Article
Oncology
Hilary A. Robbins, Aida Ferreiro-Iglesias, Tim Waterboer, Nicole Brenner, Mari Nygard, Noemi Bender, Lea Schroeder, Allan Hildesheim, Michael Pawlita, Gypsyamber D'Souza, Kala Visvanathan, Hilde Langseth, Nicolas F. Schlecht, Lesley F. Tinker, Ilir Agalliu, Sylvia Wassertheil-Smoller, Eivind Ness-Jensen, Kristian Hveem, Sara Grioni, Rudolf Kaaks, Maria-Jose Sanchez, Elisabete Weiderpass, Graham G. Giles, Roger L. Milne, Qiuyin Cai, William J. Blot, Wei Zheng, Stephanie J. Weinstein, Demetrius Albanes, Wen-Yi Huang, Neal D. Freedman, Aimee R. Kreimer, Mattias Johansson, Paul Brennan
Summary: Seropositivity for HPV16-E6 oncoprotein is a promising marker for early detection of oropharyngeal cancer (OPC), but the absolute risk of OPC after a positive or negative test is unknown. This study constructed an OPC risk prediction model and found that a substantial proportion of HPV16-E6 seropositive individuals will develop OPC.
JOURNAL OF CLINICAL ONCOLOGY
(2022)
Review
Genetics & Heredity
Christopher Rt Hillyar, Shivani S. Kanabar, Kamil R. Pufal, Alexander W. Lawson, Joshua Li Saw Hee, Kathrine S. Rallis, Anjan Nibber, Michail Sideris
Summary: This study reviewed the literature on the ability of HPV methylation biomarkers to screen for cervical precancerous and cancerous lesions and found that methylation biomarkers with high diagnostic ability have the potential to serve as independent screening tests.
Article
Microbiology
Zhi-Fang Li, Xin-Hua Jia, Xin-Yu Ren, Bei-Ke Wu, Wen Chen, Xiang-Xian Feng, Li-Bing Wang, You-Lin Qiao
Summary: This study evaluated the performance of two different principles of HPV testing in primary cervical cancer screening and ASC-US triage in rural areas. The results showed that HPV testing performed better than liquid-based cytology screening, and HPV DNA chip testing performed better than HPV PCR testing in the screening cohort.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Cell Biology
Ching-Yun Kung, Wen-Liang Fang, Yi-Ping Hung, Kuo-Hung Huang, Ming -Huang Chen, Yee Chao, Shih-Chieh Lin, Anna Fen-Yau Li, Su -Shun Lo, Chew-Wun Wu
Summary: Compared to earlier stages, stage IV gastric cancer patients showed significantly higher levels of cell-free DNA (cfDNA). This study analyzed cfDNA and tumor DNA using next-generation sequencing (NGS) and found different mutation patterns between the two. The most commonly mutated genes in tumor samples were TP53, ARID1A, KMT2C, and KMT2D, while in cfDNA samples, FAT4 and MACF1 were the most commonly mutated genes. The concordance of mutation patterns between cfDNA and tumor DNA was 42.0%. The study suggests that monitoring cfDNA mutation patterns could be useful in the treatment of stage IV gastric cancer.
Article
Infectious Diseases
Anthony Ablordey, Evans Ahotor, Charles A. Narh, Sandra A. King, Isra Cruz, Joseph M. Ndung'u, Dziedzom K. de Souza
Summary: Early diagnosis and treatment of Buruli ulcer is crucial to avoid the worsening effects of the disease. The development of new diagnostic tools like the loop-mediated isothermal amplification is encouraged. The study found that using LAMP method with primers targeting IS2404 for Buruli ulcer diagnosis has a good sensitivity and specificity, with the BURULI primer set yielding the best results.
BMC INFECTIOUS DISEASES
(2021)
Article
Oncology
Roman O. Kowalchuk, Kathryn M. Van Abel, Linda X. Yin, Joaquin Garcia, William S. Harmsen, Eric J. Moore, Daniel L. Price, Ashish Chintakuntlawar, Katharine R. Price, Scott C. Lester, Michelle Neben Wittich, Samir H. Patel, Robert L. Foote, Daniel M. Ma, Alex A. Nagelschneider, David M. Routman
Summary: The study analyzed the sensitivity and specificity of CT and PET/CT in identifying lymph node involvement and extranodal extension in patients with HPV(+) OPSCC. Results showed high accuracy for pN2 identification with CT and PET/CT, but low sensitivity for identifying pENE.
Article
Public, Environmental & Occupational Health
Ning Feng, Oliver Ezechi, Mabel Uwandu, Bowofoluwa Sharon Abimbola, Grace Deborah Vincent, Ifeoma Idigbe, Leona Chika Okoli, Mary Adesina, Jane Okwuzu, Rahaman Ademolu Ahmed, Judith Sokei, Joseph Ojonugwa Shaibu, Abidemi Esther Momoh, Omowunmi Sowunmi, Olaoniye Habeebat Labo-Popoola, Greg Ohihoin, Agatha David, Emily Nzeribe, Olufemi Olaleye, Xiao-Ping Dong, Chika Kingsley Onwuamah
Summary: This study evaluated the feasibility and performance of self-collected vaginal swab samples for HPV screening in Lagos, Nigeria. The results showed that the self-collected samples had a sensitivity of 89.80%, specificity of 98.21%, and accuracy of 96.31% compared to medic-collected samples. Self-collected sampling using real-time PCR can be a reliable method for HPV testing and may improve uptake.
Review
Oncology
Julia Hibbert, Gordana Halec, Dan Baaken, Tim Waterboer, Nicole Brenner
Summary: Serum antibodies against HPV16 early proteins, particularly E6 antibodies, have shown great promise as biomarkers for identifying HPV-driven oropharyngeal cancer. Serological assays for HPV16 have high accuracy in determining molecular HPV tumor status in OPC cases, but their performance varies depending on the molecular reference methods used.
Review
Medicine, General & Internal
Khaled Sadeq Ali Al-Shaibari, Haider Abdul-Lateef Mousa, Mohammed Abdullah A. Alqumber, Khaled A. Alqfail, AbdulHakim Mohammed, Khalid Bzeizi
Summary: This study analyzed the diagnostic performance of different clinical samples in COVID-19 detection through RT-PCR techniques. The results showed that nasopharyngeal swabs had better sensitivity but lower specificity compared to other samples. Direct comparisons between different samples and the timing of sample collection are needed to strengthen the evidence.
Article
Biochemistry & Molecular Biology
Kyung Ho Han
Summary: The study aimed to develop HPV L1 DNA reference materials for evaluating the accuracy of genotyping results. Results from HPV CHIP and RFMP assays showed high sensitivity and accuracy in detecting HPV L1 DNA. The developed reference materials by MFDS are reliable and useful for HPV genotyping assays evaluation.
Article
Biophysics
Kejun Dong, Wei Zhang, Hao Hu, Shuangshuang Cheng, Yaoqin Mu, Bei Yan, Wan Shu, Longjie Li, Hongbo Wang, Xianjin Xiao
Summary: This study innovatively invented self-amplified dual-input synthetic genetic circuit nano-vehicle, which provides a solution to the compromise between specificity and sensitivity, and demonstrated its potential application in tumor recognition and treatment through experiments.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Jiarui Zhang, Rong Zhao, Kejun Dong, Wan Shu, Sitian Wei, Yuwei Yao, Jun Zhang, Shuangge Liu, Wei Zhang, Hongbo Wang
Summary: DNA damage is a major cause of diseases, and UDG plays a crucial role in repairing DNA damage and serves as an early diagnostic marker and prognostic indicator for cancer. To meet the clinical demand, a simple, low-background, ultra-sensitive UDG activity detection method is urgently needed.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biochemical Research Methods
Jonathan D. Ogata, Wancen Mu, Eric S. Davis, Bingjie Xue, J. Chuck Harrell, Nathan C. Sheffield, Douglas H. Phanstiel, Michael Love, Mikhail G. Dozmorov
Summary: Exclusion regions are abnormal sections of reference genomes where short sequencing reads pile up. Removing reads overlapping these regions improves biological signal, particularly in differential analysis. Multiple exclusion region sets have been created by labs, primarily available through ENCODE and Github, causing uncertainty in which sets to use. Additionally, gap regions like centromeres, telomeres, and short arms pose further considerations when generating exclusion sets.
Review
Chemistry, Physical
Yidan Wang, Yunlei Xianyu
Summary: This review focuses on the applications of nanobodies and nanozymes in immunoassays. Nanobodies have advantages such as small size, high specificity, mass expression, and high stability, while nanozymes have various enzymatic activities that enhance the specificity and sensitivity of immunoassays. The challenges and outlooks in the use of nanobodies and the development of novel nanozymes in practical applications are also discussed.
Article
Oncology
Christine White, Stephen Reynolds, Katherine Murphy, Helen Keegan, Padma Naik, Roisin O'Brien, Loretto Pilkington, Imogen Sharkey Ochoa, Grainne Glesson, Noirin Russell, David Nuttall, Prerna Tewari, Fiona Wright, Sharon O'Toole, Linda Sharp, Grainne Flannelly, John J. O'Leary, Cara M. Martin
Summary: The objective of this study was to evaluate the performance of HPV mRNA and HPV DNA testing in routine cervical screening, including partial genotyping. The results showed that HPV mRNA testing demonstrated equivalent sensitivity to HPV DNA testing for detection of CIN2+ and CIN3+, but had higher specificity. HPV mRNA testing has the potential to reduce triage and follow-up tests at 12 months compared to DNA testing, with no significant difference in detection of CIN2+ and CIN3+.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Review
Pharmacology & Pharmacy
Supriyo Saha, Dilipkumar Pal, Satish B. Nimse
Summary: The study examined the development of synthetic GSK-3 inhibitors for the prevention and treatment of neurological disorders, such as Alzheimer's disease and bipolar disorders. The synthesized molecules have shown remarkable effects in combating Alzheimer's, Parkinson's depression, psychosis, and other forms of neurological disorders.
CURRENT DRUG TARGETS
(2021)
Article
Chemistry, Analytical
Satish Balasaheb Nimse, Keum-Soo Song, Shrikant Dashrath Warkad, Taisun Kim
Summary: The study introduces a new method for detecting multiple pathogens on a single platform with highly sensitive and specific DNA hybridization. Experimental results demonstrate that the method can efficiently detect and discriminate in clinical samples.
Review
Chemistry, Medicinal
Supriyo Saha, Dilipkumar Pal, Satish Balasaheb Nimse
Summary: This manuscript focuses on the importance of indazole derivatives in the treatment of various gastrointestinal diseases. Different indazole derivatives play a critical role in the treatment of different diseases such as IBS, diarrhea, metabolic syndrome, and gastrointestinal cancers. By analyzing the receptors associated with gastrointestinal disorders, it was found that indazole derivatives can effectively interact with the receptors.
CURRENT TOPICS IN MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Medicinal
Jung-Seop Lee, In-ho Song, Shrikant Dashrath Warkad, Gyu Seong Yeom, Pramod B. Shinde, Keum-soo Song, Satish Balasaheb Nimse
Summary: In this study, a series of 2,5-substituted-1H-benzo[d]imidazole derivatives were synthesized and it was demonstrated that compounds B15, B16, B19, and B20 exhibit excellent anticancer activity.
DRUG DEVELOPMENT RESEARCH
(2022)
Article
Chemistry, Analytical
Keum-Soo Song, Satish Balasaheb Nimse, Shrikant Dashrath Warkad, Jung-Hoon Kim, Hey-Jin Kim, Taisun Kim
Summary: Lung cancer is a leading cause of death globally. Low-dose computed tomography (LDCT) reduces lung cancer mortality by 20% compared to standard chest X-rays among current or heavy smokers, but has a high false-positive rate. This study presents a method based on biomarker detection to complement LDCT for detecting various stages of lung cancer.
Article
Biochemistry & Molecular Biology
Dilipkumar Pal, In-ho Song, Shrikant Dashrath Warkad, Keum-soo Song, Gyu Seong Yeom, Supriyo Saha, Pramod B. Shinde, Satish Balasaheb Nimse
Summary: This review presents the research advances on compounds containing indazole scaffolds as microtubule targeting agents based on articles published in the last two decades. Several compounds have shown potent microtubule inhibition and anticancer activity, with some of them having the lowest IC50 values observed in cell line studies.
BIOORGANIC CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Supriyo Saha, Gyu Seong Yeom, Satish Balasaheb Nimse, Dilipkumar Pal
Summary: The manuscript focuses on providing clues to enhance the innate immunity of coronavirus patients and protect them from new mutant strains and black fungus infections. Through molecular docking studies, potential therapeutic candidates for COVID-19 and drugs for treating black fungus infections were identified. The combined therapy of ledipasvir and itraconazole could be a better alternative for treating COVID-19 patients coinfected with black fungus.
BIOMED RESEARCH INTERNATIONAL
(2022)
Article
Chemistry, Analytical
In-ho Song, Gyu Seong Yeom, Anil Kuwar, Satish Balasaheb Nimse
Summary: In this study, a benzimidazole-based compound and its derivative probe were synthesized for the highly selective detection and quantification of cysteine in human serum. The derivative probe showed high selectivity and sensitivity for cysteine and achieved lower detection limits compared to reported methods in serum samples.
Article
Biochemistry & Molecular Biology
Kundan Tayade, Gyu-Seong Yeom, Suban K. Sahoo, Horst Puschmann, Satish Balasaheb Nimse, Anil Kuwar
Summary: This study synthesized Schiff base compound 2 and determined its crystal structure and molecular structure. The antioxidant activity of this compound was confirmed through calculations and experiments. The compound exhibited excellent antioxidant activity and low cytotoxicity.
Article
Chemistry, Analytical
Prasad M. Sonawane, Neha Jain, Arkaprava Roychaudhury, Su Jeong Park, Vikas K. Bhosale, Mahesh B. Halle, Cheol-Hee Kim, Satish Balasaheb Nimse, David G. Churchill
Summary: This study introduces the synthesis and application of a highly sensitive fluorescence turn-on probe, Myco-OCl, for the specific and rapid detection of HOCl. The probe shows exceptional performance and has been successfully applied for live cell imaging and detection of HOCl in a zebrafish model.
Article
Chemistry, Analytical
Prasad M. Sonawane, Neha Jain, Arkaprava Roychaudhury, Su Jeong Park, Vikas K. Bhosale, Mahesh B. Halle, Cheol-Hee Kim, Satish Balasaheb Nimse, David G. Churchill
Summary: This study introduces a highly sensitive fluorescence turn-on probe, Myco-OCl, for detecting HOCl with exceptional specificity and rapid response. The probe has great potential for detecting HOCl in diverse physiological systems.
Article
Chemistry, Medicinal
Keum-soo Song, Satish Balasaheb Nimse, Junghoon Kim, Shrikant Dashrath Warkad, Taisun Kim
Summary: The developed ID-Checker enables highly specific delivery of anticancer agents to cancer cells through GLUT channels, resulting in cancer cell death within a short period of time without causing damage to normal cells.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Manohar Patil, Su Jeong Park, Gyu Seong Yeom, Ratnamala Bendre, Anil Kuwar, Satish Balasaheb Nimse
Summary: A Schiff base ligand FHE was synthesized and showed specific complex formation with Zn2+ ions, resulting in enhanced fluorescence. FHE demonstrated high sensitivity for detecting Zn2+ ions in living cells and environmental samples.
NEW JOURNAL OF CHEMISTRY
(2022)
Correction
Materials Science, Multidisciplinary
In-ho Song, Pritam Torawane, Jung-Seop Lee, Shrikant Dashrath Warkad, Amulrao Borase, Suban K. Sahoo, Satish Balasaheb Nimse, Anil Kuwar
Summary: The study successfully detected Al3+ and Cu2+ ions using isonicotinohydrazide-based chemosensors and applied them to live-cell imaging.
MATERIALS ADVANCES
(2021)
Article
Materials Science, Multidisciplinary
In-ho Song, Pritam Torawane, Jung-Seop Lee, Shrikant Dashrath Warkad, Amulrao Borase, Suban K. Sahoo, Satish Balasaheb Nimse, Anil Kuwar
Summary: The newly synthesized Schiff base receptor 3 exhibits excellent selectivity and sensitivity towards Cu2+ and Al3+ ions through UV-vis absorption and fluorescence spectroscopy, respectively. It shows detectable color changes and significant fluorescent enhancement in the presence of these ions, with a much lower limit of detection for Al3+ ions.
MATERIALS ADVANCES
(2021)