Article
Immunology
Hsiu-Hsien Lin, Kun-Hao Tseng, Ni Tien, Yu-Tzu Lin, Jiaxin Yu, Po-Ren Hsueh, Der-Yang Cho
Summary: The rapid Sepsityper protocol based on the MALDI Biotyper platform accurately identifies the species of microorganisms in blood culture samples. Gram-negative bacilli have a higher identification rate compared to Gram-positive cocci and yeasts. However, the identification performance of the rapid Sepsityper protocol is poor for polymicrobial samples.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2022)
Article
Microbiology
Agustina Costa, Roque Figueroa-Espinosa, Jerson A. A. Martinez, Liliana Fernandez-Canigia, Maria I. Maldonado, Silvina A. A. Bergese, Ana E. E. Schneider, Carlos Vay, Carlos H. H. Rodriguez, Marcela Nastro, Gabriel O. O. Gutkind, Jose A. Di Conza
Summary: Carbapenemase resistance in Enterobacterales is a global public health problem. This study evaluated a protocol for detecting Klebsiella pneumoniae carbapenemase (KPC) using MALDI-TOF MS technology. The KPC direct detection showed a sensitivity and specificity of 100% from patients' positive blood cultures, short-term cultures, and colonies. This study is significant in the context of multi-resistance and highlights the need for actions to reduce the spread of antibiotic resistance.
Article
Biology
Gabrijela Perse, Ivana Samoscanec, Zrinka Bosnjak, Ana Budimir, Tomislav Kulis, Ivana Marekovic
Summary: This study evaluated an in-house method for identifying bacteria directly from positive blood cultures, compared to the Sepsityper (R) Kit, and assessed the benefit of on-plate formic acid extraction. The results showed that the in-house method had lower identification rates for Gram-positive bacteria at the genus and species level, but the addition of on-plate formic acid extraction significantly improved the identification rate.
Article
Infectious Diseases
Noriyuki Watanabe, Sachie Koyama, Yoshitada Taji, Kotaro Mitsutake, Yasuhiro Ebihara
Summary: In this study, the performance of the Rapid Sepsityper Kit in species identification and antimicrobial susceptibility testing in blood culture samples was evaluated. The direct method using the kit showed good results in species identification, especially for Gram-negative bacilli. In antimicrobial susceptibility testing, the direct method also showed high categorical agreement, but there were still some misidentifications among streptococcus species.
JOURNAL OF INFECTION AND CHEMOTHERAPY
(2022)
Article
Infectious Diseases
Richard D. Smith, J. Kristie Johnson, Robert K. Ernst
Summary: Fast and accurate diagnostics are crucial for managing bloodstream infections. This study compared three different diagnostic platforms and found that BioFire BCID2 performed the best, providing accurate identification of pathogens in the shortest amount of time.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2023)
Article
Microbiology
Hazan Zengin Canalp, Banu Bayraktar
Summary: This study investigated the accuracy and feasibility of a rapid identification method for blood pathogens and found that the RID method significantly reduced the turnaround time compared to the SIRID method, making it a valuable tool for patient management.
MICROBIOLOGY SPECTRUM
(2021)
Article
Agronomy
Jadwiga Stanek-Tarkowska, Milosz Pastuszczak, Ewa Szpunar-Krok, Miroslava Kacaniova, Maciej Ireneusz Kluz, Ewa Antonina Czyz, Rafal Pieniazek, Karol Skrobacz, Karol Pietrzyk
Summary: The aim of the research was to investigate the impact of different doses of ash from biomass combustion on the number and community of soil bacteria. The study found that the use of biomass ash as fertilizer led to an increase in the number of microorganisms, particularly at doses ranging from 200 to 500 kg K-2 Oha(-1). Soil moisture, in addition to ash dosage, was identified as an important factor in bacterial growth. The highest amount of soil bacteria was observed in these variants, and a total of 44 bacterial species belonging to 5 genera were identified.
Article
Food Science & Technology
Eiseul Kim, Seung-Min Yang, Hae-Yeong Kim
Summary: This study investigates the cultivable microbial community in kimchi fermented at different temperatures using MALDI-TOF MS, revealing the impact of fermentation temperature on the quality of kimchi and the competitive relationship between different lactic acid bacteria species during kimchi fermentation.
Article
Multidisciplinary Sciences
Song Li, Dexing Han, Xiaowei Chen, Dexiang Zheng, Yimei Cai, Dongling Lin, Xuan Zhang, Peifeng Ke, Pinghua Qu, Cha Chen
Summary: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is widely used in clinical microbiology labs for diagnostic purposes. This study evaluated the performance of the Zybio EXS3000 in microbial identification compared to VITEK MS. The results showed that both devices provided accurate diagnostic results, but the EXS3000 system needs improvement in identifying Candida albicans.
Article
Microbiology
Andres Perez-Lopez, Nazik Elamin, Rhanty Nabor, Sarah Dumindin, Diane Roscoe, Mohammad Rubayet Hasan, Mohammed Suleiman, Patrick Tang
Summary: The study demonstrated the performance and early therapeutic impact of the in-house method for direct identification of bloodstream pathogens in pediatric blood culture bottles by matrix-assisted laser desorption ionization-time of flight mass spectrometry. DIMT accurately identified pathogens, led to therapeutic modifications in patients with bloodstream infections, especially those with intrinsic antibiotic resistance, and assisted in the early confirmation of contaminants in blood cultures.
MICROBIOLOGY SPECTRUM
(2021)
Article
Infectious Diseases
Yuliya Zboromyrska, Veronica Rico, Cristina Pitart, Mariana Jose Fernandez-Pittol, Alex Soriano, Jordi Bosch
Summary: The direct identification and rapid detection of ESBL and carbapenemase-producing strains from urine samples is a reliable and useful tool in adjusting empirical antimicrobial treatment.
Article
Microbiology
Romain Lotte, Audrey Courdurie, Alice Gaudart, Audrey Emery, Alicia Chevalier, Albert Tran, Mathilde Payen, Raymond Ruimy
Summary: Direct identification of bacteria in ascitic fluid can improve the management of patients with spontaneous bacterial peritonitis by facilitating early introduction of appropriate antibiotic therapy.
Article
Agriculture, Dairy & Animal Science
Alina Buchilina, Kayanush Aryana
Summary: Camel milk and monk fruit sweetener both contain compounds that are beneficial for human health, with the former being used for treating allergies, diabetes, and autism, and the latter being a natural, 0-calorie sweetener that helps prevent oxidation and cancer.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Medicine, General & Internal
Inaki Beguiristain, Lucia Henriquez, Ignacio Sancho, Carmen Martin, Angel Hidalgo-Ovejero, Carmen Ezpeleta, Maria Eugenia Portillo
Summary: The accurate and fast diagnosis of prosthetic joint infection is crucial. This study assesses the role of direct MALDI-TOF MS for early identification of pathogens causing PJI from sonication fluid inoculated in blood culture bottles.
Article
Infectious Diseases
Natalia Kehl Moreira, Camila Morschbacher Wilhelm, Ayme Duarte Echevarria, Fabiana Caroline Zempulski Volpato, Priscila Lamb Wink, Afonso Luis Barth, Juliana Caierao
Summary: Detection of KPC enzyme directly from positive blood cultures using MALDI-TOF MS is feasible and has high sensitivity and specificity. This method can rapidly and accurately assess bacterial resistance mechanisms, which is crucial for selecting appropriate treatment and improving antimicrobial stewardship.
Article
Pathology
Blake W. Buchan, Katherine M. Riebe, Markus Timke, Markus Kostrzewa, Nathan A. Ledeboer
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
(2014)
Review
Microbiology
Blake W. Buchan, Nathan A. Ledeboer
CLINICAL MICROBIOLOGY REVIEWS
(2014)
Article
Immunology
Tyler K. Ulland, Ann M. Janowski, Blake W. Buchan, Matthew Faron, Suzanne L. Cassel, Bradley D. Jones, Fayyaz S. Sutterwala
INFECTION AND IMMUNITY
(2013)
Article
Microbiology
Neil W. Anderson, Blake W. Buchan, Katherine M. Riebe, Lauren N. Parsons, Stacy Gnacinski, Nathan A. Ledeboer
JOURNAL OF CLINICAL MICROBIOLOGY
(2012)
Article
Microbiology
Blake W. Buchan, Wendy J. Olson, Michael Pezewski, Mario J. Marcon, Thomas Novicki, Timothy S. Uphoff, Lakshmi Chandramohan, Paula Revell, Nathan A. Ledeboer
JOURNAL OF CLINICAL MICROBIOLOGY
(2013)
Article
Microbiology
Neil W. Anderson, Blake W. Buchan, Carol L. Young, Duane W. Newton, Connie Brenke, Linda Lapsley, Paul A. Granato, Nathan A. Ledeboer
JOURNAL OF CLINICAL MICROBIOLOGY
(2013)
Article
Microbiology
Neil W. Anderson, Blake W. Buchan, Donna Mayne, Joel E. Mortensen, Tami-Lea A. Mackey, Nathan A. Ledeboer
JOURNAL OF CLINICAL MICROBIOLOGY
(2013)
Article
Microbiology
Karen C. Carroll, Blake W. Buchan, Sokha Tan, Paul D. Stamper, Katherine M. Riebe, Preeti Pancholi, Cheryl Kelly, Arundhati Rao, Robert Fader, Robert Cavagnolo, Wendy Watson, Richard V. Goering, Ernest A. Trevino, Alice S. Weissfeld, Nathan A. Ledeboer
JOURNAL OF CLINICAL MICROBIOLOGY
(2013)
Review
Microbiology
Blake W. Buchan, Nathan A. Ledeboer
JOURNAL OF CLINICAL MICROBIOLOGY
(2013)
Review
Microbiology
Neil W. Anderson, Blake W. Buchan, Nathan A. Ledeboer
JOURNAL OF CLINICAL MICROBIOLOGY
(2014)
Article
Microbiology
Blake W. Buchan, Wendy J. Olson, Tami-Lea A. Mackey, Nathan A. Ledeboer
JOURNAL OF CLINICAL MICROBIOLOGY
(2014)
Article
Microbiology
Eric T. Beck, Blake W. Buchan, Katherine M. Riebe, Brenda R. Alkins, Preeti Pancholi, Paul A. Granato, Nathan A. Ledeboer
JOURNAL OF CLINICAL MICROBIOLOGY
(2014)
Article
Microbiology
Neil W. Anderson, Blake W. Buchan, Nathan A. Ledeboer
JOURNAL OF CLINICAL MICROBIOLOGY
(2014)
Article
Medicine, General & Internal
Blake W. Buchan, Christine C. Ginocchio, Ryhana Manii, Robert Cavagnolo, Preeti Pancholi, Lettie Swyers, Richard B. Thomson, Christopher Anderson, Karen Kaul, Nathan A. Ledeboer