Article
Engineering, Electrical & Electronic
Tom R. Molderez, Korneel Rabaey, Marian Verhelst
Summary: In this study, a high-speed potentiostat with 128 individual channels was proposed, utilizing a channel architecture exploiting time division multiplexing to minimize hardware cost and area. Through parallelism, chronoamperometry experiments can be conducted 128 times faster, while sampling instead of voltage sweeping enables a 72 times faster operation of a conventional 1 mV s-1-Cyclic Voltammetry.
IEEE TRANSACTIONS ON CIRCUITS AND SYSTEMS I-REGULAR PAPERS
(2021)
Article
Chemistry, Multidisciplinary
Giang T. H. Nguyen, Jack L. Bennett, Sherrie Liu, Sarah E. Hancock, Daniel L. Winter, Dominic J. Glover, William A. Donald
Summary: The new method combines mass spectrometry with multistage, high-resolution native mass spectrometry to rapidly identify natural products that bind to therapeutically relevant protein targets, significantly increasing the efficiency of target-based natural product drug discovery workflows.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Analytical
Rebecca B. Clark, Matthew W. Glasscott, Matthew D. Verber, Jenna C. DeMartino, Anton Netchaev, Jason D. Ray, Eric W. Brown, Erik Alberts, P. U. Ashvin Iresh Fernando, Lee C. Moores, Jeffrey E. Dick
Summary: Three different potentiostat architectures for electrochemical measurements are discussed, with a focus on the advantages of using a multiplexer interface. A strategy for developing flexible printed circuit (FPC) electrode arrays for single-channel potentiostats is presented, along with experimental results. The FPC array is interfaced with commercial potentiostats using a simple connector and multiplexers, demonstrating reliability, stability, and reproducibility.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Yang Feng, Tingyan Chen, Qianli Rao, Xiaobo Xie, Yi Lv, Lichun Zhang
Summary: In this study, a binary encoding strategy based on persistent luminescence (PersL) lifetime/color was proposed for accurate diagnosis of SARS-CoV-2. By manipulating the lifetimes and emissions of PersL nanoplatforms, a temporal coding dimension was created, allowing simultaneous identification of multiple targets. The purpose-built time resolved PersL technology successfully decoded multiple populations of barcodes. This research provides a powerful tool for optical multiplexing in biomedical applications.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Divya Balakrishnan, Janwa El Maiss, Wouter Olthuis, Cesar Pascual Garcia
Summary: The control of acidity in the assembly of biopolymers is crucial for various applications. The miniaturization of a device containing multiplexed microreactors enables independent electrochemical control of acidity in very small volumes, with a large acidity range and high accuracy. The achieved performance in acidity control, miniaturization, and multiplexing paves the way for combinatorial chemistry through pH and acidity-controlled reactions.
Article
Materials Science, Multidisciplinary
Jose de la Cruz, Diep Nguyen, Xavi Illa, Jessica Bousquet, Antonio P. Perez-Marin, Elena del Corro, Serge Picaud, Jose A. Garrido, Clement Hebert
Summary: Electroretinography (ERG) is a clinical test used to understand and diagnose various retinopathies. Traditional metal electrodes have limitations, while flexible electrodes made of graphene can overcome these limitations and perform multisite recording on the cornea.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Biophysics
Diming Zhang, Yuting Xiang, Quchao Zou, Kai Zhu, Ning Hu
Summary: The assessment of drug cardiotoxicity using the electrical and mechanical properties of cardiomyocytes is crucial. Traditional recording strategies have limitations, but a new multimodal microelectrode biosensing system allows for simultaneous and dynamic monitoring of electromechanical signals from single cardiomyocytes.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Multidisciplinary Sciences
Leo Dahl, Ilana B. Kotliar, Annika Bendes, Tea Dodig-Crnkovic, Samuel Fromm, Arne Elofsson, Mathias Uhlen, Thomas P. Sakmar, Jochen M. Schwenk
Summary: A multiplexed immunoassay was developed to test the selectivity of over 400 anti-GPCR antibodies. The results showed that about 61% of the antibodies were selective, 11% bound off-target, and 28% did not bind to any GPCR. It was also found that on-target antibodies had longer, more disordered antigens and were less buried in the interior of GPCR proteins compared to other antibodies.
Article
Multidisciplinary Sciences
Andrea Spanu, Annalisa Bonfiglio
Summary: Modern electrophysiology has advanced due to the development of sophisticated tools and materials, with the emergence of organic bioelectronics offering new advancements in cellular interfacing.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Biochemistry & Molecular Biology
Miyu Okamoto, Kenta Shimba, Akihiro Kamono, Kiyoshi Kotani, Yasuhiko Jimbo
Summary: The study successfully developed a culture system to measure the spatial dynamics of reentry-like activity, revealing that a simple purification process is sufficient to induce reentry-like activity, which occurs mainly through changes in inter-beat intervals and conduction block for initiation and termination.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Zhichen Xu, Dongjuan Chen, Tao Li, Jiayu Yan, Jiang Zhu, Ting He, Rui Hu, Ying Li, Yunhuang Yang, Maili Liu
Summary: MiCaR is a nucleic acid testing platform that combines microfluidic devices, CRISPR-Cas12a, and multiplex recombinase polymerase amplification to detect multiple nucleic acid targets simultaneously. It shows excellent sensitivity and specificity in detecting HPV and respiratory viruses.
NATURE COMMUNICATIONS
(2022)
Article
Neurosciences
Jennifer Lawson, Elijah LaVancher, Mauricio DeAlmeida, Bryan James Black
Summary: Activated glia can have either neuroprotective or neurodegenerative effects in chronic pain regulation. Recent findings show that satellite glial cells and astrocytes, although not capable of generating action potentials, express voltage- and ligand-gated ion channels that allow measurable calcium transients and modulation of sensory neuron excitability. Researchers have developed a co-culture model using microelectrode arrays to simultaneously monitor the phenotypic activity of neurons and astrocytes, which may be valuable for identifying potential analgesics and compounds targeting glia-mediated pathologies.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Jin Song, Ashaq Ali, Yaohong Ma, Yiwei Li
Summary: In this study, a microfluidic system using a graphene-modified microelectrode array sensor was proposed for real-time monitoring of bacterial biofilms through electrochemical impedance. The results showed that increasing bacterial cell load on the sensing interface significantly prolonged the observation window period of the biofilm state, thereby improving the sensing signal quality. Simulation studies demonstrated that this method outperformed traditional endpoint detection in anti-biofilm drug screening.
NANOSCALE ADVANCES
(2023)
Article
Cell Biology
YoungJu Jo, Hyungjoo Cho, Wei Sun Park, Geon Kim, DongHun Ryu, Young Seo Kim, Moosung Lee, Sangwoo Park, Mahn Jae Lee, Hosung Joo, HangHun Jo, Seongsoo Lee, Sumin Lee, Hyun-seok Min, Won Do Heo, YongKeun Park
Summary: Jo et al. develop a broadly applicable deep-learning approach to predict fluorescence (FL) based on label-free refractive index (RI) measurements, 'RI2FL' (RI to FL). The trained model can be used across cell types without retraining.
NATURE CELL BIOLOGY
(2021)
Article
Engineering, Biomedical
Tianshu Dong, Lei Chen, Paras R. Patel, Julianna M. Richie, Cynthia A. Chestek, Albert J. Shih
Summary: The machine described in this study automates the assembly of high-density carbon fiber (HDCF) arrays, eliminating the need for manual labor and improving accuracy and repeatability. The machine includes a roller-based carbon fiber extruder, a motion system with linear and rotary stages, an imaging system with digital microscope cameras, and a laser cutter. It is capable of precisely handling carbon fiber electrodes (CFEs) and accurately placing them on silicon support shanks. The use of this machine is significant for achieving fully automated HDCF array assembly and batch production.
JOURNAL OF NEURAL ENGINEERING
(2023)
Meeting Abstract
Neurosciences
Timothy Shafer
NEUROTOXICOLOGY AND TERATOLOGY
(2015)
Article
Pharmacology & Pharmacy
Jenna D. Strickland, William R. LeFew, James Crooks, Diana Hall, Jayna N. R. Ortenzio, Kevin Dreher, Timothy J. Shafer
Article
Neurosciences
Andrew F. M. Johnstone, Jenna D. Strickland, Kevin M. Crofton, Chris Gennings, Timothy J. Shafer
Article
Toxicology
Christopher L. Frank, Jasmine P. Brown, Kathleen Wallace, William R. Mundy, Timothy J. Shafer
TOXICOLOGICAL SCIENCES
(2017)
Article
Neurosciences
Pablo Valdivia, Matt Martin, William R. LeFew, James Ross, Keith A. Houck, Timothy J. Shafer
Article
Neurosciences
Cina M. Mack, Bryant J. Lin, James D. Turner, Andrew F. M. Johnstone, Lyle D. Burgoon, Timothy J. Shafer
Article
Neurosciences
Stephen R. Lantz, Cina M. Mack, Kathleen Wallace, Ellen F. Key, Timothy J. Shafer, John E. Casida
Article
Toxicology
Magdalini Sachana, Anna Bal-Price, Kevin M. Crofton, Susanne H. Bennekou, Timothy J. Shafer, Mamta Behl, Andrea Terron
TOXICOLOGICAL SCIENCES
(2019)
Article
Toxicology
Timothy J. Shafer, Jasmine P. Brown, Brittany Lynch, Sylmarie Davila-Montero, Kathleen Wallace, Katie Paul Friedman
TOXICOLOGICAL SCIENCES
(2019)
Article
Toxicology
Marissa B. Kosnik, Jenna D. Strickland, Skylar W. Marvel, Dylan J. Wallis, Kathleen Wallace, Ann M. Richard, David M. Reif, Timothy J. Shafer
ARCHIVES OF TOXICOLOGY
(2020)
Article
Toxicology
Lorena Saavedra, Kathleen Wallace, Theresa F. Freudenrich, Moritz Mall, William R. Mundy, Jorge Davila, Timothy J. Shafer, Marius Wernig, Daniel Haag
Summary: This study established highly functional neuron/glia cocultures using excitatory and inhibitory neurons directly reprogrammed from hiPSCs and primary human astrocytes, demonstrating their utility for functional neurotoxicity screening of environmental chemicals. Acute changes in network activity in response to compounds with known adverse effects were observed, showing concentration-dependent effects on multiple neural network activity metrics using both human neuron/glia cocultures and rat primary cortical cultures.
TOXICOLOGICAL SCIENCES
(2021)
Article
Toxicology
Carmen A. Marable, Christopher L. Frank, Roland F. Seim, Susan Hester, W. Matthew Henderson, Brian Chorley, Timothy J. Shafer
Summary: This study investigated the signaling pathways and physiological regulators underlying chemically induced neural network dysfunction. The results showed that different classes of compounds produce different responses and changes in network formation are accompanied by transcriptomic and metabolomic changes.
TOXICOLOGICAL SCIENCES
(2022)
Article
Toxicology
Kelly E. Carstens, Amy F. Carpenter, Melissa M. Martin, Joshua A. Harrill, Timothy J. Shafer, Katie Paul Friedman
Summary: In vivo developmental neurotoxicity testing is resource intensive and lacks information on cellular processes affected by chemicals. This study evaluates new approach methodologies for DNT and finds that the DNT NAM battery provides a sensitive biomarker for DNT bioactivity, particularly in cytotoxicity and network connectivity parameters. Selective bioactivity provides insight into specific functional processes affected by chemical exposure.
TOXICOLOGICAL SCIENCES
(2022)
Article
Toxicology
Jenna D. Strickland, Matthew T. Martin, Ann M. Richard, Keith A. Houck, Timothy J. Shafer
ARCHIVES OF TOXICOLOGY
(2018)