Article
Veterinary Sciences
Ying Shi, Benqiang Li, Jie Tao, Jinghua Cheng, Huili Liu
Summary: This study found that various viruses caused severe diarrhea outbreaks in pig populations, with PKoV, PAstV, and PEDV being the most common viruses with a high infection rate. Multiple infections were common, and infection patterns varied among different pig farms.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Microbiology
Hannah Tyrrell, Sarah B. N. Morin, Colin D. Lloyd, Brendon Parsons, Taryn Stokowski, Jianling Xie, Ran Zhuo, Bonita E. Lee, Xiao-Li Pang, Stephen B. Freedman, Linda Chui
Summary: Detection of Clostridioides difficile toxins using enteric multiplex syndromic panels has been compared to direct-from-stool (DFS) and culture-enriched stools followed by polymerase chain reaction (PCR) methods in pediatric populations. The study found excellent agreement between the Luminex xTAG Gastrointestinal Pathogen Panel (GPP) and the DFS in-house real-time PCR (DFS RT-PCR) assay, with a high positive and negative percent agreement. GPP can be easily implemented in routine diagnostic laboratories to detect enteric pathogens, including C. difficile.
Article
Infectious Diseases
Greta Roncarati, Claudio Foschi, Simone Ambretti, Maria Carla Re
Summary: The study aimed to evaluate a rapid method for identifying Gram-negative bacteria from positive blood cultures, detecting ESβL and carbapenemases production using MALDI-TOF/MS analysis. The proposed protocol successfully provided reliable bacterial identification and data on β-lactam resistance in just 3 hours, improving patient management in terms of antimicrobial stewardship.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Article
Medicine, General & Internal
Li-Min Xie, Xin Yin, Tian-Ao Xie, Jian-Wen Su, Qin Huang, Jing-Hao Zhang, Yin-Fei Huang, Xu-Guang Guo
Summary: This study evaluated the diagnostic efficacy of the Luminex xTAG Respiratory Virus Panel (RVP) FAST v2 test on respiratory viral infections. Results showed that the test had high specificity and sensitivity, making it a reliable and rapid diagnostic method for multiple respiratory viral infections.
YONSEI MEDICAL JOURNAL
(2022)
Article
Multidisciplinary Sciences
Arpasiri Srisrattakarn, Pimchanok Panpru, Patcharaporn Tippayawat, Aroonwadee Chanawong, Ratree Tavichakorntrakool, Jureerut Daduang, Lumyai Wonglakorn, Aroonlug Lulitanond
Summary: A rapid and highly sensitive recombinase polymerase amplification-lateral flow strip (RPA-LF) method was developed and evaluated for direct detection of methicillin-resistant Staphylococcus aureus (MRSA). This method showed high specificity and sensitivity, and has the potential for wide application without the need for special equipment.
Article
Oncology
Cuipeng Qiu, Xiao Wang, Serina A. Batson, Bofei Wang, Carlos A. Casiano, Giulio Francia, Jian-Ying Zhang
Summary: This study utilized Luminex technology to evaluate and optimize a panel of anti-tumor associated antigen (TAA) autoantibodies for prostate cancer (PCa) detection. Twelve autoantibodies showed significantly high frequencies in PCa group, and the performance of HSP60 autoantibody was confirmed using ELISA. A panel consisting of p16, IMP2, and HSP60 achieved an impressive AUC of 0.910 with high sensitivity and specificity, particularly in distinguishing Hispanic American PCa patients from normal controls.
Article
Microbiology
Hanshu Fang, Chung-Ho Lee, Huiluo Cao, Shuo Jiang, Simon Yung-Chun So, Cindy Wing-Sze Tse, Vincent Chi-Chung Cheng, Pak-Leung Ho
Summary: The study evaluated the NG-Test(R) CTX-M MULTI lateral flow assay (CTX-M LFA) for rapid detection of CTX-M producers in spiked and clinical blood cultures. The results showed that the CTX-M LFA could accurately detect all CTX-M producers in spiked blood cultures, but false-positive results were observed in clinical blood cultures. Therefore, the CTX-M LFA can provide a reliable and rapid detection method for CTX-M producers in blood cultures.
Article
Microbiology
Jacqueline Babb, Audra Clark, Donna Gaffney, Kareem Abdelfattah, Bonnie C. Prokesch
Summary: This study demonstrates that regular aerobic and anaerobic blood culture sets are sufficient in detecting the most common causes of fungemia, and thus, sending fungal blood cultures for patients in surgical and burn intensive care units is not a good use of resources.
MICROBIOLOGY SPECTRUM
(2022)
Article
Chemistry, Multidisciplinary
Saverio Monica, Elena Bancalari, Vincenzo Castellone, Jos Rijkx, Sarah Wirth, Anika Jahns, Benedetta Bottari
Summary: The study aimed to detect bacteria and yeasts in wines rapidly using a bioluminescence assay protocol. The developed protocol successfully discriminated the presence of yeasts and bacteria in different types of wines within 24 hours, down to approximately 50 CFU/L of yeasts and 1000 CFU/L of bacteria. Industries would benefit from this early detection and discrimination of microbial contaminants for decision-making processes.
APPLIED SCIENCES-BASEL
(2021)
Article
Chemistry, Analytical
Sishuo Song, Fan Ji, Qingdong Zeng, Xiaoteng Ding, Jiahao Lin, Xinsheng Wang, Yuanhong Xu, Ruijie Deng, Jinghong Li
Summary: Fungicide abuse can lead to the emergence of fungicide-resistant fungal pathogens, posing a threat to agriculture and food safety. In this study, researchers developed iARMS, an isothermal amplification refractory mutation system, which enables rapid and sensitive detection of fungicide-resistant crop fungal pathogens. The iARMS assay showed high specificity and sensitivity, and it was able to detect rare fungicide-resistant isolates.
ANALYTICAL CHEMISTRY
(2023)
Article
Infectious Diseases
Nicole L. Belanger, Su Jeoung Kim, Paulo J. M. Bispo
Summary: Candida species are the most common pathogens responsible for ocular infections in temperate regions of the world. This study sequenced and molecularly characterized Candida species isolated from patients with eye infections to understand their distribution over time.
Article
Biology
Taishi Yasukawa, Arisa H. Oda, Takahiro Nakamura, Naohisa Masuo, Miki Tamura, Yuriko Yamasaki, Makoto Imura, Takatomi Yamada, Kunihiro Ohta
Summary: The TAQing2.0 system enables the delivery of endonucleases into the cell nucleus of asexual industrial microorganisms, inducing genomic rearrangements and generating mutants with altered phenotypes, without the need for introducing foreign DNA.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
S. W. Peterson, W. Demczuk, I. Martin, H. Adam, A. Bharat, M. R. Mulvey
Summary: Bloodstream infections are a significant global health concern. Utilizing whole genome sequencing can help accurately identify and characterize pathogens, allowing for prompt initiation of appropriate antimicrobial therapies and prevention of antimicrobial resistance.
Article
Biochemical Research Methods
Pedro A. Baldera-Aguayo, Arden Lee, Virginia W. Cornish
Summary: Antibiotic resistance is a global health threat, and developing new tetracycline analogue therapeutics is urgent. By engineering yeast, researchers have successfully produced the fungal tetracycline TAN-1612 and significantly increased its yield. This study marks an important step in combating antibiotic resistance.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Public, Environmental & Occupational Health
Soo Kai Ter, Sayaphet Rattanavong, Tamalee Roberts, Amphonesavanh Sengduangphachanh, Somsavanh Sihalath, Siribun Panapruksachat, Manivanh Vongsouvath, Paul N. Newton, Andrew J. H. Simpson, Matthew T. Robinson
Summary: The study found that molecular investigation of negative blood cultures can identify potential bacterial pathogens that routine culture may miss. This method could potentially impact patient management and further research is needed to determine its cost-benefit, implementation, and timing.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2021)
