Article
Microbiology
Ryota Miyazawa, So Shimoda, Keiichi Matsuda, Ryuta Tobe, Tasuke Ando, Hiroshi Yoneyama
Summary: Staphylococcus aureus is a major pathogen in humans and livestock, causing serious issues such as bovine mastitis. This study monitored cases of intramammary infection caused by S. aureus in the Miyagi Prefecture, Japan, and analyzed the strains obtained from dairy farms. The study found recurrent cases of infection and identified a Methicillin-Sensitive SA (MSSA) ST398 strain isolated from mastitis-containing cows, which is the first confirmed case in Japan. The results highlight the importance of nationwide surveillance to manage the distribution of ST398 strains in dairy farms for human and animal health.
Article
Agriculture, Dairy & Animal Science
H. Reydams, A. Wuytack, S. Piepers, K. Mertens, F. Boyen, F. N. de Souza, F. Haesebrouck, S. De Vliegher
Summary: This study assessed the genetic diversity of S. hominis isolated from different habitats and investigated their ability to utilize ferritin and lactoferrin as iron sources. The results showed that S. hominis strains can utilize ferritin to reverse the growth inhibition caused by iron deprivation.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Veterinary Sciences
Muhammad Umar Javed, Muhammad Ijaz, Zahida Fatima, Aftab Ahmad Anjum, Amjad Islam Aqib, Muhammad Muddassir Ali, Abdul Rehman, Arslan Ahmed, Awais Ghaffar
Summary: The study conducted in bovines of district Faisalabad, Pakistan aimed to investigate the phenotypic prevalence of MRSA and VRSA in milk samples positive for subclinical mastitis, and to assess the associated risk factors and antimicrobial susceptibility pattern against MRSA and VRSA isolates.
PAKISTAN VETERINARY JOURNAL
(2021)
Article
Microbiology
Jiun-Liang Chen, Tsung-Yu Huang, Wei-Bin Hsu, Chiang-Wen Lee, Yao-Chang Chiang, Pey-Jium Chang, Kuo-Ti Peng
Summary: This study investigated the genotypic and phenotypic characteristics of MRSA clones isolated from PJI patients in a hospital in Taiwan. The findings showed that ST239 MRSA isolates were highly resistant to multiple antibiotics, had a high biofilm formation ability, and infected patients typically exhibited fever and inflammation. Additionally, knee infections were mainly attributed to ST59 MRSA, and there was an increasing trend of infections caused by ST8 and other MRSA genotypes in PJI patients.
Article
Microbiology
Abdeljallil Zeggay, Alban Atchon, Benoit Valot, Didier Hocquet, Xavier Bertrand, Kevin Bouiller
Summary: Using genomic data, the study determined the origin of MRSA ST398 isolates causing invasive infections in patients with no livestock contact. The isolates were sequenced and analyzed for the presence of virulence and resistance genes. Phylogenetic analysis revealed that MSSA isolates were human-associated, while MRSA isolates were livestock-associated. The results suggest that livestock-associated MRSA acquired virulence genes, enabling them to cause invasive infections in humans.
Article
Food Science & Technology
Basavaprabhu Haranahalli Nataraj, Chette Ramesh, Rashmi Hogarehalli Mallappa
Summary: Metabolites of lactic acid bacteria have potential as postbiotics to inhibit the growth of undesirable microorganisms in food and pharmaceutical industries. The biosurfactants extracted from Lactobacillus acidophilus NCFM and Lacticaseibacillus rhamnosus GG showed promising antimicrobial and antibiofilm activities against staphylococcal isolates, indicating their potential as candidates for further technology intervention.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ramamoorthy Sivakumar, Parameswaran Sree Pranav, Madhavi Annamanedi, S. Chandrapriya, Shrikrishna Isloor, Jeyaprakash Rajendhran, Nagendra R. R. Hegde
Summary: This study conducted a comprehensive analysis of bovine mastitis-associated S. aureus isolates from India using whole genome sequencing and comparative genomic analysis. The results revealed 15 different sequence types and 5 clonal complexes. The genome of Indian mastitis-associated S. aureus strains is relatively closed. Phylogenetic analysis based on SNP variation differentiated the 41 strains into six major clades. Seventeen different antimicrobial resistance genes and 108 virulence-associated genes were identified among these isolates.
Article
Food Science & Technology
Xiaonan Zhao, Xiaomeng Yuan, Ming Hu, Yin Zhang, Lulu Li, Qing Zhang, Xuexia Yuan, Wenbo Wang, Yuqing Liu
Summary: The study revealed a relatively high prevalence of S. aureus in bulk tank milk from Shandong dairy farms, with the isolates carrying multiple virulence genes and exhibiting multiple antimicrobial resistance. The lurking threat of MRSA in raw milk emphasizes the need for surveillance programs starting at the farm level.
Article
Multidisciplinary Sciences
Nathita Phumthanakorn, Sybille Schwendener, Valentina Dona, Pattrarat Chanchaithong, Vincent Perreten, Nuvee Prapasarakul
Summary: This study conducted genomic comparative analyses of MRSP isolates from dogs and humans, revealing differences in prophage distribution and identification of a novel composite pathogenicity island in ST181 isolates. Among the different STs, ST45 isolates showed the highest genomic diversity, suggesting potential host adaptation leading to different colonization patterns.
Article
Biology
Hassan A. Hemeg
Summary: The study found that companion animals such as dogs and cats may serve as a potential reservoir for MRSA, with genetic similarity between strains recovered from these animals and their human owners, indicating that pets play an important role in the transmission and colonization of MRSA.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Immunology
Philip Nikolic, Poonam Mudgil, David G. Harman, John Whitehall
Summary: This study aimed to identify and compare the general differences in protein profiles among clinical strains of S. aureus sensitive and resistant to methicillin. The proteomic analysis revealed differences in protein abundances related to virulence, metabolism, and protein synthesis between MSSA and MRSA strains.
MICROBIAL PATHOGENESIS
(2023)
Article
Infectious Diseases
Tahir Lubna, Tahir Hussain, Ashwag Shami, Naseem Rafiq, Shehryar Khan, Muhammad Kabir, Naimat Ullah Khan, Irfan Khattak, Mustafa Kamal, Tahir Usman
Summary: The main objectives of this study were to determine the antimicrobial resistance pattern of S. aureus in raw milk and to detect the presence of mecA and tetK genes in it. Out of the 150 milk samples tested, S. aureus was detected in 55 (37%) of them, with 11 of them being multidrug-resistant strains. The highest resistance was found to penicillin (100%) and oxacillin (100%), while amoxicillin and ciprofloxacin were found to be susceptible (100%). The presence of mecA and tetK genes was detected in 9 and 7 of the multidrug-resistant S. aureus isolates, respectively.
Article
Infectious Diseases
Desiree Corvera Klove, Vibeke Frokjaer Jensen, Laerke Boye Astrup
Summary: This study reports the first case of MRSA t304/ST6 variant found in bovine clinical mastitis. The variant, which is rare in livestock but widely recognized in humans, showed low susceptibility to cefoxitin and carried the mecA resistance gene. The study also detected potential virulence genes associated with human specificity.
Article
Agriculture, Dairy & Animal Science
Valerie E. Ryman, Felicia M. Kautz, Steve C. Nickerson
Summary: Accurate identification of Staphylococcus aureus is crucial in managing mastitis in dairy cattle, but simple laboratory techniques may lead to misidentifications. A study conducted on a dairy farm in Georgia revealed that almost 64% of isolates initially deemed to be non-aureus isolates were actually S. aureus.
Article
Multidisciplinary Sciences
Keming Ning, Rushun Zhou, Manxiang Li
Summary: This study analyzed the capsular polysaccharides, molecular typing, and antibiotic resistance characteristics of Staphylococcus aureus isolates from raw milk of dairy farms in Hunan Province, China. The results revealed the presence of CP5 (42.10%) and CP8 (57.89%) genes in the isolates. The isolates showed a multiple antimicrobial resistance rate of 26.75%, with the highest resistance rate to penicillin (82.89%). Molecular typing identified 22 sequence types (ST) and 20 spa types, with t796-ST7 being the most prevalent, possibly indicating the major epidemic strain of multidrug-resistant S. aureus.