivity of Escherichia coli to quinolones, even in a resistant strain model. This genetic and microbiological approach demonstrates the potential for preventing the evolution of resistance by suppressing both systems in quinolone-resistant strains.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Maddalena Giannella, Manuel Huth, Elda Righi, Jan Hasenauer, Lorenzo Marconi, Angelina Konnova, Akshita Gupta, An Hotterbeekx, Matilda Berkell, Zaira R. Palacios-Baena, Maria Cristina Morelli, Mariarosa Tame, Marco Busutti, Luciano Potena, Elena Salvaterra, Giuseppe Feltrin, Gino Gerosa, Lucrezia Furian, Patrizia Burra, Salvatore Piano, Umberto Cillo, Mara Cananzi, Monica Loy, Gianluigi Zaza, Francesco Onorati, Amedeo Carraro, Fiorella Gastaldon, Maurizio Nordio, Samir Kumar-Singh, Jesus Rodriguez Bano, Tiziana Lazzarotto, Pierluigi Viale, Evelina Tacconelli
Summary: This study aimed to assess the predictors of negative antibody response in solid organ transplant recipients after the first booster of SARS-CoV-2 vaccination. Machine learning models and clinical data were used to identify age, type of transplant, time between transplant and vaccination, immunosuppressive drugs, type of vaccine, and graft function as significant predictors of negative antibody response. This study is of significant importance for improving vaccination strategies in solid organ transplant recipients.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Infectious Diseases
Jesus Sojo-Dorado, Inmaculada Lopez-Hernandez, Alicia Hernandez-Torres, Pilar Retamar-Gentil, Esperanza Merino de Lucas, Laura Escola-Verge, Elena Bereciartua, Elisa Garcia-Vazquez, Vicente Pintado, Lucia Boix-Palop, Clara Natera-Kindelan, Luisa Sorli, Nuria Borrell, Concha Amador-Prous, Evelyn Shaw, Alfredo Jover-Saenz, Jose Molina, Rosa M. Martinez-Alvarez, Carlos J. Duenas, Jorge Calvo-Montes, Maria Lecuona, Virginia Pomar, Irene Borreguero, Virginia Palomo-Jimenez, Fernando Docobo-Perez, Alvaro Pascual, Jesus Rodriguez-Bano
Summary: This study compared the effectiveness and safety of fosfomycin trometamol and other oral drugs as step-down therapy for multidrug-resistant Escherichia coli (MDR-Ec) induced bacteremic urinary tract infections (BUTI). The results showed no significant difference in clinical and microbiological cure between fosfomycin trometamol and other drugs, but the relapse rate was higher in the fosfomycin trometamol group. Therefore, fosfomycin trometamol may be a reasonable option for step-down therapy in BUTI, but the relapse rate needs further evaluation.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Medicine, General & Internal
Laure F. Pittet, Nicole L. Messina, Francesca Orsini, Cecilia L. Moore, Veronica Abruzzo, Simone Barry, Rhian Bonnici, Marc Bonten, John Campbell, Julio Croda, Margareth Dalcolmo, Kaya Gardiner, Grace Gell, Susie Germano, Adriano Gomes-Silva, Casey Goodall, Amanda Gwee, Tenaya Jamieson, Bruno Jardim, Tobias R. Kollmann, Marcus V. G. Lacerda, Katherine J. Lee, Michaela Lucas, David J. Lynn, Laurens Manning, Helen S. Marshall, Ellie McDonald, Craig F. Munns, Suellen Nicholson, Abby O'Connell, Roberto D. de Oliveira, Susan Perlen, Kirsten P. Perrett, Cristina Prat-Aymerich, Peter C. Richmond, Jesus Rodriguez-Bano, Glauce dos Santos, Patricia V. da Silva, Jia Wei Teo, Paola Villanueva, Adilia Warris, Nicholas J. Wood, Andrew Davidson, Nigel Curtis
Summary: In this international trial, healthcare workers were randomly assigned to receive either the BCG-Denmark vaccine or a saline placebo. After 6 months, it was found that the BCG vaccine did not reduce the risk of symptomatic or severe Covid-19 compared to placebo.
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Article
Multidisciplinary Sciences
Carmen Garrigos, Clara Maria Rosso-Fernandez, Irene Borreguero, Patricia Rodriguez, Raquel Garcia-Albea, Jose Maria Bravo-Ferrer, Jesus Rodriguez-Bano, Maria Dolores del Toro
Summary: This study aims to investigate the optimal duration of antibiotic treatment in infection associated with osteosynthesis material (IOM) after long bone fractures. The hypothesis is that a shorter duration of antibiotic treatment can reduce antibiotic exposure, control antimicrobial resistance, and lower adverse events and costs, while still maintaining a high cure rate and functional recovery.
Article
Microbiology
T. Blanco-Martin, J. Guzman-Puche, C. Riazzo, M. Gasca-Santiyan, M. Hernandez-Garcia, R. Canton, J. Torre-Cisneros, C. Herrera, L. Martinez-Martinez
Summary: IMI carbapenemases confer an unusual resistance pattern, making them difficult to detect. Commercial molecular methods usually do not include bla(IMI) genes, contributing to the hidden dissemination of bacteria producing these enzymes. We report a plasmid-encoded IMI-6 carbapenemase in an Enterobacter ludwigii isolate, providing insights into its genetic characteristics.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Beatriz Fernandez-Rubio, Laura Herrera-Hidalgo, Luis E. Lopez-Cortes, Rafael Luque-Marquez, Aristides De Alarcon, Sonia Luque-Pardos, Aurora Fernandez-Polo, Jose Maria Gutierrez-Urbon, Jesus Rodriguez-Bano, Maria Victoria Gil-Navarro, Alicia Gutierrez-Valencia
Summary: This study evaluated the physical and chemical stability of temocillin at different temperatures and stored in different containers at the recommended dose for OPAT. It was found that temocillin is stable under OPAT conditions and can be an appropriate treatment option for patients. A novel LC-MS/MS method was also developed for the quantification of temocillin.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Irene Sanchez-Leon, Teresa Garcia-Martinez, Seydina M. Diene, Elena Perez-Nadales, Luis Martinez-Martinez, Jean-Marc Rolain
Summary: This study aimed to investigate heteroresistance in nine clinical isolates of Klebsiella pneumoniae producing OXA-48 and describe genomic changes in vitro. It was found that the heteroresistance in K. pneumoniae isolates was mainly attributed to insertions in the mgrB gene and point mutations in colistin resistance proteins. These findings contribute to the understanding of colistin resistance mechanisms in OXA-48-producing K. pneumoniae mutants.
Article
Veterinary Sciences
Alice Ferreira, Daniela Silva, Carina Almeida, Maria Elisa Rodrigues, Sonia Silva, Joana Castro, Dalila Mil-Homens, Isidro Garcia-Menino, Azucena Mora, Mariana Henriques, Ana Oliveira
Summary: This study evaluated the efficacy of phage vB_EcoM_FJ1 (FJ1) in reducing the load of pathogenic Escherichia coli (ETEC) and found that encapsulated FJ1 in microparticles significantly reduced the quantity of ETEC. Bacteriophage-insensitive mutants (BIMs) displayed weaker adaptability in the pig model.
VETERINARY RESEARCH
(2023)
Article
Microbiology
Irene Sanchez-Leon, Elena Perez-Nadales, Juan Antonio Marin-Sanz, Teresa Garcia-Martinez, Luis Martinez-Martinez
Summary: This study investigated heteroresistance in 10 wild-type Klebsiella pneumoniae isolates of clinical origin. The results showed the importance of persistent variants and stable mutants in heteroresistance, and described the genomic changes observed in mutants obtained in population analysis profiling (PAP) assays.
MICROBIOLOGY SPECTRUM
(2023)