Rapid and Simultaneous Detection of blaKPC and blaNDM by Use of Multiplex Real-Time PCR

The increasing incidence of carbapenem nonsusceptibility among clinically important species is of global concern. Identification of the molecular mechanisms underlying carbapenem nonsusceptibility is critical for epidemiological investigations. In this report, we describe a real-time PCR-based assay capable of simultaneously detecting bla(KPC) and bla(NDM), two of the most important carbapenemases, directly from culture in less than 90 min. The assay was validated with bla(KPC)- and bla(NDM)-carrying clinical isolates and demonstrated 100% concordance with the Carba NP test.