Editorial Material
Chemistry, Medicinal
Tommaso Felicetti, Serena Massari
Summary: The high number of existing protein-protein interactions presents an unprecedented opportunity for identifying novel drug targets in medicinal chemistry. PPIs serve as new targets for the development of small molecule drugs.
FUTURE MEDICINAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Elisabeth Mercier, Patrick M. D'Aoust, Ocean Thakali, Nada Hegazy, Jian-Jun Jia, Zhihao Zhang, Walaa Eid, Julio Plaza-Diaz, Md Pervez Kabir, Wanting Fang, Aaron Cowan, Sean E. Stephenson, Lakshmi Pisharody, Alex E. MacKenzie, Tyson E. Graber, Shen Wan, Robert Delatolla
Summary: Global clinical surveillance is crucial for monitoring and reporting influenza strains, while wastewater surveillance (WWS) can serve as a less resource-intensive alternative. Our study shows that influenza virus targets are stable in wastewater and can be used to forecast flu outbreaks with a 17-day lead time, providing real-time viral subtyping at the municipal and neighborhood levels using minimal resources and infrastructure.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Kelsey M. Haas, Michael J. Mcgregor, Mehdi Bouhaddou, Benjamin J. Polacco, Eun-Young Kim, Thong T. Nguyen, Billy W. Newton, Matthew Urbanowski, Heejin Kim, Michael A. P. Williams, Veronica V. Rezelj, Alexandra Hardy, Andrea Fossati, Erica J. Stevenson, Ellie Sukerman, Tiffany Kim, Sudhir Penugonda, Elena Moreno, Hannes Braberg, Yuan Zhou, Giorgi Metreveli, Bhavya Harjai, Tia A. Tummino, James E. Melnyk, Margaret Soucheray, Jyoti Batra, Lars Pache, Laura Martin-Sancho, Jared Carlson-Stevermer, Alexander S. Jureka, Christopher F. Basler, Kevan M. Shokat, Brian K. Shoichet, Leah P. Shriver, Jeffrey R. Johnson, Megan L. Shaw, Sumit K. Chanda, Dan M. Roden, Tonia C. Carter, Leah C. Kottyan, Rex L. Chisholm, Jennifer A. Pacheco, Maureen E. Smith, Steven J. Schrodi, Randy A. Albrecht, Marco Vignuzzi, Lorena Zuliani-Alvarez, Danielle L. Swaney, Manon Eckhardt, Steven M. Wolinsky, Kris M. White, Judd F. Hultquist, Robyn M. Kaake, Adolfo Garcia-Sastre, Nevan J. Krogan
Summary: This study provides a comprehensive network model of IAV infection in human cells, identifying functional host targets for pan-viral HDT. Key host proteins essential for IAV infection are revealed, and gene variants associated with severe influenza are discovered. Moreover, compounds targeting identified host factors suppress IAV replication and exhibit broad antiviral activity across influenza and coronavirus families.
NATURE COMMUNICATIONS
(2023)
Article
Medicine, General & Internal
Marina Rubio-Garrido, Jose Avendano-Ortiz, Adolphe Ndarabu, Carolina Rubio, Gabriel Reina, Eduardo Lopez-Collazo, Africa Holguin
Summary: Programs to prevent mother-to-child HIV transmission do not reduce the number of infants exposed during pregnancy and breastfeeding, and HIV-exposed but uninfected children face higher risk of morbidity and mortality. The study of immune biomarkers in HIV and the use of DBS could help predict disease progression and monitor immune responses effectively.
FRONTIERS IN MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Barbara Szutkowska, Klaudia Wieczorek, Ryszard Kierzek, Pawel Zmora, Jake M. Peterson, Walter N. Moss, David H. Mathews, Elzbieta Kierzek
Summary: This study proposes a secondary structure for segment 8 vRNA of A/California/04/2009 (H1N1) for the first time and highlights the location of conserved structural motifs within IAV strains. The research has implications for understanding the functionality of viral RNA structures and developing inhibition methods against influenza A virus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Siwen Liu, Bobo Wing-Yee Mok, Shaofeng Deng, Honglian Liu, Pui Wang, Wenjun Song, Pin Chen, Xiaofeng Huang, Min Zheng, Siu-Ying Lau, Conor J. Cremin, Chun-Yee Tam, Baiying Li, Liwen Jiang, Yixin Chen, Kwok-Yung Yuen, Honglin Chen
Summary: Host adaptive mutations in the influenza A virus PB2 protein are critical for human infection, with avian PB2 virus inducing higher autophagic flux in infected cells compared to mammalian PB2 virus. The formation of vRNP aggregates is essential for host restriction of avian PB2 IAV, with p62 and its interaction with vRNPs playing a key role in this process. This study reveals a novel strategy for host restriction of avian PB2 IAV through selective autophagic sequestration during late-stage virus replication.
Article
Virology
Kareem E. Hassan, Ann Kathrin Ahrens, Ahmed Ali, Magdy F. El-Kady, Hafez M. Hafez, Thomas C. Mettenleiter, Martin Beer, Timm Harder
Summary: This article introduces a real-time RT-PCR method for comprehensive subtyping of hemagglutinin and neuraminidase subtypes of AIV by introducing a total of 24 arrayed RT-qPCR reactions. The technique not only allows for diagnosis of AIV infections but also includes detection of other viruses. The method demonstrates good inclusivity and exclusivity in validation runs and is suitable for influenza A virus in avian hosts.
Article
Medicine, Research & Experimental
Yuchong Wang, Xujun Zhang, Kefan Bi, Hongyan Diao
Summary: MicroRNAs play important roles in regulating virus replication and host antiviral response during H1N1 infection, with some defending against the virus and others promoting viral replication. They are involved in host-viral interactions and have versatile functions in this process.
Article
Biology
Jennifer E. Jones, Valerie Le Sage, Gabriella H. Padovani, Michael Calderon, Erik S. Wright, Seema S. Lakdawala
Summary: Influenza viruses evolve rapidly during infection through mutations or reassortment, potentially leading to strains with increased resistance or infectivity. Understanding how genome segments interact during reassortment can help predict future events and emergence of new pandemic strains. Research in this area could provide valuable insights for risk assessment and pandemic preparedness.
Article
Multidisciplinary Sciences
Brian Reinhardt, Robert Taylor, Colin Dawkins, Taylor Banks, Nora Watson, Appavu Sundaram, Daniel Ewing, Janine Ruth Danko
Summary: Dried blood spot (DBS) sampling is desirable for assessing the immune status of individuals following infection or vaccination. This study compared DBS to traditional blood sampling in detecting antibodies against different pathogens. The results showed that DBS sampling was highly sensitive, specific, and correlated with traditional sampling for antibodies against measles, tetanus, and varicella zoster, but not hepatitis A and influenza.
Review
Biochemistry & Molecular Biology
Zihan Zhu, Ervin Fodor, Jeremy R. Keown
Summary: Influenza virus replication relies on the RNA-dependent RNA polymerase and viral ribonucleoprotein complex. Recent structural data have provided insights into the molecular mechanisms of influenza virus genome replication, particularly in terms of the encapsidation of nascent RNA, de novo assembly of ribonucleoprotein complexes, and the regulation of replication initiation through interactions with host and viral cues.
TRENDS IN MICROBIOLOGY
(2023)
Review
Virology
Xiuli Li, Min Gu, Qinmei Zheng, Ruyi Gao, Xiufan Liu
Summary: Influenza A virus (IAV) possesses a genome consisting of eight single-stranded, negative-sense RNA segments that encode 17 proteins. The selective genome packaging mechanism during assembly relies on RNA-RNA or protein-RNA interactions. Packaging signals are specific structures on viral RNA that play important roles in determining virus replication, genome incorporation, and genetic reassortment.
Article
Microbiology
Fernando Villalon-Letelier, Andrew G. Brooks, Sarah L. Londrigan, Patrick C. Reading
Summary: MARCH8 restricts influenza A virus at a late stage in replication by reducing incorporation of envelope glycoproteins into virions, demonstrating a unique mechanism of antiviral activity. The N-terminal cytoplasmic domain of MARCH8 is essential for this anti-IAV activity, distinguishing it from MARCH1.
Article
Biochemistry & Molecular Biology
Jie Min, Ying Cao, Haizhou Liu, Di Liu, Wenjun Liu, Jing Li
Summary: This study profiles the expression of circRNA, microRNA, and mRNA in human lung carcinoma cells in response to avian influenza virus (AIV) or human influenza A virus (IAV) infection. It identifies specific circRNAs that are up-regulated by AIV infection and demonstrates their role in regulating viral replication and proliferation by affecting the Endocytosis pathway and the Cell cycle process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Wei Li, Hongnuan Wang, Shijun J. Zheng
Summary: Influenza virus and coronavirus are important respiratory viruses that cause serious diseases in humans and animals. Understanding the relationship between viral infection and host innate immunity is crucial for effective control strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pathology
Carmichael Ong, Warren Tai, Aartik Sarma, Steven M. Opal, Andrew W. Artenstein, Anubhav Tripathi
JOURNAL OF MOLECULAR DIAGNOSTICS
(2012)
Article
Pathology
Kenneth Morabito, Rami Kantor, Warren Tai, Leeann Schreier, Anubhav Tripathi
JOURNAL OF MOLECULAR DIAGNOSTICS
(2013)
Article
Cardiac & Cardiovascular Systems
Tarsha Ward, Warren Tai, Sarah Morton, Francis Impens, Petra Van Damme, Delphi Van Haver, Evy Timmerman, Gabriela Venturini, Kehan Zhang, Min Young Jang, Jon A. L. Willcox, Alireza Haghighi, Bruce D. Gelb, Wendy K. Chung, Elizabeth Goldmuntz, George A. Porter, Richard P. Lifton, Martina Brueckner, H. Joseph Yost, Benoit G. Bruneau, Joshua Gorham, Yuri Kim, Alexandre Pereira, Jason Homsy, Craig C. Benson, Steven R. DePalma, Sylvia Varland, Christopher S. Chen, Thomas Arnesen, Kris Gevaert, Christine Seidman, J. G. Seidman
Summary: The study explores the effects of NAA15 haploinsufficiency on cardiac development by introducing NAA15 variants into human induced pluripotent stem cells. It demonstrates that NAA15 haploinsufficiency results in decreased N-terminal acetylation levels and altered steady-state protein levels, impacting various proteins involved in cardiac development.
CIRCULATION RESEARCH
(2021)
Editorial Material
Cardiac & Cardiovascular Systems
Warren Tai, Gary C. Doolittle, Zubair Shah, James B. Atkinson, Elaine Russell, Randall E. Genton, Javid J. Moslehi, Charles B. Porter
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2022)