Article
Chemistry, Multidisciplinary
Amanda Victorious, Zijie Zhang, Dingran Chang, Roderick Maclachlan, Richa Pandey, Jianrun Xia, Jimmy Gu, Todd Hoare, Leyla Soleymani, Yingfu Li
Summary: On-farm testing of infectious diseases in farm animals is crucial for prevention and timely intervention. This study introduces a rapid detection test for porcine epidemic diarrhea viruses (PEDv) using a dual-electrode electrochemical chip and a barcode-releasing electroactive aptamer. The test showed high sensitivity and specificity, with results available within one hour.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biotechnology & Applied Microbiology
Zongyang Huang, Wandi Zhang, Langju Su, Guangmiao Ma, Jinyue Guo, Yunxiang Zhao, Weiqian Huang, Weichao Zhang, Saeed El-Ashram, Zhili Li
Summary: In this study, nine L. reuteri strains were isolated from swine feces, and LRC8 showed a higher inhibition rate against PEDV compared to other strains. The LRC8 strain demonstrated good growth ability, acid and bile salt tolerance, and strong adhesion to IPEC-J2 cells. Moreover, the metabolites of LRC8 (LRM) showed anti-inflammatory and antiviral activities against PEDV. The LRC8 strain effectively relieved clinical symptoms and intestinal damage in piglets infected with PEDV.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Microbiology
Yingshuo Sun, Ting Gong, Dongdong Wu, Yongzhi Feng, Qi Gao, Jiabao Xing, Xiaoyu Zheng, Zebu Song, Xing Liu, Xiongnan Chen, Yankuo Sun, Guihong Zhang, Lang Gong
Summary: In this study, two strains of PEDV were isolated from diarrheal samples collected from two new PED outbreak farms in 2022. The strains were analyzed for their gene sequences and pathogenicity. The results showed that both strains belong to the G2c subtype and can cause severe diarrhea and intestinal tissue lesions in infected piglets. It was also found that PEDV can be detected in various organs, with the highest levels in the intestinal tissues.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Yufang Ge, Feiyang Jiang, Sibei Wang, Heqiong Wu, Yuan Liu, Bin Wang, Wei Hou, Xiuju Yu, Haidong Wang
Summary: During the long-term co-evolution, vaccines may emerge with incomplete protective immunity due to mutations or deletions at specific antigenic sites. Researching and developing new vaccines with cross-protection is important to evaluate current vaccines' efficacy. The prevalence and evolutionary characteristics of PEDV in certain areas of China were examined to contribute to the development of new PEDV vaccines.
Article
Virology
Christina M. Lazov, Louise Lohse, Graham J. Belsham, Thomas Bruun Rasmussen, Anette Botner
Summary: This study compared the infectivity and immune response of two strains of PEDV from Germany and Italy in pigs. The German strain resulted in two peaks of virus excretion, while the Italian strain only had one peak. All pigs inoculated with either strain showed seroconversion after 18 and 10 days, respectively.
Article
Veterinary Sciences
Pei-Hua Wang, Ya-Qian Li, Yuan-Qing Pan, Yan-Yan Guo, Fan Guo, Rui-Zhu Shi, Li Xing
Summary: This study analyzed full-length spike genes of PEDVs isolated in China from 2007 to 2019 and identified 28 potential recombination events, leading to the emergence of a new evolutionary branch GI-c during 2016-2018. Amino acid variations in the spike protein could affect 20 potential linear B cell epitopes, demonstrating dramatic antigen drift in PEDVs circulating in China.
VETERINARY RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Hao Wang, Weicheng Yi, Huan Qin, Qin Wang, Rui Guo, Zishu Pan
Summary: In this study, a bivalent vaccine targeting classical swine fever virus (CSFV) and porcine epidemic diarrhea virus (PEDV) was successfully developed, demonstrating promising immunization effects and significant importance for the swine industry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Veterinary Sciences
Qi Gao, Zezhong Zheng, Heng Wang, Songqiang Yi, Guihong Zhang, Lang Gong
Summary: Emergency measures were taken at the start of the outbreak, and after vaccination with autogenous vaccines, the mortality rate of piglets in the farrowing house decreased, and production gradually stabilized. The virus sequence showed 97.8% homology with the vaccine strain, and vaccinated pigs exhibited satisfactory immune response.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Wan-Ting He, Nena Bollen, Yi Xu, Jin Zhao, Simon Dellicour, Ziqing Yan, Wenjie Gong, Cheng Zhang, Letian Zhang, Meng Lu, Alexander Lai, Marc A. Suchard, Xiang Ji, Changchun Tu, Philippe Lemey, Guy Baele, Shuo Su
Summary: The ongoing SARS-CoV-2 pandemic has revealed gaps in our understanding of animal coronaviruses, including the porcine epidemic diarrhea virus (PEDV). This study analyzed PEDV sequence data and identified Guangdong and Henan provinces in China as primary hubs for PEDV spread. The United States and China were found to have the highest number of PEDV lineages globally. It was estimated that the United States played a significant role in introducing PEDV to other countries through live swine trade. The findings highlight the importance of prevention and containment measures for livestock coronaviruses due to the global trade of live swine.
MOLECULAR BIOLOGY AND EVOLUTION
(2022)
Article
Virology
Gyu-Nam Park, Sok Song, SeEun Choe, Jihye Shin, Byung-Hyun An, Song-Yi Kim, Bang-Hun Hyun, Dong-Jun An
Summary: This study analyzed cases of porcine epidemic diarrhea (PED) in Korea from 2013 to 2022, confirming the presence of G2b and G2a genotypes, with G2b becoming dominant after 2020. The study also observed regional differences in PEDV prevalence and described the substitution rates and changes in population size in different regions.
Article
Infectious Diseases
Fangzhou Wu, Roger Cochrane, Joseph Yaros, Caixia Zhang, Shih-Yi Tsai, Gordon Spronk
Summary: Research has revealed that feed and feed ingredients can be potential routes of transmitting viral pathogens into swine farms. Multiple interventions, such as simplifying diet formulation to exclude high-risk ingredients and extending thermal treatment during pellet conditioning, have been implemented to lower viral spread via feed, resulting in a decrease in PED-related disease prevalence and PEDV-positive feed samples.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Virology
Chang Li, Hongyu Lu, Chao Geng, Keli Yang, Wei Liu, Zewen Liu, Fangyan Yuan, Ting Gao, Shuangshuang Wang, Ping Wen, Haofei Song, Yongxiang Tian, Danna Zhou
Summary: This study investigated swine enteric viruses in pig farms in south-central China and found that PEDV is the predominant pathogen causing piglet diarrhea. PEDV infection occurs mainly in relatively cold winter and spring in Hunan and Hubei provinces. The positive rate of PEDV showed an abnormal increase from 2020 to 2021, and that of PoRVA and PDCoV exhibited gradual increases from 2018 to 2021. PEDV-PoRVA and PEDV-PDCoV were the dominant co-infection modes. Genetic evolution analysis revealed that the PEDV GII-a is currently the epidemic genotype, and DY2020 has a different clade compared to other GII-a strains. The results emphasize the importance of strengthening surveillance and technical reserves for the prevention and control of piglet diarrhea.
Article
Microbiology
Dongliang Li, Yongtao Li, Yunchao Liu, Yumei Chen, Wenqiang Jiao, Hua Feng, Qiang Wei, Jucai Wang, Yuhang Zhang, Gaiping Zhang
Summary: Porcine epidemic diarrhea virus (PEDV) is a major pathogen causing diarrhea and high mortality in newborn piglets, with recombination and mutation being the main driving forces of its genetic evolution and diversity. A recent study identified a novel PEDV isolate (HNAY) with unique insertions and mutations in its S1 gene, suggesting potential differences in pathogenicity compared to other strains. The findings contribute to a better understanding of PEDV genetic evolution and molecular pathogenesis.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Xueying Han, Yangkun Liu, Yan Wang, Tiejun Wang, Ning Li, Feng Hao, Lunguang Yao, Kangkang Guo
Summary: In this study, a PEDV strain, designated PEDV WMB, was isolated and characterized from piglets with severe diarrhea in China. Whole-genome sequencing revealed that the strain belongs to subtype G2c and has a unique continuous mutation in the S1(0) antigenic epitope. The pathogenicity of PEDV WMB was confirmed in 3-day-old piglets, highlighting the importance of monitoring the genetic diversity of the virus and developing effective prevention measures.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Virology
Mingxia Li, Liping Zhang, Li Pan, Peng Zhou, Ruiming Yu, Zhongwang Zhang, Jianliang Lv, Huichen Guo, Yonglu Wang, Sa Xiao, Xinsheng Liu
Summary: In this study, the researchers found that nicotinamide (NAM) effectively inhibits the replication of PEDV and PDCoV by primarily targeting their RNA and protein synthesis processes. The activation of the ERK1/2/MAPK pathway and downregulation of transcription factors also contribute to the inhibition of virus replication. This suggests that NAM could be a promising antiviral candidate not only for swine intestinal coronaviruses but also for other human and animal coronaviruses.
Article
Virology
Chithra Sreenivasan, Milton Thomas, Zizhang Sheng, Ben M. Hause, Emily A. Collin, David E. B. Knudsen, Angela Pillatzki, Eric Nelson, Dan Wang, Radhey S. Kaushik, Feng Li
JOURNAL OF VIROLOGY
(2015)
Article
Biotechnology & Applied Microbiology
Lok R. Joshi, Maureen H. V. Fernandes, Travis Clement, Steven Lawson, Angela Pillatzki, Talita P. Resende, Fabio A. Vannucci, Gerald F. Kutish, Eric A. Nelson, Diego G. Diel
JOURNAL OF GENERAL VIROLOGY
(2016)
Article
Biotechnology & Applied Microbiology
Kyle S. Hain, Lok R. Joshi, Faten Okda, Julie Nelson, Aaron Singrey, Steven Lawson, Mathias Martins, Angela Pillatzki, Gerald F. Kutish, Eric A. Nelson, Eduardo F. Flores, Diego G. Diel
JOURNAL OF GENERAL VIROLOGY
(2016)
Article
Virology
Alexandria Van Noort, April Nelsen, Angela E. Pillatzki, Diego G. Diel, Feng Li, Eric Nelson, Xiuqing Wang
Article
Environmental Sciences
Angela E. Pillatzki, Regg D. Neiger, Steven R. Chipps, Kenneth F. Higgins, Nancy Thiex, Alan D. Afton
ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
(2011)
Article
Veterinary Sciences
Gregory W. Stevenson, Hai Hoang, Kent J. Schwartz, Eric R. Burrough, Dong Sun, Darin Madson, Vickie L. Cooper, Angela Pillatzki, Philip Gauger, Beverly J. Schmitt, Leo G. Koster, Mary L. Killian, Kyoungjin J. Yoon
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2013)
Article
Microbiology
D. M. Madson, D. R. Magstadt, P. H. E. Arruda, H. Hoang, D. Sun, L. P. Bower, M. Bhandari, E. R. Burrough, P. C. Gauger, A. E. Pillatzki, G. W. Stevenson, B. L. Wilberts, J. Brodie, K. M. Harmon, C. Wang, R. G. Main, J. Zhang, K. J. Yoon
VETERINARY MICROBIOLOGY
(2014)
Article
Veterinary Sciences
Kyle L. Tofflemire, R. David Whitley, David M. Wong, Kenneth R. Waller, Ronald K. Myers, Angela E. Pillatzki, Gil Ben-Shlomo
VETERINARY OPHTHALMOLOGY
(2013)
Review
Pathology
R. E. Sacco, J. L. McGill, A. E. Pillatzki, M. V. Palmer, M. R. Ackermann
VETERINARY PATHOLOGY
(2014)
Article
Virology
Xufang Deng, Albert van Geelen, Alexandra C. Buckley, Amornrat O'Brien, Angela Pillatzki, Kelly M. Lager, Kay S. Faaberg, Susan C. Baker
JOURNAL OF VIROLOGY
(2019)
Article
Immunology
Gagandeep Singh, Oksana Zholobko, Angela Pillatzki, Brett Webb, Eric Nelson, Andriy Voronov, Sheela Ramamoorthy
Article
Veterinary Sciences
Oleksandr Kolyvushko, Agm Rakibuzzaman, Angela Pillatzki, Brett Webb, Sheela Ramamoorthy
VETERINARY SCIENCES
(2019)
Article
Veterinary Sciences
Angela E. Pillatzki, Phillip C. Gauger, Darin M. Madson, Eric R. Burrough, Jianqiang Zhang, Q. Chen, Drew R. Magstadt, Paulo H. E. Arruda, Greg W. Stevenson, Kyoungjin J. Yoon
JOURNAL OF SWINE HEALTH AND PRODUCTION
(2015)
Meeting Abstract
Biochemistry & Molecular Biology
Nicole Cray, Angela Pillatzki, Yinsheng Zhao, Diane F. Birt, Elizabeth M. Whitley