4.7 Article

False-Positive Transcription-Mediated Amplification Assay Detection of West Nile Virus in Blood from a Patient with Viremia Caused by an Usutu Virus Infection

期刊

JOURNAL OF CLINICAL MICROBIOLOGY
卷 48, 期 9, 页码 3338-3339

出版社

AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.02501-09

关键词

-

向作者/读者索取更多资源

Detection of West Nile virus (WNV) by nucleic acid amplification technology (NAAT) is used widely to screen blood and organ donations in areas where WNV is endemic. We report a false-positive result of a WNV transcription-mediated amplification assay (TMA) in a patient with viremia that was caused by Usutu virus, a mosquito-borne flavivirus.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

Article Infectious Diseases

Critically ill patients with COVID-19 show lung fungal dysbiosis with re duce d microbial diversity in patients colonized with Candida spp.

Elisa Viciani, Paolo Gaibani, Andrea Castagnetti, Andrea Liberatore, Michele Bartoletti, Pierluigi Viale, Tiziana Lazzarotto, Simone Ambretti, Russell Lewis, Monica Cricca

Summary: This study aimed to compare the lower respiratory tract mycobiome of critically ill patients with COVID-19 to patients without COVID-19 pneumonia. The results showed that COVID-19 patients were more likely to be colonized with Candida spp., and ARDS was associated with lung dysbiosis.

INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES (2022)

Article Virology

Viral Population Heterogeneity and Fluctuating Mutational Pattern during a Persistent SARS-CoV-2 Infection in an Immunocompromised Patient

Martina Brandolini, Silvia Zannoli, Giulia Gatti, Valentina Arfilli, Monica Cricca, Giorgio Dirani, Agnese Denicolo, Simona Semprini, Laura Grumiro, Manuela Imola, Damiano Larne, Maria Michela Marino, Martina Manera, Andrea Mancini, Francesca Taddei, Manuel Zagarrigo, Carlo Biagetti, Vittorio Sambri

Summary: This study aims to provide further insight into the evolutionary dynamics of SARS-CoV-2 by analyzing a patient with follicular lymphoma who developed a persistent infection for over 7 months. The analysis revealed the evolution of genetically similar subpopulations within the host, indicating that prolonged replication is associated with intra-host virus evolution. These findings support the hypothesis that SARS-CoV-2 can undergo adaptive evolution in immunocompromised patients.

VIRUSES-BASEL (2023)

Review Oncology

Microbiota in the Natural History of Pancreatic Cancer: From Predisposition to Therapy

Cecilia Binda, Giulia Gibiino, Monica Sbrancia, Chiara Coluccio, Maria Cazzato, Lorenzo Carloni, Alessandro Cucchetti, Giorgio Ercolani, Vittorio Sambri, Carlo Fabbri

Summary: Pancreatic cancer still has a poor prognosis, despite advancements in diagnosis and surgical management. The gut microbiome is gaining interest in its role in the development and management of pancreatic cancer. This review aims to provide a comprehensive understanding of the gut microbiome's role in the history of pancreatic cancer for researchers and clinicians.

CANCERS (2023)

Article Microbiology

Omicron Sub-Lineage BA.5 and Recombinant XBB Evasion from Antibody Neutralisation in BNT162b2 Vaccine Recipients

Martina Brandolini, Giulia Gatti, Laura Grumiro, Silvia Zannoli, Valentina Arfilli, Monica Cricca, Giorgio Dirani, Agnese Denicolo, Maria Michela Marino, Martina Manera, Andrea Mancini, Francesca Taddei, Simona Semprini, Vittorio Sambri

Summary: The recent emergence of new SARS-CoV-2 variants resulting from recombination has raised concerns about enhanced infectivity and immune escape. XBB, a recombinant of BA.2.10 and BA.2.75, has rapidly increased in prevalence and showed enhanced immune escape ability compared to ancestral lineage and another co-circulating variant. This study highlights the importance of monitoring immune escape and reformulating vaccines for new and forthcoming variants.

MICROORGANISMS (2023)

Article Infectious Diseases

A novel method to evaluate ceftazidime/avibactam therapy in patients with carbapenemase-producing Enterobactericeae (CPE) bloodstream infections

Milo Gatti, Vincent H. Tam, Paolo Gaibani, Pier Giorgio Cojutti, Pierluigi Viale, Federico Pea

Summary: This study reports the management of eight patients with bloodstream infections using a continuous infusion of ceftazidime-avibactam. The results show that patients with a lower ceftazidime concentration compared to the minimum inhibitory concentration have a higher risk of microbiological failure and development of resistance.

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS (2023)

Article Neurosciences

Case report of Usutu virus infection in an immunocompromised patient in Italy, 2022

Paolo Gaibani, Nicole Barp, Marco Massari, Emanuele Alberto Negri, Giada Rossini, Caterina Vocale, Chiara Trenti, Altea Gallerani, Samuele Cantergiani, Federico Romani, Mattia Simion, Cristina Mussini, Tiziana Lazzarotto

Summary: USUV is an arthropod-borne flavivirus that emerged in Africa in the 1950s and in Europe in the 1990s, causing a high number of bird deaths. Recently, it has been hypothesized that USUV can also infect humans, although cases are limited and mostly in immunocompromised individuals. This report describes a case of USUV meningoencephalitis in an immunocompromised patient with no history of previous flavivirus infection. The infection progressed rapidly and resulted in fatal outcomes in just a few days after symptoms onset, potentially due to a co-infection with bacteria. This finding suggests that careful attention should be paid to neurological syndromes, especially during summer months, among immunocompromised patients in countries endemic to USUV.

JOURNAL OF NEUROVIROLOGY (2023)

Article Infectious Diseases

Complete genome sequence and antimicrobial resistance analysis of ESBL-producing Shigella sonnei carrying small cryptic plasmids isolated in northern Italy

Stefano Amadesi, Simone Ambretti, Tiziana Lazzarotto, Paolo Gaibani

Summary: Using a hybrid approach that combined Oxford Nanopore Technologies and Illumina platforms, we sequenced and assembled the genome of a Shigella sonnei isolate carrying several small plasmids. Whole-genome sequencing was conducted using the Illumina iSeq 100 and Oxford Nanopore MinION systems, and the resulting reads were used for hybrid genome assembly via Unicycler. Our findings revealed the presence of antimicrobial resistance genes in small cryptic plasmids, which may represent an overlooked mechanism for the propagation of these genes among bacterial populations.

JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE (2023)

Article Pharmacology & Pharmacy

An Enhanced Dissolving Cyclosporin-A Inhalable Powder Efficiently Reduces SARS-CoV-2 Infection In Vitro

Davide D'Angelo, Eride Quarta, Stefania Glieca, Giada Varacca, Lisa Flammini, Simona Bertoni, Martina Brandolini, Vittorio Sambri, Laura Grumiro, Giulia Gatti, Giorgio Dirani, Francesca Taddei, Annalisa Bianchera, Fabio Sonvico, Ruggero Bettini, Francesca Buttini

Summary: This study developed a dry inhalation powder of cyclosporine-A for lung transplantation rejection prevention and COVID-19 treatment. The impact of excipients on the powder's critical quality attributes was investigated. The optimized powder exhibited faster dissolution, better respirability, and showed efficacy in reducing IL-6 and inhibiting SARS-CoV-2 replication, suggesting its potential as a therapeutic strategy for lung rejection prevention and COVID-19 treatment.

PHARMACEUTICS (2023)

Article Infectious Diseases

Synergistic Activity of Cefiderocol in Combination with Piperacillin-Tazobactam, Fosfomycin, Ampicillin-Sulbactam, Imipenem-Relebactam and Ceftazidime-Avibactam against Carbapenem-Resistant Gram-Negative Bacteria

Marta Palombo, Federica Bovo, Stefano Amadesi, Paolo Gaibani

Summary: Limited treatment options contribute to the emergence of antimicrobial resistance, and new therapeutic agents are needed for the treatment of certain pathogens. The combination of antibiotics is an effective strategy to combat multidrug-resistant infections.

ANTIBIOTICS-BASEL (2023)

Article Infectious Diseases

Comparison of Broth Microdilution, Disk Diffusion and Strip Test Methods for Cefiderocol Antimicrobial Susceptibility Testing on KPC-Producing Klebsiella pneumoniae

Federica Bovo, Tiziana Lazzarotto, Simone Ambretti, Paolo Gaibani

Summary: The aim of this study was to compare the BMD method with the DD test and strip test in assessing the antimicrobial activity of CFD against KPC-Kp strains. The CA between the BMD method and DD test was 92%, while the CA between the BMD method and strip test was 90.7%. KPC-Kp strains showing resistance to CFD were 36% by three methods. The DD test was found to be a valid alternative to the BMD method, while the strip test exhibited major limitations.

ANTIBIOTICS-BASEL (2023)

Article Microbiology

Could an optimized joint pharmacokinetic/pharmacodynamic target attainment of continuous infusion ceftazidime-avibactam be a way to avoid the need for combo therapy in the targeted treatment of deep-seated DTR Gram-negative infections?

Milo Gatti, Matteo Rinaldi, Cecilia Bonazzetti, Paolo Gaibani, Maddalena Giannella, Pierluigi Viale, Federico Pea

Summary: This study aimed to assess the relationship between joint pharmacokinetic/pharmacodynamic (PK/PD) target attainment of continuous infusion ceftazidime-avibactam and the microbiological outcome of difficult-to-treat resistant Gram-negative infections. The study found that optimized joint PK/PD target attainment could be a way to eradicate these infections and render combination therapy unnecessary.

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY (2023)

Article Infectious Diseases

Pharmacokinetics/pharmacodynamics of cefiderocol administered by continuous infusion in a case series of critically ill patients with carbapenem-resistant Acinetobacter baumannii infections undergoing continuous venovenous haemodiafiltration (CVVHDF)

Milo Gatti, Matteo Rinaldi, Tommaso Tonetti, Paolo Gaibani, Antonio Siniscalchi, Pierluigi Viale, Federico Pea

Summary: This study aimed to evaluate the pharmacokinetics/pharmacodynamics of cefiderocol administered by continuous infusion in critically ill patients. The results showed that continuous infusion could achieve therapeutic targets for severe Acinetobacter baumannii infections, especially in patients undergoing high-intensity continuous venovenous haemodiafiltration and with residual diuresis.

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS (2023)

Letter Infectious Diseases

A descriptive pharmacokinetic/pharmacodynamic analysis of continuous infusion meropenem/vaborbactam in the treatment of critically ill patients with documented KPC-producing Klebsiella pneumoniae ventilator-associated pneumonia

Milo Gatti, Matteo Rinaldi, Paolo Gaibani, Antonio Siniscalchi, Tommaso Tonetti, Pierluigi Viale, Federico Pea

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS (2023)

Article Virology

Genomic and Temporal Analysis of Deletions Correlated to qRT-PCR Dropout in N Gene in Alpha, Delta and Omicron Variants

Giulia Gatti, Martina Brandolini, Andrea Mancini, Francesca Taddei, Silvia Zannoli, Giorgio Dirani, Martina Manera, Valentina Arfilli, Agnese Denicolo, Anna Marzucco, Maria Sofia Montanari, Irene Zaghi, Massimiliano Guerra, Rita Tennina, Maria Michela Marino, Laura Grumiro, Monica Cricca, Vittorio Sambri

Summary: Since the first SARS-CoV-2 outbreak, mutations have changed the viral genome sequence, structure, and protein folding, leading to the onset of new variants. These alterations challenge both the clinical field and diagnostic demand due to detection failures and incomplete PCR results. Analyzing understudied genes such as N and investigating regions prone to mutation through WG-NGS can help identify new or reacquired mutations for designing robust primers.

VIRUSES-BASEL (2023)

Article Biochemistry & Molecular Biology

Whole Genome Sequencing of a Chlamydia trachomatis Strain Responsible for a Case of Rectal Lymphogranuloma Venereum in Italy

Antonella Marangoni, Stefano Amadesi, Marielle Ezekielle Djusse, Claudio Foschi, Valeria Gaspari, Tiziana Lazzarotto, Paolo Gaibani

Summary: Lymphogranuloma venereum (LGV) is a systemic sexually transmitted infection mainly spreading within men who have sex with men (MSM). In this study, the whole genome of a C. trachomatis strain (LGV/17) responsible for a rectal LGV case was described. The LGV/17 strain showed a genomic structure similar to reference sequences and was closely related to other L2f strains, indicative of the long-distance dynamics of transmission.

CURRENT ISSUES IN MOLECULAR BIOLOGY (2023)

暂无数据