Article
Multidisciplinary Sciences
Yue-Mei Hu, Zhao-Feng Bi, Ya Zheng, Li Zhang, Feng-Zhu Zheng, Kai Chu, Ya-Fei Li, Qi Chen, Jia-Li Quan, Xiao-Wen Hu, Xing-Cheng Huang, Kong-Xin Zhu, Ya-Hui Wang-Jiang, Han -Min Jiang, Xia Zang, Dong-Lin Liu, Chang -Lin Yang, Hong-Xing Pan, Qiu-Fen Zhang, Ying-Ying Su, Shou-Jie Huang, Guang Sun, Wei-Jin Huang, Yue Huang, Ting Wu, Jun Zhang, Ning-Shao Xia
Summary: This study evaluated the immunogenicity and safety of the Escherichia coli-produced second-generation nonavalent HPV vaccine (Cecolin 9). The results showed that Cecolin 9 had good immunogenicity against all nine HPV types and was well tolerated.
Article
Biotechnology & Applied Microbiology
Yuee Zu, Zhihua Ou, Dan Wu, Wei Liu, Liwen Liu, Di Wu, Yanping Zhao, Peidi Ren, Yanqing Zhang, Wangsheng Li, Shujin Fu, Yongchun Wen, Xianchu Cai, Wenbo Liao, Chunyu Geng, Hongcheng Zhou, Xiaman Wang, Haorong Lu, Huanhuan Peng, Na Liu, Shida Zhu, Jiyang Liu, Dongbo Wang, Junhua Li
Summary: This study collected cervical exfoliated cell samples from females in Changsha city, Hunan Province to investigate the distribution of four major HPV types in China. The lineage distribution of HPV 16, 18, 52, and 58 was confirmed, with the identification of potentially high oncogenic sublineages. The findings contribute valuable evidence for HPV DNA screening, vaccine effectiveness evaluation, and control strategy development.
Article
Immunology
Kavita Panwar, Anna Godi, Clementina E. Cocuzza, Nick Andrews, Jo Southern, Paul Turner, Elizabeth Miller, Simon Beddows
Summary: The study evaluated the antibody response of HPV vaccines against different types of human papillomavirus. The findings showed that the vaccines provided long-lasting protection against vaccine types and maintained high antibody levels against related non-vaccine types. This contributes to the evidence base supporting public health intervention.
Article
Health Care Sciences & Services
Kai Chu, Zhao-Feng Bi, Wei-Jin Huang, Ya-Fei Li, Li Zhang, Chang-Lin Yang, Han -Min Jiang, Xia Zang, Qi Chen, Dong-Lin Liu, Hong-Xing Pan, Yue Huang, Feng-Zhu Zheng, Qiu-Fen Zhang, Guang Sun, Ying-Ying Su, Shou-Jie Huang, Hui-Rong Pan, Ting Wu, Yue-Mei Hu, Jun Zhang, Feng-Cai Zhu, Ning-Shao Xia
Summary: This phase 1 clinical trial evaluated the safety and immunogenicity of a second-generation nonavalent HPV vaccine and showed that it was well tolerated and immunogenic, providing encouragement for further studies in larger cohorts and a wider age range.
LANCET REGIONAL HEALTH-WESTERN PACIFIC
(2023)
Article
Virology
Kohsei Tanaka, Gota Kogure, Mamiko Onuki, Koji Matsumoto, Takashi Iwata, Daisuke Aoki, Iwao Kukimoto
Summary: This study analyzed the complete genome sequences of HPV16, HPV18, and HPV58 and found Japan-specific clusters in the phylogenetic analysis. The estimated timeframe suggests that these HPV variants were introduced to Japan at least 25,000 years ago, indicating a co-migration scenario with ancestral Japanese populations during the Upper Paleolithic period.
Article
Infectious Diseases
Sayamon Hongjaisee, Natteewan Doungjinda, Woottichai Khamduang, Tanawan Samleerat Carraway, Jiraprapa Wipasa, Jose D. Debes, Khuanchai Supparatpinyo
Summary: The study developed a reverse transcription loop-mediated isothermal amplification (RTLAMP) assay for detecting hepatitis C virus (HCV) in a single closed tube. The assay showed high sensitivity, specificity, and stability in detecting different HCV genotypes, making it a potentially useful tool for identifying individuals in need of HCV treatment, particularly in resource-limited settings.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Chemistry, Analytical
Huan-Huan Zhu, Yuan Li, Li-Xia Wu, Ke-Sheng Wang, Yue Zhang, Qiang-Yuan Fan, Zun-Zhen Ming, Wei-Qin Chen, Wei-Wei Liu
Summary: Loop-mediated isothermal amplification (LAMP) is a promising diagnostic tool for genetic amplification, known for its speed, simplicity, efficiency, and sensitivity. This research introduces an internal heating method for LAMP using magnetic nanoparticles (MNPs) called nano-LAMP, which can detect low concentrations of HPV and has been validated in clinical samples.
Article
Chemistry, Analytical
Qi Chen, Li Yao, Qian Wu, Jianguo Xu, Chao Yan, Chuanxiang Guo, Chao Zhang, Tao Xu, Panzhu Qin, Wei Chen
Summary: A biosensor based on a polymerase chain reaction (PCR) integrated lateral flow strip platform was developed for the rapid and simultaneous visual identification of high-risk human papillomavirus (HPV) genotypes 16 and 18 in clinical samples. This biosensor utilized a one-step protocol for nucleic acid extraction and designed functionalized primer sets specific to HPV-16 and 18 for simultaneous amplification. By combining PCR amplification efficiency and the visualized sensing of the lateral flow strip (LFS), this method demonstrated the ability to detect as low as 700 copies of HPV-16 and 18 DNA within 75 minutes. This research has the potential to promote application in resource limited settings.
Article
Multidisciplinary Sciences
Khalid Al-Shibli, Hiba Abdul Latif Mohammed, Ramona Maurseth, Mikkel Fostervold, Sebastian Werner, Sveinung Wergeland Sorbye
Summary: The study aimed to investigate the detection rate of CIN3+ in women aged 25-39 with normal cytology by using a 3-type HPV mRNA test as a targeted quality assurance measure. Results showed that testing women with normal cytology result using a specific 3-type HPV mRNA test can increase the screening program sensitivity without an excessive additional workload. Women with normal cytology and a negative HPV mRNA test have a very low risk of cervical cancer.
Article
Oncology
Shreya P. P. Ramkumar, Matthew C. C. Simpson, Eric Adjei Boakye, Aleksandr R. R. Bukatko, Jastin L. L. Antisdel, Sean T. T. Massa, Nosayaba Osazuwa-Peters
Summary: This study aimed to assess the independent prognostic role of human papillomavirus (HPV) status in sinonasal cancer. The results showed that HPV16/18-positive sinonasal cancer had a higher survival rate compared to HPV-negative cases, while other high-risk and low-risk HPV subtypes had similar survival rates to HPV-negative disease. Therefore, HPV status may be an important independent prognostic factor in sinonasal cancer.
Article
Virology
Zhihua Ou, Zigui Chen, Yanping Zhao, Haorong Lu, Wei Liu, Wangsheng Li, Peidi Ren, Chunyu Geng, Minfeng Xiao, Guohai Hu, Di Wu, Xiaman Wang, Na Liu, Shida Zhu, Ling Lu, Junhua Li
Summary: The study identified genetic signatures for the determination of lineage/sublineage of HPV16, 18, 52 and 58 variants, indicating heterogeneity in carcinogenicity and geographic dispersion. Surveillance in Eastern China is crucial due to the circulation of HPV variants with higher cancer risk, such as HPV16 A4 and HPV58 A3, as revealed by classifying HPV genomes obtained from Chinese females. Marker genes and signature nucleotide positions may aid in cost-effective diagnostic detections of HPV variants in clinical settings.
Article
Oncology
Jiajian Wang, Jie Dong, Yinjian Zhou, Kaiyi Wang, Min Pan, Zaixing Deng, Pengfei Wang, Ying Du, Weiguo Lu
Summary: This study aimed to assess the clinical performance of an HPV E6/E7 mRNA assay (Aptima HPV, AHPV) and AHPV 16 18/45 genotype assay (AHPV-GT) combined with age stratification for triaging women with atypical squamous cells of undetermined significance (ASC-US) cytology. The results showed that AHPV testing with age stratification is effective for triaging women with ASC-US cytology, and AHPV-GT testing may be a proper risk stratification method for women with AHPV-positive ASC-US cytology.
GYNECOLOGIC ONCOLOGY
(2022)
Article
Multidisciplinary Sciences
Ilada Choopara, Yothin Teethaisong, Narong Arunrut, Sudaluck Thunyaharn, Wansika Kiatpathomchai, Naraporn Somboonna
Summary: The study developed a simple ITS1 LAMP-HNB method for detection of fungi, demonstrating high sensitivity and applicability for preventing opportunistic fungal infections in resource-constrained areas.
Article
Pharmacology & Pharmacy
Alexandru Tirziu, Speranta Avram, Leonard Mada, Mihaela Crisan-Vida, Casiana Popovici, Dan Popovici, Cosmin Faur, Corina Duda-Seiman, Virgil Paunescu, Corina Vernic
Summary: Human papillomavirus types 16 and 18 are major causes of cervical cancers worldwide. While prophylactic vaccines based on virus-like particles (VLP) of the major capsid protein (L1) are available, they cannot eliminate existing infections. This study proposes a synthetic long peptide (SLP)-based vaccine as an alternative for infected individuals, including those with precancerous lesions. The SLP vaccine is designed to stimulate both CD8+ and CD4+ T cells, providing a strong and long-lasting immune response.
Article
Immunology
Shang-Ying Hu, Sabrina H. Tsang, Feng Chen, Qin-Jing Pan, Wen-Hua Zhang, Ying Hong, Joshua N. Sampson, Allan Hildesheim, Fang-Hui Zhao, Aimee R. Kreimer
Summary: The study found that vaginal infections are associated with incident non-HPV16/18 infections in the cervix, but do not impact the persistence of non-HPV16/18 infections after 6 months among women vaccinated with HPV16/18.
JOURNAL OF INFECTIOUS DISEASES
(2021)